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The map label for this gene is fusA1 [H]

Identifier: 218710752

GI number: 218710752

Start: 2982075

End: 2984171

Strand: Reverse

Name: fusA1 [H]

Synonym: VS_2835

Alternate gene names: 218710752

Gene position: 2984171-2982075 (Counterclockwise)

Preceding gene: 218710753

Following gene: 218710751

Centisome position: 90.45

GC content: 44.64

Gene sequence:

>2097_bases
GTGGCTCGTAAAACTCCTATTGAGCAATACCGTAATATCGGTATCGTTGCTCACGTAGATGCAGGTAAAACAACCACAAG
TGAACGTATTCTGTTCTATACCGGTCTTTCTCACAAAATCGGCGAAGTTCACGATGGTGCAGCAACCATGGATTGGATGG
AGCAAGAGCAAGAGCGCGGTATTACGATCACTTCAGCAGCAACTACTACGTTTTGGCGTGGTATGGAAGCTCAGTACTCT
GACCACCGTATTAATATCATCGACACTCCGGGGCACGTTGACTTCACAATCGAAGTAGAACGTTCTCTGCGTGTGCTTGA
TGGTGCAGTTGTTGTGTTCTGTGGCTCATCTGGTGTTGAACCTCAGTCAGAGACTGTATGGCGTCAAGCTGATAAGTACC
AAGTTCCACGTATGGTTTTCGTTAATAAAATGGACCGTACAGGCGCAGACTTCTTGCGTGTAGTTGAACAGATCAAGGAT
CGCCTAGGCGCAACTCCTGTTCCAATTCAACTGAACATTGGTGCTGAAGAAAACTTCCAAGGCGTTGTCGATCTTATCAA
GATGAAGGCGATTAACTGGAATGAAGCTGACCAAGGCATGACTTTCACGTACGAAGATATTCCTGCAGACATGCAAGAAA
TGGCTGAAGAATATCGTACAGAACTTGTTGAAGCTGCTGCAGAAGCAAATGAAGAGCTGATGGATAAGTACCTTGAAGAA
GGTGAACTAACAGAAGCTGAAATCAAACAAGGTCTTCGTACTCGTACCCTTAATAATGAAATCGTACTTGCTACATGTGG
TAGTGCATTCAAAAACAAAGGTGTACAAGCTGTTCTAGATGCAGTTGTTGACTTCCTTCCTTCTCCAGTCGATGTACCTG
CAATTAAAGGTATCGATGAAGACGAGAACGAAGCAGAGCGTCATGCAGACGACAAAGAACCGTTCTCAGCGCTAGCATTT
AAAATTGCAACAGACCCATTTGTTGGTACTTTAACTTTCATCCGTGTTTACTCTGGTGTTGTTGAAAGCGGTAAAACAGC
TTACAACTCTGTGAAGAAACAACGTGAACGTTTAGGGCGCATTGTTCAAATGCACTCAAACAAGCGTGAAGAAGTAAAAG
AAGTACGAGCAGGTGACATCGCAGCCATTATCGGCCTTAAAGATGTTACTACTGGTGAAACTCTATGTGATCAGAACCAT
AAAATTGTTCTTGAACGTATGGAATTCCCAGATCCAGTTATTCAGATCGTTGTAGAGCCTCGCTCTCAAGCTGATCAAGA
TAAAATGACTATCGCGTTAGGTAAGCTAGCGGCAGAAGATCCATCGTTCCGTGTTGAAACGGATGACGAGACTGGCCAGA
CACTAATCTCTGGTATGGGTGAACTGCACTTAGATATCATCGTTGACCGCATGAAGCGTGAATTCAGTGTGAACTGCAAC
GTTGGTAATCCGCAAGTTGCTTATCGTGAAACCATTCGTGGTACAGCGAAAGCTGAAGGTAAATTTATCCGCGAACATGG
TGGTAAAGGACAGTACGGTCACGTGTGGCTGAAACTGGAACCATCAGAAGCCGGTGAAGGCTTTGTCTTCGTAGATGAAA
TTGCCAATGGTATCGTGCCAAAAGAGTTCATTACCTCTGTCGCTAAAGGCGTTGAAGAGCAAATGAACAATGGTGTATTA
GCGGGCTATCCAGTTTTGGATATCAAAGCTACACTATATGATGGTTCTTACCATGAAGTAGATTCAAGTGAGATGGCGTT
TACAATCGCTGCCTCTATGGCTTTCAGAACAGGTGCACTAGAAGCGCAACCAGTTTTGCTTGAGCCTATGATGAAAGTTG
AAGTAACTACTCCAGAAGACTGGATGGGTGACGTTGTTGGCGATATTAACCGTCGTCGCGGCATCATCGAAGGTATGGAC
GAGGGGACAGCTGGCCTGAAGATAATTCGTGCACAAGTTCCGTTGTCTGTCATGTTCGGTTACGCAACTGATTTACGTTC
TGCGACACAAGGTCGTGCTTCTTACTCTATGGAGTTTAGTGAGTACGCTGAAGTGCCAAAAAATGTTGCTAATGCAATCA
TTGCAGAGCGTGGTTAA

Upstream 100 bases:

>100_bases
TTGCTATTTCTAGGGCAAGCACTTTTTAGCGAAGTATCGCTTTTAACGCATAATCTAGACATAGCAATAGTCCCTAAGTC
TCTAATAAGAGGATTCAACC

Downstream 100 bases:

>100_bases
ACAAAAGGGACGCATTTTTTTCATTTTTTGAAAGAACAATGCGTCCAAATATTGCGCTAACGAGAGTTGGCGCATAAAAT
AGCAATTTCTGGCGCGTCTC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 1 [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MARKTPIEQYRNIGIVAHVDAGKTTTSERILFYTGLSHKIGEVHDGAATMDWMEQEQERGITITSAATTTFWRGMEAQYS
DHRINIIDTPGHVDFTIEVERSLRVLDGAVVVFCGSSGVEPQSETVWRQADKYQVPRMVFVNKMDRTGADFLRVVEQIKD
RLGATPVPIQLNIGAEENFQGVVDLIKMKAINWNEADQGMTFTYEDIPADMQEMAEEYRTELVEAAAEANEELMDKYLEE
GELTEAEIKQGLRTRTLNNEIVLATCGSAFKNKGVQAVLDAVVDFLPSPVDVPAIKGIDEDENEAERHADDKEPFSALAF
KIATDPFVGTLTFIRVYSGVVESGKTAYNSVKKQRERLGRIVQMHSNKREEVKEVRAGDIAAIIGLKDVTTGETLCDQNH
KIVLERMEFPDPVIQIVVEPRSQADQDKMTIALGKLAAEDPSFRVETDDETGQTLISGMGELHLDIIVDRMKREFSVNCN
VGNPQVAYRETIRGTAKAEGKFIREHGGKGQYGHVWLKLEPSEAGEGFVFVDEIANGIVPKEFITSVAKGVEEQMNNGVL
AGYPVLDIKATLYDGSYHEVDSSEMAFTIAASMAFRTGALEAQPVLLEPMMKVEVTTPEDWMGDVVGDINRRRGIIEGMD
EGTAGLKIIRAQVPLSVMFGYATDLRSATQGRASYSMEFSEYAEVPKNVANAIIAERG

Sequences:

>Translated_698_residues
MARKTPIEQYRNIGIVAHVDAGKTTTSERILFYTGLSHKIGEVHDGAATMDWMEQEQERGITITSAATTTFWRGMEAQYS
DHRINIIDTPGHVDFTIEVERSLRVLDGAVVVFCGSSGVEPQSETVWRQADKYQVPRMVFVNKMDRTGADFLRVVEQIKD
RLGATPVPIQLNIGAEENFQGVVDLIKMKAINWNEADQGMTFTYEDIPADMQEMAEEYRTELVEAAAEANEELMDKYLEE
GELTEAEIKQGLRTRTLNNEIVLATCGSAFKNKGVQAVLDAVVDFLPSPVDVPAIKGIDEDENEAERHADDKEPFSALAF
KIATDPFVGTLTFIRVYSGVVESGKTAYNSVKKQRERLGRIVQMHSNKREEVKEVRAGDIAAIIGLKDVTTGETLCDQNH
KIVLERMEFPDPVIQIVVEPRSQADQDKMTIALGKLAAEDPSFRVETDDETGQTLISGMGELHLDIIVDRMKREFSVNCN
VGNPQVAYRETIRGTAKAEGKFIREHGGKGQYGHVWLKLEPSEAGEGFVFVDEIANGIVPKEFITSVAKGVEEQMNNGVL
AGYPVLDIKATLYDGSYHEVDSSEMAFTIAASMAFRTGALEAQPVLLEPMMKVEVTTPEDWMGDVVGDINRRRGIIEGMD
EGTAGLKIIRAQVPLSVMFGYATDLRSATQGRASYSMEFSEYAEVPKNVANAIIAERG
>Mature_697_residues
ARKTPIEQYRNIGIVAHVDAGKTTTSERILFYTGLSHKIGEVHDGAATMDWMEQEQERGITITSAATTTFWRGMEAQYSD
HRINIIDTPGHVDFTIEVERSLRVLDGAVVVFCGSSGVEPQSETVWRQADKYQVPRMVFVNKMDRTGADFLRVVEQIKDR
LGATPVPIQLNIGAEENFQGVVDLIKMKAINWNEADQGMTFTYEDIPADMQEMAEEYRTELVEAAAEANEELMDKYLEEG
ELTEAEIKQGLRTRTLNNEIVLATCGSAFKNKGVQAVLDAVVDFLPSPVDVPAIKGIDEDENEAERHADDKEPFSALAFK
IATDPFVGTLTFIRVYSGVVESGKTAYNSVKKQRERLGRIVQMHSNKREEVKEVRAGDIAAIIGLKDVTTGETLCDQNHK
IVLERMEFPDPVIQIVVEPRSQADQDKMTIALGKLAAEDPSFRVETDDETGQTLISGMGELHLDIIVDRMKREFSVNCNV
GNPQVAYRETIRGTAKAEGKFIREHGGKGQYGHVWLKLEPSEAGEGFVFVDEIANGIVPKEFITSVAKGVEEQMNNGVLA
GYPVLDIKATLYDGSYHEVDSSEMAFTIAASMAFRTGALEAQPVLLEPMMKVEVTTPEDWMGDVVGDINRRRGIIEGMDE
GTAGLKIIRAQVPLSVMFGYATDLRSATQGRASYSMEFSEYAEVPKNVANAIIAERG

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=690, Percent_Identity=42.463768115942, Blast_Score=551, Evalue=1e-156,
Organism=Homo sapiens, GI19923640, Length=733, Percent_Identity=37.7899045020464, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI25306287, Length=733, Percent_Identity=36.0163710777626, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI25306283, Length=456, Percent_Identity=41.2280701754386, Blast_Score=314, Evalue=2e-85,
Organism=Homo sapiens, GI4503483, Length=483, Percent_Identity=24.6376811594203, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI157426893, Length=202, Percent_Identity=31.1881188118812, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=39.1304347826087, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=70.5965909090909, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=517, Percent_Identity=25.9187620889749, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI48994988, Length=154, Percent_Identity=40.9090909090909, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=38.6075949367089, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=703, Percent_Identity=39.6870554765292, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17556745, Length=730, Percent_Identity=29.041095890411, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17506493, Length=815, Percent_Identity=26.6257668711656, Blast_Score=187, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17557151, Length=163, Percent_Identity=37.4233128834356, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=147, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=147, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=698, Percent_Identity=41.8338108882521, Blast_Score=548, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6322359, Length=804, Percent_Identity=30.9701492537313, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324707, Length=810, Percent_Identity=25.5555555555556, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320593, Length=810, Percent_Identity=25.5555555555556, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323320, Length=159, Percent_Identity=35.2201257861635, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=174, Percent_Identity=35.0574712643678, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582462, Length=705, Percent_Identity=42.8368794326241, Blast_Score=568, Evalue=1e-162,
Organism=Drosophila melanogaster, GI221458488, Length=727, Percent_Identity=32.5997248968363, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24585709, Length=805, Percent_Identity=25.9627329192547, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24585711, Length=805, Percent_Identity=25.9627329192547, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24585713, Length=805, Percent_Identity=25.9627329192547, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI78706572, Length=202, Percent_Identity=32.1782178217822, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28574573, Length=194, Percent_Identity=33.5051546391753, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357743, Length=136, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77287; Mature: 77156

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKTPIEQYRNIGIVAHVDAGKTTTSERILFYTGLSHKIGEVHDGAATMDWMEQEQERG
CCCCCCHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTTFWRGMEAQYSDHRINIIDTPGHVDFTIEVERSLRVLDGAVVVFCGSSGVE
EEEEECHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCHHHCCCEEEEEECCCCCC
PQSETVWRQADKYQVPRMVFVNKMDRTGADFLRVVEQIKDRLGATPVPIQLNIGAEENFQ
CCHHHHHHHHHHCCCCCEEEHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
GVVDLIKMKAINWNEADQGMTFTYEDIPADMQEMAEEYRTELVEAAAEANEELMDKYLEE
HHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
GELTEAEIKQGLRTRTLNNEIVLATCGSAFKNKGVQAVLDAVVDFLPSPVDVPAIKGIDE
CCCCHHHHHCCHHHEECCCCEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DENEAERHADDKEPFSALAFKIATDPFVGTLTFIRVYSGVVESGKTAYNSVKKQRERLGR
CCHHHHHCCCCCCCHHHHHHHEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IVQMHSNKREEVKEVRAGDIAAIIGLKDVTTGETLCDQNHKIVLERMEFPDPVIQIVVEP
HHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCEEEEEECCCCCCEEEEEECC
RSQADQDKMTIALGKLAAEDPSFRVETDDETGQTLISGMGELHLDIIVDRMKREFSVNCN
CCCCCCHHHEEEEHHHHCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHEEEECC
VGNPQVAYRETIRGTAKAEGKFIREHGGKGQYGHVWLKLEPSEAGEGFVFVDEIANGIVP
CCCCCHHHHHHHCCCHHHCCHHHHHCCCCCCCCEEEEEEECCCCCCCCEEHHHHHCCCCC
KEFITSVAKGVEEQMNNGVLAGYPVLDIKATLYDGSYHEVDSSEMAFTIAASMAFRTGAL
HHHHHHHHHHHHHHHCCCEEECCCEEEEEEEEECCCCCCCCCCCEEEEEHHHHHHHCCCC
EAQPVLLEPMMKVEVTTPEDWMGDVVGDINRRRGIIEGMDEGTAGLKIIRAQVPLSVMFG
CCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHH
YATDLRSATQGRASYSMEFSEYAEVPKNVANAIIAERG
HHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARKTPIEQYRNIGIVAHVDAGKTTTSERILFYTGLSHKIGEVHDGAATMDWMEQEQERG
CCCCCHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTTFWRGMEAQYSDHRINIIDTPGHVDFTIEVERSLRVLDGAVVVFCGSSGVE
EEEEECHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCHHHCCCEEEEEECCCCCC
PQSETVWRQADKYQVPRMVFVNKMDRTGADFLRVVEQIKDRLGATPVPIQLNIGAEENFQ
CCHHHHHHHHHHCCCCCEEEHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
GVVDLIKMKAINWNEADQGMTFTYEDIPADMQEMAEEYRTELVEAAAEANEELMDKYLEE
HHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
GELTEAEIKQGLRTRTLNNEIVLATCGSAFKNKGVQAVLDAVVDFLPSPVDVPAIKGIDE
CCCCHHHHHCCHHHEECCCCEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DENEAERHADDKEPFSALAFKIATDPFVGTLTFIRVYSGVVESGKTAYNSVKKQRERLGR
CCHHHHHCCCCCCCHHHHHHHEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IVQMHSNKREEVKEVRAGDIAAIIGLKDVTTGETLCDQNHKIVLERMEFPDPVIQIVVEP
HHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCEEEEEECCCCCCEEEEEECC
RSQADQDKMTIALGKLAAEDPSFRVETDDETGQTLISGMGELHLDIIVDRMKREFSVNCN
CCCCCCHHHEEEEHHHHCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHEEEECC
VGNPQVAYRETIRGTAKAEGKFIREHGGKGQYGHVWLKLEPSEAGEGFVFVDEIANGIVP
CCCCCHHHHHHHCCCHHHCCHHHHHCCCCCCCCEEEEEEECCCCCCCCEEHHHHHCCCCC
KEFITSVAKGVEEQMNNGVLAGYPVLDIKATLYDGSYHEVDSSEMAFTIAASMAFRTGAL
HHHHHHHHHHHHHHHCCCEEECCCEEEEEEEEECCCCCCCCCCCEEEEEHHHHHHHCCCC
EAQPVLLEPMMKVEVTTPEDWMGDVVGDINRRRGIIEGMDEGTAGLKIIRAQVPLSVMFG
CCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHH
YATDLRSATQGRASYSMEFSEYAEVPKNVANAIIAERG
HHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]