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The map label for this gene is tuf1 [H]

Identifier: 218710751

GI number: 218710751

Start: 2980744

End: 2981928

Strand: Reverse

Name: tuf1 [H]

Synonym: VS_2834

Alternate gene names: 218710751

Gene position: 2981928-2980744 (Counterclockwise)

Preceding gene: 218710752

Following gene: 218710750

Centisome position: 90.38

GC content: 46.5

Gene sequence:

>1185_bases
GTGTCTAAAGAAAAATTTGAACGTACGAAACCGCACGTAAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACAAC
TCTAACTGCTGCTATCTGTACTACACTTGCAAAAGTGTACGGCGGTGTTGCTAAAGATTTCGCATCTATCGATAACGCTC
CAGAAGAGCGCGAGCGCGGTATCACAATCGCAACTTCTCACGTTGAGTACGATACTCCTGAACGTCACTACGCACACGTA
GACTGTCCTGGACACGCCGATTATGTTAAAAACATGATCACTGGTGCTGCTCAAATGGACGGCGGTATCCTAGTTGTTGC
TGCTACAGATGGCCCTATGCCACAAACTCGTGAGCACATCCTACTTGGTCGTCAAGTTGGTATCCCTTACATCATCGTAT
TCATGAACAAATGTGACATGGTTGATGACGAAGAGCTACTTGAGCTAGTAGAAATGGAAGTTCGTGAACTTCTTTCTGAG
TACGAGTACCCAGGAGACGACCTACCAGTAATTCAAGGTTCTGCACTTGGCGCTCTAAACGGCGAAAAGCAGTGGGAAGA
CAAGATCGTTGAGCTTGCAGAAGCACTAGATTCTTACATTCCACTTCCAGAGCGTGCTGTTGATCTACCGTTCCTACTTC
CTATTGAAGATGTATTCTCAATCCAAGGTCGTGGTACTGTAGTTACTGGTCGTATCGAGCGCGGTATCCTACGTGTAGGT
GACGAAGTAGAAATCGTTGGTATCAAAGAGACTACTCTTACTACTTGTACTGGTGTTGAAATGTTCCGTAAACTGCTTGA
CGAAGGTCGTGCAGGTGAGAACGTTGGTGCACTTCTACGTGGTACTAAGCGTGATGACGTTGAACGTGGCCAAGTACTTT
CTGCTAAAGGTTCAATCAACCCACACACTAAGTTTGAGTCTGAAGTATACGTACTTTCTAAAGACGAAGGCGGCCGTCAC
ACTCCTTTCTTCAAGGGTTACCGTCCACAGTTCTACTTCCGTACAACTGACGTAACAGGCGATATCACTCTACCTGAAGG
CGTAGAAATGGTAATGCCAGGTGACAACGTTCAAATGACTGTTGAGCTAATCGCTCCAATCGCAATGGACGAAGGTCTAC
GTTTCGCAATCCGCGAAGGTGGCCGTACAGTTGGTGCTGGTGTTGTAGCTAAAATCTTCGCTTAA

Upstream 100 bases:

>100_bases
AAATATTGCGCTAACGAGAGTTGGCGCATAAAATAGCAATTTCTGGCGCGTCTCGCTCAATAATGAACGTGGCGAATCAT
AACTAGGAAGGAACACGATT

Downstream 100 bases:

>100_bases
TAGCATTTAGATTTTTGCACACTCTTCATTAGAAGAACTGCATAAGAAAAGGGAAGCTTCGGCTTCCCTTTTATTTTTCC
ACTTTCCCACCACTTCTTTT

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERGITIATSHVEYDTPERHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YEYPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
DEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSINPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Sequences:

>Translated_394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERGITIATSHVEYDTPERHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSE
YEYPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
DEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSINPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
>Mature_393_residues
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERGITIATSHVEYDTPERHYAHVD
CPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEY
EYPGDDLPVIQGSALGALNGEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQGRGTVVTGRIERGILRVGD
EVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSINPHTKFESEVYVLSKDEGGRHT
PFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=55.1898734177215, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.7727272727273, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.7727272727273, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4503475, Length=353, Percent_Identity=32.8611898016997, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=28.47533632287, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI54607086, Length=343, Percent_Identity=27.9883381924198, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018522, Length=331, Percent_Identity=29.607250755287, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI46094014, Length=321, Percent_Identity=29.9065420560748, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4503507, Length=285, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI53729339, Length=231, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI53729337, Length=231, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=32.5757575757576, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=83.756345177665, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=83.969465648855, Blast_Score=658, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=382, Percent_Identity=29.0575916230367, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI48994988, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789108, Length=327, Percent_Identity=29.0519877675841, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.6265664160401, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.702842377261, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=331, Percent_Identity=33.2326283987915, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=331, Percent_Identity=33.2326283987915, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=27.6497695852535, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=313, Percent_Identity=31.3099041533546, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=27.6497695852535, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=26.9121813031161, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17506081, Length=242, Percent_Identity=30.5785123966942, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17509919, Length=441, Percent_Identity=27.437641723356, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=158, Percent_Identity=34.8101265822785, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=28.5106382978723, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=61.7721518987342, Blast_Score=501, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=29.0909090909091, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=29.0909090909091, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=360, Percent_Identity=24.4444444444444, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.1666666666667, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=26.5232974910394, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=29.2181069958848, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=56.0411311053985, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=56.0411311053985, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=51.2886597938144, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=28.1938325991189, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=28.1938325991189, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.1390728476821, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=26.4367816091954, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=29.2753623188406, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647046, Length=278, Percent_Identity=27.6978417266187, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24647048, Length=278, Percent_Identity=27.6978417266187, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28572034, Length=230, Percent_Identity=32.1739130434783, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43233; Mature: 43102

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERG
CCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
ITIATSHVEYDTPERHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYEYPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECHHHHHCCCCCCEEEEEHHHCCCEECC
DEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSIN
CEEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMT
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCEEE
VELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKVYGGVAKDFASIDNAPEERERG
CHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
ITIATSHVEYDTPERHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPYIIVFMNKCDMVDDEELLELVEMEVRELLSEYEYPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEKQWEDKIVELAEALDSYIPLPERAVDLPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECHHHHHCCCCCCEEEEEHHHCCCEECC
DEVEIVGIKETTLTTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLSAKGSIN
CEEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMT
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCEEE
VELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA