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The map label for this gene is mnmG

Identifier: 218711034

GI number: 218711034

Start: 3297294

End: 3299189

Strand: Reverse

Name: mnmG

Synonym: VS_3163

Alternate gene names: 218711034

Gene position: 3299189-3297294 (Counterclockwise)

Preceding gene: NA

Following gene: 218711033

Centisome position: 100.0

GC content: 46.68

Gene sequence:

>1896_bases
ATGCTTTATCACGAAAAATTTGACGTCATCGTTGTTGGTGGTGGCCATGCAGGAACGGAAGCCGCACTCGCATCTGCACG
TACTGGTCAAAGTACGTTATTACTAACTCATAATATCGATACGTTGGGACAGATGTCTTGTAACCCAGCCATTGGCGGGA
TCGGTAAGGGCCACTTGGTAAAAGAGGTCGATGCTATGGGTGGATTAATGGCGCAAGCTATTGATCATGCAGGTATTCAG
TTCAGAACATTAAACGCATCAAAAGGCCCTGCGGTACGTGCAACTCGTGCACAAGCTGACCGCGCACTTTACAAAGCCTT
TGTCCGTAATGTTCTTGAAAACACACCAAACCTAACTCTGTTCCAACAGTCGGTTGATGACTTGATCGTTGAACAAGATC
AAGTGGTGGGTGTGGTTACGCAAATGGGCCTCAAATTCCGCGCTAATGCTGTGGTCTTAACGGTAGGCACATTCCTAGGC
GGTAAGATTCATATTGGTATGGAAAGTTCATCGGGTGGACGTGCGGGTGATCCACCATCGATCGCGCTCGCTGACCGTCT
TCGTGATCTTCCATTTCGTGTTGATCGCCTGAAAACGGGTACACCTCCTCGCATCGATGCACGCAGCGTTGATTTTTCTG
AGCTTGAGGTTCAACACGGCGATAATCCGACTCCGGTTTTCTCATTCATGGGGAACCGAGCTCAGCAGCCGCGTCAGATT
CCATGTTACATCACGCACACTAACGAAAATACGCATGACGTTATTCGTGCCAACCTCGATCGCAGCCCGATGTACGCTGG
TGTGATTGAAGGTATTGGCCCTCGTTACTGTCCGTCGATTGAAGACAAAGTGATGCGCTTTGCTGATAAAAACAGTCACC
AAATTTTCATTGAGCCTGAAGGCCTAACGACGCACGAGTTATACCCGAATGGTATTTCAACCAGTTTACCGTTTGATGTT
CAGGTAAAAATCGTTCACTCAATGAAAGGTTTTGAGAACGCACACATCGTCCGTCCTGGCTACGCGATTGAGTACGACTT
CTTTGACCCTCGCGATCTTAAACTGACTTACGAGACCAAGTTTATTAAGGGGTTGTTCTTTGCGGGGCAAATCAATGGCA
CCACTGGCTACGAAGAAGCTGCAGCACAAGGCTTAATGGCCGGTTTGAATGCGAGTTTGTTTACGCAAGGCAAAGAGGGC
TGGAGCCCACGCCGCGATCAAGCTTACATGGGTGTACTTATCGATGACTTGTCGACGATGGGTACTAAAGAACCGTACCG
AATGTTTACATCTCGCGCGGAGTACCGCTTGTTGCTTCGTGAAGACAACGCTGATATCCGTTTGACTGAAAAGTCACGTG
AACTTGGCTTGGTCGACGATGCTCGTTGGACGCGATTCAACGAGAAGATGGAAAACATGGAGAAAGAGCGTCAACGTCTT
AAAGAAACATGGATTAATCCAAAATCTGAAGATATTGATCAATTGAACCAGATCCTGAAAACACCAATGTCTCGTGAAGC
GAGTGGTGAAGATCTTCTTCGTCGTCCTGAAATGACTTATTCACAGCTAACGTCGTTGGATCGTTTTGGTCCTGCACTGG
AAGATCAACAAGCGTCTGAGCAAGTTGAGATCCAAGTGAAGTACGATGGCTATATCCAACGTCAACAAGATGAAATTGAA
AAATCACTGCGTCATGAAAACACCAAACTGCCGGCTGACATCGATTACAGTAAGGTAAAAGGGCTTTCTAACGAAGTGGT
TCTTAAACTAACTACCGCTAAACCAGACTCAATTGGTATTGCATCGCGAATCTCAGGTATCACACCTGCTGCAATTTCAA
TTCTGTTGGTTTACTTGAAAAAACACGGCCTGTTGAAAAAAGGTGAGGAAGCATAA

Upstream 100 bases:

>100_bases
TTTCGAAAAATTCCAAAAACGTTTTCTCAAGCTATGAAAACAGGTAGAATATCGCTCCTTTTTATCAATCTAGAAACGTT
TGAGTACCTGAGGTCGGTTC

Downstream 100 bases:

>100_bases
TGAGCGCATTACGCGAAAAACTGGATCACCTGATTGGCCAGACTGAACTTGAAGTATCTGAAAAGCAACGCAGCCAATTG
GTTGGCTACGTTGAGTTACT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MLYHEKFDVIVVGGGHAGTEAALASARTGQSTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAQAIDHAGIQ
FRTLNASKGPAVRATRAQADRALYKAFVRNVLENTPNLTLFQQSVDDLIVEQDQVVGVVTQMGLKFRANAVVLTVGTFLG
GKIHIGMESSSGGRAGDPPSIALADRLRDLPFRVDRLKTGTPPRIDARSVDFSELEVQHGDNPTPVFSFMGNRAQQPRQI
PCYITHTNENTHDVIRANLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKNSHQIFIEPEGLTTHELYPNGISTSLPFDV
QVKIVHSMKGFENAHIVRPGYAIEYDFFDPRDLKLTYETKFIKGLFFAGQINGTTGYEEAAAQGLMAGLNASLFTQGKEG
WSPRRDQAYMGVLIDDLSTMGTKEPYRMFTSRAEYRLLLREDNADIRLTEKSRELGLVDDARWTRFNEKMENMEKERQRL
KETWINPKSEDIDQLNQILKTPMSREASGEDLLRRPEMTYSQLTSLDRFGPALEDQQASEQVEIQVKYDGYIQRQQDEIE
KSLRHENTKLPADIDYSKVKGLSNEVVLKLTTAKPDSIGIASRISGITPAAISILLVYLKKHGLLKKGEEA

Sequences:

>Translated_631_residues
MLYHEKFDVIVVGGGHAGTEAALASARTGQSTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAQAIDHAGIQ
FRTLNASKGPAVRATRAQADRALYKAFVRNVLENTPNLTLFQQSVDDLIVEQDQVVGVVTQMGLKFRANAVVLTVGTFLG
GKIHIGMESSSGGRAGDPPSIALADRLRDLPFRVDRLKTGTPPRIDARSVDFSELEVQHGDNPTPVFSFMGNRAQQPRQI
PCYITHTNENTHDVIRANLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKNSHQIFIEPEGLTTHELYPNGISTSLPFDV
QVKIVHSMKGFENAHIVRPGYAIEYDFFDPRDLKLTYETKFIKGLFFAGQINGTTGYEEAAAQGLMAGLNASLFTQGKEG
WSPRRDQAYMGVLIDDLSTMGTKEPYRMFTSRAEYRLLLREDNADIRLTEKSRELGLVDDARWTRFNEKMENMEKERQRL
KETWINPKSEDIDQLNQILKTPMSREASGEDLLRRPEMTYSQLTSLDRFGPALEDQQASEQVEIQVKYDGYIQRQQDEIE
KSLRHENTKLPADIDYSKVKGLSNEVVLKLTTAKPDSIGIASRISGITPAAISILLVYLKKHGLLKKGEEA
>Mature_631_residues
MLYHEKFDVIVVGGGHAGTEAALASARTGQSTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDAMGGLMAQAIDHAGIQ
FRTLNASKGPAVRATRAQADRALYKAFVRNVLENTPNLTLFQQSVDDLIVEQDQVVGVVTQMGLKFRANAVVLTVGTFLG
GKIHIGMESSSGGRAGDPPSIALADRLRDLPFRVDRLKTGTPPRIDARSVDFSELEVQHGDNPTPVFSFMGNRAQQPRQI
PCYITHTNENTHDVIRANLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKNSHQIFIEPEGLTTHELYPNGISTSLPFDV
QVKIVHSMKGFENAHIVRPGYAIEYDFFDPRDLKLTYETKFIKGLFFAGQINGTTGYEEAAAQGLMAGLNASLFTQGKEG
WSPRRDQAYMGVLIDDLSTMGTKEPYRMFTSRAEYRLLLREDNADIRLTEKSRELGLVDDARWTRFNEKMENMEKERQRL
KETWINPKSEDIDQLNQILKTPMSREASGEDLLRRPEMTYSQLTSLDRFGPALEDQQASEQVEIQVKYDGYIQRQQDEIE
KSLRHENTKLPADIDYSKVKGLSNEVVLKLTTAKPDSIGIASRISGITPAAISILLVYLKKHGLLKKGEEA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=631, Percent_Identity=45.4833597464342, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=43.75, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=42.7719821162444, Blast_Score=484, Evalue=1e-136,
Organism=Escherichia coli, GI2367273, Length=627, Percent_Identity=77.6714513556619, Blast_Score=1030, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=628, Percent_Identity=40.7643312101911, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321202, Length=623, Percent_Identity=43.8202247191011, Blast_Score=493, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=47.4842767295598, Blast_Score=537, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_VIBSL (B7VN07)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418655.1
- ProteinModelPortal:   B7VN07
- GeneID:   7162601
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3163
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70187; Mature: 70187

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 125-125 BINDING 180-180 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYHEKFDVIVVGGGHAGTEAALASARTGQSTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCEECEEEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEVDAMGGLMAQAIDHAGIQFRTLNASKGPAVRATRAQADRALYKAFVRNVLENTPNLTL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
FQQSVDDLIVEQDQVVGVVTQMGLKFRANAVVLTVGTFLGGKIHIGMESSSGGRAGDPPS
EHHHHHHHHHCCHHHHHHHHHCCCEEECCEEEEEEECCCCCEEEEEEECCCCCCCCCCCC
IALADRLRDLPFRVDRLKTGTPPRIDARSVDFSELEVQHGDNPTPVFSFMGNRAQQPRQI
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCCHHHHHHCCCCCCCCCC
PCYITHTNENTHDVIRANLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKNSHQIFIEPE
CEEEEECCCCCHHHHEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLTTHELYPNGISTSLPFDVQVKIVHSMKGFENAHIVRPGYAIEYDFFDPRDLKLTYETK
CCEEEEECCCCCCCCCCCEEEEEEEEHHCCCCCCEEECCCEEEEECCCCCCCCEEEEEHH
FIKGLFFAGQINGTTGYEEAAAQGLMAGLNASLFTQGKEGWSPRRDQAYMGVLIDDLSTM
EEEEEEEEEEECCCCCHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLLLREDNADIRLTEKSRELGLVDDARWTRFNEKMENMEKERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KETWINPKSEDIDQLNQILKTPMSREASGEDLLRRPEMTYSQLTSLDRFGPALEDQQASE
HHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
QVEIQVKYDGYIQRQQDEIEKSLRHENTKLPADIDYSKVKGLSNEVVLKLTTAKPDSIGI
EEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCH
ASRISGITPAAISILLVYLKKHGLLKKGEEA
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLYHEKFDVIVVGGGHAGTEAALASARTGQSTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCEECEEEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEVDAMGGLMAQAIDHAGIQFRTLNASKGPAVRATRAQADRALYKAFVRNVLENTPNLTL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
FQQSVDDLIVEQDQVVGVVTQMGLKFRANAVVLTVGTFLGGKIHIGMESSSGGRAGDPPS
EHHHHHHHHHCCHHHHHHHHHCCCEEECCEEEEEEECCCCCEEEEEEECCCCCCCCCCCC
IALADRLRDLPFRVDRLKTGTPPRIDARSVDFSELEVQHGDNPTPVFSFMGNRAQQPRQI
HHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCCHHHHHHCCCCCCCCCC
PCYITHTNENTHDVIRANLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADKNSHQIFIEPE
CEEEEECCCCCHHHHEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLTTHELYPNGISTSLPFDVQVKIVHSMKGFENAHIVRPGYAIEYDFFDPRDLKLTYETK
CCEEEEECCCCCCCCCCCEEEEEEEEHHCCCCCCEEECCCEEEEECCCCCCCCEEEEEHH
FIKGLFFAGQINGTTGYEEAAAQGLMAGLNASLFTQGKEGWSPRRDQAYMGVLIDDLSTM
EEEEEEEEEEECCCCCHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLLLREDNADIRLTEKSRELGLVDDARWTRFNEKMENMEKERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KETWINPKSEDIDQLNQILKTPMSREASGEDLLRRPEMTYSQLTSLDRFGPALEDQQASE
HHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
QVEIQVKYDGYIQRQQDEIEKSLRHENTKLPADIDYSKVKGLSNEVVLKLTTAKPDSIGI
EEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCH
ASRISGITPAAISILLVYLKKHGLLKKGEEA
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA