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The map label for this gene is rpoS [H]

Identifier: 218710550

GI number: 218710550

Start: 2788212

End: 2789219

Strand: Reverse

Name: rpoS [H]

Synonym: VS_2599

Alternate gene names: 218710550

Gene position: 2789219-2788212 (Counterclockwise)

Preceding gene: 218710551

Following gene: 218710548

Centisome position: 84.54

GC content: 45.04

Gene sequence:

>1008_bases
ATGCTAATTTCGCCAGGGGGAGGCGTTATGAGTATAAGCAATGCAGTAACCAAAGAAGAGTTCGATCTTAGCCAAGCAAC
CACGGAACAGGAAGCTCTTGGAAAAGCAAAACGAACAGTCACTAAGAAAACCGAAGCGAAAGAAGAGACTGAAGTTACGT
CTAAAAGCTTAGATGCGACTCAACTCTACTTAGGCGAAATCGGTTTCTCACCACTATTAACCGCTGAAGAAGAAGTGCTT
TATGCACGTCGAGCTCTACGCGGTGATGAAGCAGCACGCAAACGCATGATCGAAAGTAACCTGCGTTTGGTGGTAAAAAT
TTCTCGTCGTTATAGCAACCGTGGTCTTGCACTTCTCGACCTTATTGAAGAAGGCAACCTAGGTTTGATTCGCGCCGTAG
AGAAGTTTGACCCAGAGCGTGGCTTCCGTTTCTCAACTTACGCAACATGGTGGATTCGTCAAACCATTGAACGTGCGCTA
ATGAATCAGACTCGCACTATCCGTTTGCCAATCCATGTTGTGAAAGAGCTGAACATCTACCTACGCACCGCAAGAGAACT
TTCACAAAAACTTGACCATGAACCAACGGCAGAAGAAATTGCTTCTAAGCTAGACAAACCTGTTGGTGATGTGAGCAAGA
TGCTTCGTCTAAACGAAAGAGTGAGCTCTGTTGATACGCCTATTGGTGGTGATGGTGAGAAAGCGCTACTGGATATAATT
CCGGACATCAACAACTCAGACCCTGAGGTTTCAACTCAAGATAGCGATATTAAGAACTCGTTGATTTTCTGGCTTGATGA
GCTGAATCCTAAGCAGAAAGAGGTGCTTGCGCGTCGCTTTGGATTACTTGGCTATGAACCATCAACACTAGAAGAAGTAG
GCCGTGAAATTAGCCTAACTCGTGAACGCGTTCGCCAAATCCAAGTTGAAGGTCTGCGTCGTCTACGTGAGATTTTAATC
AAGCAAGGCTTGAACATGGAAAATCTGTTCAACGTAGAAAACGACTAA

Upstream 100 bases:

>100_bases
CAGTTAATCCAAAACGGTATTTACCTTAAATACAATATGTAAAAATTACGTATTGTATGGAGTGACATAGTCATTTAGCG
ACATATGAAGTTGTAATGTC

Downstream 100 bases:

>100_bases
ATGAACTGATGATGTTTCTTTGAATAGAAGACAAATAAATACGCTGACTTTAAGTCAGCGTTTTTTTGTTTGTGGCTTTT
GTTGTTCATGCTGCCGACTC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: Sigma-38 factor [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MLISPGGGVMSISNAVTKEEFDLSQATTEQEALGKAKRTVTKKTEAKEETEVTSKSLDATQLYLGEIGFSPLLTAEEEVL
YARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAL
MNQTRTIRLPIHVVKELNIYLRTARELSQKLDHEPTAEEIASKLDKPVGDVSKMLRLNERVSSVDTPIGGDGEKALLDII
PDINNSDPEVSTQDSDIKNSLIFWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREISLTRERVRQIQVEGLRRLREILI
KQGLNMENLFNVEND

Sequences:

>Translated_335_residues
MLISPGGGVMSISNAVTKEEFDLSQATTEQEALGKAKRTVTKKTEAKEETEVTSKSLDATQLYLGEIGFSPLLTAEEEVL
YARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAL
MNQTRTIRLPIHVVKELNIYLRTARELSQKLDHEPTAEEIASKLDKPVGDVSKMLRLNERVSSVDTPIGGDGEKALLDII
PDINNSDPEVSTQDSDIKNSLIFWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREISLTRERVRQIQVEGLRRLREILI
KQGLNMENLFNVEND
>Mature_335_residues
MLISPGGGVMSISNAVTKEEFDLSQATTEQEALGKAKRTVTKKTEAKEETEVTSKSLDATQLYLGEIGFSPLLTAEEEVL
YARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAL
MNQTRTIRLPIHVVKELNIYLRTARELSQKLDHEPTAEEIASKLDKPVGDVSKMLRLNERVSSVDTPIGGDGEKALLDII
PDINNSDPEVSTQDSDIKNSLIFWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREISLTRERVRQIQVEGLRRLREILI
KQGLNMENLFNVEND

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. May be required for the persistence of V.cholerae in aquatic habitats [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=279, Percent_Identity=81.7204301075269, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=43.8053097345133, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=26.8551236749117, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38036; Mature: 38036

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISPGGGVMSISNAVTKEEFDLSQATTEQEALGKAKRTVTKKTEAKEETEVTSKSLDAT
CEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLYLGEIGFSPLLTAEEEVLYARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLD
HHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEHHHCCCCEEEEH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALMNQTRTIRLPIHVVKELNIY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
LRTARELSQKLDHEPTAEEIASKLDKPVGDVSKMLRLNERVSSVDTPIGGDGEKALLDII
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
PDINNSDPEVSTQDSDIKNSLIFWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREISLT
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RERVRQIQVEGLRRLREILIKQGLNMENLFNVEND
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MLISPGGGVMSISNAVTKEEFDLSQATTEQEALGKAKRTVTKKTEAKEETEVTSKSLDAT
CEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLYLGEIGFSPLLTAEEEVLYARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLD
HHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEHHHCCCCEEEEH
LIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALMNQTRTIRLPIHVVKELNIY
HHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
LRTARELSQKLDHEPTAEEIASKLDKPVGDVSKMLRLNERVSSVDTPIGGDGEKALLDII
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
PDINNSDPEVSTQDSDIKNSLIFWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREISLT
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RERVRQIQVEGLRRLREILIKQGLNMENLFNVEND
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473029; 10952301 [H]