Click here to switch to the map view.

The map label for this gene is argF [H]

Identifier: 218710659

GI number: 218710659

Start: 2907555

End: 2908559

Strand: Direct

Name: argF [H]

Synonym: VS_2739

Alternate gene names: 218710659

Gene position: 2907555-2908559 (Clockwise)

Preceding gene: 218710658

Following gene: 218710660

Centisome position: 88.13

GC content: 45.57

Gene sequence:

>1005_bases
ATGGCATTTAATCTTCGCAATCGTAACTTTCTAAAACTTCTCGACTTTACTCCTAAAGAGATTCAGTTTTTACTCGACCT
GTCTGCTGACCTTAAAAAGGCTAAGTATGCAGGTACAGAGCAAAAGAAGCTTAACGGTAAAAACATCGCTTTGATCTTTG
AAAAAGCATCCACTCGAACTCGATGTGCTTTTGAGGTAGCTGCTTTTGATCAAGGCGCTCAAGTCTCTTATTTAGGCCCT
TCTGGTTCTCAGATTGGTCAGAAAGAATCAATGAAAGATACGGCGCGTGTATTAGGTCGCATGTACGATGGCATTGAATA
CCGAGGGTTTGGCCAAAGCATTGTCGAAGACCTAGGCGCTTATGCTGGTGTACCGGTTTGGAATGGCTTAACCGATGAGT
TCCACCCTACTCAGATCTTGGCTGATTTCCTCACTATGCTTGAGCATGGCCGAGGAAAGCATCTGCACCAAATCAGCTTT
GCTTACCTTGGTGATGCACGTAATAACATGGGTAACTCTCTGTTAGTGGGTGCCGCGAAAATGGGCATGGATATTCGCCT
TGTCGCGCCAAAAGCATTTTGGCCAGAAGAGCACCTTGTCGAAGAGTGCCAAGCTATCGCGCAAAGTACAGGTGCAAAAA
TCACGCTGACAGAAGACGTTGCCGAAGGTGTGAAAGGCTGTGATTTCCTTTATACCGATGTTTGGGTTTCTATGGGTGAA
GCGCCAGAAGCTTGGGACGAACGTGTTGCTGTAATGACACCTTACCAAGTGAATATGGATGTCATAAAGCTCACAGGTAA
CCCACAAGTGAAGTTCATGCATTGCCTGCCAGCTTTCCACAACAATGAAACCGTGATCGGCCAGCAAGTCGCAGACAAAT
ATGGAATGAACGGTTTGGAAGTAACAGACGAAGTATTTGAGTCCGACTACTCAATTGTATTTGATGAAGCAGAGAATCGC
ATGCACACCATCAAAGCAGTAATGGTCGCGACACTTGGCCAATAG

Upstream 100 bases:

>100_bases
TGGCCTTTTTTATCAAATTAACAAAATAAATATTCACAAATGTAGCATTTTTATGTTTAACTAAGTTCTTCATTGATTCT
ATATACCCAAGGAGCGAGAG

Downstream 100 bases:

>100_bases
AGAATAATGAAAGTAAACAAAAGCTTGATGTAATCGCTTGCGCTCACAAATTGAAAGCGTATAATTCTCGGCAATTTGTC
TGAGAGTAGTGAAAATGACG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAFNLRNRNFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQKKLNGKNIALIFEKASTRTRCAFEVAAFDQGAQVSYLGP
SGSQIGQKESMKDTARVLGRMYDGIEYRGFGQSIVEDLGAYAGVPVWNGLTDEFHPTQILADFLTMLEHGRGKHLHQISF
AYLGDARNNMGNSLLVGAAKMGMDIRLVAPKAFWPEEHLVEECQAIAQSTGAKITLTEDVAEGVKGCDFLYTDVWVSMGE
APEAWDERVAVMTPYQVNMDVIKLTGNPQVKFMHCLPAFHNNETVIGQQVADKYGMNGLEVTDEVFESDYSIVFDEAENR
MHTIKAVMVATLGQ

Sequences:

>Translated_334_residues
MAFNLRNRNFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQKKLNGKNIALIFEKASTRTRCAFEVAAFDQGAQVSYLGP
SGSQIGQKESMKDTARVLGRMYDGIEYRGFGQSIVEDLGAYAGVPVWNGLTDEFHPTQILADFLTMLEHGRGKHLHQISF
AYLGDARNNMGNSLLVGAAKMGMDIRLVAPKAFWPEEHLVEECQAIAQSTGAKITLTEDVAEGVKGCDFLYTDVWVSMGE
APEAWDERVAVMTPYQVNMDVIKLTGNPQVKFMHCLPAFHNNETVIGQQVADKYGMNGLEVTDEVFESDYSIVFDEAENR
MHTIKAVMVATLGQ
>Mature_333_residues
AFNLRNRNFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQKKLNGKNIALIFEKASTRTRCAFEVAAFDQGAQVSYLGPS
GSQIGQKESMKDTARVLGRMYDGIEYRGFGQSIVEDLGAYAGVPVWNGLTDEFHPTQILADFLTMLEHGRGKHLHQISFA
YLGDARNNMGNSLLVGAAKMGMDIRLVAPKAFWPEEHLVEECQAIAQSTGAKITLTEDVAEGVKGCDFLYTDVWVSMGEA
PEAWDERVAVMTPYQVNMDVIKLTGNPQVKFMHCLPAFHNNETVIGQQVADKYGMNGLEVTDEVFESDYSIVFDEAENRM
HTIKAVMVATLGQ

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=329, Percent_Identity=38.6018237082067, Blast_Score=210, Evalue=1e-54,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=68.3890577507599, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=68.0722891566265, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI48994908, Length=376, Percent_Identity=24.7340425531915, Blast_Score=78, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=332, Percent_Identity=31.9277108433735, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 37096; Mature: 36965

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNLRNRNFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQKKLNGKNIALIFEKASTRT
CEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCC
RCAFEVAAFDQGAQVSYLGPSGSQIGQKESMKDTARVLGRMYDGIEYRGFGQSIVEDLGA
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
YAGVPVWNGLTDEFHPTQILADFLTMLEHGRGKHLHQISFAYLGDARNNMGNSLLVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHEECCHHCCCCCEEEEEHHH
MGMDIRLVAPKAFWPEEHLVEECQAIAQSTGAKITLTEDVAEGVKGCDFLYTDVWVSMGE
HCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCC
APEAWDERVAVMTPYQVNMDVIKLTGNPQVKFMHCLPAFHNNETVIGQQVADKYGMNGLE
CCCHHHCCEEEECCEEECEEEEEECCCCCEEEEEEECCCCCCCEEHHHHHHHHHCCCCCC
VTDEVFESDYSIVFDEAENRMHTIKAVMVATLGQ
CCHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFNLRNRNFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQKKLNGKNIALIFEKASTRT
EECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCC
RCAFEVAAFDQGAQVSYLGPSGSQIGQKESMKDTARVLGRMYDGIEYRGFGQSIVEDLGA
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHH
YAGVPVWNGLTDEFHPTQILADFLTMLEHGRGKHLHQISFAYLGDARNNMGNSLLVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHEECCHHCCCCCEEEEEHHH
MGMDIRLVAPKAFWPEEHLVEECQAIAQSTGAKITLTEDVAEGVKGCDFLYTDVWVSMGE
HCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCC
APEAWDERVAVMTPYQVNMDVIKLTGNPQVKFMHCLPAFHNNETVIGQQVADKYGMNGLE
CCCHHHCCEEEECCEEECEEEEEECCCCCEEEEEEECCCCCCCEEHHHHHHHHHCCCCCC
VTDEVFESDYSIVFDEAENRMHTIKAVMVATLGQ
CCHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA