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The map label for this gene is yfgF [C]

Identifier: 218710940

GI number: 218710940

Start: 3189293

End: 3191845

Strand: Reverse

Name: yfgF [C]

Synonym: VS_3043

Alternate gene names: 218710940

Gene position: 3191845-3189293 (Counterclockwise)

Preceding gene: 218710941

Following gene: 218710939

Centisome position: 96.74

GC content: 44.73

Gene sequence:

>2553_bases
TTGATTCATAGTAGTGCGGGTTCTCATTTCACTCGATTTCGAACAAGGGTTGGCATGCAAACATTTACATTTCTCGCAAA
TACTGAGGAGTTATTTAAGTCTCAATTTGATCAGCGAGAGTGGTGTGACAGCAAACAGTACCTTATTCAAATTTTCTCTG
CTCAGTCCTCTGATATCGCTCGTCGAATTGCCAATGCAGCCCTCAATCGCATAGGGCATGCGACGTTAATCGGTCAGAGC
GCCCGTTATGTTATCTGTGATAACAGCCTGAAGCATGAATGTACCTTAGTCGTCGTCAGTGAATTTAACGATACCCATTT
AACGTCTACAGTTCAGACGTTTACCGGAAACCCAAATCAAGACAGCCAAGATCTCGCTTCTCAATTAAACCTTACAGAAG
AAACCAAAACGGTTATCAGCTTGTGCGATCAGGTTGAAGGGCGTGACTATCCTATTTATAGCGCCTTTGAGAGTTTACCT
TACGCATTACCAGTTGCTGGTGGGCTGTGCCACGAGAATGAATATGGCCGTTGGGTTATGCATAATGAGCAAACCTACCA
ACATGCTTGTGTTGCGGTTGCATTGACTAACCCTAGCTTAAAGATTTGGTCGGACGCGTATTCTGAGTGGAATCCTATTG
GGATGAAATTGCGAGTGACACACGCAATTGGAAATCGTTTATATGCGTTGAATGACAAGCCGGCTATCGAGGTGTTCAAA
CATTACCTTGCTGATGGTAAAGATCTCCCTTTTAGTCAGTTGATGAGCTTCCCCCTGTATCGCGAGCTCGGCAGAAAGAA
GTGCATCTCGACACCTCTACGTATTAATGATGATGGTAGTATTGAGTTTGATAGCCCTTGGCATGTCGGCGAAGAGGCCC
AGTTTTGTTATAACCATCCTTCTTTGACCGCAGAAAAAGTGCGTCACGGCGCTGAGATGCTTGCCGTGCATCAGCCTGAA
TCGGTGATCATCTATAACTGTGTATCTCGACTAGAGTTTATCGACAGTAAACTTGAGCTCAAACCATTTGAAGGGATAGT
AAACGCGTGTGGTGCATACTGCATGGGCGAACTATATCGCAACGACGATCGCCAAGAGATACTGCATCACAGCCTTACCT
ATATTGCGATGAGAGAGTCGGACGAGATTAATGAGTTTCGTTGCGAAGATTTCAAGCGAGACTCAACGGTATCGCCACTA
CTCAACTTAGTCAGAAATGCGGTCGCAGACCTCGACAGTATGAACACTCAGATGGAGAACAAACTCCATCAACAGGCACG
TCGCTTAACTGAAAGCTACCGTATTGACTCTCGCACAGGGCTACCCAACCGTATTGTATTAAAAGAACGCCTCAATACGA
TTTTATTCAATGAACATCTGTTGACGCTGAAGCTGACTAATTTTCATCAAGTTAACGAAAAATACGGTTATCAAGTGGGT
GACCAATTACTGCTTGATCTGTCGAATCATTTTGTCGACCGATTGCACCTTAGTGTGGCTAAGGAATCCAAGGTAAAGGT
TGAACTGTTCAGTATTGGTGTCGGTGAGTGGGCAATTATCTTTAACGCGAGTGTCGATAGCGTGAAGATAGAGCAACGTT
TCGTCGAGTTTGCTGATGGTATTGAGCATATTAATTTTGAGCCATATGGGTTAACCGACATAGACTACCTTTCGGTTTCT
CTGTGTGGTGGTTTCGCTAGTCGCTGTGATTTCTTAGCTGATAGCGGTGATGAGATCTTGTTGAAAGCGATTGAAGCTCG
ACGCTACGGGGTGCGGAACAATACTCACATCACTAATGCCAAAAACATTCAGGTGAGTGAGGAAGATCGTAAAGAACAAC
TAGGCTGGTTGAGCTGCGTAAGCCGCGCAATCTTAGATCAGAACATTATCACCTACTCACAGCCGATTGTGGCGTCTGGT
ACTCACGAAATGATTGGCCAAGAGTGCTTGGTCAGAATCATGGAGTCGGATGGTACCATTGTACTCCCTGGTAAGTTCTT
ACCCATTATTGCTGACACGCATCTCTATACTCGCCTTAGCCGGCACATGATTCGAAACACCATTGGCTACATGGCAAATA
AGGAGAGTTCTTTTTCAATCAACCTATCCCCACAAGACTTACTGAGCGATAAGACGCTTGAGGTTCTCGAGTCAGCGATT
AGTGGTATGAACGACCCCACTCGACTTGGTCTAGAGGTTCTCGAGTCTGAACAGATCAAAGATTATGGCCGCATGATAGA
AGTGTGTGATCACTTCCGTGCTCTTGGGGCGAGAATCATTGTTGATGACTTTGGTTCTGGTTATTCCAATATTGATGAGA
TCATTAAACTTGAGCCGCAGATCATTAAGCTCGACGGTAGCTTGATTCGCAATATCGACAAAGACCCAAAGCAAAGGAAT
ATCGCCTCTCAATTAGTACGCTTGTGTCAGGTGTTTAACGCCAAAACAGTTGCCGAATTTGTCCATAACCAACAGGTTTG
TGAAATTGCAGAGCAAATGGGTGTCGATTATCTGCAAGGTTATTACTTTGGTGAGCCTAAGCGACTGTTTTAG

Upstream 100 bases:

>100_bases
AGCTTAATGTGTCGCATTTATGATATTGAATTGTTACATTAGTCACAATTAAATGCCTGTTTAGGCTAGGATAATTGGCG
ACTACTTTTGATCGAAATTT

Downstream 100 bases:

>100_bases
TTATTAAAACCCTCAGTTCTTAACGTTACTTGCTTTTAAAGTGCTTCACTGGCACTGATGATGGCTCTTTAGTGGAATGA
ATGATGTCTAACTAGCGTCA

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 850; Mature: 850

Protein sequence:

>850_residues
MIHSSAGSHFTRFRTRVGMQTFTFLANTEELFKSQFDQREWCDSKQYLIQIFSAQSSDIARRIANAALNRIGHATLIGQS
ARYVICDNSLKHECTLVVVSEFNDTHLTSTVQTFTGNPNQDSQDLASQLNLTEETKTVISLCDQVEGRDYPIYSAFESLP
YALPVAGGLCHENEYGRWVMHNEQTYQHACVAVALTNPSLKIWSDAYSEWNPIGMKLRVTHAIGNRLYALNDKPAIEVFK
HYLADGKDLPFSQLMSFPLYRELGRKKCISTPLRINDDGSIEFDSPWHVGEEAQFCYNHPSLTAEKVRHGAEMLAVHQPE
SVIIYNCVSRLEFIDSKLELKPFEGIVNACGAYCMGELYRNDDRQEILHHSLTYIAMRESDEINEFRCEDFKRDSTVSPL
LNLVRNAVADLDSMNTQMENKLHQQARRLTESYRIDSRTGLPNRIVLKERLNTILFNEHLLTLKLTNFHQVNEKYGYQVG
DQLLLDLSNHFVDRLHLSVAKESKVKVELFSIGVGEWAIIFNASVDSVKIEQRFVEFADGIEHINFEPYGLTDIDYLSVS
LCGGFASRCDFLADSGDEILLKAIEARRYGVRNNTHITNAKNIQVSEEDRKEQLGWLSCVSRAILDQNIITYSQPIVASG
THEMIGQECLVRIMESDGTIVLPGKFLPIIADTHLYTRLSRHMIRNTIGYMANKESSFSINLSPQDLLSDKTLEVLESAI
SGMNDPTRLGLEVLESEQIKDYGRMIEVCDHFRALGARIIVDDFGSGYSNIDEIIKLEPQIIKLDGSLIRNIDKDPKQRN
IASQLVRLCQVFNAKTVAEFVHNQQVCEIAEQMGVDYLQGYYFGEPKRLF

Sequences:

>Translated_850_residues
MIHSSAGSHFTRFRTRVGMQTFTFLANTEELFKSQFDQREWCDSKQYLIQIFSAQSSDIARRIANAALNRIGHATLIGQS
ARYVICDNSLKHECTLVVVSEFNDTHLTSTVQTFTGNPNQDSQDLASQLNLTEETKTVISLCDQVEGRDYPIYSAFESLP
YALPVAGGLCHENEYGRWVMHNEQTYQHACVAVALTNPSLKIWSDAYSEWNPIGMKLRVTHAIGNRLYALNDKPAIEVFK
HYLADGKDLPFSQLMSFPLYRELGRKKCISTPLRINDDGSIEFDSPWHVGEEAQFCYNHPSLTAEKVRHGAEMLAVHQPE
SVIIYNCVSRLEFIDSKLELKPFEGIVNACGAYCMGELYRNDDRQEILHHSLTYIAMRESDEINEFRCEDFKRDSTVSPL
LNLVRNAVADLDSMNTQMENKLHQQARRLTESYRIDSRTGLPNRIVLKERLNTILFNEHLLTLKLTNFHQVNEKYGYQVG
DQLLLDLSNHFVDRLHLSVAKESKVKVELFSIGVGEWAIIFNASVDSVKIEQRFVEFADGIEHINFEPYGLTDIDYLSVS
LCGGFASRCDFLADSGDEILLKAIEARRYGVRNNTHITNAKNIQVSEEDRKEQLGWLSCVSRAILDQNIITYSQPIVASG
THEMIGQECLVRIMESDGTIVLPGKFLPIIADTHLYTRLSRHMIRNTIGYMANKESSFSINLSPQDLLSDKTLEVLESAI
SGMNDPTRLGLEVLESEQIKDYGRMIEVCDHFRALGARIIVDDFGSGYSNIDEIIKLEPQIIKLDGSLIRNIDKDPKQRN
IASQLVRLCQVFNAKTVAEFVHNQQVCEIAEQMGVDYLQGYYFGEPKRLF
>Mature_850_residues
MIHSSAGSHFTRFRTRVGMQTFTFLANTEELFKSQFDQREWCDSKQYLIQIFSAQSSDIARRIANAALNRIGHATLIGQS
ARYVICDNSLKHECTLVVVSEFNDTHLTSTVQTFTGNPNQDSQDLASQLNLTEETKTVISLCDQVEGRDYPIYSAFESLP
YALPVAGGLCHENEYGRWVMHNEQTYQHACVAVALTNPSLKIWSDAYSEWNPIGMKLRVTHAIGNRLYALNDKPAIEVFK
HYLADGKDLPFSQLMSFPLYRELGRKKCISTPLRINDDGSIEFDSPWHVGEEAQFCYNHPSLTAEKVRHGAEMLAVHQPE
SVIIYNCVSRLEFIDSKLELKPFEGIVNACGAYCMGELYRNDDRQEILHHSLTYIAMRESDEINEFRCEDFKRDSTVSPL
LNLVRNAVADLDSMNTQMENKLHQQARRLTESYRIDSRTGLPNRIVLKERLNTILFNEHLLTLKLTNFHQVNEKYGYQVG
DQLLLDLSNHFVDRLHLSVAKESKVKVELFSIGVGEWAIIFNASVDSVKIEQRFVEFADGIEHINFEPYGLTDIDYLSVS
LCGGFASRCDFLADSGDEILLKAIEARRYGVRNNTHITNAKNIQVSEEDRKEQLGWLSCVSRAILDQNIITYSQPIVASG
THEMIGQECLVRIMESDGTIVLPGKFLPIIADTHLYTRLSRHMIRNTIGYMANKESSFSINLSPQDLLSDKTLEVLESAI
SGMNDPTRLGLEVLESEQIKDYGRMIEVCDHFRALGARIIVDDFGSGYSNIDEIIKLEPQIIKLDGSLIRNIDKDPKQRN
IASQLVRLCQVFNAKTVAEFVHNQQVCEIAEQMGVDYLQGYYFGEPKRLF

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MHYT domain [H]

Homologues:

Organism=Escherichia coli, GI87082096, Length=235, Percent_Identity=30.2127659574468, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788849, Length=249, Percent_Identity=26.5060240963855, Blast_Score=110, Evalue=5e-25,
Organism=Escherichia coli, GI1790496, Length=239, Percent_Identity=27.1966527196653, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI87081743, Length=236, Percent_Identity=28.3898305084746, Blast_Score=100, Evalue=7e-22,
Organism=Escherichia coli, GI87081921, Length=452, Percent_Identity=21.6814159292035, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787541, Length=239, Percent_Identity=25.9414225941423, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI87081980, Length=222, Percent_Identity=25.6756756756757, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI87081845, Length=233, Percent_Identity=26.6094420600858, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI226510982, Length=422, Percent_Identity=22.2748815165877, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1786507, Length=235, Percent_Identity=24.2553191489362, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1787055, Length=229, Percent_Identity=26.6375545851528, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1788502, Length=239, Percent_Identity=21.7573221757322, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 96637; Mature: 96637

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHSSAGSHFTRFRTRVGMQTFTFLANTEELFKSQFDQREWCDSKQYLIQIFSAQSSDIA
CCCCCCCCHHHHHHHHHCHHHHHHHCCHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHH
RRIANAALNRIGHATLIGQSARYVICDNSLKHECTLVVVSEFNDTHLTSTVQTFTGNPNQ
HHHHHHHHHHCCCCEEECCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCC
DSQDLASQLNLTEETKTVISLCDQVEGRDYPIYSAFESLPYALPVAGGLCHENEYGRWVM
CHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
HNEQTYQHACVAVALTNPSLKIWSDAYSEWNPIGMKLRVTHAIGNRLYALNDKPAIEVFK
ECCCHHHEEEEEEEEECCCCEEHHHHHCCCCCCCEEEEEEEHHCCEEEEECCCCHHHHHH
HYLADGKDLPFSQLMSFPLYRELGRKKCISTPLRINDDGSIEFDSPWHVGEEAQFCYNHP
HHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCCCCHHHCCCC
SLTAEKVRHGAEMLAVHQPESVIIYNCVSRLEFIDSKLELKPFEGIVNACGAYCMGELYR
CCCHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
NDDRQEILHHSLTYIAMRESDEINEFRCEDFKRDSTVSPLLNLVRNAVADLDSMNTQMEN
CCCHHHHHHHHHHEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KLHQQARRLTESYRIDSRTGLPNRIVLKERLNTILFNEHLLTLKLTNFHQVNEKYGYQVG
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCHHH
DQLLLDLSNHFVDRLHLSVAKESKVKVELFSIGVGEWAIIFNASVDSVKIEQRFVEFADG
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC
IEHINFEPYGLTDIDYLSVSLCGGFASRCDFLADSGDEILLKAIEARRYGVRNNTHITNA
HHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEECCC
KNIQVSEEDRKEQLGWLSCVSRAILDQNIITYSQPIVASGTHEMIGQECLVRIMESDGTI
CCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
VLPGKFLPIIADTHLYTRLSRHMIRNTIGYMANKESSFSINLSPQDLLSDKTLEVLESAI
EECCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCHHHHHHHHHHH
SGMNDPTRLGLEVLESEQIKDYGRMIEVCDHFRALGARIIVDDFGSGYSNIDEIIKLEPQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCE
IIKLDGSLIRNIDKDPKQRNIASQLVRLCQVFNAKTVAEFVHNQQVCEIAEQMGVDYLQG
EEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHCHHHHCC
YYFGEPKRLF
EEECCCCCCC
>Mature Secondary Structure
MIHSSAGSHFTRFRTRVGMQTFTFLANTEELFKSQFDQREWCDSKQYLIQIFSAQSSDIA
CCCCCCCCHHHHHHHHHCHHHHHHHCCHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHH
RRIANAALNRIGHATLIGQSARYVICDNSLKHECTLVVVSEFNDTHLTSTVQTFTGNPNQ
HHHHHHHHHHCCCCEEECCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCC
DSQDLASQLNLTEETKTVISLCDQVEGRDYPIYSAFESLPYALPVAGGLCHENEYGRWVM
CHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
HNEQTYQHACVAVALTNPSLKIWSDAYSEWNPIGMKLRVTHAIGNRLYALNDKPAIEVFK
ECCCHHHEEEEEEEEECCCCEEHHHHHCCCCCCCEEEEEEEHHCCEEEEECCCCHHHHHH
HYLADGKDLPFSQLMSFPLYRELGRKKCISTPLRINDDGSIEFDSPWHVGEEAQFCYNHP
HHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCCCCHHHCCCC
SLTAEKVRHGAEMLAVHQPESVIIYNCVSRLEFIDSKLELKPFEGIVNACGAYCMGELYR
CCCHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
NDDRQEILHHSLTYIAMRESDEINEFRCEDFKRDSTVSPLLNLVRNAVADLDSMNTQMEN
CCCHHHHHHHHHHEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KLHQQARRLTESYRIDSRTGLPNRIVLKERLNTILFNEHLLTLKLTNFHQVNEKYGYQVG
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCHHH
DQLLLDLSNHFVDRLHLSVAKESKVKVELFSIGVGEWAIIFNASVDSVKIEQRFVEFADG
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC
IEHINFEPYGLTDIDYLSVSLCGGFASRCDFLADSGDEILLKAIEARRYGVRNNTHITNA
HHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEECCC
KNIQVSEEDRKEQLGWLSCVSRAILDQNIITYSQPIVASGTHEMIGQECLVRIMESDGTI
CCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
VLPGKFLPIIADTHLYTRLSRHMIRNTIGYMANKESSFSINLSPQDLLSDKTLEVLESAI
EECCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCHHHHHHHHHHH
SGMNDPTRLGLEVLESEQIKDYGRMIEVCDHFRALGARIIVDDFGSGYSNIDEIIKLEPQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHCCCE
IIKLDGSLIRNIDKDPKQRNIASQLVRLCQVFNAKTVAEFVHNQQVCEIAEQMGVDYLQG
EEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHCHHHHCC
YYFGEPKRLF
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]