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The map label for this gene is thiF [H]

Identifier: 218710982

GI number: 218710982

Start: 3240375

End: 3241157

Strand: Reverse

Name: thiF [H]

Synonym: VS_3096

Alternate gene names: 218710982

Gene position: 3241157-3240375 (Counterclockwise)

Preceding gene: 218710983

Following gene: 218710981

Centisome position: 98.24

GC content: 46.23

Gene sequence:

>783_bases
ATGCTGAGTGACTTTGAATTCATTCGTTATCAACGACAAATTGCGTTACCTGAAGTGGGTGAGCAAGGGCAACGCAACCT
ACTAAATAGTCATGTGTTGGTCATCGGCTGTGGTGGCTTAGGTAATGCTGCGGCTCTCTACCTAGCTGCTTCTGGTGTTG
GAAAGATCGTGCTGGTCGATGATGATTGTGTCGATTCATCGAACCTGCAACGACAAGTCGCGTTCAGGGAAAATCAATTA
GGTTCGCCTAAGGTTGAAGCGTTGAAGCAACAATTGAGTGAGCTCAATGGTCGCAGCCAAGTAAGAACCATCAATCAACG
AATGAGTGAAAGTCAGCTTGAGCTTGAAGTGATGCTAGCTGACCTAGTGTTGGATTGTACTGACAACTTCGAGTCACGCC
AGCAGGTTAACCAAGCATGTTTCAGCACGAAGACCCCTTTGATTTCTGGTTCTGCAATTGGCTGGAAAGGCCAGTTTATT
GTATTTGATTATCAGAATCAGAAAGGGTGCTATCACTGCTTGTTCCCGTTCAGACAGCATCCCCAAACGACGCGTTGTAG
CGATAGCGGCATCATAGGCCCAGTTGTTGGCACCATAGGTAACCTTCAAGCTTTGGCTGCTATTCAACGTCTAACTCGTG
GTGAGTTTCAGGTCGCAACACACCAACTCAAACTGTTCGATGGCCAGACGATGAATTGGCAAAACCTTATGGTCACTCAA
GATAGTGAATGTCCTGTGTGCAGCGCTTCTGTAGTTAAGCATTTAGAAGAAGAAATCCAATGA

Upstream 100 bases:

>100_bases
ACATTGGCGGAAGACCCACAGAAAGTAATCGAATTTTTCGAAAAACTCATGGCACCTAAATCTCCAATTCTTAAGGAAGA
GGTTATGCAGGAGCCTAGCT

Downstream 100 bases:

>100_bases
GTCATATAACGATCTCGATAAACGAACAATCAGAACAAGTAGCACACTCTTCGTCTCTAGCGGACATTATCCAAGTTCTA
TCATTACCTGATCTAGGTTG

Product: hypothetical protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLSDFEFIRYQRQIALPEVGEQGQRNLLNSHVLVIGCGGLGNAAALYLAASGVGKIVLVDDDCVDSSNLQRQVAFRENQL
GSPKVEALKQQLSELNGRSQVRTINQRMSESQLELEVMLADLVLDCTDNFESRQQVNQACFSTKTPLISGSAIGWKGQFI
VFDYQNQKGCYHCLFPFRQHPQTTRCSDSGIIGPVVGTIGNLQALAAIQRLTRGEFQVATHQLKLFDGQTMNWQNLMVTQ
DSECPVCSASVVKHLEEEIQ

Sequences:

>Translated_260_residues
MLSDFEFIRYQRQIALPEVGEQGQRNLLNSHVLVIGCGGLGNAAALYLAASGVGKIVLVDDDCVDSSNLQRQVAFRENQL
GSPKVEALKQQLSELNGRSQVRTINQRMSESQLELEVMLADLVLDCTDNFESRQQVNQACFSTKTPLISGSAIGWKGQFI
VFDYQNQKGCYHCLFPFRQHPQTTRCSDSGIIGPVVGTIGNLQALAAIQRLTRGEFQVATHQLKLFDGQTMNWQNLMVTQ
DSECPVCSASVVKHLEEEIQ
>Mature_260_residues
MLSDFEFIRYQRQIALPEVGEQGQRNLLNSHVLVIGCGGLGNAAALYLAASGVGKIVLVDDDCVDSSNLQRQVAFRENQL
GSPKVEALKQQLSELNGRSQVRTINQRMSESQLELEVMLADLVLDCTDNFESRQQVNQACFSTKTPLISGSAIGWKGQFI
VFDYQNQKGCYHCLFPFRQHPQTTRCSDSGIIGPVVGTIGNLQALAAIQRLTRGEFQVATHQLKLFDGQTMNWQNLMVTQ
DSECPVCSASVVKHLEEEIQ

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=228, Percent_Identity=40.3508771929825, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI4885649, Length=159, Percent_Identity=28.9308176100629, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=45.7831325301205, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1787048, Length=246, Percent_Identity=40.2439024390244, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1789177, Length=136, Percent_Identity=25.7352941176471, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=241, Percent_Identity=38.5892116182573, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI193203301, Length=160, Percent_Identity=30.625, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321903, Length=210, Percent_Identity=36.1904761904762, Blast_Score=137, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322639, Length=161, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320598, Length=168, Percent_Identity=27.3809523809524, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321790, Length=144, Percent_Identity=24.3055555555556, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=40.6374501992032, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24660640, Length=148, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 28823; Mature: 28823

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDFEFIRYQRQIALPEVGEQGQRNLLNSHVLVIGCGGLGNAAALYLAASGVGKIVLVD
CCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHEEEECCCCEEEEEE
DDCVDSSNLQRQVAFRENQLGSPKVEALKQQLSELNGRSQVRTINQRMSESQLELEVMLA
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DLVLDCTDNFESRQQVNQACFSTKTPLISGSAIGWKGQFIVFDYQNQKGCYHCLFPFRQH
HHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCEEEECCHHCC
PQTTRCSDSGIIGPVVGTIGNLQALAAIQRLTRGEFQVATHQLKLFDGQTMNWQNLMVTQ
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCEEHHEEEEEECCCCCCCCEEEEEC
DSECPVCSASVVKHLEEEIQ
CCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSDFEFIRYQRQIALPEVGEQGQRNLLNSHVLVIGCGGLGNAAALYLAASGVGKIVLVD
CCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHEEEECCCCEEEEEE
DDCVDSSNLQRQVAFRENQLGSPKVEALKQQLSELNGRSQVRTINQRMSESQLELEVMLA
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DLVLDCTDNFESRQQVNQACFSTKTPLISGSAIGWKGQFIVFDYQNQKGCYHCLFPFRQH
HHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCEEEECCHHCC
PQTTRCSDSGIIGPVVGTIGNLQALAAIQRLTRGEFQVATHQLKLFDGQTMNWQNLMVTQ
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCEEHHEEEEEECCCCCCCCEEEEEC
DSECPVCSASVVKHLEEEIQ
CCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]