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The map label for this gene is pyrB [H]

Identifier: 218710844

GI number: 218710844

Start: 3081370

End: 3082299

Strand: Direct

Name: pyrB [H]

Synonym: VS_2927

Alternate gene names: 218710844

Gene position: 3081370-3082299 (Clockwise)

Preceding gene: 218710843

Following gene: 218710845

Centisome position: 93.39

GC content: 48.17

Gene sequence:

>930_bases
ATGGCGCATTCGTTATTTAACAAGCACATCATCTCCATTCCAGAGCTATCTCGTGATGAACTGGAGCTTATCGTCGATAC
TGCAGCAAGACTCAAAGCAGAACCAAACCCTGAGCTGCTGAAAAATAAAGTCGTTGCGAGCTGCTTCTTTGAGCCTTCAA
CTCGAACTCGCCTCTCATTTGAAACCGCAGTTCAACGCCTTGGCGGCACAGTCATCGGCTTTGATAATGGCGGCAACACA
TCGCTGGCGAAAAAAGGCGAAACACTTGCCGATTCCGTTCAAGTGATTTCATCTTACGTGGATGCCTTTGTGATGCGTCA
CCCACAAGAAGGCGCGGCGCGCTTAGCGTCTGAATTTTCTAACGGTGTGCCAATTGTGAATGGTGGCGACGGTGCAAACC
AACACCCAACGCAAACCTTATTGGATCTGTTCTCGATTTACGAAACGCAAGGCACGCTCGATAACCTCAATGTGGCTTTC
GTCGGCGACCTAAAATATGGCCGTACCGTACACTCTTTGACGCAAGCACTATCAAAATTCAATAACGTGCGTTTCTTCTT
TATCGCGCCAGAAGTGTTAGCGATGCCTGATTACATCTGTGAAGAGTTGGAAGAGATGGGCATCCAATACAGCACCCACA
GCAGCATTGAAGAAGTGGTGCCTGAACTGGATGTCCTGTACATGACTCGCGTACAGAAAGAGCGCTTTGATGCGTCAGAA
TACGCGCACATGAAGTCGGCTTACATTCTAACGGCCGATACCTTGAAAGATGCACGCCAGAACATGAAGGTGCTTCACCC
ATTACCACGCGTCGATGAAATCACAACCGACGTAGATAAAACGCCACACGCTTACTACTTTGAACAAGCTGAAAACGGTG
TATACGCTCGCGAAGCCCTATTGGCCCTAGTTCTTAACGACACTTTATAA

Upstream 100 bases:

>100_bases
TTTTTATTTTGCTCTATTGATCATTTCGAAGCCTCCTATTTTTAGGGGGCTTTTTTTTGTCCGCAATTTGACTGTATGCA
TAGAAAAAGGAAGACCCGTT

Downstream 100 bases:

>100_bases
GCAGGAGAAACAGTCATGGTTAAACAAACTCAGCTACAAGTAGAAGCAATCCGTAACGGCAGCGTGATTGACCACATCCC
TGCTCATCTTGGTATCAAAA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MAHSLFNKHIISIPELSRDELELIVDTAARLKAEPNPELLKNKVVASCFFEPSTRTRLSFETAVQRLGGTVIGFDNGGNT
SLAKKGETLADSVQVISSYVDAFVMRHPQEGAARLASEFSNGVPIVNGGDGANQHPTQTLLDLFSIYETQGTLDNLNVAF
VGDLKYGRTVHSLTQALSKFNNVRFFFIAPEVLAMPDYICEELEEMGIQYSTHSSIEEVVPELDVLYMTRVQKERFDASE
YAHMKSAYILTADTLKDARQNMKVLHPLPRVDEITTDVDKTPHAYYFEQAENGVYAREALLALVLNDTL

Sequences:

>Translated_309_residues
MAHSLFNKHIISIPELSRDELELIVDTAARLKAEPNPELLKNKVVASCFFEPSTRTRLSFETAVQRLGGTVIGFDNGGNT
SLAKKGETLADSVQVISSYVDAFVMRHPQEGAARLASEFSNGVPIVNGGDGANQHPTQTLLDLFSIYETQGTLDNLNVAF
VGDLKYGRTVHSLTQALSKFNNVRFFFIAPEVLAMPDYICEELEEMGIQYSTHSSIEEVVPELDVLYMTRVQKERFDASE
YAHMKSAYILTADTLKDARQNMKVLHPLPRVDEITTDVDKTPHAYYFEQAENGVYAREALLALVLNDTL
>Mature_308_residues
AHSLFNKHIISIPELSRDELELIVDTAARLKAEPNPELLKNKVVASCFFEPSTRTRLSFETAVQRLGGTVIGFDNGGNTS
LAKKGETLADSVQVISSYVDAFVMRHPQEGAARLASEFSNGVPIVNGGDGANQHPTQTLLDLFSIYETQGTLDNLNVAFV
GDLKYGRTVHSLTQALSKFNNVRFFFIAPEVLAMPDYICEELEEMGIQYSTHSSIEEVVPELDVLYMTRVQKERFDASEY
AHMKSAYILTADTLKDARQNMKVLHPLPRVDEITTDVDKTPHAYYFEQAENGVYAREALLALVLNDTL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=45.6591639871383, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=71.1974110032362, Blast_Score=473, Evalue=1e-135,
Organism=Escherichia coli, GI1786469, Length=341, Percent_Identity=23.7536656891496, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=39.4495412844037, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=42.3948220064725, Blast_Score=243, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322373, Length=327, Percent_Identity=25.3822629969419, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=46.1538461538462, Blast_Score=270, Evalue=7e-73,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34409; Mature: 34278

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSLFNKHIISIPELSRDELELIVDTAARLKAEPNPELLKNKVVASCFFEPSTRTRLSF
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
ETAVQRLGGTVIGFDNGGNTSLAKKGETLADSVQVISSYVDAFVMRHPQEGAARLASEFS
HHHHHHHCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGVPIVNGGDGANQHPTQTLLDLFSIYETQGTLDNLNVAFVGDLKYGRTVHSLTQALSKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
NNVRFFFIAPEVLAMPDYICEELEEMGIQYSTHSSIEEVVPELDVLYMTRVQKERFDASE
CCEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
YAHMKSAYILTADTLKDARQNMKVLHPLPRVDEITTDVDKTPHAYYFEQAENGVYAREAL
HHHHHHEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEHHHHCCCHHHHHHH
LALVLNDTL
HHHHHHCCC
>Mature Secondary Structure 
AHSLFNKHIISIPELSRDELELIVDTAARLKAEPNPELLKNKVVASCFFEPSTRTRLSF
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
ETAVQRLGGTVIGFDNGGNTSLAKKGETLADSVQVISSYVDAFVMRHPQEGAARLASEFS
HHHHHHHCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGVPIVNGGDGANQHPTQTLLDLFSIYETQGTLDNLNVAFVGDLKYGRTVHSLTQALSKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
NNVRFFFIAPEVLAMPDYICEELEEMGIQYSTHSSIEEVVPELDVLYMTRVQKERFDASE
CCEEEEEECCHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
YAHMKSAYILTADTLKDARQNMKVLHPLPRVDEITTDVDKTPHAYYFEQAENGVYAREAL
HHHHHHEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEHHHHCCCHHHHHHH
LALVLNDTL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA