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The map label for this gene is pyk

Identifier: 218530921

GI number: 218530921

Start: 3133430

End: 3134866

Strand: Reverse

Name: pyk

Synonym: Mchl_2971

Alternate gene names: 218530921

Gene position: 3134866-3133430 (Counterclockwise)

Preceding gene: 218530923

Following gene: 218530904

Centisome position: 54.26

GC content: 69.1

Gene sequence:

>1437_bases
TTGAAGCGCTCGCGCCGCACCAAGATCGTCGCTACCCTCGGCCCGGCATCCGATACGCCGGAGATGATCGAGAAGCTGTT
CCACGCCGGTGCGGACGTGTTCCGCATCAACATGAGCCATCTGGCTCGCGAGAAACTGCCCGAACGCATCGAGGTGATTC
GCACCATCGAGCGCGAGGCCAAGCGCCCCATCGGCATCCTCGTGGACCTTCAGGGCCCGAAGCTGCGGCTCGGCACCTTC
GTGGGCGACGCGGCCGTGCTGGAGAACGGCCAGACCTTCGTGCTCGATTCGGATCCGACGCCGGGCGATACCGACCGCGT
CTTCCTTCCCCATCCCGAGATCCTGTCGGCGCTGGAGCCCAGCCACGGCATCCTGATCGACGACGGCAAGCTTCGCCTCA
TCGTCACCGAGGTGAGCGAGGGGCGTGCCGTCACCCGCGTCGAGGTCGGCGGGCGCATCTCGAATCGCAAGGGCGTGTCG
CTGCCTCACACCGTGCTGCCGGTGCCGGCGATGACCGAGAAGGATCGCGGAGATCTGGAAGCGGGCCTCGCGGCCGGCGC
CGACTGGATCGCGGTGTCCTTCGTGCAGCGCCCGGAAGACGTGGCGGAGGTCAAAAAGGTCGCCGCCGGGCGTGCCCTGG
TGATGGCCAAGATCGAGAAGCCGCAGGCGCTCACCCGCCTCGACGAGATCATCGAGATCTCCGACGGCATCATGGTCGCC
CGCGGCGACCTCGGCGTCGAGATGCCGCTCGAACAGGTGCCGGGCGTGCAGAAGCGCATCACCCGCGTCGCCCGCCGGCT
CGGCAAGCCGGTCGTCGTCGCTACGCAGATGCTCGAATCGATGATCACCTCACCGGTGCCGACCCGCGCCGAGGTGTCGG
ACGTGGCCACCGCCGTCTACGAGGGCGCGGATGCGGTGATGCTCTCGGCCGAAAGCGCCGCCGGCGACTTTCCGGTCGAG
GCGATTGGCACCATGAACCGCATCGCCGAGCAGGTGGAGCGCGACGCGCTCTACTGGTCGATCCTGATGGCGCAGCGTTC
CGAGCCGGAGCCGACGGCCTCCGACGCCATCGCGGCCGCTGCCCACCAGATCGTCGAGGCGCTGAGCCTGCGCTCGATCA
TGGCCTGGACCCATTCCGGTTCGACGGTGCTGCGGCTGGCGCGCGCCCGGCCCAATGCCAGCGTCATCGCGCTGACGCCG
AAGCGCGAGACGGCGCGGCGCCTCACCATGGCCTGGGGCGTGCACCCGATCGTCACCAAGGATGCCAGCGACGTGGACGA
CATGGCCTTCCGCGCCGCGAAGTTCGCGGTGCGCGAGCGCTTCGCCGAGATCGGCGACCGGGTGATCATCGTGGCCGGCG
TGCCGTTCGGCATTCCCGGGGCGACCAACATGGTGCGCATCGCCTTCGTCACCCGCGAGCACGCCGAGCGGGCGTAG

Upstream 100 bases:

>100_bases
TCCGGCCATCGGCGCCCGCCCGCGCGCCACCCATCCGCCGGGCCGTTCCCGCACGCGGTCGCCGCGCCTCGCGGGTCCAG
CGGGTATGATGGAGGCCCCT

Downstream 100 bases:

>100_bases
AACAGCGTCCCGGATATCGGATCCGGGTGGACGCTCGATCGCGCGGAAGCTCTACCGCGCGATCGTCGCCGTCAACGCCT
CGACCTCGCGCACGAGCGTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKRSRRTKIVATLGPASDTPEMIEKLFHAGADVFRINMSHLAREKLPERIEVIRTIEREAKRPIGILVDLQGPKLRLGTF
VGDAAVLENGQTFVLDSDPTPGDTDRVFLPHPEILSALEPSHGILIDDGKLRLIVTEVSEGRAVTRVEVGGRISNRKGVS
LPHTVLPVPAMTEKDRGDLEAGLAAGADWIAVSFVQRPEDVAEVKKVAAGRALVMAKIEKPQALTRLDEIIEISDGIMVA
RGDLGVEMPLEQVPGVQKRITRVARRLGKPVVVATQMLESMITSPVPTRAEVSDVATAVYEGADAVMLSAESAAGDFPVE
AIGTMNRIAEQVERDALYWSILMAQRSEPEPTASDAIAAAAHQIVEALSLRSIMAWTHSGSTVLRLARARPNASVIALTP
KRETARRLTMAWGVHPIVTKDASDVDDMAFRAAKFAVRERFAEIGDRVIIVAGVPFGIPGATNMVRIAFVTREHAERA

Sequences:

>Translated_478_residues
MKRSRRTKIVATLGPASDTPEMIEKLFHAGADVFRINMSHLAREKLPERIEVIRTIEREAKRPIGILVDLQGPKLRLGTF
VGDAAVLENGQTFVLDSDPTPGDTDRVFLPHPEILSALEPSHGILIDDGKLRLIVTEVSEGRAVTRVEVGGRISNRKGVS
LPHTVLPVPAMTEKDRGDLEAGLAAGADWIAVSFVQRPEDVAEVKKVAAGRALVMAKIEKPQALTRLDEIIEISDGIMVA
RGDLGVEMPLEQVPGVQKRITRVARRLGKPVVVATQMLESMITSPVPTRAEVSDVATAVYEGADAVMLSAESAAGDFPVE
AIGTMNRIAEQVERDALYWSILMAQRSEPEPTASDAIAAAAHQIVEALSLRSIMAWTHSGSTVLRLARARPNASVIALTP
KRETARRLTMAWGVHPIVTKDASDVDDMAFRAAKFAVRERFAEIGDRVIIVAGVPFGIPGATNMVRIAFVTREHAERA
>Mature_478_residues
MKRSRRTKIVATLGPASDTPEMIEKLFHAGADVFRINMSHLAREKLPERIEVIRTIEREAKRPIGILVDLQGPKLRLGTF
VGDAAVLENGQTFVLDSDPTPGDTDRVFLPHPEILSALEPSHGILIDDGKLRLIVTEVSEGRAVTRVEVGGRISNRKGVS
LPHTVLPVPAMTEKDRGDLEAGLAAGADWIAVSFVQRPEDVAEVKKVAAGRALVMAKIEKPQALTRLDEIIEISDGIMVA
RGDLGVEMPLEQVPGVQKRITRVARRLGKPVVVATQMLESMITSPVPTRAEVSDVATAVYEGADAVMLSAESAAGDFPVE
AIGTMNRIAEQVERDALYWSILMAQRSEPEPTASDAIAAAAHQIVEALSLRSIMAWTHSGSTVLRLARARPNASVIALTP
KRETARRLTMAWGVHPIVTKDASDVDDMAFRAAKFAVRERFAEIGDRVIIVAGVPFGIPGATNMVRIAFVTREHAERA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=38.1048387096774, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=38.4146341463415, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=37.9098360655738, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=37.4233128834356, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=37.4233128834356, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=36.1111111111111, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=36.1111111111111, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=31.5126050420168, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=31.5126050420168, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.3738317757009, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1787965, Length=461, Percent_Identity=36.2255965292842, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=38.4615384615385, Blast_Score=279, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=34.4262295081967, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=35.8921161825726, Blast_Score=261, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=35.8921161825726, Blast_Score=261, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=35.8921161825726, Blast_Score=261, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=35.8921161825726, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=33.8809034907598, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=33.469387755102, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=35.3783231083845, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=35.3783231083845, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=34.8039215686275, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=31.6973415132924, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24646914, Length=260, Percent_Identity=37.6923076923077, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): KPYK_METEA (O05118)

Other databases:

- EMBL:   U87316
- EMBL:   CP001510
- RefSeq:   YP_002963970.1
- GeneID:   7992827
- ProtClustDB:   CLSK2331328
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 51802; Mature: 51802

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 35-35 BINDING 242-242 BINDING 243-243 BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSRRTKIVATLGPASDTPEMIEKLFHAGADVFRINMSHLAREKLPERIEVIRTIEREA
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
KRPIGILVDLQGPKLRLGTFVGDAAVLENGQTFVLDSDPTPGDTDRVFLPHPEILSALEP
CCCEEEEEECCCCCEEEEEEECCHHHHCCCCEEEECCCCCCCCCCEEECCCHHHHHHCCC
SHGILIDDGKLRLIVTEVSEGRAVTRVEVGGRISNRKGVSLPHTVLPVPAMTEKDRGDLE
CCCEEEECCCEEEEEEECCCCCEEEEEEECCEECCCCCCCCCCCEECCCCCCCCCCCCHH
AGLAAGADWIAVSFVQRPEDVAEVKKVAAGRALVMAKIEKPQALTRLDEIIEISDGIMVA
HHHHCCCHHEEHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEE
RGDLGVEMPLEQVPGVQKRITRVARRLGKPVVVATQMLESMITSPVPTRAEVSDVATAVY
ECCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EGADAVMLSAESAAGDFPVEAIGTMNRIAEQVERDALYWSILMAQRSEPEPTASDAIAAA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AHQIVEALSLRSIMAWTHSGSTVLRLARARPNASVIALTPKRETARRLTMAWGVHPIVTK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEC
DASDVDDMAFRAAKFAVRERFAEIGDRVIIVAGVPFGIPGATNMVRIAFVTREHAERA
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEEEEHHHHCCC
>Mature Secondary Structure
MKRSRRTKIVATLGPASDTPEMIEKLFHAGADVFRINMSHLAREKLPERIEVIRTIEREA
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
KRPIGILVDLQGPKLRLGTFVGDAAVLENGQTFVLDSDPTPGDTDRVFLPHPEILSALEP
CCCEEEEEECCCCCEEEEEEECCHHHHCCCCEEEECCCCCCCCCCEEECCCHHHHHHCCC
SHGILIDDGKLRLIVTEVSEGRAVTRVEVGGRISNRKGVSLPHTVLPVPAMTEKDRGDLE
CCCEEEECCCEEEEEEECCCCCEEEEEEECCEECCCCCCCCCCCEECCCCCCCCCCCCHH
AGLAAGADWIAVSFVQRPEDVAEVKKVAAGRALVMAKIEKPQALTRLDEIIEISDGIMVA
HHHHCCCHHEEHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEE
RGDLGVEMPLEQVPGVQKRITRVARRLGKPVVVATQMLESMITSPVPTRAEVSDVATAVY
ECCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EGADAVMLSAESAAGDFPVEAIGTMNRIAEQVERDALYWSILMAQRSEPEPTASDAIAAA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AHQIVEALSLRSIMAWTHSGSTVLRLARARPNASVIALTPKRETARRLTMAWGVHPIVTK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEC
DASDVDDMAFRAAKFAVRERFAEIGDRVIIVAGVPFGIPGATNMVRIAFVTREHAERA
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA