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The map label for this gene is gph [H]

Identifier: 218710784

GI number: 218710784

Start: 3014888

End: 3015574

Strand: Reverse

Name: gph [H]

Synonym: VS_2867

Alternate gene names: 218710784

Gene position: 3015574-3014888 (Counterclockwise)

Preceding gene: 218710785

Following gene: 218710783

Centisome position: 91.4

GC content: 46.43

Gene sequence:

>687_bases
ATGTCATTAAGCTCAATAAAACTGATTGCCTTTGATTTGGATGGAACCTTGTTAGATAGCGTGCCTGATTTGGCTGTTGC
CGCCGATCAGGCTTGTCAGGAACTGGGTTTTTCTTCTGTAAGCGAAGAGCAAGTTCGCGACTACGTGGGTAATGGTGCTG
ACGTTCTTATCGGGCGCTCACTGAGCCGCAACTTGACGGTTGATCCAAACCTAGAGCCTGAACTGCTAAAGAAAGCGCGT
ATCTTGTTTGATGATTTTTACGAGCAGAGTGGCCATAAGCTGAGCCACCTTTACCCATCAGTAAAAGAAACGTTAGCTGA
GTTGGATAAAGCGGGCTTCACCATGGCGCTTGTAACAAACAAGCCATCGAAATTTGTACCAGCCGTTCTGGCGCAACACG
GTATCGATAAGTACTTTGTTGATGTGTTGGGCGGCGACTCTTTCCCAGAGAAAAAGCCGAATCCAGTAGCGTTGAACTGG
TTGCTAGAAAAGCACAATGTAAAAGCTGAAGAGATGCTGATGGTTGGCGATTCAAGCAATGACATCAAAGCGGCTAAGAA
TGCCGGCTGTCACTCGTTTGGTCTGACATACGGCTACAACCACGGTGAGTCGATTTCAGTATCAAACCCTGACTATGTCG
CAGACAATATTGCGCAATTACTAGATGTCGTTCTAGTTTCAGCATAA

Upstream 100 bases:

>100_bases
GTAAAACTACAGATAGATTGAATATTAAGGGGCTCATTGGCCCCTTTTTTACGTCTTGGAGCTCAACTCATAAGACGGAC
ATAATAAATAGGAAAGTAAG

Downstream 100 bases:

>100_bases
AGCGGATAAAAGTTAGCTAACCTACTAGCAAAATACTTATCAATGAGTACACTGGATGAGCCGTACAGAAGAGCTGTACG
GCTTTCTATTTTAAAGTTAA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSLSSIKLIAFDLDGTLLDSVPDLAVAADQACQELGFSSVSEEQVRDYVGNGADVLIGRSLSRNLTVDPNLEPELLKKAR
ILFDDFYEQSGHKLSHLYPSVKETLAELDKAGFTMALVTNKPSKFVPAVLAQHGIDKYFVDVLGGDSFPEKKPNPVALNW
LLEKHNVKAEEMLMVGDSSNDIKAAKNAGCHSFGLTYGYNHGESISVSNPDYVADNIAQLLDVVLVSA

Sequences:

>Translated_228_residues
MSLSSIKLIAFDLDGTLLDSVPDLAVAADQACQELGFSSVSEEQVRDYVGNGADVLIGRSLSRNLTVDPNLEPELLKKAR
ILFDDFYEQSGHKLSHLYPSVKETLAELDKAGFTMALVTNKPSKFVPAVLAQHGIDKYFVDVLGGDSFPEKKPNPVALNW
LLEKHNVKAEEMLMVGDSSNDIKAAKNAGCHSFGLTYGYNHGESISVSNPDYVADNIAQLLDVVLVSA
>Mature_227_residues
SLSSIKLIAFDLDGTLLDSVPDLAVAADQACQELGFSSVSEEQVRDYVGNGADVLIGRSLSRNLTVDPNLEPELLKKARI
LFDDFYEQSGHKLSHLYPSVKETLAELDKAGFTMALVTNKPSKFVPAVLAQHGIDKYFVDVLGGDSFPEKKPNPVALNWL
LEKHNVKAEEMLMVGDSSNDIKAAKNAGCHSFGLTYGYNHGESISVSNPDYVADNIAQLLDVVLVSA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=230, Percent_Identity=43.4782608695652, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24728; Mature: 24597

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSSIKLIAFDLDGTLLDSVPDLAVAADQACQELGFSSVSEEQVRDYVGNGADVLIGRS
CCCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECC
LSRNLTVDPNLEPELLKKARILFDDFYEQSGHKLSHLYPSVKETLAELDKAGFTMALVTN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEC
KPSKFVPAVLAQHGIDKYFVDVLGGDSFPEKKPNPVALNWLLEKHNVKAEEMLMVGDSSN
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCHHHEEEECCCCC
DIKAAKNAGCHSFGLTYGYNHGESISVSNPDYVADNIAQLLDVVLVSA
CHHHHHCCCCCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLSSIKLIAFDLDGTLLDSVPDLAVAADQACQELGFSSVSEEQVRDYVGNGADVLIGRS
CCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECC
LSRNLTVDPNLEPELLKKARILFDDFYEQSGHKLSHLYPSVKETLAELDKAGFTMALVTN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEC
KPSKFVPAVLAQHGIDKYFVDVLGGDSFPEKKPNPVALNWLLEKHNVKAEEMLMVGDSSN
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCHHHEEEECCCCC
DIKAAKNAGCHSFGLTYGYNHGESISVSNPDYVADNIAQLLDVVLVSA
CHHHHHCCCCCCCCCEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA