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The map label for this gene is thiE [H]

Identifier: 218710983

GI number: 218710983

Start: 3241147

End: 3242439

Strand: Reverse

Name: thiE [H]

Synonym: VS_3097

Alternate gene names: 218710983

Gene position: 3242439-3241147 (Counterclockwise)

Preceding gene: 218710984

Following gene: 218710982

Centisome position: 98.28

GC content: 45.09

Gene sequence:

>1293_bases
ATGACAGTGAAGATACTCATCCCATCTCAAAATATTGAGTTAACGGGAGAGGTGCAGAACTGTCTATTGGTTGCTAAGCG
ACAAGGCTTGGCAACCGATGCAGTTGAGTTGGGTGTAAGCCCAACTAAATACTTCTCTATCGTTGACACTCAGCAGGCGT
TATCTATTGGCTTTGCTTATGATGTTGATTCATTGGCGGAGTGTCAGTTGGCGGAACTGAACCATGTTGTTGATTACAGT
AATTCAGTCGCGCTAACTGACGTTTGTGATGCCTTTACACAAACTCCGAATACCATCTATATCGGTGTCTCAGATGATTC
AGTTGTGTTAGATATCTGGTCACACTTATATGCTAATCGTGCTATCAAGAGTGACACTACAGCCCATCAGGAGTTAGATA
ATCACGGCCACTTTGCCTGGTTACTTACTTTGTTGGCGTTGGAGTTCCCATTAGAAGACGCATTGGTTTTAGCTCGCGCA
TCGTCCAATGTTTCACGTGGAACATGGCCAGCACATTACCAAAACTTTCCAATTCCAATTTTGGAAGATGAGCGACTGGA
TATTAGTGTCGGTTGGGCGCACCAAGGAACATCACTTTCGTTCCCTGAATTGAGCAAGAGTAGCCTAGGGCTATACCCTG
TGGTTGATGATGTTGAGTGGATCGAAAGGTTGCTCAAGCTTGGAATCAACACTGTTCAACTACGTATTAAAAATCCTCTG
CAAGCGGACTTAGAACAACAAGTTGCGCGATCAATCGAGCTTGGTCGAGAGCATAACGCTCAGGTTTTTATCAATGATTA
CTGGCAGCTTGCACTCAAGCATGACGCGTTTGGTGTTCATTTGGGGCAAGAGGATATTGAAGAATCAAACCTCTCACAGT
TGAGCCGAGCAGGTATTAAGATAGGTCTCTCGACTCATGGCTACTACGAGTTGCTGCGCATTGTTCAAATCAACCCAAGC
TATATTGCGCTGGGACATATCTTCCCAACAACAACTAAACAGATGCCATCGAAACCTCAGGGTTTAGTTCGTTTATCTCT
GTATCAACAGCTGATCGATACTATCCCATACACAGATCAACTCACCGGTTACCCGACGGTCGCTATTGGTGGTATTGACC
AATCGAAAGCTGAGCAGGTGTGGGAGTGTGGGGTGTCTAGTTTGGCGGTGGTACGTGCGATTACATTGGCGGAAGACCCA
CAGAAAGTAATCGAATTTTTCGAAAAACTCATGGCACCTAAATCTCCAATTCTTAAGGAAGAGGTTATGCAGGAGCCTAG
CTATGCTGAGTGA

Upstream 100 bases:

>100_bases
CATTGGAAGGGATGCGTCAGAAATCACAAGAGTTCCGTGATACTGGGTCTGAACTTTACCACCCTGCAGTAGGTGCAAAA
GAAGCTCAATTAGAGGAATA

Downstream 100 bases:

>100_bases
CTTTGAATTCATTCGTTATCAACGACAAATTGCGTTACCTGAAGTGGGTGAGCAAGGGCAACGCAACCTACTAAATAGTC
ATGTGTTGGTCATCGGCTGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTVKILIPSQNIELTGEVQNCLLVAKRQGLATDAVELGVSPTKYFSIVDTQQALSIGFAYDVDSLAECQLAELNHVVDYS
NSVALTDVCDAFTQTPNTIYIGVSDDSVVLDIWSHLYANRAIKSDTTAHQELDNHGHFAWLLTLLALEFPLEDALVLARA
SSNVSRGTWPAHYQNFPIPILEDERLDISVGWAHQGTSLSFPELSKSSLGLYPVVDDVEWIERLLKLGINTVQLRIKNPL
QADLEQQVARSIELGREHNAQVFINDYWQLALKHDAFGVHLGQEDIEESNLSQLSRAGIKIGLSTHGYYELLRIVQINPS
YIALGHIFPTTTKQMPSKPQGLVRLSLYQQLIDTIPYTDQLTGYPTVAIGGIDQSKAEQVWECGVSSLAVVRAITLAEDP
QKVIEFFEKLMAPKSPILKEEVMQEPSYAE

Sequences:

>Translated_430_residues
MTVKILIPSQNIELTGEVQNCLLVAKRQGLATDAVELGVSPTKYFSIVDTQQALSIGFAYDVDSLAECQLAELNHVVDYS
NSVALTDVCDAFTQTPNTIYIGVSDDSVVLDIWSHLYANRAIKSDTTAHQELDNHGHFAWLLTLLALEFPLEDALVLARA
SSNVSRGTWPAHYQNFPIPILEDERLDISVGWAHQGTSLSFPELSKSSLGLYPVVDDVEWIERLLKLGINTVQLRIKNPL
QADLEQQVARSIELGREHNAQVFINDYWQLALKHDAFGVHLGQEDIEESNLSQLSRAGIKIGLSTHGYYELLRIVQINPS
YIALGHIFPTTTKQMPSKPQGLVRLSLYQQLIDTIPYTDQLTGYPTVAIGGIDQSKAEQVWECGVSSLAVVRAITLAEDP
QKVIEFFEKLMAPKSPILKEEVMQEPSYAE
>Mature_429_residues
TVKILIPSQNIELTGEVQNCLLVAKRQGLATDAVELGVSPTKYFSIVDTQQALSIGFAYDVDSLAECQLAELNHVVDYSN
SVALTDVCDAFTQTPNTIYIGVSDDSVVLDIWSHLYANRAIKSDTTAHQELDNHGHFAWLLTLLALEFPLEDALVLARAS
SNVSRGTWPAHYQNFPIPILEDERLDISVGWAHQGTSLSFPELSKSSLGLYPVVDDVEWIERLLKLGINTVQLRIKNPLQ
ADLEQQVARSIELGREHNAQVFINDYWQLALKHDAFGVHLGQEDIEESNLSQLSRAGIKIGLSTHGYYELLRIVQINPSY
IALGHIFPTTTKQMPSKPQGLVRLSLYQQLIDTIPYTDQLTGYPTVAIGGIDQSKAEQVWECGVSSLAVVRAITLAEDPQ
KVIEFFEKLMAPKSPILKEEVMQEPSYAE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=199, Percent_Identity=52.2613065326633, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 47798; Mature: 47667

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKILIPSQNIELTGEVQNCLLVAKRQGLATDAVELGVSPTKYFSIVDTQQALSIGFAY
CEEEEEECCCCCEEECHHHHHHHHHHHCCCCHHHHHHCCCCHHEEEEEECHHHHHEEEEE
DVDSLAECQLAELNHVVDYSNSVALTDVCDAFTQTPNTIYIGVSDDSVVLDIWSHLYANR
CCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCC
AIKSDTTAHQELDNHGHFAWLLTLLALEFPLEDALVLARASSNVSRGTWPAHYQNFPIPI
CCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCE
LEDERLDISVGWAHQGTSLSFPELSKSSLGLYPVVDDVEWIERLLKLGINTVQLRIKNPL
ECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEECCCH
QADLEQQVARSIELGREHNAQVFINDYWQLALKHDAFGVHLGQEDIEESNLSQLSRAGIK
HHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCEEEECCHHHHHHHHHHHHHHCCEE
IGLSTHGYYELLRIVQINPSYIALGHIFPTTTKQMPSKPQGLVRLSLYQQLIDTIPYTDQ
EEECCCCHHHEEEHEEECCCEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
LTGYPTVAIGGIDQSKAEQVWECGVSSLAVVRAITLAEDPQKVIEFFEKLMAPKSPILKE
CCCCCEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
EVMQEPSYAE
HHHCCCCCCC
>Mature Secondary Structure 
TVKILIPSQNIELTGEVQNCLLVAKRQGLATDAVELGVSPTKYFSIVDTQQALSIGFAY
EEEEEECCCCCEEECHHHHHHHHHHHCCCCHHHHHHCCCCHHEEEEEECHHHHHEEEEE
DVDSLAECQLAELNHVVDYSNSVALTDVCDAFTQTPNTIYIGVSDDSVVLDIWSHLYANR
CCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCC
AIKSDTTAHQELDNHGHFAWLLTLLALEFPLEDALVLARASSNVSRGTWPAHYQNFPIPI
CCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCE
LEDERLDISVGWAHQGTSLSFPELSKSSLGLYPVVDDVEWIERLLKLGINTVQLRIKNPL
ECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEECCCH
QADLEQQVARSIELGREHNAQVFINDYWQLALKHDAFGVHLGQEDIEESNLSQLSRAGIK
HHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCEEEECCHHHHHHHHHHHHHHCCEE
IGLSTHGYYELLRIVQINPSYIALGHIFPTTTKQMPSKPQGLVRLSLYQQLIDTIPYTDQ
EEECCCCHHHEEEHEEECCCEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
LTGYPTVAIGGIDQSKAEQVWECGVSSLAVVRAITLAEDPQKVIEFFEKLMAPKSPILKE
CCCCCEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
EVMQEPSYAE
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA