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The map label for this gene is xapA [H]

Identifier: 218531142

GI number: 218531142

Start: 3373123

End: 3373935

Strand: Reverse

Name: xapA [H]

Synonym: Mchl_3192

Alternate gene names: 218531142

Gene position: 3373935-3373123 (Counterclockwise)

Preceding gene: 218531143

Following gene: 218531141

Centisome position: 58.39

GC content: 73.06

Gene sequence:

>813_bases
ATGGCACCTGACGACGCGGCCATCGCCCCTCTGCGCGCGGCAGGCTTTTCCGGGCCCTATGCCCTGGCGATCGTCACGGG
GACTGGGCTCGGCGCCCTGGTCGAGGCGGTCGCGGATCGTGTCAGCCTCGATTATGGGGCGATTCCCGGCTTTCCCGCCC
CCGGCGTCAGCGGCCATGGCGGGCGGCTGCATCGCGGGCGCCTGTCGGGGCGGCCGGTGCTCGTGTTCGAGGGGCGGACC
CACGCCTACGAGACCGGCGACGCTGCCGCCATGCGGGTGCCGCTCGCTGCCGCGCGTGCGCTCGGCGCGGCGCTGCTGCT
GCTCACCAACGCCGCCGGTTCGCTGCGCCCGGAGATCGGCCCCGGCCGCCTCGTGATGCTGGCTGACCACATCAACTTGT
CCGGCCTCAATCCCCTGATCGGGGAGCCGAGCGACGCCCGCTTCGTCTCGATGACGGAGGCCTACGATCCGGGCCTGCGC
GCTCACCTGAGTGCGGCGGCCGAGGCAATCGGGCTGCCGCTGCCTGCCGGCGTCTACGCATGGTTCTCGGGCCCAAGTTT
CGAGACGCCGGCCGAGGTGCGCATGGCCGGGATTCTCGGCGCCGACCTCGTCGGCATGTCCACCGTGCCGGAAACGATCC
TCGCCCGGTTCCTCGGCATGCCGGTGGCGGCGATCTCCGTGGTGACGAACCTCGCGGCGGGGATCGCGGGCGGAGACCCG
CACCATGCCGAAACCAAGGCGGTCGCCGCCCGCGCCGGGGCGGATCTCGCCCGGCTCGTCACCGGCTTCGTCGGCCGGCT
GCCCGAAGCGTAG

Upstream 100 bases:

>100_bases
CCCGACACGCCGATCGTCTCGGCCGGTCCCGACGGCGTGCGGGCGCGCTACACGCTGGAGCAATTGCTGCCCGCCTCCTT
CGGCCCGGAGCATTTCGTCC

Downstream 100 bases:

>100_bases
GAAACACGGATGACCGCTTCAGCCCCCCTCCCCCCGCAGGATGCGGTCCGAGTCAGGGCGGATCTGGCGCGACGCGCCCT
GCCGCTGCTCGATCTCACCG

Product: purine nucleoside phosphorylase

Products: purine; alpha-D-ribose 1-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAPDDAAIAPLRAAGFSGPYALAIVTGTGLGALVEAVADRVSLDYGAIPGFPAPGVSGHGGRLHRGRLSGRPVLVFEGRT
HAYETGDAAAMRVPLAAARALGAALLLLTNAAGSLRPEIGPGRLVMLADHINLSGLNPLIGEPSDARFVSMTEAYDPGLR
AHLSAAAEAIGLPLPAGVYAWFSGPSFETPAEVRMAGILGADLVGMSTVPETILARFLGMPVAAISVVTNLAAGIAGGDP
HHAETKAVAARAGADLARLVTGFVGRLPEA

Sequences:

>Translated_270_residues
MAPDDAAIAPLRAAGFSGPYALAIVTGTGLGALVEAVADRVSLDYGAIPGFPAPGVSGHGGRLHRGRLSGRPVLVFEGRT
HAYETGDAAAMRVPLAAARALGAALLLLTNAAGSLRPEIGPGRLVMLADHINLSGLNPLIGEPSDARFVSMTEAYDPGLR
AHLSAAAEAIGLPLPAGVYAWFSGPSFETPAEVRMAGILGADLVGMSTVPETILARFLGMPVAAISVVTNLAAGIAGGDP
HHAETKAVAARAGADLARLVTGFVGRLPEA
>Mature_269_residues
APDDAAIAPLRAAGFSGPYALAIVTGTGLGALVEAVADRVSLDYGAIPGFPAPGVSGHGGRLHRGRLSGRPVLVFEGRTH
AYETGDAAAMRVPLAAARALGAALLLLTNAAGSLRPEIGPGRLVMLADHINLSGLNPLIGEPSDARFVSMTEAYDPGLRA
HLSAAAEAIGLPLPAGVYAWFSGPSFETPAEVRMAGILGADLVGMSTVPETILARFLGMPVAAISVVTNLAAGIAGGDPH
HAETKAVAARAGADLARLVTGFVGRLPEA

Specific function: The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=259, Percent_Identity=38.996138996139, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI47132622, Length=143, Percent_Identity=34.2657342657343, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788746, Length=249, Percent_Identity=42.9718875502008, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17541190, Length=255, Percent_Identity=36.4705882352941, Blast_Score=153, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6323238, Length=282, Percent_Identity=34.0425531914894, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45552887, Length=259, Percent_Identity=34.3629343629344, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24656090, Length=259, Percent_Identity=34.3629343629344, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24656093, Length=256, Percent_Identity=34.765625, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45552885, Length=256, Percent_Identity=34.765625, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24762376, Length=244, Percent_Identity=28.6885245901639, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221459247, Length=180, Percent_Identity=32.2222222222222, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR010943
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.1

Molecular weight: Translated: 27214; Mature: 27082

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPDDAAIAPLRAAGFSGPYALAIVTGTGLGALVEAVADRVSLDYGAIPGFPAPGVSGHG
CCCCCCCCCCHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GRLHRGRLSGRPVLVFEGRTHAYETGDAAAMRVPLAAARALGAALLLLTNAAGSLRPEIG
CCEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGRLVMLADHINLSGLNPLIGEPSDARFVSMTEAYDPGLRAHLSAAAEAIGLPLPAGVYA
CCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCEEE
WFSGPSFETPAEVRMAGILGADLVGMSTVPETILARFLGMPVAAISVVTNLAAGIAGGDP
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
HHAETKAVAARAGADLARLVTGFVGRLPEA
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
APDDAAIAPLRAAGFSGPYALAIVTGTGLGALVEAVADRVSLDYGAIPGFPAPGVSGHG
CCCCCCCCCHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GRLHRGRLSGRPVLVFEGRTHAYETGDAAAMRVPLAAARALGAALLLLTNAAGSLRPEIG
CCEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PGRLVMLADHINLSGLNPLIGEPSDARFVSMTEAYDPGLRAHLSAAAEAIGLPLPAGVYA
CCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCEEE
WFSGPSFETPAEVRMAGILGADLVGMSTVPETILARFLGMPVAAISVVTNLAAGIAGGDP
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
HHAETKAVAARAGADLARLVTGFVGRLPEA
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: purine nucleoside; phosphate

Specific reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559336; 9205837; 9278503 [H]