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The map label for this gene is pyrB [H]

Identifier: 218710660

GI number: 218710660

Start: 2908847

End: 2909776

Strand: Direct

Name: pyrB [H]

Synonym: VS_2740

Alternate gene names: 218710660

Gene position: 2908847-2909776 (Clockwise)

Preceding gene: 218710659

Following gene: 218710661

Centisome position: 88.17

GC content: 46.77

Gene sequence:

>930_bases
ATGGCGAATTCGCTCTATCAAAAGCACATCATCTCAATTCCAGAGCTTTCTCGTGAAGAGCTAGAATTAATTGTTCAAAC
GGCAGGTCAACTTAAAGCTGAACCAAACCCAGAACTCATCAAGAACAAAGTTGTTGCCAGCTGCTTCTTCGAACCTTCAA
CACGAACTCGTCTCTCTTTTGAAACGGCTATTCAGCGCATAGGTGGTGATGTGATTGGTTTCGACAGTGGTGGTAACACG
TCACTAGCGAAGAAAGGCGAAACGCTAGCGGACTCAGTACAGGTTATCTCTTCATACGTTGATGCTTACGTAATGCGCCA
CCCTCAAGAAGGCGCAGCGCGTCTAGCTTCTGAGTTCTCTAACGGTGTACCTGTTATTAACGCGGGCGACGGAGCAAACC
AACACCCAACACAAACGTTATTAGACCTGTTCTCTATCGCCGAAACTCAAGGCCGCCTCGACAACCTTAACGTTGCATTC
GTTGGTGACCTTAAGTACGGCCGTACAGTTCACTCGCTAACTCAAGCACTAGCGAAGTTCGACAACATCTGTTTCTACTT
CGTTGCTCCAGAAGCACTGGCGATGCCAGACTACATTTGCGAAGAACTTGATGAAGCGGGTATCAAGTACCAACTTCTGA
CAGACATGGAAGATGTGATTCCAGAGTTGGATGTTCTGTACATGACTCGAGTTCAAAAAGAGCGCTTCGATGAATCGGAA
TACGCGCACATCAAATCAGCGTACATCCTGACTGCTTCGATGCTGGAAAATGCACGTGATAACCTAAAGGTTCTTCACCC
TCTTCCTCGTGTTGATGAAATTACTGTCGATGTCGATAAAACACCTTACGCTTACTACTTCCAGCAAGCAGAGAACGGTG
TTTACGCACGTGAAGCACTGCTAGCCCTTGTTCTTAACGAAACGCTGTAG

Upstream 100 bases:

>100_bases
TGCTTGCCAGGCCGTCTATTCTGCTTTTCAGCACTTTTTTAAAGCCTCCCATTATGGGGGGCTTTTTTATGGCCAATACA
TTATTGTGGGGAAGAAGATC

Downstream 100 bases:

>100_bases
AGGAGAGATATCATGTCTAAAGAGACTCAATTAAAAGTTGAAGCAATCAAGAACGGAACGGTTATCGACCATATCCCAGC
CAACATCGGGATCAAGGTAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MANSLYQKHIISIPELSREELELIVQTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGDVIGFDSGGNT
SLAKKGETLADSVQVISSYVDAYVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTLLDLFSIAETQGRLDNLNVAF
VGDLKYGRTVHSLTQALAKFDNICFYFVAPEALAMPDYICEELDEAGIKYQLLTDMEDVIPELDVLYMTRVQKERFDESE
YAHIKSAYILTASMLENARDNLKVLHPLPRVDEITVDVDKTPYAYYFQQAENGVYAREALLALVLNETL

Sequences:

>Translated_309_residues
MANSLYQKHIISIPELSREELELIVQTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGDVIGFDSGGNT
SLAKKGETLADSVQVISSYVDAYVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTLLDLFSIAETQGRLDNLNVAF
VGDLKYGRTVHSLTQALAKFDNICFYFVAPEALAMPDYICEELDEAGIKYQLLTDMEDVIPELDVLYMTRVQKERFDESE
YAHIKSAYILTASMLENARDNLKVLHPLPRVDEITVDVDKTPYAYYFQQAENGVYAREALLALVLNETL
>Mature_308_residues
ANSLYQKHIISIPELSREELELIVQTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGDVIGFDSGGNTS
LAKKGETLADSVQVISSYVDAYVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTLLDLFSIAETQGRLDNLNVAFV
GDLKYGRTVHSLTQALAKFDNICFYFVAPEALAMPDYICEELDEAGIKYQLLTDMEDVIPELDVLYMTRVQKERFDESEY
AHIKSAYILTASMLENARDNLKVLHPLPRVDEITVDVDKTPYAYYFQQAENGVYAREALLALVLNETL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=44.3729903536978, Blast_Score=264, Evalue=7e-71,
Organism=Homo sapiens, GI38788445, Length=319, Percent_Identity=26.3322884012539, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=71.1974110032362, Blast_Score=473, Evalue=1e-135,
Organism=Escherichia coli, GI1786469, Length=343, Percent_Identity=26.8221574344023, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790703, Length=340, Percent_Identity=23.8235294117647, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=40.1840490797546, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=41.1003236245955, Blast_Score=238, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6322373, Length=326, Percent_Identity=24.8466257668712, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=45.5128205128205, Blast_Score=264, Evalue=6e-71,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34378; Mature: 34247

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSLYQKHIISIPELSREELELIVQTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSF
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
ETAIQRIGGDVIGFDSGGNTSLAKKGETLADSVQVISSYVDAYVMRHPQEGAARLASEFS
HHHHHHHCCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGVPVINAGDGANQHPTQTLLDLFSIAETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
DNICFYFVAPEALAMPDYICEELDEAGIKYQLLTDMEDVIPELDVLYMTRVQKERFDESE
CCEEEEEECCHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YAHIKSAYILTASMLENARDNLKVLHPLPRVDEITVDVDKTPYAYYFQQAENGVYAREAL
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
LALVLNETL
HHHHHHHCC
>Mature Secondary Structure 
ANSLYQKHIISIPELSREELELIVQTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSF
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
ETAIQRIGGDVIGFDSGGNTSLAKKGETLADSVQVISSYVDAYVMRHPQEGAARLASEFS
HHHHHHHCCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGVPVINAGDGANQHPTQTLLDLFSIAETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
DNICFYFVAPEALAMPDYICEELDEAGIKYQLLTDMEDVIPELDVLYMTRVQKERFDESE
CCEEEEEECCHHHCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YAHIKSAYILTASMLENARDNLKVLHPLPRVDEITVDVDKTPYAYYFQQAENGVYAREAL
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHH
LALVLNETL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA