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The map label for this gene is uvrD [H]

Identifier: 218710970

GI number: 218710970

Start: 3228644

End: 3230818

Strand: Reverse

Name: uvrD [H]

Synonym: VS_3084

Alternate gene names: 218710970

Gene position: 3230818-3228644 (Counterclockwise)

Preceding gene: 218710975

Following gene: 218710963

Centisome position: 97.92

GC content: 48.41

Gene sequence:

>2175_bases
ATGATAGATCCTTCGCTTTTACTCGATGGCCTAAACGACAAACAACGTGAGGCGGTCGCAGCACCTTTAGAAAACCTACT
TATTCTGGCAGGTGCTGGCAGTGGTAAAACGCGAGTGTTGGTGCATCGTATCGCTTGGTTACAAAGCGTAGAGCAAGCAT
CACCGTTCTCTATCATGTCGGTTACCTTCACCAACAAAGCGGCAGCAGAGATGCGTGGTCGTATTGAAGAGTTGATGATG
GGTAGCTCGTCTGGTATGTGGAACGGCACCTTCCACGGTATCTGTCACCGCATTCTTCGTGCTCACTACCTAGATGCAAA
ACTGCCAGAAGATTTCCAGATCATTGATTCAGATGATCAGATTCGTTTGCTGCGTCGCTTAATCAAGGCGCAAAACCTTG
ATGAAAAACAGTGGCCAGCCAAGCAAGCTTCTTGGTGGATCAATGGCAAGAAAGACGAAGGGCTGCGCCCAAGTCACATT
GACGCCTACCATGATCCAATAACTCAAACGTGGTTAAAGATTTACTCTGCTTACCAAGAGGCATGTGATCGTGCTGGCTT
GGTCGACTTTGCTGAAATCTTGTTGCGATCGCATGAGCTATTGCGCGATAAGAAACACATCCGCGAGCACTACCAAGCTC
GCTTTAAGCACATTCTTGTCGACGAATTCCAAGATACCAACAACATCCAATACGCTTGGCTTCGCATGATGGCAGGCCCG
GATTGTCGCGTGATGATCGTGGGTGATGATGACCAATCTATCTATGGCTGGCGTGGCGCTAAAATTGAAAATATTCAGAA
GTTCTTGGATGAATTCCCAGGCGCTTCAACGGTTCGACTCGAACAAAACTACCGTTCAACCAAAACCATTCTGCAGGCGT
CGAATGAGCTTATCTCGAACAACACTGAGCGTATGGGTAAAGAGCTGTGGACTGATGGCAACGATGGCGAGCCAATCTCT
GTTTACTCAGCTTATAACGAGTTAGATGAAGCGCGTTTCACAGTCAGTAAAATCAAAGAGTGGCAAGAGAAAGGCGGTGC
GCTAGAGGATACCGCGATGCTGTATCGTAATAACGCCCAATCGCGTGTTCTTGAAGAAGCTTTGATTCAAGGTGGTCTAC
CTTACCGAATCTATGGCGGTATGCGATTCTTCGAGCGTCAAGAAATCAGAGATGCCTTGAGCTACCTGCGCTTAATGAGT
AACCGCAGCAATGATGCGGCATTCGAGCGTGTCGTCAATACGCCCACTCGTGGCTTGGGTGATAAAACGTTAGAAACGAT
TCGTCTTGCAGCACGTGATCGTGGTGCAACCATGTGGGAAGCCAGTGTTGCTCTGATAGAAGAACAAGTATTACCAGGCC
GTGCTGCGGGTGCCTTGAGCCGCTTTATTGAGCTTATCAATGCACTTGAAGATGACACCATAGAACTGAGACTTCATGAG
CAAACCGACCACGTGATCAAATCGTCGGGCTTGTTTGCGATGTATGAGCAAGAGAAAGGCGAGAAGTCGAAGGCACGTAT
TGAGAACTTGGAAGAATTGGTAACGGCAACGCGTCAGTTTGAAAAACCAGAAGAAGCGGATGAAATGAGCATGCTAACGG
CATTCTTAACTCATGCGGCTTTGGAAGCGGGTGAAGGTCAGGCTGATGAATTTGATGATGCGGTTCAGCTCATGACTCTG
CACAGTGCCAAAGGCCTAGAGTTCCCAATGGTATTCATGGTGGGTGTCGAAGAAGGCATGTTCCCAAGCCAGATGTCGGC
CGAAGAAGCAGGGCGTTTAGAAGAAGAGCGCCGCTTGTGTTACGTAGGTATGACTCGTGCGATGGAGAAGCTTTACATCA
CTTATGCTGAGATGCGTCGTTTGTACGGTCAAGATAAATACCATAAGCCATCACGCTTTATTCGTGAGTTGCCGGAGACC
TGTCTGGATGAAGTACGTATGAAAGCGCAAGTCAGCCGCCCTGCAAGCAGTGGTCGCTTTAGCCAAACCGCTGTGAAAGA
GAACTTCAATGAAACAGGCTTTAGCTTGGGTTCTCGCGTGAAGCATCCTAAGTTTGGTGAAGGCACTATCATCAACTTCG
AAGGAAGTGGCCCACAAAGCCGAGTTCAGGTGGCGTTTAACGGTGAAGGGATTAAGTGGTTAGTAACGGCTTACGCGCGT
TTAGAGCAGCTTTAA

Upstream 100 bases:

>100_bases
TAATTTTTGAATGTTTTTTCTTCTTATTATCCTAAACGCAGCCTAGTGTTTAGGTTATAATCAACTTTTATGTATAAATA
CCCAGTAGTGAGCATATCCA

Downstream 100 bases:

>100_bases
TCTATTATTTCAGCATCTAATAACCGTTCATTATTGAAAACCAAAAGAGCAGCCAATGGCTGCTCTTTTTTTATTGCGTA
CCGCTTTAAGTGATCACAAT

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 724; Mature: 724

Protein sequence:

>724_residues
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLQSVEQASPFSIMSVTFTNKAAAEMRGRIEELMM
GSSSGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQIRLLRRLIKAQNLDEKQWPAKQASWWINGKKDEGLRPSHI
DAYHDPITQTWLKIYSAYQEACDRAGLVDFAEILLRSHELLRDKKHIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
DCRVMIVGDDDQSIYGWRGAKIENIQKFLDEFPGASTVRLEQNYRSTKTILQASNELISNNTERMGKELWTDGNDGEPIS
VYSAYNELDEARFTVSKIKEWQEKGGALEDTAMLYRNNAQSRVLEEALIQGGLPYRIYGGMRFFERQEIRDALSYLRLMS
NRSNDAAFERVVNTPTRGLGDKTLETIRLAARDRGATMWEASVALIEEQVLPGRAAGALSRFIELINALEDDTIELRLHE
QTDHVIKSSGLFAMYEQEKGEKSKARIENLEELVTATRQFEKPEEADEMSMLTAFLTHAALEAGEGQADEFDDAVQLMTL
HSAKGLEFPMVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMEKLYITYAEMRRLYGQDKYHKPSRFIRELPET
CLDEVRMKAQVSRPASSGRFSQTAVKENFNETGFSLGSRVKHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
LEQL

Sequences:

>Translated_724_residues
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLQSVEQASPFSIMSVTFTNKAAAEMRGRIEELMM
GSSSGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQIRLLRRLIKAQNLDEKQWPAKQASWWINGKKDEGLRPSHI
DAYHDPITQTWLKIYSAYQEACDRAGLVDFAEILLRSHELLRDKKHIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
DCRVMIVGDDDQSIYGWRGAKIENIQKFLDEFPGASTVRLEQNYRSTKTILQASNELISNNTERMGKELWTDGNDGEPIS
VYSAYNELDEARFTVSKIKEWQEKGGALEDTAMLYRNNAQSRVLEEALIQGGLPYRIYGGMRFFERQEIRDALSYLRLMS
NRSNDAAFERVVNTPTRGLGDKTLETIRLAARDRGATMWEASVALIEEQVLPGRAAGALSRFIELINALEDDTIELRLHE
QTDHVIKSSGLFAMYEQEKGEKSKARIENLEELVTATRQFEKPEEADEMSMLTAFLTHAALEAGEGQADEFDDAVQLMTL
HSAKGLEFPMVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMEKLYITYAEMRRLYGQDKYHKPSRFIRELPET
CLDEVRMKAQVSRPASSGRFSQTAVKENFNETGFSLGSRVKHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
LEQL
>Mature_724_residues
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLQSVEQASPFSIMSVTFTNKAAAEMRGRIEELMM
GSSSGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQIRLLRRLIKAQNLDEKQWPAKQASWWINGKKDEGLRPSHI
DAYHDPITQTWLKIYSAYQEACDRAGLVDFAEILLRSHELLRDKKHIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
DCRVMIVGDDDQSIYGWRGAKIENIQKFLDEFPGASTVRLEQNYRSTKTILQASNELISNNTERMGKELWTDGNDGEPIS
VYSAYNELDEARFTVSKIKEWQEKGGALEDTAMLYRNNAQSRVLEEALIQGGLPYRIYGGMRFFERQEIRDALSYLRLMS
NRSNDAAFERVVNTPTRGLGDKTLETIRLAARDRGATMWEASVALIEEQVLPGRAAGALSRFIELINALEDDTIELRLHE
QTDHVIKSSGLFAMYEQEKGEKSKARIENLEELVTATRQFEKPEEADEMSMLTAFLTHAALEAGEGQADEFDDAVQLMTL
HSAKGLEFPMVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMEKLYITYAEMRRLYGQDKYHKPSRFIRELPET
CLDEVRMKAQVSRPASSGRFSQTAVKENFNETGFSLGSRVKHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
LEQL

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=723, Percent_Identity=70.5394190871369, Blast_Score=1084, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=646, Percent_Identity=37.9256965944272, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=26.9005847953216, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=737, Percent_Identity=27.9511533242877, Blast_Score=190, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324477, Length=674, Percent_Identity=20.919881305638, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82404; Mature: 82404

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLQSVEQASPFSIMS
CCCHHHHHCCCCHHHHHHHHHCHHCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEE
VTFTNKAAAEMRGRIEELMMGSSSGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQ
EEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHH
IRLLRRLIKAQNLDEKQWPAKQASWWINGKKDEGLRPSHIDAYHDPITQTWLKIYSAYQE
HHHHHHHHHHCCCCHHHCCCHHCCEEECCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHH
ACDRAGLVDFAEILLRSHELLRDKKHIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
DCRVMIVGDDDQSIYGWRGAKIENIQKFLDEFPGASTVRLEQNYRSTKTILQASNELISN
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCC
NTERMGKELWTDGNDGEPISVYSAYNELDEARFTVSKIKEWQEKGGALEDTAMLYRNNAQ
CHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
SRVLEEALIQGGLPYRIYGGMRFFERQEIRDALSYLRLMSNRSNDAAFERVVNTPTRGLG
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DKTLETIRLAARDRGATMWEASVALIEEQVLPGRAAGALSRFIELINALEDDTIELRLHE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
QTDHVIKSSGLFAMYEQEKGEKSKARIENLEELVTATRQFEKPEEADEMSMLTAFLTHAA
HHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LEAGEGQADEFDDAVQLMTLHSAKGLEFPMVFMVGVEEGMFPSQMSAEEAGRLEEERRLC
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCHHHHHHHH
YVGMTRAMEKLYITYAEMRRLYGQDKYHKPSRFIRELPETCLDEVRMKAQVSRPASSGRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SQTAVKENFNETGFSLGSRVKHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
LEQL
HHCC
>Mature Secondary Structure
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLQSVEQASPFSIMS
CCCHHHHHCCCCHHHHHHHHHCHHCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEE
VTFTNKAAAEMRGRIEELMMGSSSGMWNGTFHGICHRILRAHYLDAKLPEDFQIIDSDDQ
EEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHH
IRLLRRLIKAQNLDEKQWPAKQASWWINGKKDEGLRPSHIDAYHDPITQTWLKIYSAYQE
HHHHHHHHHHCCCCHHHCCCHHCCEEECCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHH
ACDRAGLVDFAEILLRSHELLRDKKHIREHYQARFKHILVDEFQDTNNIQYAWLRMMAGP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
DCRVMIVGDDDQSIYGWRGAKIENIQKFLDEFPGASTVRLEQNYRSTKTILQASNELISN
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCC
NTERMGKELWTDGNDGEPISVYSAYNELDEARFTVSKIKEWQEKGGALEDTAMLYRNNAQ
CHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
SRVLEEALIQGGLPYRIYGGMRFFERQEIRDALSYLRLMSNRSNDAAFERVVNTPTRGLG
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DKTLETIRLAARDRGATMWEASVALIEEQVLPGRAAGALSRFIELINALEDDTIELRLHE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
QTDHVIKSSGLFAMYEQEKGEKSKARIENLEELVTATRQFEKPEEADEMSMLTAFLTHAA
HHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LEAGEGQADEFDDAVQLMTLHSAKGLEFPMVFMVGVEEGMFPSQMSAEEAGRLEEERRLC
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCHHHHHHHH
YVGMTRAMEKLYITYAEMRRLYGQDKYHKPSRFIRELPETCLDEVRMKAQVSRPASSGRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SQTAVKENFNETGFSLGSRVKHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYAR
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
LEQL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]