Click here to switch to the map view.

The map label for this gene is yfgF [H]

Identifier: 218710939

GI number: 218710939

Start: 3186621

End: 3189107

Strand: Reverse

Name: yfgF [H]

Synonym: VS_3042

Alternate gene names: 218710939

Gene position: 3189107-3186621 (Counterclockwise)

Preceding gene: 218710940

Following gene: 218710936

Centisome position: 96.66

GC content: 45.4

Gene sequence:

>2487_bases
GTGAATATGCAATCGACCTCTTTCCAGATACAAGACATTAAGTGCACCCAGCGTGAATTGCAAAAGATAGATTTTCATCA
ACATGACAATCTACTGATTCAGGTGTTTTCTTCTTTCGAGAAAGATTCGGTGTTGGCTGCCTGCCAAGTAATTCAGTCGA
CTTTCCCCAACGCCAAATTGATTGGTTGTAGCGCTAATCACTACATCAGCCAAGGTGTGATCCTGCATAAAGGTCTCTAT
GTTATTGTTACTCGCTTTGAGGCGACGTCTTACACTTGTGGTGTTGTCGAATATAGCGAGCAGCCTCGACGGGATAGCCA
AGCGATGTGGCAGCAGCTTGAATGTAATGCTGATACTCAAAGCATTATCTGTTTCGCCGATCGCTTGCAGATAAATAACC
AAGATCTATTTTCAGCGTTTGAGCAGTCGATATATTCATTACCTGTTTGTGGCGGTGCATCGACCATTACAGAGCATGGA
CGATGGGTGATGCTCGATGGTATTTGCTATGAAAATGCCTATGTCATGTTAGCACTGCACAGTGTTGAACTCGCAATTAC
TAAGGGGTATTACTGCGAATGGAACCCCATTGGTCGTACCTTCCGAGTGACTGGCGCTCAAGATAATCGGCTAGCTGAAT
TGGACGGTATACCTGTCCGAGAAGTCTATAATCGTTACCTCGCTGACGGTCTCGAAATCCCTTTTGAGCAACTGTATAAC
TTCCCCTTGATGGTCGGTGAACCAAAAGACCAGAACACTCACTTACCGCTCAAGGTGACAGAGGGCGGAGAGATAGAATT
CAGTGGGTCGTTTCAGGTTGGTGATGAGGTGCGGTTTTGTTATGACCACCCTTCATTGACCCTAGAACAAATCCGACTGG
GCGTTCAACAGATTACTTCCCATAAACCAGAACAGTTTTTTATCTATAACTGCACTTCGCGCATTGATTTCATTGATGGC
AACCAAGAGGTTGAGGTTTTCCAAGCATTAGCGGACACCTATGGCGTGTACTGCATGGGAGAGCTCTATCAAGATAATGA
CCAGCAACGAATTTTGCATCATAGCCTCACTTATCTTGCGCTTAGGGAAGGTGAAGGCAAGGCGGTATTGCCATTAGAGA
CACAACCCGCGACCAATATTTCCCCTCTGTTTTCATTGATACGAAATGCTCTGCTTGATGTCGATGACATGAACAACAGC
ATGGCGAGTAAGATTCAACAGCAAGCGACCGCATTGACCGCGAGCTACCGAATTGATCGCCGTACAGGTTTACCTAACCG
AAGCGTACTGCGTGAAAAACTGTCAAAAATGAGTACCGATAGTCATTTGTTGGCTTTGAAAGTCACGACATTTGGCCAAA
TCAATGAGAAGTATGGCTATCGAGTCGGTGACAAGCTTCTGCATGATTTAAGCGAGTATTTTCAAAAGGCGCTGTGGCAG
TTCTTTGATGAGGGGTGTCAGCTCTATAGCATTGGTATCGGTGAGTGGGCGGTGGTGTTTGATAGCTGGGTGGGGCAAGA
ACATATTCATCAGCGTTTTTCTGAATTTGCAGACCAAACGGAACATGTGAATTTTGAACCAACGGGGTTGCCTGATATTG
ATTATCTGTCGGTATCCATCTGTGCAGGCATAGCCAGCCGTCGAGATTTCCCTACGACCTCGATTGATGATTTGTTGTTG
AAGGCGATTGATGCTAGGCGCAGCGCCGTGAGTCAGAACAAGCATCTAGTGAGCGCCAAGACGCTCATTCAATTGGAGAA
GCATCGCCAAGAGCAGCTTGGTTGGCTGTCGTGTGTCAGTCGCGCGGTGCTTAATCAGAATATTGTGGCGTGTTCCCAAC
TTATTGTCTCGGCACACAGCCATCAGGTAGAAAGCTATGAGTGCTTGGTTCGAATTGAAGAGGACGGTCAGTTGATTGCG
CCGGGTAAGTTCTTGCCAATCATAGAAGGAACTCATCTCTATACGCGTCTGAGTCGCCAGATGATAACGCGTACCTTTGA
TTTTATGAGTCAGCGAACCGATTCATTCTCAATTAACTTAGCGCCTCAAGATTTTAGCAATGAGAAAACCATTCTTCATC
TTGAGCAAGCCATCAAGAAAATCAGTCATCCACAGCGTATTGGTTTAGAGGTGTTGGAAACCGAACAAATTCAGGATTAC
GGTCGCTTGATCGAAGTGTGTAATCACTTCCGCGACCTTGGTGTGAATATTATTGTTGATGACTTTGGTTCCGGCTATTC
GAACATTGATGAGATATTGAAGTTAGAACCACAAGTCATAAAGCTTGATGGCAGTTTAATTCGGAATATTGATAAAGATA
AGAAACAGCGCAAGATCGCTCAGCAACTGGTGAGTTTGTGCCAGATACTGAACGCTAAAACAGTTGCGGAGTTCGTACAT
AATGAACAGGTGTGTCGAATCGCAGAGGATATGGGTGTCGATTACCTGCAAGGTTACTACTTTGGTCAGCCTCAACGCTT
ATTCTGA

Upstream 100 bases:

>100_bases
GTCTAACTAGCGTCACATTATTTATAAGACGATTGTAAGTATTCCGTGCAAGCTTTATCTATAATAAGCACAATAACAAA
TCATTAACGTAACGATTAAT

Downstream 100 bases:

>100_bases
TAGGGAGAGTAAAATCTCTTTTTAACGAACAGCTAGAGTGTGCGTTTCACGTGAAACACTAAATGGTTCAATAGATTAAA
GTATTATGTAAAAAGGGTGA

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 828; Mature: 828

Protein sequence:

>828_residues
MNMQSTSFQIQDIKCTQRELQKIDFHQHDNLLIQVFSSFEKDSVLAACQVIQSTFPNAKLIGCSANHYISQGVILHKGLY
VIVTRFEATSYTCGVVEYSEQPRRDSQAMWQQLECNADTQSIICFADRLQINNQDLFSAFEQSIYSLPVCGGASTITEHG
RWVMLDGICYENAYVMLALHSVELAITKGYYCEWNPIGRTFRVTGAQDNRLAELDGIPVREVYNRYLADGLEIPFEQLYN
FPLMVGEPKDQNTHLPLKVTEGGEIEFSGSFQVGDEVRFCYDHPSLTLEQIRLGVQQITSHKPEQFFIYNCTSRIDFIDG
NQEVEVFQALADTYGVYCMGELYQDNDQQRILHHSLTYLALREGEGKAVLPLETQPATNISPLFSLIRNALLDVDDMNNS
MASKIQQQATALTASYRIDRRTGLPNRSVLREKLSKMSTDSHLLALKVTTFGQINEKYGYRVGDKLLHDLSEYFQKALWQ
FFDEGCQLYSIGIGEWAVVFDSWVGQEHIHQRFSEFADQTEHVNFEPTGLPDIDYLSVSICAGIASRRDFPTTSIDDLLL
KAIDARRSAVSQNKHLVSAKTLIQLEKHRQEQLGWLSCVSRAVLNQNIVACSQLIVSAHSHQVESYECLVRIEEDGQLIA
PGKFLPIIEGTHLYTRLSRQMITRTFDFMSQRTDSFSINLAPQDFSNEKTILHLEQAIKKISHPQRIGLEVLETEQIQDY
GRLIEVCNHFRDLGVNIIVDDFGSGYSNIDEILKLEPQVIKLDGSLIRNIDKDKKQRKIAQQLVSLCQILNAKTVAEFVH
NEQVCRIAEDMGVDYLQGYYFGQPQRLF

Sequences:

>Translated_828_residues
MNMQSTSFQIQDIKCTQRELQKIDFHQHDNLLIQVFSSFEKDSVLAACQVIQSTFPNAKLIGCSANHYISQGVILHKGLY
VIVTRFEATSYTCGVVEYSEQPRRDSQAMWQQLECNADTQSIICFADRLQINNQDLFSAFEQSIYSLPVCGGASTITEHG
RWVMLDGICYENAYVMLALHSVELAITKGYYCEWNPIGRTFRVTGAQDNRLAELDGIPVREVYNRYLADGLEIPFEQLYN
FPLMVGEPKDQNTHLPLKVTEGGEIEFSGSFQVGDEVRFCYDHPSLTLEQIRLGVQQITSHKPEQFFIYNCTSRIDFIDG
NQEVEVFQALADTYGVYCMGELYQDNDQQRILHHSLTYLALREGEGKAVLPLETQPATNISPLFSLIRNALLDVDDMNNS
MASKIQQQATALTASYRIDRRTGLPNRSVLREKLSKMSTDSHLLALKVTTFGQINEKYGYRVGDKLLHDLSEYFQKALWQ
FFDEGCQLYSIGIGEWAVVFDSWVGQEHIHQRFSEFADQTEHVNFEPTGLPDIDYLSVSICAGIASRRDFPTTSIDDLLL
KAIDARRSAVSQNKHLVSAKTLIQLEKHRQEQLGWLSCVSRAVLNQNIVACSQLIVSAHSHQVESYECLVRIEEDGQLIA
PGKFLPIIEGTHLYTRLSRQMITRTFDFMSQRTDSFSINLAPQDFSNEKTILHLEQAIKKISHPQRIGLEVLETEQIQDY
GRLIEVCNHFRDLGVNIIVDDFGSGYSNIDEILKLEPQVIKLDGSLIRNIDKDKKQRKIAQQLVSLCQILNAKTVAEFVH
NEQVCRIAEDMGVDYLQGYYFGQPQRLF
>Mature_828_residues
MNMQSTSFQIQDIKCTQRELQKIDFHQHDNLLIQVFSSFEKDSVLAACQVIQSTFPNAKLIGCSANHYISQGVILHKGLY
VIVTRFEATSYTCGVVEYSEQPRRDSQAMWQQLECNADTQSIICFADRLQINNQDLFSAFEQSIYSLPVCGGASTITEHG
RWVMLDGICYENAYVMLALHSVELAITKGYYCEWNPIGRTFRVTGAQDNRLAELDGIPVREVYNRYLADGLEIPFEQLYN
FPLMVGEPKDQNTHLPLKVTEGGEIEFSGSFQVGDEVRFCYDHPSLTLEQIRLGVQQITSHKPEQFFIYNCTSRIDFIDG
NQEVEVFQALADTYGVYCMGELYQDNDQQRILHHSLTYLALREGEGKAVLPLETQPATNISPLFSLIRNALLDVDDMNNS
MASKIQQQATALTASYRIDRRTGLPNRSVLREKLSKMSTDSHLLALKVTTFGQINEKYGYRVGDKLLHDLSEYFQKALWQ
FFDEGCQLYSIGIGEWAVVFDSWVGQEHIHQRFSEFADQTEHVNFEPTGLPDIDYLSVSICAGIASRRDFPTTSIDDLLL
KAIDARRSAVSQNKHLVSAKTLIQLEKHRQEQLGWLSCVSRAVLNQNIVACSQLIVSAHSHQVESYECLVRIEEDGQLIA
PGKFLPIIEGTHLYTRLSRQMITRTFDFMSQRTDSFSINLAPQDFSNEKTILHLEQAIKKISHPQRIGLEVLETEQIQDY
GRLIEVCNHFRDLGVNIIVDDFGSGYSNIDEILKLEPQVIKLDGSLIRNIDKDKKQRKIAQQLVSLCQILNAKTVAEFVH
NEQVCRIAEDMGVDYLQGYYFGQPQRLF

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=454, Percent_Identity=23.1277533039648, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1790496, Length=249, Percent_Identity=28.1124497991968, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI87082096, Length=211, Percent_Identity=28.9099526066351, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1787541, Length=423, Percent_Identity=23.4042553191489, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI87081980, Length=221, Percent_Identity=31.2217194570136, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081921, Length=485, Percent_Identity=20.6185567010309, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI87081743, Length=229, Percent_Identity=26.6375545851528, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081845, Length=262, Percent_Identity=24.0458015267176, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788502, Length=238, Percent_Identity=23.109243697479, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787410, Length=234, Percent_Identity=24.7863247863248, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1786507, Length=238, Percent_Identity=24.7899159663866, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 94325; Mature: 94325

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMQSTSFQIQDIKCTQRELQKIDFHQHDNLLIQVFSSFEKDSVLAACQVIQSTFPNAKL
CCCCCCCEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
IGCSANHYISQGVILHKGLYVIVTRFEATSYTCGVVEYSEQPRRDSQAMWQQLECNADTQ
EECCCCHHHHCCCCEECCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCC
SIICFADRLQINNQDLFSAFEQSIYSLPVCGGASTITEHGRWVMLDGICYENAYVMLALH
EEEEEEEHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCEEECCCEEEEEEE
SVELAITKGYYCEWNPIGRTFRVTGAQDNRLAELDGIPVREVYNRYLADGLEIPFEQLYN
EEEEEEECCEEEEECCCCCEEEEECCCCCCCHHCCCCCHHHHHHHHHHCCCCCCHHHHCC
FPLMVGEPKDQNTHLPLKVTEGGEIEFSGSFQVGDEVRFCYDHPSLTLEQIRLGVQQITS
CCEEECCCCCCCCCCCEEECCCCEEEEECCEEECCCEEEEECCCCCCHHHHHHHHHHHHC
HKPEQFFIYNCTSRIDFIDGNQEVEVFQALADTYGVYCMGELYQDNDQQRILHHSLTYLA
CCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHCCEEEEEHHHCCCCHHHHHHHHHHEEE
LREGEGKAVLPLETQPATNISPLFSLIRNALLDVDDMNNSMASKIQQQATALTASYRIDR
EECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHEEEHH
RTGLPNRSVLREKLSKMSTDSHLLALKVTTFGQINEKYGYRVGDKLLHDLSEYFQKALWQ
HCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
FFDEGCQLYSIGIGEWAVVFDSWVGQEHIHQRFSEFADQTEHVNFEPTGLPDIDYLSVSI
HHHCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
CAGIASRRDFPTTSIDDLLLKAIDARRSAVSQNKHLVSAKTLIQLEKHRQEQLGWLSCVS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVLNQNIVACSQLIVSAHSHQVESYECLVRIEEDGQLIAPGKFLPIIEGTHLYTRLSRQ
HHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCEEEEECCCHHHHHHHHH
MITRTFDFMSQRTDSFSINLAPQDFSNEKTILHLEQAIKKISHPQRIGLEVLETEQIQDY
HHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
GRLIEVCNHFRDLGVNIIVDDFGSGYSNIDEILKLEPQVIKLDGSLIRNIDKDKKQRKIA
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHH
QQLVSLCQILNAKTVAEFVHNEQVCRIAEDMGVDYLQGYYFGQPQRLF
HHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCHHHHCEECCCCCCCC
>Mature Secondary Structure
MNMQSTSFQIQDIKCTQRELQKIDFHQHDNLLIQVFSSFEKDSVLAACQVIQSTFPNAKL
CCCCCCCEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
IGCSANHYISQGVILHKGLYVIVTRFEATSYTCGVVEYSEQPRRDSQAMWQQLECNADTQ
EECCCCHHHHCCCCEECCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCC
SIICFADRLQINNQDLFSAFEQSIYSLPVCGGASTITEHGRWVMLDGICYENAYVMLALH
EEEEEEEHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCEEECCCEEEEEEE
SVELAITKGYYCEWNPIGRTFRVTGAQDNRLAELDGIPVREVYNRYLADGLEIPFEQLYN
EEEEEEECCEEEEECCCCCEEEEECCCCCCCHHCCCCCHHHHHHHHHHCCCCCCHHHHCC
FPLMVGEPKDQNTHLPLKVTEGGEIEFSGSFQVGDEVRFCYDHPSLTLEQIRLGVQQITS
CCEEECCCCCCCCCCCEEECCCCEEEEECCEEECCCEEEEECCCCCCHHHHHHHHHHHHC
HKPEQFFIYNCTSRIDFIDGNQEVEVFQALADTYGVYCMGELYQDNDQQRILHHSLTYLA
CCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHCCEEEEEHHHCCCCHHHHHHHHHHEEE
LREGEGKAVLPLETQPATNISPLFSLIRNALLDVDDMNNSMASKIQQQATALTASYRIDR
EECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHEEEHH
RTGLPNRSVLREKLSKMSTDSHLLALKVTTFGQINEKYGYRVGDKLLHDLSEYFQKALWQ
HCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
FFDEGCQLYSIGIGEWAVVFDSWVGQEHIHQRFSEFADQTEHVNFEPTGLPDIDYLSVSI
HHHCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
CAGIASRRDFPTTSIDDLLLKAIDARRSAVSQNKHLVSAKTLIQLEKHRQEQLGWLSCVS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVLNQNIVACSQLIVSAHSHQVESYECLVRIEEDGQLIAPGKFLPIIEGTHLYTRLSRQ
HHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCCEEEEECCCHHHHHHHHH
MITRTFDFMSQRTDSFSINLAPQDFSNEKTILHLEQAIKKISHPQRIGLEVLETEQIQDY
HHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
GRLIEVCNHFRDLGVNIIVDDFGSGYSNIDEILKLEPQVIKLDGSLIRNIDKDKKQRKIA
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHH
QQLVSLCQILNAKTVAEFVHNEQVCRIAEDMGVDYLQGYYFGQPQRLF
HHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCHHHHCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]