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The map label for this gene is dcuA [H]

Identifier: 218710839

GI number: 218710839

Start: 3073401

End: 3074708

Strand: Direct

Name: dcuA [H]

Synonym: VS_2922

Alternate gene names: 218710839

Gene position: 3073401-3074708 (Clockwise)

Preceding gene: 218710838

Following gene: 218710840

Centisome position: 93.15

GC content: 48.47

Gene sequence:

>1308_bases
ATGATTGCAGTAGAACTATTTGTCGTCCTGCTCTTTATCTTTTTGGGGGCCAGAATTGGCGGTATCGGTATTGGTTTTGC
CGGTGGTGCTGGTGTTATTGCCCTTTCACTGATTCTTGGCGTTCCAACAAGCCAGTCTTTCATCCCGATCGACGTAATCT
TGATCATCATGTCGGTTATCACCGCAATTGCTGCTATGCAGGTTGCGGGCGGTATGGACTGGTTGGTACAAATTGCAGAA
AACTTTTTGCGTAAACACCCTGAACGCATCACCTTTTACGCGCCGATTGTGACCTTTGTAATGACACTAATGGCCGGTAC
TGGTCACACAGCATTCTCTACGCTTCCTGTAATCGCAGAAGTAGCGAAAGGCCAAGGTGTTCGTCCTTCTCGTCCACTGT
CTATCGCAGTTGTAGCCTCTCAAATTGCTATCACAGCTTCGCCAATCTCGGCTGCCGTTGTCGCTTTCGCAGCAATGCTG
GCACCCTTTGGTGTTGATTACCTAACGCTATTGATGGTTTGTATCCCAACAACCTTCATCGCTTGTATGGTTGGTGCGGT
TGTTGCTAACTACATGGGTAGCGAACTGAAAGACGATCCTGTTTACCAAGAACGTCTAGAGAAAGGCCTAATCAAACTGG
CGACAGAAGAGAAACGTGAAATTCTACCAACAGCGAAGAGAGCAACGTACATCTTCCTAGCAGCAATTGGTTTCGTGGTT
TGCTACGCAGCGGCTATCTCTAGCTCTGTTGGCCTAATTGAAAACCCAGCGCTTGGTCGTAACGAAGCGATCATGTCTGT
GATGCTAGCAGCAGCAGCGGCTATCGTGATGTTCACTAAGATTGATGCTGCGAAGATTTCTTCAGCGCCAACATTCCGCT
CTGGTATGACAGCGTGTGTTTGTGTACTTGGTGTAGCTTGGTTAGGTTCAACGTTTGTAAACGCACACGTTGCTGAGATC
AAAGACGTAGCAGGTGCTCTACTGGCTGACTACCCATGGATGCTGGCTCTTGTTTTGTTCTTCGCTTCTATGCTGCTTTA
CTCTCAAGGCGCAACAACAGTTGCACTGATGCCAGCAGCTCTAGCGATTGGCGTAGCACCACTAACAGCCGTGGCTTCTT
TTGCTGCAGTAAGTGCACTGTTCGTACTTCCAACTTACCCTACGCTGCTGGCAGCGGTTGAGATGGATGACACAGGCTCA
ACGCGTATTGGTAAGTACGTATTTAATCACCCATTCTTCATTCCTGGTGTGGTAACAATCAGTACAGCGGTTGCACTCGG
CTTCGCATTTGGCGGCCTATTCATCTAG

Upstream 100 bases:

>100_bases
GGTTGCACTTTTGCGAAATAGAATCTAAACCATAGAACTATTATGTGAACATTCCAGCGGCGAATGTTCACATAATAAAA
TCTAAAATGAGGTGTTACTT

Downstream 100 bases:

>100_bases
TAATCACTAATGTTCTAAACTAGAAAAGGAAGCTTCGGCTTCCTTTTTTGTTTTCTGCTCAATGTGTTATTTGATACACG
TCAAATAACCGTTATAAAAT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIAVELFVVLLFIFLGARIGGIGIGFAGGAGVIALSLILGVPTSQSFIPIDVILIIMSVITAIAAMQVAGGMDWLVQIAE
NFLRKHPERITFYAPIVTFVMTLMAGTGHTAFSTLPVIAEVAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAML
APFGVDYLTLLMVCIPTTFIACMVGAVVANYMGSELKDDPVYQERLEKGLIKLATEEKREILPTAKRATYIFLAAIGFVV
CYAAAISSSVGLIENPALGRNEAIMSVMLAAAAAIVMFTKIDAAKISSAPTFRSGMTACVCVLGVAWLGSTFVNAHVAEI
KDVAGALLADYPWMLALVLFFASMLLYSQGATTVALMPAALAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGS
TRIGKYVFNHPFFIPGVVTISTAVALGFAFGGLFI

Sequences:

>Translated_435_residues
MIAVELFVVLLFIFLGARIGGIGIGFAGGAGVIALSLILGVPTSQSFIPIDVILIIMSVITAIAAMQVAGGMDWLVQIAE
NFLRKHPERITFYAPIVTFVMTLMAGTGHTAFSTLPVIAEVAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAML
APFGVDYLTLLMVCIPTTFIACMVGAVVANYMGSELKDDPVYQERLEKGLIKLATEEKREILPTAKRATYIFLAAIGFVV
CYAAAISSSVGLIENPALGRNEAIMSVMLAAAAAIVMFTKIDAAKISSAPTFRSGMTACVCVLGVAWLGSTFVNAHVAEI
KDVAGALLADYPWMLALVLFFASMLLYSQGATTVALMPAALAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGS
TRIGKYVFNHPFFIPGVVTISTAVALGFAFGGLFI
>Mature_435_residues
MIAVELFVVLLFIFLGARIGGIGIGFAGGAGVIALSLILGVPTSQSFIPIDVILIIMSVITAIAAMQVAGGMDWLVQIAE
NFLRKHPERITFYAPIVTFVMTLMAGTGHTAFSTLPVIAEVAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAML
APFGVDYLTLLMVCIPTTFIACMVGAVVANYMGSELKDDPVYQERLEKGLIKLATEEKREILPTAKRATYIFLAAIGFVV
CYAAAISSSVGLIENPALGRNEAIMSVMLAAAAAIVMFTKIDAAKISSAPTFRSGMTACVCVLGVAWLGSTFVNAHVAEI
KDVAGALLADYPWMLALVLFFASMLLYSQGATTVALMPAALAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGS
TRIGKYVFNHPFFIPGVVTISTAVALGFAFGGLFI

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=436, Percent_Identity=56.1926605504587, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1790565, Length=397, Percent_Identity=37.27959697733, Blast_Score=250, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45376; Mature: 45376

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVELFVVLLFIFLGARIGGIGIGFAGGAGVIALSLILGVPTSQSFIPIDVILIIMSVI
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TAIAAMQVAGGMDWLVQIAENFLRKHPERITFYAPIVTFVMTLMAGTGHTAFSTLPVIAE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAMLAPFGVDYLTLLMVCIPTTFI
HHCCCCCCCCCCEEHEEEHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ACMVGAVVANYMGSELKDDPVYQERLEKGLIKLATEEKREILPTAKRATYIFLAAIGFVV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
CYAAAISSSVGLIENPALGRNEAIMSVMLAAAAAIVMFTKIDAAKISSAPTFRSGMTACV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
CVLGVAWLGSTFVNAHVAEIKDVAGALLADYPWMLALVLFFASMLLYSQGATTVALMPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKYVFNHPFFIPGVVTI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCHHHHHHHCCCCCCCHHHHH
STAVALGFAFGGLFI
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAVELFVVLLFIFLGARIGGIGIGFAGGAGVIALSLILGVPTSQSFIPIDVILIIMSVI
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TAIAAMQVAGGMDWLVQIAENFLRKHPERITFYAPIVTFVMTLMAGTGHTAFSTLPVIAE
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAKGQGVRPSRPLSIAVVASQIAITASPISAAVVAFAAMLAPFGVDYLTLLMVCIPTTFI
HHCCCCCCCCCCEEHEEEHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ACMVGAVVANYMGSELKDDPVYQERLEKGLIKLATEEKREILPTAKRATYIFLAAIGFVV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
CYAAAISSSVGLIENPALGRNEAIMSVMLAAAAAIVMFTKIDAAKISSAPTFRSGMTACV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
CVLGVAWLGSTFVNAHVAEIKDVAGALLADYPWMLALVLFFASMLLYSQGATTVALMPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LAIGVAPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKYVFNHPFFIPGVVTI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCHHHHHHHCCCCCCCHHHHH
STAVALGFAFGGLFI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]