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The map label for this gene is rsmB [H]

Identifier: 218711000

GI number: 218711000

Start: 3263445

End: 3264725

Strand: Direct

Name: rsmB [H]

Synonym: VS_3128

Alternate gene names: 218711000

Gene position: 3263445-3264725 (Clockwise)

Preceding gene: 218710999

Following gene: 218711001

Centisome position: 98.91

GC content: 48.63

Gene sequence:

>1281_bases
ATGAATGTTCGCGCTGCTGCTGCAAATGTCCTATTCCAAGTTGTCGATAAAGGCCACTCTCTTTCACACGCTCTCCCTGC
GGCTCAAAAAACGATCCGTCCGCGAGACCATGCTCTACTGCAAGAGATTTGCTACGGCGCACTTCGTTACCTGCCTCGCT
TGGAGTCAATCGCTAACGAACTGATGGAAAACTCGCTTAAAGGTAAAAAGCGCGTATTCCACCACCTGATCTTAGTAGGC
ATTTACCAGTTGAGCTTTATGCGCATTCCTTCGCATGCTGCCGTTGCTGAAACAGTTGAAGCAACCAAAACACTGCGCGG
CCCAAGCCTGAGTGGTTTGATCAACGCAGTGCTTCGCAGCTACCTGCGCGATCAAGAAGAGCTAGATGAGAAAGCCGTTA
GCCACAATGCGGGCAAATACAGCCATCCAAGCTGGATCCTAAAAATGCTTCAAGAAAGCTACCCAGATCAGTGGGAGCAA
CTAGTTGAAGCAAACAACAGCAAGGCACCAATGTGGCTACGCGTAAACCGCCAACACCACACTCGTGACGAGTATGTTGA
ACTGCTTAAAAACGAAAACATTGAATACACACTGCACCCAGAAGCGGCTGATGCCATAAAATTAGCGTCACCTTGTGATG
TTACTTTGCTTCCTGGTTTCGACCGAGGCTGGGTATCAGTACAAGACGCTGCAGCTCAGCTTTCCGTTGATTTCCTAACA
CCAAAAGATGGTGAGTTAATCCTAGACTGCTGCGCAGCACCTGGCGGTAAGACTGCACACATTCTTGAACATACTCAAGA
CACTGAAGTGGTTGCAATCGACAGTGATATTAAACGCCTAGACCGTGTTTACGATAACCTTGAACGCCTACAACTACGTG
CCGACGTAATCTGTGGTGATGCTCGCTACCCTGAAGAATGGTGGACTGGCAGTCAGTTTGACCGCATCCTACTTGATGCT
CCATGTTCAGCAACCGGTGTAATTCGCCGTCACCCTGACATTAAGTGGCTACGCCGTGCATCTGATATTGATGCACTAGC
AGAGCTACAAAGTGAGATTTTGGATGCAATGTGGCGCCAGCTAAAAGAAGGCGGCACCATGGTTTATGCGACATGTTCTA
TCACGCCGCAAGAAAACGTGCTGCAAGTGAAAGCATTCTTAGAGCGTACCGAGAACGCAACGCTGGTTGGGTCTGATATC
GAAAAACCGGGTCGTCAAATACTGCCTGGTGAAGAAGATATGGATGGCTTCTACTACGCAGTTCTAGTAAAACAGGCATA
A

Upstream 100 bases:

>100_bases
TAGGCACTTAAAAACGTGTAGCTTGTAAAAACACAGTTTAGAAAACCTTTACGAGGGCAGAGATGCCCTCATGTATTCAA
ATAAATATTCGGTACCCCTC

Downstream 100 bases:

>100_bases
CAACTAACAGCGGCTAAGAATTTATTTCTTAGCCGCTGTTTCTTTCTAGTGCATTATCAAAGCGAAAAGAGATCACTAGA
ATTACAACGGCAAAAAATAA

Product: ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNVRAAAANVLFQVVDKGHSLSHALPAAQKTIRPRDHALLQEICYGALRYLPRLESIANELMENSLKGKKRVFHHLILVG
IYQLSFMRIPSHAAVAETVEATKTLRGPSLSGLINAVLRSYLRDQEELDEKAVSHNAGKYSHPSWILKMLQESYPDQWEQ
LVEANNSKAPMWLRVNRQHHTRDEYVELLKNENIEYTLHPEAADAIKLASPCDVTLLPGFDRGWVSVQDAAAQLSVDFLT
PKDGELILDCCAAPGGKTAHILEHTQDTEVVAIDSDIKRLDRVYDNLERLQLRADVICGDARYPEEWWTGSQFDRILLDA
PCSATGVIRRHPDIKWLRRASDIDALAELQSEILDAMWRQLKEGGTMVYATCSITPQENVLQVKAFLERTENATLVGSDI
EKPGRQILPGEEDMDGFYYAVLVKQA

Sequences:

>Translated_426_residues
MNVRAAAANVLFQVVDKGHSLSHALPAAQKTIRPRDHALLQEICYGALRYLPRLESIANELMENSLKGKKRVFHHLILVG
IYQLSFMRIPSHAAVAETVEATKTLRGPSLSGLINAVLRSYLRDQEELDEKAVSHNAGKYSHPSWILKMLQESYPDQWEQ
LVEANNSKAPMWLRVNRQHHTRDEYVELLKNENIEYTLHPEAADAIKLASPCDVTLLPGFDRGWVSVQDAAAQLSVDFLT
PKDGELILDCCAAPGGKTAHILEHTQDTEVVAIDSDIKRLDRVYDNLERLQLRADVICGDARYPEEWWTGSQFDRILLDA
PCSATGVIRRHPDIKWLRRASDIDALAELQSEILDAMWRQLKEGGTMVYATCSITPQENVLQVKAFLERTENATLVGSDI
EKPGRQILPGEEDMDGFYYAVLVKQA
>Mature_426_residues
MNVRAAAANVLFQVVDKGHSLSHALPAAQKTIRPRDHALLQEICYGALRYLPRLESIANELMENSLKGKKRVFHHLILVG
IYQLSFMRIPSHAAVAETVEATKTLRGPSLSGLINAVLRSYLRDQEELDEKAVSHNAGKYSHPSWILKMLQESYPDQWEQ
LVEANNSKAPMWLRVNRQHHTRDEYVELLKNENIEYTLHPEAADAIKLASPCDVTLLPGFDRGWVSVQDAAAQLSVDFLT
PKDGELILDCCAAPGGKTAHILEHTQDTEVVAIDSDIKRLDRVYDNLERLQLRADVICGDARYPEEWWTGSQFDRILLDA
PCSATGVIRRHPDIKWLRRASDIDALAELQSEILDAMWRQLKEGGTMVYATCSITPQENVLQVKAFLERTENATLVGSDI
EKPGRQILPGEEDMDGFYYAVLVKQA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=346, Percent_Identity=32.0809248554913, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI76150623, Length=346, Percent_Identity=32.0809248554913, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI32698918, Length=196, Percent_Identity=34.6938775510204, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI301336155, Length=217, Percent_Identity=32.258064516129, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI39995082, Length=217, Percent_Identity=32.258064516129, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI270288818, Length=305, Percent_Identity=25.5737704918033, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI270288816, Length=305, Percent_Identity=25.5737704918033, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI8922322, Length=305, Percent_Identity=25.5737704918033, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI23199998, Length=305, Percent_Identity=25.5737704918033, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI40316918, Length=184, Percent_Identity=30.4347826086957, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=425, Percent_Identity=56, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI87081985, Length=217, Percent_Identity=29.9539170506912, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536757, Length=265, Percent_Identity=31.3207547169811, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25143471, Length=171, Percent_Identity=37.4269005847953, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324268, Length=310, Percent_Identity=29.6774193548387, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319447, Length=297, Percent_Identity=27.6094276094276, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI22024126, Length=314, Percent_Identity=30.2547770700637, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19920494, Length=164, Percent_Identity=32.3170731707317, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355201, Length=211, Percent_Identity=31.2796208530806, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47954; Mature: 47954

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRAAAANVLFQVVDKGHSLSHALPAAQKTIRPRDHALLQEICYGALRYLPRLESIANE
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMENSLKGKKRVFHHLILVGIYQLSFMRIPSHAAVAETVEATKTLRGPSLSGLINAVLRS
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YLRDQEELDEKAVSHNAGKYSHPSWILKMLQESYPDQWEQLVEANNSKAPMWLRVNRQHH
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCC
TRDEYVELLKNENIEYTLHPEAADAIKLASPCDVTLLPGFDRGWVSVQDAAAQLSVDFLT
CHHHHHHHHHCCCCEEEECCCCHHHHEECCCCCEEEECCCCCCCEEHHHHHHHEEEEEEC
PKDGELILDCCAAPGGKTAHILEHTQDTEVVAIDSDIKRLDRVYDNLERLQLRADVICGD
CCCCCEEEEECCCCCCCEEHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEECC
ARYPEEWWTGSQFDRILLDAPCSATGVIRRHPDIKWLRRASDIDALAELQSEILDAMWRQ
CCCCHHHCCCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
LKEGGTMVYATCSITPQENVLQVKAFLERTENATLVGSDIEKPGRQILPGEEDMDGFYYA
HHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEE
VLVKQA
EEEECC
>Mature Secondary Structure
MNVRAAAANVLFQVVDKGHSLSHALPAAQKTIRPRDHALLQEICYGALRYLPRLESIANE
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMENSLKGKKRVFHHLILVGIYQLSFMRIPSHAAVAETVEATKTLRGPSLSGLINAVLRS
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YLRDQEELDEKAVSHNAGKYSHPSWILKMLQESYPDQWEQLVEANNSKAPMWLRVNRQHH
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCC
TRDEYVELLKNENIEYTLHPEAADAIKLASPCDVTLLPGFDRGWVSVQDAAAQLSVDFLT
CHHHHHHHHHCCCCEEEECCCCHHHHEECCCCCEEEECCCCCCCEEHHHHHHHEEEEEEC
PKDGELILDCCAAPGGKTAHILEHTQDTEVVAIDSDIKRLDRVYDNLERLQLRADVICGD
CCCCCEEEEECCCCCCCEEHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEECC
ARYPEEWWTGSQFDRILLDAPCSATGVIRRHPDIKWLRRASDIDALAELQSEILDAMWRQ
CCCCHHHCCCCCCCCEEEECCCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
LKEGGTMVYATCSITPQENVLQVKAFLERTENATLVGSDIEKPGRQILPGEEDMDGFYYA
HHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEE
VLVKQA
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]