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The map label for this gene is thiG

Identifier: 218710980

GI number: 218710980

Start: 3239402

End: 3240166

Strand: Reverse

Name: thiG

Synonym: VS_3094

Alternate gene names: 218710980

Gene position: 3240166-3239402 (Counterclockwise)

Preceding gene: 218710981

Following gene: 218710979

Centisome position: 98.21

GC content: 48.1

Gene sequence:

>765_bases
ATGCTAACTATCGCAGATAAAACGTTTCAATCTCGACTCTTCACTGGAACTGGTAAATTCGCCAATAAGCACTTGATGGC
GAGTGCTATCGAAGCTTCCGGTTCTCAACTGGCAACCATGGCGTTGAAGAGGGTTGATATTCGCTCTGAGCAAGACGACA
TTTTACAACCCATCATTGATGCTGGCGTGAACTTACTACCGAATACATCAGGTGCTAAGAATGCAAAAGACGCCATCTTT
GCTGCCCACTTGGCTCGTGAAGCGCTAGGCACAAATTGGCTTAAACTGGAAATTCACCCAGATCCAAAGTACTTGATGCC
GGACCCAATTGAAACGCTTAAAGCTGCTGAGCAACTGGTCAAAGATGGCTTTGTTGTGTTGCCTTATTGCCATGCCGACC
CTGTTTTGTGTAAGCGTTTGGAAGAGGTAGGTTGTGCGGCAGTTATGCCGTTGGGTGCGCCTATTGGTTCAAATAAGGGT
ATTGCGTCTGCAGACTTCTTAGAGATCATTATCGACCAAGCGAATGTCCCTGTGATTGTTGATGCGGGCATTGGCGCGCC
ATCACATGCTGCACGTGCAATGGAAATGGGCGCAGACGCAGTGTTAGTGAATACCGCAATTGCTGCTTCTCAACAGCCGG
TTGAAATGGCAATTGCGTTTAAGTTGGCAGTTGAAGCGGGGCGTATGGCTTACCTTGCTGGACTTGCAGGAAAAGTATCT
CACGCGGTTGCTTCAAGCCCGTTAACTTCATTCCTAGACGAGTAG

Upstream 100 bases:

>100_bases
CAATCAATAATGCGGTTGTCCCGCGTAGTCAGTGGCAACAAACGGTCGTTAATGAAGGCGACGCTATTTCTCTTTTCCAA
GCCATTGCAGGAGGCTAACC

Downstream 100 bases:

>100_bases
CAGTGCTATGACGTTTGTTGATCGATTTAAACAGCTCAACTGGGATGACATTGGTATGTCTATCTTCAGTAAAACGGCAG
CGGATGTTGAACGTGCTCTG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLTIADKTFQSRLFTGTGKFANKHLMASAIEASGSQLATMALKRVDIRSEQDDILQPIIDAGVNLLPNTSGAKNAKDAIF
AAHLAREALGTNWLKLEIHPDPKYLMPDPIETLKAAEQLVKDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKG
IASADFLEIIIDQANVPVIVDAGIGAPSHAARAMEMGADAVLVNTAIAASQQPVEMAIAFKLAVEAGRMAYLAGLAGKVS
HAVASSPLTSFLDE

Sequences:

>Translated_254_residues
MLTIADKTFQSRLFTGTGKFANKHLMASAIEASGSQLATMALKRVDIRSEQDDILQPIIDAGVNLLPNTSGAKNAKDAIF
AAHLAREALGTNWLKLEIHPDPKYLMPDPIETLKAAEQLVKDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKG
IASADFLEIIIDQANVPVIVDAGIGAPSHAARAMEMGADAVLVNTAIAASQQPVEMAIAFKLAVEAGRMAYLAGLAGKVS
HAVASSPLTSFLDE
>Mature_254_residues
MLTIADKTFQSRLFTGTGKFANKHLMASAIEASGSQLATMALKRVDIRSEQDDILQPIIDAGVNLLPNTSGAKNAKDAIF
AAHLAREALGTNWLKLEIHPDPKYLMPDPIETLKAAEQLVKDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKG
IASADFLEIIIDQANVPVIVDAGIGAPSHAARAMEMGADAVLVNTAIAASQQPVEMAIAFKLAVEAGRMAYLAGLAGKVS
HAVASSPLTSFLDE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=73.1225296442688, Blast_Score=376, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_VIBSL (B7VMV3)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418601.1
- ProteinModelPortal:   B7VMV3
- GeneID:   7162547
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3094
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 26695; Mature: 26695

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIADKTFQSRLFTGTGKFANKHLMASAIEASGSQLATMALKRVDIRSEQDDILQPIID
CCEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AGVNLLPNTSGAKNAKDAIFAAHLAREALGTNWLKLEIHPDPKYLMPDPIETLKAAEQLV
CCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
KDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKGIASADFLEIIIDQANVPVIV
HCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
DAGIGAPSHAARAMEMGADAVLVNTAIAASQQPVEMAIAFKLAVEAGRMAYLAGLAGKVS
ECCCCCCHHHHHHHHHCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
HAVASSPLTSFLDE
HHHHHCHHHHHHCC
>Mature Secondary Structure
MLTIADKTFQSRLFTGTGKFANKHLMASAIEASGSQLATMALKRVDIRSEQDDILQPIID
CCEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AGVNLLPNTSGAKNAKDAIFAAHLAREALGTNWLKLEIHPDPKYLMPDPIETLKAAEQLV
CCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
KDGFVVLPYCHADPVLCKRLEEVGCAAVMPLGAPIGSNKGIASADFLEIIIDQANVPVIV
HCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
DAGIGAPSHAARAMEMGADAVLVNTAIAASQQPVEMAIAFKLAVEAGRMAYLAGLAGKVS
ECCCCCCHHHHHHHHHCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
HAVASSPLTSFLDE
HHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA