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The map label for this gene is maeB [H]

Identifier: 218710813

GI number: 218710813

Start: 3049254

End: 3050543

Strand: Reverse

Name: maeB [H]

Synonym: VS_2896

Alternate gene names: 218710813

Gene position: 3050543-3049254 (Counterclockwise)

Preceding gene: 218710814

Following gene: 218710811

Centisome position: 92.46

GC content: 47.21

Gene sequence:

>1290_bases
ATGTCTGAAGACAGCCGCCAAGATCAATCCCCTCAAGAATTATCGCCTCAAGAACAATTCCGTCAGCAAGCTCTTGATTA
TCATGAGTTCCCAATTCCAGGCAAAATTGCCGTAGAACTGACGAAGCCTGCAAACTCTGCAGAAGACCTAGCACTGGCAT
ACAGCCCTGGCGTAGCTGAGCCTGTTCGCGAGATCGCACAGAATGTCGATAACGTTTATAAGTACACAGGTAAAGGCAAC
ATGGTTGCAGTTATCTCTAACGGCACTGCAATTCTTGGCTTAGGTAACCTTGGTCCTATTGCTTCTAAACCAGTTATGGA
AGGTAAAGCGCTACTGTTTAAGCGTTTTGCTGGTTTAGATTCTATCGATATTGAAGTAAAACACCGCACAATCGATGAGT
TCGTTGATACGGTTGCGAATATCGCAGATACATTTGGTGGTATTAACCTAGAAGACATCAAAGCACCAGACTGTTTTGAA
ATCGAGCGTCGCCTGATTGAGCGTTGTGATGTACCTGTTTTCCATGATGATCAACACGGTACTGCGATTGTAACGGCAGC
GGGTATGCTTAACGCGATCGAGCTTCAAGGTAAGAAACTGGAAGAGTGTAAGATCGTTTGTTTAGGTGCTGGCGCAGCAG
CGGTCGCTTGTATGGAGCTACTGATTAAGTGTGGCGCTCAGCGTGAGAAGATCTACATGCTTGACCGTAAAGGTGTGATC
CACACTCGTCGTGATGACTTGAATGAATACAAAGAGCTGTTCGCAAATAACACCGACAAGCGCACGCTTGAAGATGTTAT
CGAAGGCGCTGACCTGTTCTTGGGTGTATCGGGTCCTAATCTGCTACCAGCTAAGGCACTTACGCTGATGGCTGATAAGC
CTGTTGTGTTTGCATGTTCAAACCCAGATCCAGAGATCAAGCCTGAGCTTGCACATGAAGTTCGTTCTGACCTAATCATG
GGTACTGGCCGTAGTGATTACCCTAACCAAGTAAACAACGTACTTTGTTTCCCATTCATTTTCCGTGGTGCACTAGACGT
GCGTGCGAGCGAAATCAATGACGAAATGAAGCTAGCGGCGGTTAAAGCGATTCGTGAACTAGCAAAAGAAGAAGTTCCAG
CTGAAGTACTGGCAGCTGCGGGCGAGACTGCACTGGAGTTCGGTAAGGGTTACATCATTCCTAAGCCAATGGACCCACGT
CTGCTTCCTCGCGTTGCAAAAGCAGTTGCTGAAGCGGCTGTTGAGTCTGGCGTTGCTCGAATTGAGATGCCTGCCAACTA
TATGGCTTAA

Upstream 100 bases:

>100_bases
TTTTAATGATACAGATGCTTCAACCACCTAGGAACTTTGAAGTACATCCATTAACATGAACAAGATCCCCCAAATAATAA
TATCCTAGGAACCTACACCT

Downstream 100 bases:

>100_bases
TGCCTTAATAGCTTAATAGCTTAATAGCTTAATAGCTGTTGCTCTATTGAGCGAAAAAGAATAATAAAAAACCGACTCAT
AGGAGTCGGTTTTTTTTATG

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSEDSRQDQSPQELSPQEQFRQQALDYHEFPIPGKIAVELTKPANSAEDLALAYSPGVAEPVREIAQNVDNVYKYTGKGN
MVAVISNGTAILGLGNLGPIASKPVMEGKALLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFE
IERRLIERCDVPVFHDDQHGTAIVTAAGMLNAIELQGKKLEECKIVCLGAGAAAVACMELLIKCGAQREKIYMLDRKGVI
HTRRDDLNEYKELFANNTDKRTLEDVIEGADLFLGVSGPNLLPAKALTLMADKPVVFACSNPDPEIKPELAHEVRSDLIM
GTGRSDYPNQVNNVLCFPFIFRGALDVRASEINDEMKLAAVKAIRELAKEEVPAEVLAAAGETALEFGKGYIIPKPMDPR
LLPRVAKAVAEAAVESGVARIEMPANYMA

Sequences:

>Translated_429_residues
MSEDSRQDQSPQELSPQEQFRQQALDYHEFPIPGKIAVELTKPANSAEDLALAYSPGVAEPVREIAQNVDNVYKYTGKGN
MVAVISNGTAILGLGNLGPIASKPVMEGKALLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFE
IERRLIERCDVPVFHDDQHGTAIVTAAGMLNAIELQGKKLEECKIVCLGAGAAAVACMELLIKCGAQREKIYMLDRKGVI
HTRRDDLNEYKELFANNTDKRTLEDVIEGADLFLGVSGPNLLPAKALTLMADKPVVFACSNPDPEIKPELAHEVRSDLIM
GTGRSDYPNQVNNVLCFPFIFRGALDVRASEINDEMKLAAVKAIRELAKEEVPAEVLAAAGETALEFGKGYIIPKPMDPR
LLPRVAKAVAEAAVESGVARIEMPANYMA
>Mature_428_residues
SEDSRQDQSPQELSPQEQFRQQALDYHEFPIPGKIAVELTKPANSAEDLALAYSPGVAEPVREIAQNVDNVYKYTGKGNM
VAVISNGTAILGLGNLGPIASKPVMEGKALLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEI
ERRLIERCDVPVFHDDQHGTAIVTAAGMLNAIELQGKKLEECKIVCLGAGAAAVACMELLIKCGAQREKIYMLDRKGVIH
TRRDDLNEYKELFANNTDKRTLEDVIEGADLFLGVSGPNLLPAKALTLMADKPVVFACSNPDPEIKPELAHEVRSDLIMG
TGRSDYPNQVNNVLCFPFIFRGALDVRASEINDEMKLAAVKAIRELAKEEVPAEVLAAAGETALEFGKGYIIPKPMDPRL
LPRVAKAVAEAAVESGVARIEMPANYMA

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=354, Percent_Identity=28.2485875706215, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI62420880, Length=354, Percent_Identity=28.2485875706215, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI239049447, Length=354, Percent_Identity=28.2485875706215, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4505143, Length=342, Percent_Identity=26.0233918128655, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4505145, Length=353, Percent_Identity=28.0453257790368, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=27.9874213836478, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788806, Length=404, Percent_Identity=59.4059405940594, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI87081919, Length=335, Percent_Identity=26.865671641791, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17537199, Length=405, Percent_Identity=26.1728395061728, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322823, Length=309, Percent_Identity=25.8899676375405, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI78707242, Length=347, Percent_Identity=27.9538904899135, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI78707238, Length=347, Percent_Identity=27.9538904899135, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI78707240, Length=347, Percent_Identity=27.9538904899135, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281362672, Length=353, Percent_Identity=26.9121813031161, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281362674, Length=353, Percent_Identity=26.9121813031161, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21356279, Length=353, Percent_Identity=26.9121813031161, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646388, Length=416, Percent_Identity=26.4423076923077, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24646386, Length=416, Percent_Identity=26.4423076923077, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363503, Length=411, Percent_Identity=23.1143552311436, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363505, Length=411, Percent_Identity=23.1143552311436, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI78707236, Length=411, Percent_Identity=23.1143552311436, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI78707232, Length=411, Percent_Identity=23.1143552311436, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 46731; Mature: 46600

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDSRQDQSPQELSPQEQFRQQALDYHEFPIPGKIAVELTKPANSAEDLALAYSPGVAE
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCHH
PVREIAQNVDNVYKYTGKGNMVAVISNGTAILGLGNLGPIASKPVMEGKALLFKRFAGLD
HHHHHHHHHHHHEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCC
SIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIERCDVPVFHDDQHG
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCC
TAIVTAAGMLNAIELQGKKLEECKIVCLGAGAAAVACMELLIKCGAQREKIYMLDRKGVI
CEEEEHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
HTRRDDLNEYKELFANNTDKRTLEDVIEGADLFLGVSGPNLLPAKALTLMADKPVVFACS
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHEEECCCEEEEEC
NPDPEIKPELAHEVRSDLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASEINDEMKLAA
CCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHCEEEHHHHHCCHHHHHHHHCCHHHHHHH
VKAIRELAKEEVPAEVLAAAGETALEFGKGYIIPKPMDPRLLPRVAKAVAEAAVESGVAR
HHHHHHHHHHCCCHHHHHHCCHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
IEMPANYMA
EECCCCCCC
>Mature Secondary Structure 
SEDSRQDQSPQELSPQEQFRQQALDYHEFPIPGKIAVELTKPANSAEDLALAYSPGVAE
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCHH
PVREIAQNVDNVYKYTGKGNMVAVISNGTAILGLGNLGPIASKPVMEGKALLFKRFAGLD
HHHHHHHHHHHHEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCC
SIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIERCDVPVFHDDQHG
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCC
TAIVTAAGMLNAIELQGKKLEECKIVCLGAGAAAVACMELLIKCGAQREKIYMLDRKGVI
CEEEEHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
HTRRDDLNEYKELFANNTDKRTLEDVIEGADLFLGVSGPNLLPAKALTLMADKPVVFACS
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHEEECCCEEEEEC
NPDPEIKPELAHEVRSDLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASEINDEMKLAA
CCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHCEEEHHHHHCCHHHHHHHHCCHHHHHHH
VKAIRELAKEEVPAEVLAAAGETALEFGKGYIIPKPMDPRLLPRVAKAVAEAAVESGVAR
HHHHHHHHHHCCCHHHHHHCCHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
IEMPANYMA
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]