Click here to switch to the map view.

The map label for this gene is pyrI [H]

Identifier: 218710845

GI number: 218710845

Start: 3082315

End: 3082779

Strand: Direct

Name: pyrI [H]

Synonym: VS_2928

Alternate gene names: 218710845

Gene position: 3082315-3082779 (Clockwise)

Preceding gene: 218710844

Following gene: 218710846

Centisome position: 93.42

GC content: 42.58

Gene sequence:

>465_bases
ATGGTTAAACAAACTCAGCTACAAGTAGAAGCAATCCGTAACGGCAGCGTGATTGACCACATCCCTGCTCATCTTGGTAT
CAAAATCCTTAAACTGTTTAAGCTACACAAAACAGAACAACGCATCACCATGGGATTGAATCTGCCATCATCTGCGCTTG
GTCACAAAGATCTGATCAAGATTGAGAACATCTTTCTGACCAAGGAACAAGCCAACCAATTGGCTCTGTATGCGCCGCAA
GCGACGGTAAATCAGATTGAAGAGTACAAGGTGGTTAATAAGCTAACATTAGTACTGCCAGACCAAATCAACAGTGTGTA
CTCTTGCCCGAATAGCAACTGTATTTCGCACGGTGAACCCGTTGAAAGTAGCTTTAAAGTGCAGCTAAAACACGAAAACG
TCCAACTAAAATGTCACTATTGTGAAAAAGTATTCTCTCGCGAGATCATGAGTGAAATCCGCTAG

Upstream 100 bases:

>100_bases
ACACGCTTACTACTTTGAACAAGCTGAAAACGGTGTATACGCTCGCGAAGCCCTATTGGCCCTAGTTCTTAACGACACTT
TATAAGCAGGAGAAACAGTC

Downstream 100 bases:

>100_bases
CCCTTAGCGCTCTTACCTTCGTAAAAGAAACCGCTCAAACTACATGGATATTTATTCCCAACCCGGAATAAATATCCACT
TACCCAAAGAATAAAATTCC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MVKQTQLQVEAIRNGSVIDHIPAHLGIKILKLFKLHKTEQRITMGLNLPSSALGHKDLIKIENIFLTKEQANQLALYAPQ
ATVNQIEEYKVVNKLTLVLPDQINSVYSCPNSNCISHGEPVESSFKVQLKHENVQLKCHYCEKVFSREIMSEIR

Sequences:

>Translated_154_residues
MVKQTQLQVEAIRNGSVIDHIPAHLGIKILKLFKLHKTEQRITMGLNLPSSALGHKDLIKIENIFLTKEQANQLALYAPQ
ATVNQIEEYKVVNKLTLVLPDQINSVYSCPNSNCISHGEPVESSFKVQLKHENVQLKCHYCEKVFSREIMSEIR
>Mature_154_residues
MVKQTQLQVEAIRNGSVIDHIPAHLGIKILKLFKLHKTEQRITMGLNLPSSALGHKDLIKIENIFLTKEQANQLALYAPQ
ATVNQIEEYKVVNKLTLVLPDQINSVYSCPNSNCISHGEPVESSFKVQLKHENVQLKCHYCEKVFSREIMSEIR

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=150, Percent_Identity=60.6666666666667, Blast_Score=197, Evalue=3e-52,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 17518; Mature: 17518

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKQTQLQVEAIRNGSVIDHIPAHLGIKILKLFKLHKTEQRITMGLNLPSSALGHKDLIK
CCCCHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHCCCHHHEE
IENIFLTKEQANQLALYAPQATVNQIEEYKVVNKLTLVLPDQINSVYSCPNSNCISHGEP
HHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHEEEECHHHHHHHCCCCCCCCCCCCC
VESSFKVQLKHENVQLKCHYCEKVFSREIMSEIR
CCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKQTQLQVEAIRNGSVIDHIPAHLGIKILKLFKLHKTEQRITMGLNLPSSALGHKDLIK
CCCCHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHCCCHHHEE
IENIFLTKEQANQLALYAPQATVNQIEEYKVVNKLTLVLPDQINSVYSCPNSNCISHGEP
HHHEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHEEEECHHHHHHHCCCCCCCCCCCCC
VESSFKVQLKHENVQLKCHYCEKVFSREIMSEIR
CCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA