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The map label for this gene is gap3 [H]

Identifier: 218710930

GI number: 218710930

Start: 3178194

End: 3179195

Strand: Reverse

Name: gap3 [H]

Synonym: VS_3033

Alternate gene names: 218710930

Gene position: 3179195-3178194 (Counterclockwise)

Preceding gene: 218710931

Following gene: 218710929

Centisome position: 96.36

GC content: 48.2

Gene sequence:

>1002_bases
ATGACAGTCAAAGTAGGTATTAATGGTTTTGGCCGTATCGGCCGTTTAGCACTTCGCGCAGCGTTTGATTGGGCGGAAAT
TGAGTTCGTACAGATTAACGATGTGGCTGGCGACACCGCGACATTGGCTCACCTTCTTGAGTTCGATTCGGTTCAAGGTC
GCTGGAACCACGAAGTTGCGGTTGAAGGCGATGAGATGATCATCAACGGTCAACGCATTAGAACTACGCAAGAGCGCGAC
ATCGATGCGATCGATTGGTCTGGTTGCGATGTTGTCCTTGAAGCGACCGGTGTTCACCGTAAGGCATCTTTCCTAAATAA
ATACCTTGAGCAAGGCGTGAAGCGCGTTGTGGTATCGGCGCCAGTGAAAGAAGAAGGCATTGCAAACATCGTTGTCGGTG
TGAACGACAATATCTTCGATCCAGCAGTACATAAAATCGTAACCGCAGCATCTTGTACAACCAACTGTCTCGCGCCCGTG
GTTAAAGTGATCAACGAGAAGCTGGGTATCGAAAATGCAGCTTTCACTACTATTCACGATCTAACCAATACGCAAACTAT
TCTTGATGCGCCGCATAAAGATTTACGCCGTGCTCGTGCATGTGGCATGAGTCTTATTCCGACAACAACGGGCAGTGCTA
AAGCGATTGTTGAGATATTCCCAGAGCTTGAAAACCGCATCAATGGCCACGCAGTGCGTGTACCACTAGCGAACGCGTCT
CTGACCGACATCATCTTTGAAGTGAAGCAAGACACGACAGCAGAAGAAGTTAACGCGATGCTGAAAGAAGCGTCTGAGAA
CGAACTAAAAGGCATTCTTGGTTTTGAAGAGCGTCCACTGGTTTCTATCGATTACAAAGGCGACCAACGTTCAACCATCG
TAGATGCACTATCAACCATGTTAGTGGGTAAGCGCATGGTTAAGATCTACGCTTGGTACGACAACGAGATGGGTTACGCG
ACACGTACAGCAGAGCTAGTACGTACAGTTGGCCTAGCTTAA

Upstream 100 bases:

>100_bases
TACATTTAAATTTATTGCCAAAATCGTTGAGATTGCAGCTAGGCATAGTCAACAACACTGCGGCTTCAAATATGAAGGCA
ATTAACTGAGAGAAAAGAGT

Downstream 100 bases:

>100_bases
TACTCTTCATACTTGAAGCTGCAAGCGTTGTTAACTGCGTAAGTTCACCGGAGCGCCGGCCGCCCTAATCACATAGAACC
CCTATGCTCATAGGGCTGAA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTVKVGINGFGRIGRLALRAAFDWAEIEFVQINDVAGDTATLAHLLEFDSVQGRWNHEVAVEGDEMIINGQRIRTTQERD
IDAIDWSGCDVVLEATGVHRKASFLNKYLEQGVKRVVVSAPVKEEGIANIVVGVNDNIFDPAVHKIVTAASCTTNCLAPV
VKVINEKLGIENAAFTTIHDLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELENRINGHAVRVPLANAS
LTDIIFEVKQDTTAEEVNAMLKEASENELKGILGFEERPLVSIDYKGDQRSTIVDALSTMLVGKRMVKIYAWYDNEMGYA
TRTAELVRTVGLA

Sequences:

>Translated_333_residues
MTVKVGINGFGRIGRLALRAAFDWAEIEFVQINDVAGDTATLAHLLEFDSVQGRWNHEVAVEGDEMIINGQRIRTTQERD
IDAIDWSGCDVVLEATGVHRKASFLNKYLEQGVKRVVVSAPVKEEGIANIVVGVNDNIFDPAVHKIVTAASCTTNCLAPV
VKVINEKLGIENAAFTTIHDLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELENRINGHAVRVPLANAS
LTDIIFEVKQDTTAEEVNAMLKEASENELKGILGFEERPLVSIDYKGDQRSTIVDALSTMLVGKRMVKIYAWYDNEMGYA
TRTAELVRTVGLA
>Mature_332_residues
TVKVGINGFGRIGRLALRAAFDWAEIEFVQINDVAGDTATLAHLLEFDSVQGRWNHEVAVEGDEMIINGQRIRTTQERDI
DAIDWSGCDVVLEATGVHRKASFLNKYLEQGVKRVVVSAPVKEEGIANIVVGVNDNIFDPAVHKIVTAASCTTNCLAPVV
KVINEKLGIENAAFTTIHDLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELENRINGHAVRVPLANASL
TDIIFEVKQDTTAEEVNAMLKEASENELKGILGFEERPLVSIDYKGDQRSTIVDALSTMLVGKRMVKIYAWYDNEMGYAT
RTAELVRTVGLA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=328, Percent_Identity=40.2439024390244, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI7657116, Length=327, Percent_Identity=37.0030581039755, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1788079, Length=331, Percent_Identity=42.2960725075529, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1789295, Length=327, Percent_Identity=41.5902140672783, Blast_Score=254, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI32566163, Length=334, Percent_Identity=38.622754491018, Blast_Score=250, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17568413, Length=334, Percent_Identity=38.622754491018, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI17534677, Length=331, Percent_Identity=37.1601208459215, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17534679, Length=331, Percent_Identity=36.5558912386707, Blast_Score=237, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6321631, Length=334, Percent_Identity=41.0179640718563, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=40.4191616766467, Blast_Score=271, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6322409, Length=334, Percent_Identity=39.5209580838323, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI85725000, Length=328, Percent_Identity=40.5487804878049, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI22023983, Length=328, Percent_Identity=40.5487804878049, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17933600, Length=328, Percent_Identity=39.6341463414634, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI18110149, Length=328, Percent_Identity=39.6341463414634, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=37.4233128834356, Blast_Score=248, Evalue=4e-66,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36502; Mature: 36371

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKVGINGFGRIGRLALRAAFDWAEIEFVQINDVAGDTATLAHLLEFDSVQGRWNHEVA
CEEEEECCCCCHHHHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEE
VEGDEMIINGQRIRTTQERDIDAIDWSGCDVVLEATGVHRKASFLNKYLEQGVKRVVVSA
EECCEEEECCCEEECCHHCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEHCC
PVKEEGIANIVVGVNDNIFDPAVHKIVTAASCTTNCLAPVVKVINEKLGIENAAFTTIHD
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELENRINGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCH
LTDIIFEVKQDTTAEEVNAMLKEASENELKGILGFEERPLVSIDYKGDQRSTIVDALSTM
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
LVGKRMVKIYAWYDNEMGYATRTAELVRTVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVKVGINGFGRIGRLALRAAFDWAEIEFVQINDVAGDTATLAHLLEFDSVQGRWNHEVA
EEEEECCCCCHHHHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEE
VEGDEMIINGQRIRTTQERDIDAIDWSGCDVVLEATGVHRKASFLNKYLEQGVKRVVVSA
EECCEEEECCCEEECCHHCCCCEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEHCC
PVKEEGIANIVVGVNDNIFDPAVHKIVTAASCTTNCLAPVVKVINEKLGIENAAFTTIHD
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIVEIFPELENRINGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCH
LTDIIFEVKQDTTAEEVNAMLKEASENELKGILGFEERPLVSIDYKGDQRSTIVDALSTM
HHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
LVGKRMVKIYAWYDNEMGYATRTAELVRTVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]