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The map label for this gene is ygfZ [C]

Identifier: 218710580

GI number: 218710580

Start: 2818019

End: 2818990

Strand: Direct

Name: ygfZ [C]

Synonym: VS_2630

Alternate gene names: 218710580

Gene position: 2818019-2818990 (Clockwise)

Preceding gene: 218710577

Following gene: 218710581

Centisome position: 85.41

GC content: 46.09

Gene sequence:

>972_bases
ATGGATTGGAAAAACACATTTCAGCCGCTCGCTCATACGCAAAATGAATCGCTTCCAGAACTGATGATGACACACGTGTC
AGATTGGAGCGCAATTACCATGATAGGCGATGACAAAAAGTCGTACCTACAAGGTCAAGTTACGTGCGATGTCGTCACTC
TTCCTAATGATGAATCTACATTAGGTGCGCATTGTGATGCGAAAGGAAAGGTATGGAGTATCTTTCGCTTGTTCCACCAC
AACGGTGGCTACGCACTCATGCAGCCTAAGTCTGCGATTGAAGTCGAATTAGTTGAAATCAAGAAATACGCTGTATTCTC
TAAGGTTGATATCGAGCAAACGTCTGACGTTGTTATCGGTGTCATGGGCGCGTCCGCTGATCAATACATTGACTCGATTT
CAGAAAGCCAAGGTAAAGTGCGTGTAATCTCTGGTGGCACCGCTGTTCAAGTCTCTGACAACCGCTGGGCTCTACTTGTT
ACGCAAGAAGCAACAGAAGCCTTGGTATCAAGCAGCACTGCAGAAAAAGTATCAGAAGCGCTTTGGCAGTATCATGAAAT
TCTTGATGCTCAGCCGAACCTATCGAAAGCAGAGCAAAACGAACACATCCCTCAAGCACTCAACCTGCAAGCGATTGGCG
GTATCAGCTTTTCAAAAGGCTGTTACACAGGTCAAGAAACAGTTGCTCGTGCTAAATACCGTGGCATGAACAAGCGTGAA
ATGCGCATTGTTTCAGGAACGAGTTCAGATGTATTGTCTCTAGAGAATACGATTGAACTAGAGCGCAGCGTAGGTGAGAA
CTGGCGCGGTGCAGGCCGACTATTAAACGTCTATCAATTTGCTGATAATCAGGCGATTGGTTTGATGGTGTTGCCAAACA
ACCTTGATGACGATGTTCAGCTTCGATTGACTGCGCAACCTGAGCAAATATGGAACATTCTGCCACTGCCTTACAGCCTT
GACGAAGAGTAG

Upstream 100 bases:

>100_bases
TGAGTCACAGTCCCTATCTAAGCTCACAAAAAAACCTAAGCTGACAAAGAAACAGAACCGGATTAACATAGAGCCAAATA
AAATTCTTAGGAAAGATAAA

Downstream 100 bases:

>100_bases
CCACGTGGAAACACTGATTACACAATGGTTAGATCAACAGCAGGTGGACTATCGCCTGTTGGTGCAAAGCAAGCCAACCA
CCAGCATCGAAGAGACAGCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDWKNTFQPLAHTQNESLPELMMTHVSDWSAITMIGDDKKSYLQGQVTCDVVTLPNDESTLGAHCDAKGKVWSIFRLFHH
NGGYALMQPKSAIEVELVEIKKYAVFSKVDIEQTSDVVIGVMGASADQYIDSISESQGKVRVISGGTAVQVSDNRWALLV
TQEATEALVSSSTAEKVSEALWQYHEILDAQPNLSKAEQNEHIPQALNLQAIGGISFSKGCYTGQETVARAKYRGMNKRE
MRIVSGTSSDVLSLENTIELERSVGENWRGAGRLLNVYQFADNQAIGLMVLPNNLDDDVQLRLTAQPEQIWNILPLPYSL
DEE

Sequences:

>Translated_323_residues
MDWKNTFQPLAHTQNESLPELMMTHVSDWSAITMIGDDKKSYLQGQVTCDVVTLPNDESTLGAHCDAKGKVWSIFRLFHH
NGGYALMQPKSAIEVELVEIKKYAVFSKVDIEQTSDVVIGVMGASADQYIDSISESQGKVRVISGGTAVQVSDNRWALLV
TQEATEALVSSSTAEKVSEALWQYHEILDAQPNLSKAEQNEHIPQALNLQAIGGISFSKGCYTGQETVARAKYRGMNKRE
MRIVSGTSSDVLSLENTIELERSVGENWRGAGRLLNVYQFADNQAIGLMVLPNNLDDDVQLRLTAQPEQIWNILPLPYSL
DEE
>Mature_323_residues
MDWKNTFQPLAHTQNESLPELMMTHVSDWSAITMIGDDKKSYLQGQVTCDVVTLPNDESTLGAHCDAKGKVWSIFRLFHH
NGGYALMQPKSAIEVELVEIKKYAVFSKVDIEQTSDVVIGVMGASADQYIDSISESQGKVRVISGGTAVQVSDNRWALLV
TQEATEALVSSSTAEKVSEALWQYHEILDAQPNLSKAEQNEHIPQALNLQAIGGISFSKGCYTGQETVARAKYRGMNKRE
MRIVSGTSSDVLSLENTIELERSVGENWRGAGRLLNVYQFADNQAIGLMVLPNNLDDDVQLRLTAQPEQIWNILPLPYSL
DEE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=326, Percent_Identity=40.7975460122699, Blast_Score=208, Evalue=4e-55,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_VIBSL (B7VK90)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418201.1
- GeneID:   7162149
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2630
- HOGENOM:   HBG700628
- OMA:   GAHCDPK
- ProtClustDB:   CLSK874810
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T

EC number: NA

Molecular weight: Translated: 35792; Mature: 35792

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWKNTFQPLAHTQNESLPELMMTHVSDWSAITMIGDDKKSYLQGQVTCDVVTLPNDEST
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCEEEEEEEECCCCCCC
LGAHCDAKGKVWSIFRLFHHNGGYALMQPKSAIEVELVEIKKYAVFSKVDIEQTSDVVIG
CCCCCCCCCCHHHEEEEHHCCCCEEEECCCCCCEEEEEEEHHHHEEEECCCCCCCCEEEE
VMGASADQYIDSISESQGKVRVISGGTAVQVSDNRWALLVTQEATEALVSSSTAEKVSEA
EECCCHHHHHHHHHCCCCCEEEEECCEEEEECCCCEEEEEECHHHHHHHHCCHHHHHHHH
LWQYHEILDAQPNLSKAEQNEHIPQALNLQAIGGISFSKGCYTGQETVARAKYRGMNKRE
HHHHHHHHCCCCCCCHHHHCCCCCHHCCEEEECCCEECCCCCCCHHHHHHHHHCCCCCCE
MRIVSGTSSDVLSLENTIELERSVGENWRGAGRLLNVYQFADNQAIGLMVLPNNLDDDVQ
EEEEECCCCCEEEHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCEEEEEEECCCCCCCEE
LRLTAQPEQIWNILPLPYSLDEE
EEEEECHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MDWKNTFQPLAHTQNESLPELMMTHVSDWSAITMIGDDKKSYLQGQVTCDVVTLPNDEST
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCEEEEEEEECCCCCCC
LGAHCDAKGKVWSIFRLFHHNGGYALMQPKSAIEVELVEIKKYAVFSKVDIEQTSDVVIG
CCCCCCCCCCHHHEEEEHHCCCCEEEECCCCCCEEEEEEEHHHHEEEECCCCCCCCEEEE
VMGASADQYIDSISESQGKVRVISGGTAVQVSDNRWALLVTQEATEALVSSSTAEKVSEA
EECCCHHHHHHHHHCCCCCEEEEECCEEEEECCCCEEEEEECHHHHHHHHCCHHHHHHHH
LWQYHEILDAQPNLSKAEQNEHIPQALNLQAIGGISFSKGCYTGQETVARAKYRGMNKRE
HHHHHHHHCCCCCCCHHHHCCCCCHHCCEEEECCCEECCCCCCCHHHHHHHHHCCCCCCE
MRIVSGTSSDVLSLENTIELERSVGENWRGAGRLLNVYQFADNQAIGLMVLPNNLDDDVQ
EEEEECCCCCEEEHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCEEEEEEECCCCCCCEE
LRLTAQPEQIWNILPLPYSLDEE
EEEEECHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA