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The map label for this gene is nadB [H]

Identifier: 218710577

GI number: 218710577

Start: 2815521

End: 2817137

Strand: Direct

Name: nadB [H]

Synonym: VS_2627

Alternate gene names: 218710577

Gene position: 2815521-2817137 (Clockwise)

Preceding gene: 218710564

Following gene: 218710580

Centisome position: 85.34

GC content: 47.25

Gene sequence:

>1617_bases
ATGAACGCAAACCGTGAACATCAGTGTGATGTATTAGTGGTAGGAAGTGGTGCGGCAGGCTTGTCATTAGCCTTACGCGT
AGCAGAACATGCAAAAGTAATTGTATTAAGCAAAGGACCACGCAGCGAAGGATCGACGTATTACGCACAAGGTGGTATTG
CGGCGGTGTTCGATGAATCGGACAGTATTGAGTCTCATGTAGAAGATACTCAAATTGCTGGGGCTGGGTTATGCGAAGAA
GATACAGTTCAATTCATTGCTGAAAATGCTAAAGAGTGTGTGCAATGGCTGATTGATGGTGGTGTTCCATTTGATAAAGA
TGAGAACAGCACAGAAGGCCAACCAAAGTATCACCTCACTCGTGAGGGCGGCCACAGTCACCGCAGAATTCTGCACGCTG
CCGATGCAACTGGCATGGCGATGCAAACCTCACTGCAAGATAACGTCAAGAATCACCCAAACATCGAGATCTTCGAGCGT
CACAATGCGCTGGATTTAATCACCGAAGATAAGATAGGTGGTTCGAAAGACAAGGTTATCGGTGCCTATATTTGGAACCG
TAACCAAGAACACGTTGAAACCGTGCGCGCTAAATTTGTTGTATTAGCAACAGGCGGCGCTTCAAAGGTTTACCAATACA
CCTCTAACCCAGATGTCTCTTCAGGTGATGGTATTGCTATCGCTTGGCGTGCGGGTTGTCGTGTCGCAAACCTTGAGTTC
AACCAGTTCCACCCAACTTGCTTATTCCACCCAGAAGCACGTAACTTCCTACTAACGGAAGCATTGCGTGGTGAAGGTGC
CTATTTACGTCGCCCAGATGGCTCTCGCTTTATGAAGGACTTCGATGAGCGCGGTGAATTGGCTCCACGTGATGTGGTTG
CTCGTGCGATTGACTTTGAAATGAAGCGCTTAGGTGCTGACTGCATGTATGTTGATATCAGCCACAAACCTGAAGAATTC
ATCACGACGCATTTCCCAATGATCCATACTCGCTTGATGGATTTGGGGATTGATATGACCAAAGAACCGATTCCAATCGT
ACCGGCAGCTCACTACACTTGTGGTGGTGTCATGGTGAATAAGCAAGGTCAAACCGACTTAACTAACCTATATGCAATTG
GCGAAGTGAGTTATACCGGCTTGCACGGTGCAAACCGTATGGCTTCAAACTCACTGCTTGAGTGTGTGGTATACGCGTGG
GCAGCCGCAAAAGATATCGTTGAAAACATCGACCAATCTCAACTGTGCGCTGAACTTCCGGCATGGGATGAAAGCCAAGT
CACCAACAGTGATGAAGAAGTGATCATTCAACACAACTGGCACGAGCTAAGACTGTTCATGTGGGATTACATGGGTATTG
TTCGAACGGACAAACGCCTTGAACGTGCAATGCGCCGCATTCAGATGTTGCAGCAGGAAACTCATGAGTACTACAGCTAC
TTTAAGGTTTCAAATAACCTATTAGAACTGCGTAACTTACTACAAGTGGCTGAGTTGATGGTTCGCTGTGCAATGCAACG
TAAAGAGAGCCGCGGCCTACACTACACGCTGGATTACCCAGAACTTGCTGAAGACAGCGGCCCAACCATTCTGACACCAG
AGAAAAACCAGTCTTAA

Upstream 100 bases:

>100_bases
ACTAGGGAAAGATTATAACAGTCTTACCCAAACTCCTAAAACAAGACGAAGTCAATTGAGCATGGACAACTCGACTATAG
CCCGGGATTTAATAAGTTTT

Downstream 100 bases:

>100_bases
TTTTTGGTCATGGAAAAAGACGACAGGGAGCCTAGCGCTCCCTTTCTTTTATCTTTGGTTCTTGTTTATCCAATGCTTTC
GTTTATCAAACGGACTCGTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MNANREHQCDVLVVGSGAAGLSLALRVAEHAKVIVLSKGPRSEGSTYYAQGGIAAVFDESDSIESHVEDTQIAGAGLCEE
DTVQFIAENAKECVQWLIDGGVPFDKDENSTEGQPKYHLTREGGHSHRRILHAADATGMAMQTSLQDNVKNHPNIEIFER
HNALDLITEDKIGGSKDKVIGAYIWNRNQEHVETVRAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANLEF
NQFHPTCLFHPEARNFLLTEALRGEGAYLRRPDGSRFMKDFDERGELAPRDVVARAIDFEMKRLGADCMYVDISHKPEEF
ITTHFPMIHTRLMDLGIDMTKEPIPIVPAAHYTCGGVMVNKQGQTDLTNLYAIGEVSYTGLHGANRMASNSLLECVVYAW
AAAKDIVENIDQSQLCAELPAWDESQVTNSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERAMRRIQMLQQETHEYYSY
FKVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPELAEDSGPTILTPEKNQS

Sequences:

>Translated_538_residues
MNANREHQCDVLVVGSGAAGLSLALRVAEHAKVIVLSKGPRSEGSTYYAQGGIAAVFDESDSIESHVEDTQIAGAGLCEE
DTVQFIAENAKECVQWLIDGGVPFDKDENSTEGQPKYHLTREGGHSHRRILHAADATGMAMQTSLQDNVKNHPNIEIFER
HNALDLITEDKIGGSKDKVIGAYIWNRNQEHVETVRAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANLEF
NQFHPTCLFHPEARNFLLTEALRGEGAYLRRPDGSRFMKDFDERGELAPRDVVARAIDFEMKRLGADCMYVDISHKPEEF
ITTHFPMIHTRLMDLGIDMTKEPIPIVPAAHYTCGGVMVNKQGQTDLTNLYAIGEVSYTGLHGANRMASNSLLECVVYAW
AAAKDIVENIDQSQLCAELPAWDESQVTNSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERAMRRIQMLQQETHEYYSY
FKVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPELAEDSGPTILTPEKNQS
>Mature_538_residues
MNANREHQCDVLVVGSGAAGLSLALRVAEHAKVIVLSKGPRSEGSTYYAQGGIAAVFDESDSIESHVEDTQIAGAGLCEE
DTVQFIAENAKECVQWLIDGGVPFDKDENSTEGQPKYHLTREGGHSHRRILHAADATGMAMQTSLQDNVKNHPNIEIFER
HNALDLITEDKIGGSKDKVIGAYIWNRNQEHVETVRAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANLEF
NQFHPTCLFHPEARNFLLTEALRGEGAYLRRPDGSRFMKDFDERGELAPRDVVARAIDFEMKRLGADCMYVDISHKPEEF
ITTHFPMIHTRLMDLGIDMTKEPIPIVPAAHYTCGGVMVNKQGQTDLTNLYAIGEVSYTGLHGANRMASNSLLECVVYAW
AAAKDIVENIDQSQLCAELPAWDESQVTNSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERAMRRIQMLQQETHEYYSY
FKVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPELAEDSGPTILTPEKNQS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=510, Percent_Identity=32.7450980392157, Blast_Score=203, Evalue=4e-52,
Organism=Escherichia coli, GI1788928, Length=535, Percent_Identity=73.4579439252336, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=528, Percent_Identity=31.6287878787879, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786942, Length=558, Percent_Identity=30.4659498207885, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17550100, Length=533, Percent_Identity=30.9568480300188, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=527, Percent_Identity=30.550284629981, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI71986328, Length=283, Percent_Identity=26.1484098939929, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322701, Length=552, Percent_Identity=31.5217391304348, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=555, Percent_Identity=31.5315315315315, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6320788, Length=487, Percent_Identity=25.6673511293634, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17137288, Length=536, Percent_Identity=31.3432835820896, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655642, Length=536, Percent_Identity=31.3432835820896, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655647, Length=536, Percent_Identity=31.3432835820896, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24663005, Length=557, Percent_Identity=27.6481149012567, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60379; Mature: 60379

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANREHQCDVLVVGSGAAGLSLALRVAEHAKVIVLSKGPRSEGSTYYAQGGIAAVFDES
CCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCC
DSIESHVEDTQIAGAGLCEEDTVQFIAENAKECVQWLIDGGVPFDKDENSTEGQPKYHLT
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
REGGHSHRRILHAADATGMAMQTSLQDNVKNHPNIEIFERHNALDLITEDKIGGSKDKVI
CCCCCCHHHEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCEE
GAYIWNRNQEHVETVRAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANLEF
EEEEECCCHHHHHHHHEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCEEEEEEC
NQFHPTCLFHPEARNFLLTEALRGEGAYLRRPDGSRFMKDFDERGELAPRDVVARAIDFE
CCCCCEEEECCCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
MKRLGADCMYVDISHKPEEFITTHFPMIHTRLMDLGIDMTKEPIPIVPAAHYTCGGVMVN
HHHCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEC
KQGQTDLTNLYAIGEVSYTGLHGANRMASNSLLECVVYAWAAAKDIVENIDQSQLCAELP
CCCCCCHHHEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
AWDESQVTNSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERAMRRIQMLQQETHEYYSY
CCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPELAEDSGPTILTPEKNQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MNANREHQCDVLVVGSGAAGLSLALRVAEHAKVIVLSKGPRSEGSTYYAQGGIAAVFDES
CCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCC
DSIESHVEDTQIAGAGLCEEDTVQFIAENAKECVQWLIDGGVPFDKDENSTEGQPKYHLT
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
REGGHSHRRILHAADATGMAMQTSLQDNVKNHPNIEIFERHNALDLITEDKIGGSKDKVI
CCCCCCHHHEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCEE
GAYIWNRNQEHVETVRAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANLEF
EEEEECCCHHHHHHHHEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCEEEEEEC
NQFHPTCLFHPEARNFLLTEALRGEGAYLRRPDGSRFMKDFDERGELAPRDVVARAIDFE
CCCCCEEEECCCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
MKRLGADCMYVDISHKPEEFITTHFPMIHTRLMDLGIDMTKEPIPIVPAAHYTCGGVMVN
HHHCCCCEEEEECCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEC
KQGQTDLTNLYAIGEVSYTGLHGANRMASNSLLECVVYAWAAAKDIVENIDQSQLCAELP
CCCCCCHHHEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
AWDESQVTNSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERAMRRIQMLQQETHEYYSY
CCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPELAEDSGPTILTPEKNQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]