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The map label for this gene is fmt

Identifier: 218710999

GI number: 218710999

Start: 3262360

End: 3263325

Strand: Direct

Name: fmt

Synonym: VS_3127

Alternate gene names: 218710999

Gene position: 3262360-3263325 (Clockwise)

Preceding gene: 218710998

Following gene: 218711000

Centisome position: 98.88

GC content: 46.38

Gene sequence:

>966_bases
TTGAGTCAATCTTTAAGAATTGTCTTCGCAGGTACTCCGGATTTCGCCGCCCGTCATTTGGCGGCGTTGTTGTCTTCGGA
GCATGAAGTTATTGCTGTTTACACACAGCCAGATCGTCCAGCAGGCCGCGGTAAAAAACTGACTGCGAGCCCAGTAAAAA
ACATCGCACTTGAAAACAATATTCCGGTTTACCAACCAGAAAACTTCAAGTCAGATGAAGCTAAGCAAGAGCTAGCAAAT
TTGAATGCTGACATCATGGTTGTTGTCGCTTACGGCTTGCTTCTTCCACAAGCTGTATTAGATACGCCTCGCTTGGGTTG
TATTAACGTGCATGGTTCTATCCTACCGCGCTGGCGTGGTGCTGCTCCAATCCAACGCTCTATCTGGGCGGGCGATAAAG
AGACTGGCGTAACGATCATGCAAATGGATATCGGCCTAGATACCGGTGATATGCTCAGCATCGCAACGCTACCAATCGAA
TCTACAGATACTAGCGCGTCAATGTATGAGAAGTTGGCAGGCCTTGGTCCTGATGCTCTTGTTGAGTGTTTAGCAGACAT
CGCTTCTGGTAAGGCTGTTGCTGAGAAGCAAGACGACGAACTTGCTAACTACGCGAAGAAGCTGAGCAAAGAAGAAGCTA
AAATTGATTGGAATGACAGTGCAGAACACATCGAACGTTGTGTGCGCGCTTTCAACCCGTGGCCAATGAGTCACTTTGCG
ATTGTTGATAGCAGCTCTAATGATGAAAAAAGCATTAAAGTTTGGCAGACGCGTGTTGATGAAGAATCGACATCTGCGCC
AGCTGGCTCAATCATCAAAGCAGATAAGACTGGTATTTATGTTGCGACTGGCGACAAAGTACTAGTATTGGAACAGCTAC
AAGTTCCAGGCAAAAAAGCTATGTCAGTTCAAGACATCTTGAACTCACGTGCAAGCTGGTTTGAAGTTGGTACTCAACTC
TCTTAA

Upstream 100 bases:

>100_bases
AGCGCAAACGTATTCAAGATAAGCTAGCGAAGATTAAACGTTTCAACGAGAAACAAGGTTAATAACCGCTGCTATTACTA
AATTAAGAAGGAAGTCTACC

Downstream 100 bases:

>100_bases
CCGCTTTAAAGCTACTCAATAGGCACTTAAAAACGTGTAGCTTGTAAAAACACAGTTTAGAAAACCTTTACGAGGGCAGA
GATGCCCTCATGTATTCAAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSQSLRIVFAGTPDFAARHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKNIALENNIPVYQPENFKSDEAKQELAN
LNADIMVVVAYGLLLPQAVLDTPRLGCINVHGSILPRWRGAAPIQRSIWAGDKETGVTIMQMDIGLDTGDMLSIATLPIE
STDTSASMYEKLAGLGPDALVECLADIASGKAVAEKQDDELANYAKKLSKEEAKIDWNDSAEHIERCVRAFNPWPMSHFA
IVDSSSNDEKSIKVWQTRVDEESTSAPAGSIIKADKTGIYVATGDKVLVLEQLQVPGKKAMSVQDILNSRASWFEVGTQL
S

Sequences:

>Translated_321_residues
MSQSLRIVFAGTPDFAARHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKNIALENNIPVYQPENFKSDEAKQELAN
LNADIMVVVAYGLLLPQAVLDTPRLGCINVHGSILPRWRGAAPIQRSIWAGDKETGVTIMQMDIGLDTGDMLSIATLPIE
STDTSASMYEKLAGLGPDALVECLADIASGKAVAEKQDDELANYAKKLSKEEAKIDWNDSAEHIERCVRAFNPWPMSHFA
IVDSSSNDEKSIKVWQTRVDEESTSAPAGSIIKADKTGIYVATGDKVLVLEQLQVPGKKAMSVQDILNSRASWFEVGTQL
S
>Mature_320_residues
SQSLRIVFAGTPDFAARHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKNIALENNIPVYQPENFKSDEAKQELANL
NADIMVVVAYGLLLPQAVLDTPRLGCINVHGSILPRWRGAAPIQRSIWAGDKETGVTIMQMDIGLDTGDMLSIATLPIES
TDTSASMYEKLAGLGPDALVECLADIASGKAVAEKQDDELANYAKKLSKEEAKIDWNDSAEHIERCVRAFNPWPMSHFAI
VDSSSNDEKSIKVWQTRVDEESTSAPAGSIIKADKTGIYVATGDKVLVLEQLQVPGKKAMSVQDILNSRASWFEVGTQLS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=33.8983050847458, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI238814322, Length=241, Percent_Identity=29.8755186721992, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI21614513, Length=324, Percent_Identity=26.8518518518519, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1789683, Length=320, Percent_Identity=62.1875, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1788589, Length=283, Percent_Identity=28.6219081272085, Blast_Score=120, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI133930964, Length=314, Percent_Identity=24.203821656051, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45550868, Length=309, Percent_Identity=28.4789644012945, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571984, Length=231, Percent_Identity=31.1688311688312, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585660, Length=263, Percent_Identity=30.4182509505703, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_VIBSL (B7VMX2)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418620.1
- GeneID:   7162566
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3127
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34832; Mature: 34701

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSLRIVFAGTPDFAARHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKNIALENN
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCHHHHHEECCC
IPVYQPENFKSDEAKQELANLNADIMVVVAYGLLLPQAVLDTPRLGCINVHGSILPRWRG
CCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDKETGVTIMQMDIGLDTGDMLSIATLPIESTDTSASMYEKLAGLGPDAL
CCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
VECLADIASGKAVAEKQDDELANYAKKLSKEEAKIDWNDSAEHIERCVRAFNPWPMSHFA
HHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEE
IVDSSSNDEKSIKVWQTRVDEESTSAPAGSIIKADKTGIYVATGDKVLVLEQLQVPGKKA
EEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCEEEEEEECCCCCHHH
MSVQDILNSRASWFEVGTQLS
HHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure 
SQSLRIVFAGTPDFAARHLAALLSSEHEVIAVYTQPDRPAGRGKKLTASPVKNIALENN
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEECCHHHHHEECCC
IPVYQPENFKSDEAKQELANLNADIMVVVAYGLLLPQAVLDTPRLGCINVHGSILPRWRG
CCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDKETGVTIMQMDIGLDTGDMLSIATLPIESTDTSASMYEKLAGLGPDAL
CCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
VECLADIASGKAVAEKQDDELANYAKKLSKEEAKIDWNDSAEHIERCVRAFNPWPMSHFA
HHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEE
IVDSSSNDEKSIKVWQTRVDEESTSAPAGSIIKADKTGIYVATGDKVLVLEQLQVPGKKA
EEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCEEEEEEECCCCCHHH
MSVQDILNSRASWFEVGTQLS
HHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA