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The map label for this gene is aspA [H]

Identifier: 218710838

GI number: 218710838

Start: 3071768

End: 3073219

Strand: Direct

Name: aspA [H]

Synonym: VS_2921

Alternate gene names: 218710838

Gene position: 3071768-3073219 (Clockwise)

Preceding gene: 218710836

Following gene: 218710839

Centisome position: 93.1

GC content: 44.83

Gene sequence:

>1452_bases
ATGGCTAATCTATCAGAAGCTCAAGTTGAAGCTCCTCAAGCTACTCGTCTCGAAGAAGATCTACTAGGTCAACGTCATGT
TCCGGCTGATGCTTACTACGGCATCCACACTCTACGCGCAGTTGAAAACTTCAACATCTCAAATGTAACGATCTCAGATG
TACCTGAATTCGTTCGCGGTATGGTGATGACAAAGAAAGCAGCGGCTTTAGCAAACAAAGAGTTAGGTGTAATCCCAAGC
GAAGTAGCTAAATACATCATCGAAGCTTGTGACCTCATTCTAGACACTGGTAAGTGCATGGATCAGTTCCCATCGGATGT
ATTCCAAGGTGGTGCTGGTACTTCTGTAAACATGAATGCTAACGAAGTTGTGGCAAACGTGGCACTTGAACTTATGGGCA
AAGAAAAAGGCCAATATGAGTTCATCAACCCGAATGACCATGTAAACCGCAGCCAATCAACAAACTGTGCTTACCCAACT
GGTTTCCGTATCTCAGTTTACAACAGCGTTCGTAAACTGATTGACGCTATCGAATACCTAAAAGGCGCATTCGAGCTTAA
GAGCCAAGAATTCAACACAATCCTGAAGATGGGCCGTACTCAACTTCAAGATGCTGTTCCAATGACGGTTGGCCAAGAGT
TCCACGCTTGGGCTGTAACCATCAACGAAGAAATCAAAAACCTAGAATACACTTCAAAGCTTCTTCTTGAAGTTAACCTA
GGCGCAACGGCTATCGGTACTGGTCTGAACGCAGCACCGGGTTACCAAGGCCTAGCAGTGAAACACCTAGCAGAAGTAAC
GGGCTTAGAGTGTGTAGCGGCTGAAGATCTTATCGAAGCAACATCTGACTGTGGCGCATACGTAATGACGCACGGTGCAC
TTAAGCGCCTAGCGGTTAAACTGTCTAAGATTTGTAACGACTTACGTCTTCTTTCTTCAGGCCCACGCACTGGCTTTAAC
GAACTAAACCTACCTGAACTGCAAGCGGGCTCTTCAATCATGCCAGCTAAAGTAAACCCAGTAGTGCCTGAAGTAGTAAA
CCAAGTTTGCTTTAAAGTTCTAGGTAACGACAACACGGTTTCTTTCGCAGCAGAAGGCGGTCAGCTTCAACTGAACGTAA
TGGAACCTGTTATCGCACAAAGCATGTTTGAGTCTCTAGACATTCTAACAAACGCATGTGTGAACCTACGCGACAAGTGT
GTAGACGGCATTACTGTGAACAAAGAAGTGTGTGAATCTCACGTATTTAACTCTATCGGTATCGTCACTTACCTAAACCC
ATACATTGGCCACCACGAGGGTGATGTTGTTGGTAAGATCTGTGCTGAAACAGGTAAAAGCGTGCGTGATGTGGTTCTAG
AGCGCGGGTTATTAACCGAAGAAGAACTTGATGACATTTTCTCTGTTGAAAATTTGATGCATCCTCAGTATAAAGCAAAA
CGCTACGAATAA

Upstream 100 bases:

>100_bases
ACGGATTTATCAGCCCAATATTCTAGCGAACGGAGTATTTGCACACAGAATAGGCTAATAACTAACTATATAATCAGAAT
AATTCTCTAAGGATCCTGTT

Downstream 100 bases:

>100_bases
CGTTAGCGAAAAAGGGCTCCAAACGGAGCCCTTTTTTAGGCTCCAATACGGTTAACCATGTTAATTTTGTGGGTTTCTGT
TGGTTGCACTTTTGCGAAAT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MANLSEAQVEAPQATRLEEDLLGQRHVPADAYYGIHTLRAVENFNISNVTISDVPEFVRGMVMTKKAAALANKELGVIPS
EVAKYIIEACDLILDTGKCMDQFPSDVFQGGAGTSVNMNANEVVANVALELMGKEKGQYEFINPNDHVNRSQSTNCAYPT
GFRISVYNSVRKLIDAIEYLKGAFELKSQEFNTILKMGRTQLQDAVPMTVGQEFHAWAVTINEEIKNLEYTSKLLLEVNL
GATAIGTGLNAAPGYQGLAVKHLAEVTGLECVAAEDLIEATSDCGAYVMTHGALKRLAVKLSKICNDLRLLSSGPRTGFN
ELNLPELQAGSSIMPAKVNPVVPEVVNQVCFKVLGNDNTVSFAAEGGQLQLNVMEPVIAQSMFESLDILTNACVNLRDKC
VDGITVNKEVCESHVFNSIGIVTYLNPYIGHHEGDVVGKICAETGKSVRDVVLERGLLTEEELDDIFSVENLMHPQYKAK
RYE

Sequences:

>Translated_483_residues
MANLSEAQVEAPQATRLEEDLLGQRHVPADAYYGIHTLRAVENFNISNVTISDVPEFVRGMVMTKKAAALANKELGVIPS
EVAKYIIEACDLILDTGKCMDQFPSDVFQGGAGTSVNMNANEVVANVALELMGKEKGQYEFINPNDHVNRSQSTNCAYPT
GFRISVYNSVRKLIDAIEYLKGAFELKSQEFNTILKMGRTQLQDAVPMTVGQEFHAWAVTINEEIKNLEYTSKLLLEVNL
GATAIGTGLNAAPGYQGLAVKHLAEVTGLECVAAEDLIEATSDCGAYVMTHGALKRLAVKLSKICNDLRLLSSGPRTGFN
ELNLPELQAGSSIMPAKVNPVVPEVVNQVCFKVLGNDNTVSFAAEGGQLQLNVMEPVIAQSMFESLDILTNACVNLRDKC
VDGITVNKEVCESHVFNSIGIVTYLNPYIGHHEGDVVGKICAETGKSVRDVVLERGLLTEEELDDIFSVENLMHPQYKAK
RYE
>Mature_482_residues
ANLSEAQVEAPQATRLEEDLLGQRHVPADAYYGIHTLRAVENFNISNVTISDVPEFVRGMVMTKKAAALANKELGVIPSE
VAKYIIEACDLILDTGKCMDQFPSDVFQGGAGTSVNMNANEVVANVALELMGKEKGQYEFINPNDHVNRSQSTNCAYPTG
FRISVYNSVRKLIDAIEYLKGAFELKSQEFNTILKMGRTQLQDAVPMTVGQEFHAWAVTINEEIKNLEYTSKLLLEVNLG
ATAIGTGLNAAPGYQGLAVKHLAEVTGLECVAAEDLIEATSDCGAYVMTHGALKRLAVKLSKICNDLRLLSSGPRTGFNE
LNLPELQAGSSIMPAKVNPVVPEVVNQVCFKVLGNDNTVSFAAEGGQLQLNVMEPVIAQSMFESLDILTNACVNLRDKCV
DGITVNKEVCESHVFNSIGIVTYLNPYIGHHEGDVVGKICAETGKSVRDVVLERGLLTEEELDDIFSVENLMHPQYKAKR
YE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=471, Percent_Identity=37.1549893842887, Blast_Score=321, Evalue=9e-88,
Organism=Escherichia coli, GI87082375, Length=467, Percent_Identity=77.3019271948608, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=38.1263616557734, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1790398, Length=319, Percent_Identity=25.3918495297806, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=40.04329004329, Blast_Score=340, Evalue=7e-94,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=39.4495412844037, Blast_Score=244, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=40.04329004329, Blast_Score=343, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=39.0086206896552, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24640177, Length=467, Percent_Identity=38.9721627408994, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24640179, Length=467, Percent_Identity=38.9721627408994, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=37.5, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24583245, Length=457, Percent_Identity=34.1356673960613, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52666; Mature: 52535

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLSEAQVEAPQATRLEEDLLGQRHVPADAYYGIHTLRAVENFNISNVTISDVPEFVRG
CCCCCCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MVMTKKAAALANKELGVIPSEVAKYIIEACDLILDTGKCMDQFPSDVFQGGAGTSVNMNA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCEECCCH
NEVVANVALELMGKEKGQYEFINPNDHVNRSQSTNCAYPTGFRISVYNSVRKLIDAIEYL
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KGAFELKSQEFNTILKMGRTQLQDAVPMTVGQEFHAWAVTINEEIKNLEYTSKLLLEVNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEEHHHHHHHHHHHHEEEEECC
GATAIGTGLNAAPGYQGLAVKHLAEVTGLECVAAEDLIEATSDCGAYVMTHGALKRLAVK
CCHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSKICNDLRLLSSGPRTGFNELNLPELQAGSSIMPAKVNPVVPEVVNQVCFKVLGNDNTV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
SFAAEGGQLQLNVMEPVIAQSMFESLDILTNACVNLRDKCVDGITVNKEVCESHVFNSIG
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
IVTYLNPYIGHHEGDVVGKICAETGKSVRDVVLERGLLTEEELDDIFSVENLMHPQYKAK
HHHHHCHHHCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHC
RYE
CCC
>Mature Secondary Structure 
ANLSEAQVEAPQATRLEEDLLGQRHVPADAYYGIHTLRAVENFNISNVTISDVPEFVRG
CCCCCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MVMTKKAAALANKELGVIPSEVAKYIIEACDLILDTGKCMDQFPSDVFQGGAGTSVNMNA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCEECCCH
NEVVANVALELMGKEKGQYEFINPNDHVNRSQSTNCAYPTGFRISVYNSVRKLIDAIEYL
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KGAFELKSQEFNTILKMGRTQLQDAVPMTVGQEFHAWAVTINEEIKNLEYTSKLLLEVNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEEHHHHHHHHHHHHEEEEECC
GATAIGTGLNAAPGYQGLAVKHLAEVTGLECVAAEDLIEATSDCGAYVMTHGALKRLAVK
CCHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSKICNDLRLLSSGPRTGFNELNLPELQAGSSIMPAKVNPVVPEVVNQVCFKVLGNDNTV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
SFAAEGGQLQLNVMEPVIAQSMFESLDILTNACVNLRDKCVDGITVNKEVCESHVFNSIG
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
IVTYLNPYIGHHEGDVVGKICAETGKSVRDVVLERGLLTEEELDDIFSVENLMHPQYKAK
HHHHHCHHHCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHC
RYE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]