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The map label for this gene is mdtL [H]

Identifier: 218710976

GI number: 218710976

Start: 3233962

End: 3235284

Strand: Reverse

Name: mdtL [H]

Synonym: VS_3090

Alternate gene names: 218710976

Gene position: 3235284-3233962 (Counterclockwise)

Preceding gene: 218710979

Following gene: 218710975

Centisome position: 98.06

GC content: 45.58

Gene sequence:

>1323_bases
GTGATAGTTTCTCAATATTCATGTGAGTTTCCTATTTGGAATAATGCTAATGAGTATATGCCATTTTTGTTCATATCTTT
AGTTGGTTATTATGCACCTAAATTAACTCATTCGGAGAATCTTGTGCCTTCTAACGCGCTTCCAACCCCAAGTAAACTGC
AGGTTGCTTTATTGGCAATGCTGGTTCTATTTAGCCCTTTGGCTATAGATATTTATCTTCCAGCTCTGCCACAAATTTCA
ACAGCGTTTCACGTGGAACACGCACTAGCACAAGATACGATTACCTGGTTTTTATTCGCTATGGGTGTCGGGCAATTATT
TGCTGGCCCTTTGGCTGATAAGTTAGGACGTCGAACCGTTGCATTGGGAGGTGTTAGTATCTATGCATTGAGTGCTTGCT
TGGCGTGGGCAGCTCAATCGATTGATATGATGCTAATGGCTCGGCTGCTACAAGGCTTAGGAGCTTGTGCGACTTCTGTA
GCAGCCTTTGCAACGGTTCGCGATCTATTTGGTCCAGAGAAGAGTGGCCGTATGATCAGCTACCTTAATGGTGCAATCTG
TTTTATTCCTGCTTTGGCACCTATTCTTGGCGCTTGGTTAACTCATCAATTTGGTTGGCGTTCGAACTTTAGCTTTATGG
CAGGCTTCGCTGTCGTTGTTGGCACTATCTTATTTTTCCAAATGAAAGAGTCTAACCCTGCGACAGAAAAGGTTGCGGTA
TTCAAGCTTGAGCGATACTGGTCAGTATTGAAAACACCATCATTCCTTTTCCACGCAACTTTGTGTCTGATGGCGATGGC
GGTTATCCTCGCTTATGTAACCTCTGCACCAGTTGTGTTGATGGAAAACCTAGGCCTGACCATGAAAGAGTTTACTTTCT
GGTTTGGTGTTAATGCGGCCATCAACATTGCAGCTGCGTTTACTGCACCTAAATTTATGGACCGTTTTGGTACCTATAAA
GCACTGGTCGTTGGTATCTCAACGCTGGGTTTAGCGGGCGTGATTATGTTAGTGCTTGCAGAACACGCTAGCCCAATCGC
ATTCATGCTGCCAATCTTCTTGTCTTCAGTGGGTTTTGCTTGGATTCTCGGTGCTGCTGCTGGTAAGGCTCTTGAACCTT
TCGGTGACCGTGCGGGCACAGCTGCTGCACTGCTTGGCTTGTTCCAAATGAGTGGATCAGGGCTGCTTGTTGGTACCATG
CAACGCCTTGATTTAACGTCTCAAGTGATGATCGCGCTGCAAATGTTCCTTATTGTTCCAGCATTGTTAGTGCTTGCGAG
TAAAGCAGGCAAGTCGTGGCACACGACGTTTGCTAAGGCGTAA

Upstream 100 bases:

>100_bases
CTCACAGCCGATTGGCTCAGGCACAATCGATGTGCGGTGCGCGTGACGCTTAGCTCTTCCATCAGCACTTGTAAGCAAAC
CAGTAGGTTTAAGTCGAGGC

Downstream 100 bases:

>100_bases
GTCTCAAGGGTGACAAAATATACAAATGGCGGTTTGCGAAACGTTAAAACATGGTATATTTGTCACTCATTGAAACGTTA
GTCCCCAACGGAAAATACTG

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIVSQYSCEFPIWNNANEYMPFLFISLVGYYAPKLTHSENLVPSNALPTPSKLQVALLAMLVLFSPLAIDIYLPALPQIS
TAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTVALGGVSIYALSACLAWAAQSIDMMLMARLLQGLGACATSV
AAFATVRDLFGPEKSGRMISYLNGAICFIPALAPILGAWLTHQFGWRSNFSFMAGFAVVVGTILFFQMKESNPATEKVAV
FKLERYWSVLKTPSFLFHATLCLMAMAVILAYVTSAPVVLMENLGLTMKEFTFWFGVNAAINIAAAFTAPKFMDRFGTYK
ALVVGISTLGLAGVIMLVLAEHASPIAFMLPIFLSSVGFAWILGAAAGKALEPFGDRAGTAAALLGLFQMSGSGLLVGTM
QRLDLTSQVMIALQMFLIVPALLVLASKAGKSWHTTFAKA

Sequences:

>Translated_440_residues
MIVSQYSCEFPIWNNANEYMPFLFISLVGYYAPKLTHSENLVPSNALPTPSKLQVALLAMLVLFSPLAIDIYLPALPQIS
TAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTVALGGVSIYALSACLAWAAQSIDMMLMARLLQGLGACATSV
AAFATVRDLFGPEKSGRMISYLNGAICFIPALAPILGAWLTHQFGWRSNFSFMAGFAVVVGTILFFQMKESNPATEKVAV
FKLERYWSVLKTPSFLFHATLCLMAMAVILAYVTSAPVVLMENLGLTMKEFTFWFGVNAAINIAAAFTAPKFMDRFGTYK
ALVVGISTLGLAGVIMLVLAEHASPIAFMLPIFLSSVGFAWILGAAAGKALEPFGDRAGTAAALLGLFQMSGSGLLVGTM
QRLDLTSQVMIALQMFLIVPALLVLASKAGKSWHTTFAKA
>Mature_440_residues
MIVSQYSCEFPIWNNANEYMPFLFISLVGYYAPKLTHSENLVPSNALPTPSKLQVALLAMLVLFSPLAIDIYLPALPQIS
TAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTVALGGVSIYALSACLAWAAQSIDMMLMARLLQGLGACATSV
AAFATVRDLFGPEKSGRMISYLNGAICFIPALAPILGAWLTHQFGWRSNFSFMAGFAVVVGTILFFQMKESNPATEKVAV
FKLERYWSVLKTPSFLFHATLCLMAMAVILAYVTSAPVVLMENLGLTMKEFTFWFGVNAAINIAAAFTAPKFMDRFGTYK
ALVVGISTLGLAGVIMLVLAEHASPIAFMLPIFLSSVGFAWILGAAAGKALEPFGDRAGTAAALLGLFQMSGSGLLVGTM
QRLDLTSQVMIALQMFLIVPALLVLASKAGKSWHTTFAKA

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=335, Percent_Identity=32.5373134328358, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1788509, Length=368, Percent_Identity=28.5326086956522, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87082312, Length=349, Percent_Identity=28.080229226361, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI48994889, Length=353, Percent_Identity=27.1954674220963, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790794, Length=261, Percent_Identity=27.2030651340996, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787065, Length=194, Percent_Identity=28.3505154639175, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=160, Percent_Identity=28.75, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321838, Length=185, Percent_Identity=25.4054054054054, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319517, Length=204, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322070, Length=190, Percent_Identity=28.4210526315789, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322071, Length=208, Percent_Identity=28.8461538461538, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47347; Mature: 47347

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSQYSCEFPIWNNANEYMPFLFISLVGYYAPKLTHSENLVPSNALPTPSKLQVALLAM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LVLFSPLAIDIYLPALPQISTAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTV
HHHHCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ALGGVSIYALSACLAWAAQSIDMMLMARLLQGLGACATSVAAFATVRDLFGPEKSGRMIS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
YLNGAICFIPALAPILGAWLTHQFGWRSNFSFMAGFAVVVGTILFFQMKESNPATEKVAV
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FKLERYWSVLKTPSFLFHATLCLMAMAVILAYVTSAPVVLMENLGLTMKEFTFWFGVNAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCHH
INIAAAFTAPKFMDRFGTYKALVVGISTLGLAGVIMLVLAEHASPIAFMLPIFLSSVGFA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
WILGAAAGKALEPFGDRAGTAAALLGLFQMSGSGLLVGTMQRLDLTSQVMIALQMFLIVP
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ALLVLASKAGKSWHTTFAKA
HHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MIVSQYSCEFPIWNNANEYMPFLFISLVGYYAPKLTHSENLVPSNALPTPSKLQVALLAM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LVLFSPLAIDIYLPALPQISTAFHVEHALAQDTITWFLFAMGVGQLFAGPLADKLGRRTV
HHHHCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ALGGVSIYALSACLAWAAQSIDMMLMARLLQGLGACATSVAAFATVRDLFGPEKSGRMIS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
YLNGAICFIPALAPILGAWLTHQFGWRSNFSFMAGFAVVVGTILFFQMKESNPATEKVAV
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FKLERYWSVLKTPSFLFHATLCLMAMAVILAYVTSAPVVLMENLGLTMKEFTFWFGVNAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCHH
INIAAAFTAPKFMDRFGTYKALVVGISTLGLAGVIMLVLAEHASPIAFMLPIFLSSVGFA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
WILGAAAGKALEPFGDRAGTAAALLGLFQMSGSGLLVGTMQRLDLTSQVMIALQMFLIVP
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ALLVLASKAGKSWHTTFAKA
HHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA