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The map label for this gene is rumA

Identifier: 218710562

GI number: 218710562

Start: 2801263

End: 2802591

Strand: Reverse

Name: rumA

Synonym: VS_2612

Alternate gene names: 218710562

Gene position: 2802591-2801263 (Counterclockwise)

Preceding gene: 218710565

Following gene: 218710561

Centisome position: 84.94

GC content: 44.92

Gene sequence:

>1329_bases
ATGGCACGTTTTTTCCAACCAAAAAAGAAAACTCAACTCGAGACCAAGCATCAATCGGTTTTGGTTGAACGAATGGATCA
CAATGGCGCTGGCATCGCTTATCAGAAGAACAAACCCGTTTTCATTGATGGCGCATTGCCCGGTGAGAAGGTGGTTATTC
AACTTACTGAGAGCAAAAGTAAGTTTTCGCGAGCAAAACTTATTAAATTACTTCAACCGAGTGAGCTGCGTTTAAAGCCA
TTTTGTAAGCACTTTAATCAGTGTGGTGGCTGTCATCTCCAACATCTCGGTTACTCATCTCAGGTTGAGCATAAATCACA
ATCTTTGACGCACCTGATGAGCAAATACCAAACGGCTAACACCGAAGTCGCCCTGCCGATTGTCGGTGATGAAAGCGGTT
ATCGTCGTCGCGCGCGTATCAGTCTATTTGTTGATAAGAAGACACAGCTGCTTCAGTTTGGTTTCCGTAAGAAACAAAGT
AAACAGATTGAAAACATCACCGACTGCGCGGTGCTTGACCCTCGCCTTAATGTATTGCTGCCAAAACTGAAAAGTTTACT
GAACACTTTCAGTAGCCTTACGTCTTTAGGACATGTTGAATTGGTGTTGGGTGATACTGGTCCTGTTATGGTGCTGCGTC
ACCTTAAACCTTTGGTTGAAAAAGATGAGCAAGCGTTGATGACGCTGATGACGCTCGCGAAAGAAGAAGGTGCAACGCTG
TACTCGATGCCTGAAACCGATAAGTTGGTCCGTCTGGTTGGTGATGCGCCTAGCTACAGCGAAACAGGTGTTACCTTACC
TTTTGAGCCAAACCACTTTATCCAAGTCAATCAGAAAGTGAATCAGCAGATGGTGGCTCAAGCGATTGAGTGGTTAGAGC
CTCAAGCGGATGAGCGTGTGCTTGACCTGTTTTGTGGTTTAGGCAACTTCAGTCTGCCTATCGCACAACAATCGGCGTTT
GTCGTTGGTGTGGAAGGCGTTGATGAAATGGTTCAACAGGCAACGGCTAATGCGGCGTTAAACCAGTTAAGTAATACGGA
GTTTTACCAAGCGAACCTCGAAGAAGATTTATCTTCACAGCTTTGGGCAAAAGAGAAATTCGATAAAGTATTACTTGACC
CTGCACGTGCTGGAGCGAGTGGGATCGTTGATCAAATTTCAGCGTTAGGAGCGAAGCGTGTGGTTTATGTTTCGTGTAAT
CCTGCTACTCTAGCGAGAGATTCTGAGAGTTTAGAAAAACAAGGCTATCAATTAGCCAAACTTGGTATGTTGGATATGTT
TCCCCATACCAGTCATCTAGAATCGATGGCTCTCTTTATCAAGGCTTAG

Upstream 100 bases:

>100_bases
GTAAGCTTAATCAAAGCCGTGACGTTCGACAATGATTCCAAGACAGAATAGTGAGTCCTGGATACCAATTACTTATTAAA
AGATACATTAGGCACAAGCA

Downstream 100 bases:

>100_bases
AAGCCCTTAAATAAGGCTTAAAAATGAATCACAAAGGTGCAGGGAAGCCTGCGCTGATATCAATATGAGTCGAACTAACT
TGGAATCAGTTAGTCATATT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MARFFQPKKKTQLETKHQSVLVERMDHNGAGIAYQKNKPVFIDGALPGEKVVIQLTESKSKFSRAKLIKLLQPSELRLKP
FCKHFNQCGGCHLQHLGYSSQVEHKSQSLTHLMSKYQTANTEVALPIVGDESGYRRRARISLFVDKKTQLLQFGFRKKQS
KQIENITDCAVLDPRLNVLLPKLKSLLNTFSSLTSLGHVELVLGDTGPVMVLRHLKPLVEKDEQALMTLMTLAKEEGATL
YSMPETDKLVRLVGDAPSYSETGVTLPFEPNHFIQVNQKVNQQMVAQAIEWLEPQADERVLDLFCGLGNFSLPIAQQSAF
VVGVEGVDEMVQQATANAALNQLSNTEFYQANLEEDLSSQLWAKEKFDKVLLDPARAGASGIVDQISALGAKRVVYVSCN
PATLARDSESLEKQGYQLAKLGMLDMFPHTSHLESMALFIKA

Sequences:

>Translated_442_residues
MARFFQPKKKTQLETKHQSVLVERMDHNGAGIAYQKNKPVFIDGALPGEKVVIQLTESKSKFSRAKLIKLLQPSELRLKP
FCKHFNQCGGCHLQHLGYSSQVEHKSQSLTHLMSKYQTANTEVALPIVGDESGYRRRARISLFVDKKTQLLQFGFRKKQS
KQIENITDCAVLDPRLNVLLPKLKSLLNTFSSLTSLGHVELVLGDTGPVMVLRHLKPLVEKDEQALMTLMTLAKEEGATL
YSMPETDKLVRLVGDAPSYSETGVTLPFEPNHFIQVNQKVNQQMVAQAIEWLEPQADERVLDLFCGLGNFSLPIAQQSAF
VVGVEGVDEMVQQATANAALNQLSNTEFYQANLEEDLSSQLWAKEKFDKVLLDPARAGASGIVDQISALGAKRVVYVSCN
PATLARDSESLEKQGYQLAKLGMLDMFPHTSHLESMALFIKA
>Mature_441_residues
ARFFQPKKKTQLETKHQSVLVERMDHNGAGIAYQKNKPVFIDGALPGEKVVIQLTESKSKFSRAKLIKLLQPSELRLKPF
CKHFNQCGGCHLQHLGYSSQVEHKSQSLTHLMSKYQTANTEVALPIVGDESGYRRRARISLFVDKKTQLLQFGFRKKQSK
QIENITDCAVLDPRLNVLLPKLKSLLNTFSSLTSLGHVELVLGDTGPVMVLRHLKPLVEKDEQALMTLMTLAKEEGATLY
SMPETDKLVRLVGDAPSYSETGVTLPFEPNHFIQVNQKVNQQMVAQAIEWLEPQADERVLDLFCGLGNFSLPIAQQSAFV
VGVEGVDEMVQQATANAALNQLSNTEFYQANLEEDLSSQLWAKEKFDKVLLDPARAGASGIVDQISALGAKRVVYVSCNP
ATLARDSESLEKQGYQLAKLGMLDMFPHTSHLESMALFIKA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=189, Percent_Identity=33.8624338624339, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI51173878, Length=189, Percent_Identity=33.8624338624339, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI269784680, Length=187, Percent_Identity=28.8770053475936, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI269784676, Length=187, Percent_Identity=28.8770053475936, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI20149688, Length=187, Percent_Identity=28.8770053475936, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI269784678, Length=187, Percent_Identity=28.8770053475936, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1789148, Length=443, Percent_Identity=48.7584650112867, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1787083, Length=387, Percent_Identity=25.3229974160207, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790403, Length=181, Percent_Identity=28.7292817679558, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24666579, Length=416, Percent_Identity=23.7980769230769, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_VIBSL (B7VK72)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418183.1
- ProteinModelPortal:   B7VK72
- GeneID:   7162131
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2612
- HOGENOM:   HBG690163
- OMA:   IQVNQKV
- ProtClustDB:   PRK13168
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 49225; Mature: 49094

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 399-399 BINDING 275-275 BINDING 304-304 BINDING 309-309 BINDING 325-325 BINDING 352-352 BINDING 373-373

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFFQPKKKTQLETKHQSVLVERMDHNGAGIAYQKNKPVFIDGALPGEKVVIQLTESKS
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCEEEEEEECCHH
KFSRAKLIKLLQPSELRLKPFCKHFNQCGGCHLQHLGYSSQVEHKSQSLTHLMSKYQTAN
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
TEVALPIVGDESGYRRRARISLFVDKKTQLLQFGFRKKQSKQIENITDCAVLDPRLNVLL
CEEEEEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHH
PKLKSLLNTFSSLTSLGHVELVLGDTGPVMVLRHLKPLVEKDEQALMTLMTLAKEEGATL
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEE
YSMPETDKLVRLVGDAPSYSETGVTLPFEPNHFIQVNQKVNQQMVAQAIEWLEPQADERV
EECCCHHHHHHHHCCCCCCCCCCEECCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHH
LDLFCGLGNFSLPIAQQSAFVVGVEGVDEMVQQATANAALNQLSNTEFYQANLEEDLSSQ
HHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHH
LWAKEKFDKVLLDPARAGASGIVDQISALGAKRVVYVSCNPATLARDSESLEKQGYQLAK
HHHHHHHHHHHHCHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCHHHH
LGMLDMFPHTSHLESMALFIKA
HCHHHCCCCHHHHHHHHHHEEC
>Mature Secondary Structure 
ARFFQPKKKTQLETKHQSVLVERMDHNGAGIAYQKNKPVFIDGALPGEKVVIQLTESKS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCEEEEEEECCHH
KFSRAKLIKLLQPSELRLKPFCKHFNQCGGCHLQHLGYSSQVEHKSQSLTHLMSKYQTAN
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
TEVALPIVGDESGYRRRARISLFVDKKTQLLQFGFRKKQSKQIENITDCAVLDPRLNVLL
CEEEEEEECCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHH
PKLKSLLNTFSSLTSLGHVELVLGDTGPVMVLRHLKPLVEKDEQALMTLMTLAKEEGATL
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEE
YSMPETDKLVRLVGDAPSYSETGVTLPFEPNHFIQVNQKVNQQMVAQAIEWLEPQADERV
EECCCHHHHHHHHCCCCCCCCCCEECCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHH
LDLFCGLGNFSLPIAQQSAFVVGVEGVDEMVQQATANAALNQLSNTEFYQANLEEDLSSQ
HHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHH
LWAKEKFDKVLLDPARAGASGIVDQISALGAKRVVYVSCNPATLARDSESLEKQGYQLAK
HHHHHHHHHHHHCHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHCCCHHHH
LGMLDMFPHTSHLESMALFIKA
HCHHHCCCCHHHHHHHHHHEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA