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The map label for this gene is dapF

Identifier: 218710944

GI number: 218710944

Start: 3194332

End: 3195162

Strand: Reverse

Name: dapF

Synonym: VS_3047

Alternate gene names: 218710944

Gene position: 3195162-3194332 (Counterclockwise)

Preceding gene: 218710945

Following gene: 218710943

Centisome position: 96.84

GC content: 46.57

Gene sequence:

>831_bases
ATGCACTTCCACTTTTCTAAAATGCATGGTTTGGGCAATGATTTCATGGTCGTGGACTGCATTACTCAAAATATTTTCTT
CTCTCCAGATTTGATCCGTCGTTTGGCGGATCGTCACACTGGTGTGGGTTTTGACCAGCTACTTGTAGTTGAAGCTCCCT
ATGATCCTGAAACTGACTTCCATTATCGCATATTTAATGCAGATGGCAGTGAAGTTGAGCAGTGTGGCAATGGTGCACGT
TGTTTTGCTCGATTCGTTCGCATGAAAGGCTTAACGAATAAGTACAGCATCAACGTGAGCACCAAGAAAGGGAAAATGGT
TCTTAAGATTGAAGACAATGATTTAATCACTGTCAACATGGGTATTCCTGAGTTCGAACCGGGCAAGATTCCATTTAAGG
CGAAGCAGCCAGAAAAGACGTATATCCTAAGAACGGATGTACACACCTTGTTCTGTGGTGCTGTTAGCATGGGTAACCCA
CATGTGGTTACCGTAGTTGATGACGTCGATACAGCAGATGTGGATACCTTAGGTCCGCTGCTTGAATCTCACGAACGTTT
TCCTGAGCGTGTTAATGCTGGCTTTATGCAAGTGGTTAACCGTGAAGAGGTTCGCTTGCGCGTCTACGAACGTGGTGCGG
GTGAAACTCAGGCGTGTGGCAGTGGCGCATGTGGTGCAGTTGCTGTTGGTATAACACAAGGCTTACTGGCTGAGAATGTG
AAGGTTCGTCTACCTGGTGGTGACTTACACATTAGCTGGCAAGGCCCTGGGAAACCTCTGTTGATGACTGGCCCAGCAAC
GCATGTGTTTGATGGTCAGCTTTCTTGCTAA

Upstream 100 bases:

>100_bases
TGATGGTTGATGGCGATAAAACTCATTTGGTTCGTCAGCGTGAAGAGCTTACGAGTCTGTGGGAACTTGAAAACATTCTT
CCGGAGTAATTTTAAGCGTT

Downstream 100 bases:

>100_bases
TAACGATATAACAAATAGATTTATAAAAAATAAAGGATAGGTTTTGTCTTACGTTGAAGGCGACACTCTGACTGCAGAGG
TGGTTGCGGAATATTTACGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHFHFSKMHGLGNDFMVVDCITQNIFFSPDLIRRLADRHTGVGFDQLLVVEAPYDPETDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYSINVSTKKGKMVLKIEDNDLITVNMGIPEFEPGKIPFKAKQPEKTYILRTDVHTLFCGAVSMGNP
HVVTVVDDVDTADVDTLGPLLESHERFPERVNAGFMQVVNREEVRLRVYERGAGETQACGSGACGAVAVGITQGLLAENV
KVRLPGGDLHISWQGPGKPLLMTGPATHVFDGQLSC

Sequences:

>Translated_276_residues
MHFHFSKMHGLGNDFMVVDCITQNIFFSPDLIRRLADRHTGVGFDQLLVVEAPYDPETDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYSINVSTKKGKMVLKIEDNDLITVNMGIPEFEPGKIPFKAKQPEKTYILRTDVHTLFCGAVSMGNP
HVVTVVDDVDTADVDTLGPLLESHERFPERVNAGFMQVVNREEVRLRVYERGAGETQACGSGACGAVAVGITQGLLAENV
KVRLPGGDLHISWQGPGKPLLMTGPATHVFDGQLSC
>Mature_276_residues
MHFHFSKMHGLGNDFMVVDCITQNIFFSPDLIRRLADRHTGVGFDQLLVVEAPYDPETDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYSINVSTKKGKMVLKIEDNDLITVNMGIPEFEPGKIPFKAKQPEKTYILRTDVHTLFCGAVSMGNP
HVVTVVDDVDTADVDTLGPLLESHERFPERVNAGFMQVVNREEVRLRVYERGAGETQACGSGACGAVAVGITQGLLAENV
KVRLPGGDLHISWQGPGKPLLMTGPATHVFDGQLSC

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=270, Percent_Identity=76.2962962962963, Blast_Score=439, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_VIBSL (B7VMD4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418565.1
- ProteinModelPortal:   B7VMD4
- SMR:   B7VMD4
- GeneID:   7162511
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_3047
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30411; Mature: 30411

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHFSKMHGLGNDFMVVDCITQNIFFSPDLIRRLADRHTGVGFDQLLVVEAPYDPETDF
CCEECHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYSINVSTKKGKMVLKIEDNDLITVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCEEEEEC
GIPEFEPGKIPFKAKQPEKTYILRTDVHTLFCGAVSMGNPHVVTVVDDVDTADVDTLGPL
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHH
LESHERFPERVNAGFMQVVNREEVRLRVYERGAGETQACGSGACGAVAVGITQGLLAENV
HHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCC
KVRLPGGDLHISWQGPGKPLLMTGPATHVFDGQLSC
EEEECCCCEEEEECCCCCCEEEECCCCEEECCCCCC
>Mature Secondary Structure
MHFHFSKMHGLGNDFMVVDCITQNIFFSPDLIRRLADRHTGVGFDQLLVVEAPYDPETDF
CCEECHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYSINVSTKKGKMVLKIEDNDLITVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCEEEEEC
GIPEFEPGKIPFKAKQPEKTYILRTDVHTLFCGAVSMGNPHVVTVVDDVDTADVDTLGPL
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHH
LESHERFPERVNAGFMQVVNREEVRLRVYERGAGETQACGSGACGAVAVGITQGLLAENV
HHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCC
KVRLPGGDLHISWQGPGKPLLMTGPATHVFDGQLSC
EEEECCCCEEEEECCCCCCEEEECCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA