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The map label for this gene is hemF

Identifier: 218710990

GI number: 218710990

Start: 3255010

End: 3255927

Strand: Reverse

Name: hemF

Synonym: VS_3118

Alternate gene names: 218710990

Gene position: 3255927-3255010 (Counterclockwise)

Preceding gene: 218710991

Following gene: 218710989

Centisome position: 98.69

GC content: 47.93

Gene sequence:

>918_bases
ATGTCAGCAATTGATAAAGAAGCAGTAAAGCAGTTTTTACTGAGCCTACAAGATTCGATTTGCCAGCAGCTTGAGCAAGC
TGATGGTACTGCACTGTTTGAAGAAGATGCATGGCAGCGTGAACCTGGCGAGCGCCTGGGTGGCGGTGGTCGAACTCGTG
TGATGACCAACGGTGCGGTATTTGAGCAAGGTGGTGTTAACTTTTCTCACGTTGCAGGTAAGGCAATGCCAGCTTCAGCA
ACCGCTCACCGCCCTGAATTAGCCGGACGTAAGTTTGAAGCTATGGGGGTGTCGTTAGTTATCCATCCTAAAAACCCATA
CATCCCAACCTCACACGCGAACGTTCGATTCTTCATAGCAGAAAAAGAAGGGGAAGACCCAATTTGGTGGTTTGGTGGTG
GTTTTGACTTAACGCCATTTTATCCTGTCGATGAAGATTGCCAATCTTGGCATCAAACCGCCAAAGATCTTTGTGCGCCA
TTTGGAGATGATGTTTATCAAGAGCACAAAGAGTGGTGTGATAAGTACTTCTATCTTCCGCATAGAGATGAAACACGTGG
TGTGGGTGGGTTGTTCTTTGATGATCTTAACGAGTGGGGCTTTGAAAAGAGCTTTGCTTATATGCAGGCGGTCGGTGAAG
GTTTCGCAGCCGCATACTTACCAATTGTAGAACGACGCAAAGAGACGCCTTATGGTGAGCGCGAGCGTGATTTCCAACTT
TATCGTCGTGGTCGCTATGTTGAATTTAACTTAGTGTATGACCGTGGCACCCTATTTGGTCTACAAAGCGGTGGTCGCAC
AGAGTCTATATTGATGTCGATGCCGCCATTGGCTCGCTGGGAATACCGCTACGAGCCACAAGCGTGTTCACCAGAAGCTC
TGCTTTATAGCGACTACCTAAAGCCTCGAGTTTGGTAG

Upstream 100 bases:

>100_bases
TTAGAAGGTCAAACGGGTGGTCGTGAGAAGCCAAGCGAAATTAGAGATGCAAAAACGTCGAAAATATTAAGACAGGGTTA
AACCCTGCAGAGGAAGAAAG

Downstream 100 bases:

>100_bases
TTCTTCCTTCCTTATAGGGATAGTGATAGGGTCATCTATATAGATGACCCTTTTTTATTTCTGTTAGGTAAGGATATGAC
ACAGCAAGTAGATCGTTATG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSAIDKEAVKQFLLSLQDSICQQLEQADGTALFEEDAWQREPGERLGGGGRTRVMTNGAVFEQGGVNFSHVAGKAMPASA
TAHRPELAGRKFEAMGVSLVIHPKNPYIPTSHANVRFFIAEKEGEDPIWWFGGGFDLTPFYPVDEDCQSWHQTAKDLCAP
FGDDVYQEHKEWCDKYFYLPHRDETRGVGGLFFDDLNEWGFEKSFAYMQAVGEGFAAAYLPIVERRKETPYGERERDFQL
YRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSMPPLARWEYRYEPQACSPEALLYSDYLKPRVW

Sequences:

>Translated_305_residues
MSAIDKEAVKQFLLSLQDSICQQLEQADGTALFEEDAWQREPGERLGGGGRTRVMTNGAVFEQGGVNFSHVAGKAMPASA
TAHRPELAGRKFEAMGVSLVIHPKNPYIPTSHANVRFFIAEKEGEDPIWWFGGGFDLTPFYPVDEDCQSWHQTAKDLCAP
FGDDVYQEHKEWCDKYFYLPHRDETRGVGGLFFDDLNEWGFEKSFAYMQAVGEGFAAAYLPIVERRKETPYGERERDFQL
YRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSMPPLARWEYRYEPQACSPEALLYSDYLKPRVW
>Mature_304_residues
SAIDKEAVKQFLLSLQDSICQQLEQADGTALFEEDAWQREPGERLGGGGRTRVMTNGAVFEQGGVNFSHVAGKAMPASAT
AHRPELAGRKFEAMGVSLVIHPKNPYIPTSHANVRFFIAEKEGEDPIWWFGGGFDLTPFYPVDEDCQSWHQTAKDLCAPF
GDDVYQEHKEWCDKYFYLPHRDETRGVGGLFFDDLNEWGFEKSFAYMQAVGEGFAAAYLPIVERRKETPYGERERDFQLY
RRGRYVEFNLVYDRGTLFGLQSGGRTESILMSMPPLARWEYRYEPQACSPEALLYSDYLKPRVW

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=308, Percent_Identity=43.8311688311688, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI1788777, Length=301, Percent_Identity=73.421926910299, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320249, Length=318, Percent_Identity=43.7106918238994, Blast_Score=251, Evalue=8e-68,
Organism=Drosophila melanogaster, GI17864388, Length=313, Percent_Identity=43.4504792332268, Blast_Score=257, Evalue=8e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_VIBSL (B7VMW3)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418611.1
- GeneID:   7162557
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3118
- HOGENOM:   HBG631180
- OMA:   HMNTRFV
- ProtClustDB:   PRK05330
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 34881; Mature: 34750

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 112-112 BINDING 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIDKEAVKQFLLSLQDSICQQLEQADGTALFEEDAWQREPGERLGGGGRTRVMTNGAV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHCCCCCEEEEECCCE
FEQGGVNFSHVAGKAMPASATAHRPELAGRKFEAMGVSLVIHPKNPYIPTSHANVRFFIA
EECCCCCHHHHCCCCCCCCCCCCCCHHCCCEEEECCEEEEEECCCCCCCCCCCCEEEEEE
EKEGEDPIWWFGGGFDLTPFYPVDEDCQSWHQTAKDLCAPFGDDVYQEHKEWCDKYFYLP
ECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HRDETRGVGGLFFDDLNEWGFEKSFAYMQAVGEGFAAAYLPIVERRKETPYGERERDFQL
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
YRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSMPPLARWEYRYEPQACSPEALLYSDYL
HHCCCEEEEEEEEECCEEEEECCCCCCHHHEECCCCCCCCEEEECCCCCCCCHHHHHHCC
KPRVW
CCCCC
>Mature Secondary Structure 
SAIDKEAVKQFLLSLQDSICQQLEQADGTALFEEDAWQREPGERLGGGGRTRVMTNGAV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHCCCCCEEEEECCCE
FEQGGVNFSHVAGKAMPASATAHRPELAGRKFEAMGVSLVIHPKNPYIPTSHANVRFFIA
EECCCCCHHHHCCCCCCCCCCCCCCHHCCCEEEECCEEEEEECCCCCCCCCCCCEEEEEE
EKEGEDPIWWFGGGFDLTPFYPVDEDCQSWHQTAKDLCAPFGDDVYQEHKEWCDKYFYLP
ECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HRDETRGVGGLFFDDLNEWGFEKSFAYMQAVGEGFAAAYLPIVERRKETPYGERERDFQL
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHH
YRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSMPPLARWEYRYEPQACSPEALLYSDYL
HHCCCEEEEEEEEECCEEEEECCCCCCHHHEECCCCCCCCEEEECCCCCCCCHHHHHHCC
KPRVW
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA