Click here to switch to the map view.

The map label for this gene is thiH [H]

Identifier: 218710979

GI number: 218710979

Start: 3238267

End: 3239394

Strand: Reverse

Name: thiH [H]

Synonym: VS_3093

Alternate gene names: 218710979

Gene position: 3239394-3238267 (Counterclockwise)

Preceding gene: 218710980

Following gene: 218710976

Centisome position: 98.18

GC content: 46.45

Gene sequence:

>1128_bases
ATGACGTTTGTTGATCGATTTAAACAGCTCAACTGGGATGACATTGGTATGTCTATCTTCAGTAAAACGGCAGCGGATGT
TGAACGTGCTCTGAGTAAACCCAAACGTGACTTAGAAGACTTTAAGGCTCTGATCTCTCCGGCGGCAGAACCTTACTTAG
AGCAGATGGCACAACAATCGTTGGCGCTAACTCGTAAGCGATTTGGCAATACAATGTCGCTTTATATTCCTTTGTACCTA
TCTAACCTGTGCGCTAACGCGTGCACGTATTGTGGCTTCTCAATGGAGAACCGTATCAAGCGCCGCACGCTTACTTTGGA
TGAAATTGATGCCGAAAGTGCGGCTATCAAAAAGATGAAGTTCGATAGCGTTTTGTTGGTCACCGGTGAACATGAAACTA
AGGTCGGGATGAATTACTTTCGACAGGTGTTGCCGAATATCAAAAAGCAATTCAACTACCTAGCGATGGAAGTGCAGCCG
CTTGACCAACAAGATTACGCAGAACTTAAAACGCTCGGTTTAGATGCGGTGATGGTTTATCAAGAGACCTATCAACCAAG
GACCTACGCTGAGCATCATCTACGTGGTAATAAAATGGATTTTGAATATCGTCTTGAAACCCCTGATCGCTTGGCAAAGG
CGGGGATCGATAAGATAGGGATTGGTGCCTTGATTGGTTTGGAAGATTGGCGAACCGACTGCTTTTTTGTTGCTGCTCAC
TTAGATTACCTAGAGCGCACTTATTGGCAGACTCGCTATTCAATTTCATTCCCGCGTCTTCGTCCGTGTGAGGGTGGAGA
CTCTAGTGGAGGATTACAGCCTAAATCAGTCATGAACGACAAACAATTGGTTCAGCTTATCTGCGCATATCGACTCTTGA
ATCCTGAGGTCGAGCTATCCCTTTCTACTCGCGAATCGGCAACCTTTCGCGACAATGTGTTGCCATTGGGGATCACGAGT
ATGTCTGCGGCTTCTAAAACCCAACCTGGTGGTTATGCTTCGGGTGAAGAAGAGCTTGAGCAGTTTGAGATCAGTGATGA
GAGAAGTGCTGATGATGTCGAGTCGATGATTCGTCAGCGTGGTTTCGACCCAGTGTGGCGAGACTGGCACAGAGCCTATT
CTGGTTAA

Upstream 100 bases:

>100_bases
GAAGCGGGGCGTATGGCTTACCTTGCTGGACTTGCAGGAAAAGTATCTCACGCGGTTGCTTCAAGCCCGTTAACTTCATT
CCTAGACGAGTAGCAGTGCT

Downstream 100 bases:

>100_bases
TCAACATATTGGTTGATGTTCCACGTGAAACATCGATGTAATGATACAGGCAAATAAAAACGGCTACCCTTGGGTAGCCG
TTTTGCTATTTGCTATTTAA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTFVDRFKQLNWDDIGMSIFSKTAADVERALSKPKRDLEDFKALISPAAEPYLEQMAQQSLALTRKRFGNTMSLYIPLYL
SNLCANACTYCGFSMENRIKRRTLTLDEIDAESAAIKKMKFDSVLLVTGEHETKVGMNYFRQVLPNIKKQFNYLAMEVQP
LDQQDYAELKTLGLDAVMVYQETYQPRTYAEHHLRGNKMDFEYRLETPDRLAKAGIDKIGIGALIGLEDWRTDCFFVAAH
LDYLERTYWQTRYSISFPRLRPCEGGDSSGGLQPKSVMNDKQLVQLICAYRLLNPEVELSLSTRESATFRDNVLPLGITS
MSAASKTQPGGYASGEEELEQFEISDERSADDVESMIRQRGFDPVWRDWHRAYSG

Sequences:

>Translated_375_residues
MTFVDRFKQLNWDDIGMSIFSKTAADVERALSKPKRDLEDFKALISPAAEPYLEQMAQQSLALTRKRFGNTMSLYIPLYL
SNLCANACTYCGFSMENRIKRRTLTLDEIDAESAAIKKMKFDSVLLVTGEHETKVGMNYFRQVLPNIKKQFNYLAMEVQP
LDQQDYAELKTLGLDAVMVYQETYQPRTYAEHHLRGNKMDFEYRLETPDRLAKAGIDKIGIGALIGLEDWRTDCFFVAAH
LDYLERTYWQTRYSISFPRLRPCEGGDSSGGLQPKSVMNDKQLVQLICAYRLLNPEVELSLSTRESATFRDNVLPLGITS
MSAASKTQPGGYASGEEELEQFEISDERSADDVESMIRQRGFDPVWRDWHRAYSG
>Mature_374_residues
TFVDRFKQLNWDDIGMSIFSKTAADVERALSKPKRDLEDFKALISPAAEPYLEQMAQQSLALTRKRFGNTMSLYIPLYLS
NLCANACTYCGFSMENRIKRRTLTLDEIDAESAAIKKMKFDSVLLVTGEHETKVGMNYFRQVLPNIKKQFNYLAMEVQPL
DQQDYAELKTLGLDAVMVYQETYQPRTYAEHHLRGNKMDFEYRLETPDRLAKAGIDKIGIGALIGLEDWRTDCFFVAAHL
DYLERTYWQTRYSISFPRLRPCEGGDSSGGLQPKSVMNDKQLVQLICAYRLLNPEVELSLSTRESATFRDNVLPLGITSM
SAASKTQPGGYASGEEELEQFEISDERSADDVESMIRQRGFDPVWRDWHRAYSG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=369, Percent_Identity=62.6016260162602, Blast_Score=487, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42921; Mature: 42790

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFVDRFKQLNWDDIGMSIFSKTAADVERALSKPKRDLEDFKALISPAAEPYLEQMAQQS
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LALTRKRFGNTMSLYIPLYLSNLCANACTYCGFSMENRIKRRTLTLDEIDAESAAIKKMK
HHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCHHHHHHHCC
FDSVLLVTGEHETKVGMNYFRQVLPNIKKQFNYLAMEVQPLDQQDYAELKTLGLDAVMVY
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCHHEEEE
QETYQPRTYAEHHLRGNKMDFEYRLETPDRLAKAGIDKIGIGALIGLEDWRTDCFFVAAH
HHHCCCHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCHHEECCHHHCCHHHHHHHH
LDYLERTYWQTRYSISFPRLRPCEGGDSSGGLQPKSVMNDKQLVQLICAYRLLNPEVELS
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEE
LSTRESATFRDNVLPLGITSMSAASKTQPGGYASGEEELEQFEISDERSADDVESMIRQR
EECCCCCCHHCCCCEECCCHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHC
GFDPVWRDWHRAYSG
CCCHHHHHHHHHHCC
>Mature Secondary Structure 
TFVDRFKQLNWDDIGMSIFSKTAADVERALSKPKRDLEDFKALISPAAEPYLEQMAQQS
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
LALTRKRFGNTMSLYIPLYLSNLCANACTYCGFSMENRIKRRTLTLDEIDAESAAIKKMK
HHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCHHHHHHHCC
FDSVLLVTGEHETKVGMNYFRQVLPNIKKQFNYLAMEVQPLDQQDYAELKTLGLDAVMVY
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCHHEEEE
QETYQPRTYAEHHLRGNKMDFEYRLETPDRLAKAGIDKIGIGALIGLEDWRTDCFFVAAH
HHHCCCHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCHHEECCHHHCCHHHHHHHH
LDYLERTYWQTRYSISFPRLRPCEGGDSSGGLQPKSVMNDKQLVQLICAYRLLNPEVELS
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEE
LSTRESATFRDNVLPLGITSMSAASKTQPGGYASGEEELEQFEISDERSADDVESMIRQR
EECCCCCCHHCCCCEECCCHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHC
GFDPVWRDWHRAYSG
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]