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The map label for this gene is ispF [H]

Identifier: 218710555

GI number: 218710555

Start: 2792757

End: 2793239

Strand: Reverse

Name: ispF [H]

Synonym: VS_2604

Alternate gene names: 218710555

Gene position: 2793239-2792757 (Counterclockwise)

Preceding gene: 218710556

Following gene: 218710554

Centisome position: 84.66

GC content: 45.55

Gene sequence:

>483_bases
ATGATGATTCGTATTGGCCATGGCTTTGATGTACATAAGTTTGGTGGTGAAGGTCCGGTAATTATAGGTGGCGTTAGAGT
CCCTTATGAGCAGGGTCTTATCGCACACTCAGATGGTGATGTTGCCCTTCATGCGTTGAGTGACGCTTTATTAGGTGCGA
TTGCTGCCGGTGATATTGGTCGTCATTTCCCCGATACTGATGATGAGTGGAAAGGCGCGGATAGCCGTGAACTTCTAAAA
GATGTTTATCGTCGAGTAAAAGAACAAGGCTACGTGATTGGTAATGCCGATATTACTATCATGGCGCAAGCTCCAAAGAT
GGCGCCTCATATAGACTCTATGTGCCACGCTATCGCACAAGATTTAGAAACCAGCATTAGCAATGTGAATGTAAAAGCGA
CGACCACTGAGCGTTTAGGTTTTACAGGTCGCAAAGAGGGCATCGCATGTGAAGCGGTGGTTCTAATTACTAAAAGTGCG
TAA

Upstream 100 bases:

>100_bases
TATCGCCCGCTTTAGTGGCAGGGCGTTCAGATAATTTTAAGATTACTCAGCCTGAAGATTTAGCACTTGCTGAGTTCTAT
TTAAGTCAGAATAAGGAATA

Downstream 100 bases:

>100_bases
GCACCAACACGAAAAGAACAGCATGTCAGATATTTTATCTTCATTGGCTTATCTAAACGGTAAACCAACTGCGAAAGCAA
AACTAAAAGCAAAAGCCGAA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MMIRIGHGFDVHKFGGEGPVIIGGVRVPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGRHFPDTDDEWKGADSRELLK
DVYRRVKEQGYVIGNADITIMAQAPKMAPHIDSMCHAIAQDLETSISNVNVKATTTERLGFTGRKEGIACEAVVLITKSA

Sequences:

>Translated_160_residues
MMIRIGHGFDVHKFGGEGPVIIGGVRVPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGRHFPDTDDEWKGADSRELLK
DVYRRVKEQGYVIGNADITIMAQAPKMAPHIDSMCHAIAQDLETSISNVNVKATTTERLGFTGRKEGIACEAVVLITKSA
>Mature_160_residues
MMIRIGHGFDVHKFGGEGPVIIGGVRVPYEQGLIAHSDGDVALHALSDALLGAIAAGDIGRHFPDTDDEWKGADSRELLK
DVYRRVKEQGYVIGNADITIMAQAPKMAPHIDSMCHAIAQDLETSISNVNVKATTTERLGFTGRKEGIACEAVVLITKSA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=70.7006369426752, Blast_Score=211, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17122; Mature: 17122

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIRIGHGFDVHKFGGEGPVIIGGVRVPYEQGLIAHSDGDVALHALSDALLGAIAAGDIG
CEEEECCCCEEEEECCCCCEEEEEEECCHHCCEEEECCCCEEHHHHHHHHHHHHHHCCCC
RHFPDTDDEWKGADSRELLKDVYRRVKEQGYVIGNADITIMAQAPKMAPHIDSMCHAIAQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCHHHHHHHHHHHH
DLETSISNVNVKATTTERLGFTGRKEGIACEAVVLITKSA
HHHHHHHCCEEEEECHHHCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MMIRIGHGFDVHKFGGEGPVIIGGVRVPYEQGLIAHSDGDVALHALSDALLGAIAAGDIG
CEEEECCCCEEEEECCCCCEEEEEEECCHHCCEEEECCCCEEHHHHHHHHHHHHHHCCCC
RHFPDTDDEWKGADSRELLKDVYRRVKEQGYVIGNADITIMAQAPKMAPHIDSMCHAIAQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCHHHHHHHHHHHH
DLETSISNVNVKATTTERLGFTGRKEGIACEAVVLITKSA
HHHHHHHCCEEEEECHHHCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]