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The map label for this gene is gidB

Identifier: 218711033

GI number: 218711033

Start: 3296662

End: 3297294

Strand: Reverse

Name: gidB

Synonym: VS_3162

Alternate gene names: 218711033

Gene position: 3297294-3296662 (Counterclockwise)

Preceding gene: 218711034

Following gene: 218711032

Centisome position: 99.94

GC content: 44.55

Gene sequence:

>633_bases
ATGAGCGCATTACGCGAAAAACTGGATCACCTGATTGGCCAGACTGAACTTGAAGTATCTGAAAAGCAACGCAGCCAATT
GGTTGGCTACGTTGAGTTACTAAACAAGTGGAACAAAGCATACAATCTGACGTCAGTTCGTGATCCGCAAGAAATGATGG
TGAAACATATCTTAGATAGCATCATTGTTAGCACTCACTTACAAGGCAAGCGCTTTATTGACGTTGGCACAGGCCCTGGT
CTTCCTGGGATTCCGCTCTCAATCATGAATCCTGACTGCGAGTTTTACTTGCTAGACAGCTTGGGTAAGCGTATTCGTTT
TATCAAGCAAGTGATTCATGAGCTGGGTATCGACAACGTGGTGCCGATTCAAAGTCGCGTTGAAGAATTTCAACCAGAAG
AAAAGTTTGATGCCGTGCTCAGTCGCGCATTTGCATCAATGACAGACATGGTAGAGTGGTGTCATCATTTACCTAAAGAG
CAATCCGGTGTATTTTTGGCTCTTAAGGGACAACATCCTAGAGACGAAATCGACCTGTTACCTGAATGGTGTTCAGTGAC
AGACATTAAAGCTTTGCAAGTGCCAGAGCTTGATGGAGAGCGTCATCTAGTTACTTTATCGCGTCAGGGATAA

Upstream 100 bases:

>100_bases
GTATTGCATCGCGAATCTCAGGTATCACACCTGCTGCAATTTCAATTCTGTTGGTTTACTTGAAAAAACACGGCCTGTTG
AAAAAAGGTGAGGAAGCATA

Downstream 100 bases:

>100_bases
TCAGCGAGGCCATAGTGGGTAAAATCGTAGCAATTGCCAACCAGAAGGGTGGCGTAGGAAAAACAACAACTTGCATTAAC
TTGGCAGCATCAATGGCGGC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSALREKLDHLIGQTELEVSEKQRSQLVGYVELLNKWNKAYNLTSVRDPQEMMVKHILDSIIVSTHLQGKRFIDVGTGPG
LPGIPLSIMNPDCEFYLLDSLGKRIRFIKQVIHELGIDNVVPIQSRVEEFQPEEKFDAVLSRAFASMTDMVEWCHHLPKE
QSGVFLALKGQHPRDEIDLLPEWCSVTDIKALQVPELDGERHLVTLSRQG

Sequences:

>Translated_210_residues
MSALREKLDHLIGQTELEVSEKQRSQLVGYVELLNKWNKAYNLTSVRDPQEMMVKHILDSIIVSTHLQGKRFIDVGTGPG
LPGIPLSIMNPDCEFYLLDSLGKRIRFIKQVIHELGIDNVVPIQSRVEEFQPEEKFDAVLSRAFASMTDMVEWCHHLPKE
QSGVFLALKGQHPRDEIDLLPEWCSVTDIKALQVPELDGERHLVTLSRQG
>Mature_209_residues
SALREKLDHLIGQTELEVSEKQRSQLVGYVELLNKWNKAYNLTSVRDPQEMMVKHILDSIIVSTHLQGKRFIDVGTGPGL
PGIPLSIMNPDCEFYLLDSLGKRIRFIKQVIHELGIDNVVPIQSRVEEFQPEEKFDAVLSRAFASMTDMVEWCHHLPKEQ
SGVFLALKGQHPRDEIDLLPEWCSVTDIKALQVPELDGERHLVTLSRQG

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=200, Percent_Identity=64.5, Blast_Score=271, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_VIBSL (B7VN06)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418654.1
- ProteinModelPortal:   B7VN06
- SMR:   B7VN06
- GeneID:   7162600
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3162
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23921; Mature: 23790

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALREKLDHLIGQTELEVSEKQRSQLVGYVELLNKWNKAYNLTSVRDPQEMMVKHILDS
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IIVSTHLQGKRFIDVGTGPGLPGIPLSIMNPDCEFYLLDSLGKRIRFIKQVIHELGIDNV
HHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCC
VPIQSRVEEFQPEEKFDAVLSRAFASMTDMVEWCHHLPKEQSGVFLALKGQHPRDEIDLL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHC
PEWCSVTDIKALQVPELDGERHLVTLSRQG
HHHCCCCCCCEEECCCCCCCCEEEEEECCC
>Mature Secondary Structure 
SALREKLDHLIGQTELEVSEKQRSQLVGYVELLNKWNKAYNLTSVRDPQEMMVKHILDS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
IIVSTHLQGKRFIDVGTGPGLPGIPLSIMNPDCEFYLLDSLGKRIRFIKQVIHELGIDNV
HHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCC
VPIQSRVEEFQPEEKFDAVLSRAFASMTDMVEWCHHLPKEQSGVFLALKGQHPRDEIDLL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHC
PEWCSVTDIKALQVPELDGERHLVTLSRQG
HHHCCCCCCCEEECCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA