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The map label for this gene is ispD

Identifier: 218710556

GI number: 218710556

Start: 2793239

End: 2793940

Strand: Reverse

Name: ispD

Synonym: VS_2605

Alternate gene names: 218710556

Gene position: 2793940-2793239 (Counterclockwise)

Preceding gene: 218710557

Following gene: 218710555

Centisome position: 84.68

GC content: 48.29

Gene sequence:

>702_bases
ATGTCGACTCAACTTCAAAGTGTGATTGCCGTTGTACCTGCAGCGGGCGTCGGTAGCCGAATGAAAGCAGACCGCCCTAA
GCAATATCTTAAGATTAACGGCAAAACGATTTTAGAGCACACCATTGAGAAATTACTGTCTCATCCACAAGTATCTCAAA
TTGTCGTTGCGATCAGCGATGATGACCCCTATTACCCGGAGTTAGCACTCAACCAGAATCCAAAAGTGGTCAGAGTGTCT
GGCGGAAGCGAACGAGCGGACTCGGTACTCTCTGCGCTAAACTGTATCGCTGAGCAGCAGCTTAGCGATTGGGTAATGGT
TCACGATGCGGCAAGGCCTTGTGTTCAACTCAGTGATATCGACAAGCTAATTTCCGGCGCGATGAGCCATGATGTAGGGG
CTATTTTAGCGGCGCCAGTTCGTGACACGATGAAGCGAGGTGCCCAAGGGCAGATCGAACATACCGTTGAGCGAGCCGAT
CTATGGCATGCACTTACGCCGCAAATGTTCAGAGCAAAGCCTCTGTGGAATGCATTAAGCGAGGCGTTGCAACAAGGCGT
TTCAATTACCGATGAAGCGTCAGCCTTTGAATGGAAAGGTTTATCGCCCGCTTTAGTGGCAGGGCGTTCAGATAATTTTA
AGATTACTCAGCCTGAAGATTTAGCACTTGCTGAGTTCTATTTAAGTCAGAATAAGGAATAA

Upstream 100 bases:

>100_bases
GCCAAGGCTTAGACGCGATAGAAGAGCGCGCACGTCATGAGCTTGGAATGGTAAAAGAAGGCGAAACGTTTTTCCGCATC
ATTGGTGAGGAATCCCATTA

Downstream 100 bases:

>100_bases
TGATGATTCGTATTGGCCATGGCTTTGATGTACATAAGTTTGGTGGTGAAGGTCCGGTAATTATAGGTGGCGTTAGAGTC
CCTTATGAGCAGGGTCTTAT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MSTQLQSVIAVVPAAGVGSRMKADRPKQYLKINGKTILEHTIEKLLSHPQVSQIVVAISDDDPYYPELALNQNPKVVRVS
GGSERADSVLSALNCIAEQQLSDWVMVHDAARPCVQLSDIDKLISGAMSHDVGAILAAPVRDTMKRGAQGQIEHTVERAD
LWHALTPQMFRAKPLWNALSEALQQGVSITDEASAFEWKGLSPALVAGRSDNFKITQPEDLALAEFYLSQNKE

Sequences:

>Translated_233_residues
MSTQLQSVIAVVPAAGVGSRMKADRPKQYLKINGKTILEHTIEKLLSHPQVSQIVVAISDDDPYYPELALNQNPKVVRVS
GGSERADSVLSALNCIAEQQLSDWVMVHDAARPCVQLSDIDKLISGAMSHDVGAILAAPVRDTMKRGAQGQIEHTVERAD
LWHALTPQMFRAKPLWNALSEALQQGVSITDEASAFEWKGLSPALVAGRSDNFKITQPEDLALAEFYLSQNKE
>Mature_232_residues
STQLQSVIAVVPAAGVGSRMKADRPKQYLKINGKTILEHTIEKLLSHPQVSQIVVAISDDDPYYPELALNQNPKVVRVSG
GSERADSVLSALNCIAEQQLSDWVMVHDAARPCVQLSDIDKLISGAMSHDVGAILAAPVRDTMKRGAQGQIEHTVERADL
WHALTPQMFRAKPLWNALSEALQQGVSITDEASAFEWKGLSPALVAGRSDNFKITQPEDLALAEFYLSQNKE

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=234, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789104, Length=233, Percent_Identity=52.7896995708155, Blast_Score=249, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_VIBSL (B7VK66)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002418177.1
- ProteinModelPortal:   B7VK66
- GeneID:   7162125
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2605
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- ProtClustDB:   PRK00155
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25451; Mature: 25320

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQLQSVIAVVPAAGVGSRMKADRPKQYLKINGKTILEHTIEKLLSHPQVSQIVVAISD
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHEEECCCHHHHHHHHHHHCCCCCCEEEEEECC
DDPYYPELALNQNPKVVRVSGGSERADSVLSALNCIAEQQLSDWVMVHDAARPCVQLSDI
CCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCHHHHHHH
DKLISGAMSHDVGAILAAPVRDTMKRGAQGQIEHTVERADLWHALTPQMFRAKPLWNALS
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHCCHHHHHHH
EALQQGVSITDEASAFEWKGLSPALVAGRSDNFKITQPEDLALAEFYLSQNKE
HHHHHCCCCCCCCCCCCCCCCCHHHEECCCCCEEEECCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STQLQSVIAVVPAAGVGSRMKADRPKQYLKINGKTILEHTIEKLLSHPQVSQIVVAISD
CHHHHHHHHHHCCCCCCCCCCCCCCHHHEEECCCHHHHHHHHHHHCCCCCCEEEEEECC
DDPYYPELALNQNPKVVRVSGGSERADSVLSALNCIAEQQLSDWVMVHDAARPCVQLSDI
CCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCHHHHHHH
DKLISGAMSHDVGAILAAPVRDTMKRGAQGQIEHTVERADLWHALTPQMFRAKPLWNALS
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHCCHHHHHHH
EALQQGVSITDEASAFEWKGLSPALVAGRSDNFKITQPEDLALAEFYLSQNKE
HHHHHCCCCCCCCCCCCCCCCCHHHEECCCCCEEEECCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA