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The map label for this gene is purH [H]

Identifier: 218710867

GI number: 218710867

Start: 3109472

End: 3111064

Strand: Direct

Name: purH [H]

Synonym: VS_2951

Alternate gene names: 218710867

Gene position: 3109472-3111064 (Clockwise)

Preceding gene: 218710865

Following gene: 218710868

Centisome position: 94.25

GC content: 49.09

Gene sequence:

>1593_bases
ATGAATAACGCTCGTCCAATTCGCCGCGCTCTAATCAGCGTATCAGACAAAACTGGTATCGTTGAATTTGCACAAGCTCT
TGCTAACCGTGGTGTAGATATCCTATCTACCGGTGGCACTGCTCGCCTGCTTGCTGAAAAAGGCATCTCTGTTACAGAAG
TATCTGATTACACTGGTTTCCCAGAAATGATGGATGGCCGTGTTAAGACTCTGCACCCAAAAGTTCATGGTGGTGTTCTA
GGCCGTCGTGGCCAAGATGATGACGTGATGGAAACTCACGGTATCAACCCTATCGATATGGTTGTTGTAAACCTATACCC
ATTCGCAGAAACCGTTGCTAAAGAAGGTTGTACCCTTGCTGACGCTGTTGAGAACATCGACATCGGCGGTCCTACAATGG
TTCGCTCTGCAGCGAAAAACCACAAAGACGTAACTATCGTTGTTAACGCACACGACTACGAGCGCGTTGTTGCTGAAATG
GACGCGAACGAGAAATCTCTAACGCTAGAGACTCGCTTCGACCTCGCTATCGCAGCATTCGAGCACACAGCTTCTTACGA
CGGCATGATCGCAAACTATTTCGGCACTATGGTTCCTTCTTATGGAGAGAACAAAGAAGGTGACGAAGAGAGCAAATTCC
CTCGCACGTTCAACCAACAGTTCGAGAAAAAGCAAGACATGCGCTACGGTGAGAACAGCCACCAAGCAGCGGCATTCTAC
GTTGAAGCAAACCCTGAAGAAGCGTCTGTTTCTACTGCTCGCCAAATCCAAGGTAAAGCGCTTTCTTACAACAACATTGC
TGACACAGATGCAGCACTTGAGTGTGTGAAAGAGTTCGACGAGCCTGCATGTGTGATCGTTAAGCACGCTAACCCATGTG
GCGTTGCACTAGGTGAAGACATCCTAGAAGCTTACGACCGTGCATTCAAAACAGACCCAACGTCTGCATTTGGCGGCATC
ATCGCTTTCAACCGTGAGCTAGACGCTGCAACAGCAACGGCTATCACTGAGCGTCAATTCGTTGAAGTTATCATTGCACC
ATCTGTTTCTGCGGAAGCAGTAGCAATCGTAGCGGCTAAGAAAAACCTTCGCCTGCTTGAGTGCGGTGAGTGGACAACTA
AGACAACTGGTTTTGACGTGAAACGCGTTAACGGCGGTTTGCTAGTTCAAGACCGCGACCAAGGTATGGTTTCTGAAGAT
GACCTGAAAGTGGTTTCTAAGCGTCAGCCAACAGCTGAAGAATTGAAAGATGCTCTATTCTGCTGGAAAGTAGCGAAGTA
CGTTAAATCTAACGCTATCGTTTACTCGAAAGGCGACATGACTATTGGTGTAGGCGCAGGCCAAATGAGCCGCGTTTACT
CTGCGAAAATCGCAGGTATCAAAGCGGCAGACGAAGGTCTACAGGTTGAAGGTTGCGTGATGGCATCAGATGCATTCTTC
CCATTCCGTGACGGTATCGACGCGGCAGCAGAAGCGGGCATCAAGTGTGTTATCCAACCGGGCGGCTCTATGCGTGATGA
CGAAGTTATCGCAGCAGCAGACGAACACGGCATGGCGATGATCTTTACGGGCATGCGTCACTTCCGCCACTAA

Upstream 100 bases:

>100_bases
TTACTGGTATCTAAGATAATCCACGCTAAGAAGATAGAAACAAGTTCTTTTTTGGCAAATATCTTTATTTTAACCCCATG
AATTTGAGGAAGATGGAAGC

Downstream 100 bases:

>100_bases
TTTCCTCTTCGTATTTGGCACTGTGGCGTCGTTAACTGCTTTCGCCCACCCCGGTCACATAGCTGGCTATGTGACCGGGG
ATGGGCTCAATTGCTGCCTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNNARPIRRALISVSDKTGIVEFAQALANRGVDILSTGGTARLLAEKGISVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDDVMETHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYERVVAEM
DANEKSLTLETRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGEDILEAYDRAFKTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSAEAVAIVAAKKNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSED
DLKVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH

Sequences:

>Translated_530_residues
MNNARPIRRALISVSDKTGIVEFAQALANRGVDILSTGGTARLLAEKGISVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDDVMETHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYERVVAEM
DANEKSLTLETRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGEDILEAYDRAFKTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSAEAVAIVAAKKNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSED
DLKVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
>Mature_530_residues
MNNARPIRRALISVSDKTGIVEFAQALANRGVDILSTGGTARLLAEKGISVTEVSDYTGFPEMMDGRVKTLHPKVHGGVL
GRRGQDDDVMETHGINPIDMVVVNLYPFAETVAKEGCTLADAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYERVVAEM
DANEKSLTLETRFDLAIAAFEHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGEDILEAYDRAFKTDPTSAFGGI
IAFNRELDAATATAITERQFVEVIIAPSVSAEAVAIVAAKKNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSED
DLKVVSKRQPTAEELKDALFCWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=517, Percent_Identity=36.9439071566731, Blast_Score=275, Evalue=5e-74,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=78.5310734463277, Blast_Score=871, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=513, Percent_Identity=32.5536062378168, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=36.9230769230769, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985574, Length=373, Percent_Identity=24.6648793565684, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=618, Percent_Identity=33.3333333333333, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6323768, Length=491, Percent_Identity=35.4378818737271, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24649832, Length=614, Percent_Identity=35.1791530944625, Blast_Score=308, Evalue=5e-84,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57425; Mature: 57425

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNARPIRRALISVSDKTGIVEFAQALANRGVDILSTGGTARLLAEKGISVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEHHHHCCCCEEEECCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDDVMETHGINPIDMVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCCHHHHCCCCHHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYERVVAEMDANEKSLTLETRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCEEEEEH
EHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGED
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
ILEAYDRAFKTDPTSAFGGIIAFNRELDAATATAITERQFVEVIIAPSVSAEAVAIVAAK
HHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHEEEEEEEECCCCCCEEEEEEEC
KNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSEDDLKVVSKRQPTAEELKDALF
CCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MNNARPIRRALISVSDKTGIVEFAQALANRGVDILSTGGTARLLAEKGISVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEHHHHCCCCEEEECCCCCC
PEMMDGRVKTLHPKVHGGVLGRRGQDDDVMETHGINPIDMVVVNLYPFAETVAKEGCTLA
HHHHCCCEEEECCHHCCCCCCCCCCCCCHHHHCCCCHHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVTIVVNAHDYERVVAEMDANEKSLTLETRFDLAIAAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCEEEEEH
EHTASYDGMIANYFGTMVPSYGENKEGDEESKFPRTFNQQFEKKQDMRYGENSHQAAAFY
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
VEANPEEASVSTARQIQGKALSYNNIADTDAALECVKEFDEPACVIVKHANPCGVALGED
EECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
ILEAYDRAFKTDPTSAFGGIIAFNRELDAATATAITERQFVEVIIAPSVSAEAVAIVAAK
HHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHEEEEEEEECCCCCCEEEEEEEC
KNLRLLECGEWTTKTTGFDVKRVNGGLLVQDRDQGMVSEDDLKVVSKRQPTAEELKDALF
CCCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHH
CWKVAKYVKSNAIVYSKGDMTIGVGAGQMSRVYSAKIAGIKAADEGLQVEGCVMASDAFF
HHHHHHHHHCCEEEEECCCEEEEECCCHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCC
PFRDGIDAAAEAGIKCVIQPGGSMRDDEVIAAADEHGMAMIFTGMRHFRH
CCCCCCCHHHCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA