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The map label for this gene is alaS [H]

Identifier: 218710534

GI number: 218710534

Start: 2766512

End: 2769094

Strand: Reverse

Name: alaS [H]

Synonym: VS_2583

Alternate gene names: 218710534

Gene position: 2769094-2766512 (Counterclockwise)

Preceding gene: 218710535

Following gene: 218710533

Centisome position: 83.93

GC content: 47.27

Gene sequence:

>2583_bases
ATGTACATGAGCACTGATGAGGTTCGCAACGCGTTCCTTAAGTTCTTTGAGAGCAAAGAACACCAAATCGTAGACAGTTC
ATCGTTAGTTCCACATAACGATCCAACCCTGCTATTTACTAACGCAGGCATGAACCAATTCAAAGATTGCTTCTTAGGCG
CCGAAAAACGTGCCTACACTCGAGCAACTACGGCTCAGCGTTGTGTACGTGCGGGTGGTAAACACAATGACCTTGAAAAC
GTAGGTTTCACGGCTCGTCACCACACGTTCTTCGAAATGCTAGGTAACTTCAGCTTTGGCGACTACTTCAAAGAAGAAGC
GATTGGCTTTGCTTGGGAATTCCTGACTGAAACACTAGGTCTGCCAAAAGATCGCCTGTTAGTCACGGTTTACGAGACAG
ATGACGAAGCATTCGAAATCTGGAACAAGAAAGTGGGCATCCCAGCTGACAAGATTGTTCGCATCGGTGACAAAGAAGGT
GGCAAGAAGTTTGAATCTGATAACTTCTGGACAATGGGTGACACAGGTCCTTGTGGTCCATGTACTGAGATCTTCTACGA
TCACGGTGAACACATTTGGGGCGGTCGTCCTGGTACACCTGAAGAAGATGGTGACCGTTTCATCGAGATTTGGAACAACG
TATTCATGCAGTTCAACCGTCACGCTGACGGCACTATGGAACCGCTACCTAAGCCAGCTGTAGATACTGGTATGGGTATT
GAGCGTATCTCTGCAATCATGCAGGGTGTTCACTCTAACTACGAAATTGATGTATTCCAAAAGCTAATCAAAGCGACAGC
AGAAGTAGTGGGTCACGACGACCTATCGAACCAATCACTGCGCGTTATTGCTGACCACATCCGTTCTTGTTCATTCCTAA
TCGCTGATGGCGTAATGCCTTCAAACGAAGGTCGTGGCTACGTTCTACGTCGTATTATTCGTCGTGCAGTTCGTCACGGT
AACAAGATTGGTGCACAAGGCGTGTTCTTCCACAAACTTGTGGGTGTTCTGGCTGAAGTGATGGGTTCTGCGGCTGAAGA
GCTTAAGAAACAGCAAGAGCTTGTTGAAAAAGTACTTCGCATCGAAGAAGAGAACTTTGGTCGCACGCTAGAGCGCGGCA
TGGTTATCCTTAACGACGCATTAGACGCACTTGAAGGCAAAGAGCTTGACGGTGAAACAGTCTTCAAACTTTACGATACA
TACGGCTTCCCAGCTGACCTTACTAACGATGTAGCTCGTGAACGCGACTTTACTATCGATGAAGCAGGCTTTGAGAAGGC
GATGGAAGCACAGCGTAAGCGTGCACGTGAAGCGGGTCAGTTCGGTACTGATTACAACGCAACGATCAAAGTAGACACGA
ACACTGAGTTCTGTGGTTACACAGGTACTGAAGGTGCGGGTGAAATTTCTGCGTTATTCGTTGACGGCGAAGAAGTATCT
TCACTATCGGCTGGCGACAAAGCAATCATCATCCTTGAAGAGACACCATTCTACGCTGAGTCAGGCGGTCAATGTGGTGA
CGCGGGTACGCTAAGCACAGCGTCAGGTCTATTCAAAGTACAAGATACTCAAAAGCTAGGTAATGCATTTTCACACCACG
GTGAGCTAGTTGAAGGCGTATTTGCTCAAGGCGATAAAGTAGAAGCGCGCGTTGATGCTGAGCGTCGTGCTGCTATCTCA
CTAAACCACTCTGCAACTCACTTACTTCACTCTGCATTGCGCGAAGTGCTAGGTGAGCACGTTGCTCAGAAAGGTTCTTT
AGTTAAGCCAGACAACCTACGTTTTGACTTCTCTAACCTTGAAGCGGTAACACCAGCACAGATTAAAGAAGTAGAGCGTC
TAGTTAACGCACAAGTTCGTAAGAACCATGTGATTGAAACTAACATCATGGACATCGAGTCAGCGAAAGAAAAAGGTGCG
ATGGCACTGTTTGGCGAGAAGTACGATGATGAAGTTCGCGTTCTGTCGATGGGCGATTTCTCTACTGAGCTTTGTGGTGG
TATCCACGCAAGCAACACGGGTGATATCGGTCTATTCAAGATCACTTCTGAAGGTGGTATTGCTGCGGGTGTTCGTCGTA
TTGAAGCGGTAACGGGTGAAGCAGCTCTAGATGCAGTAGAAGCTCAGGCTAACGCTTACGAAGAGAAGCTTGCTGAATCA
GCTAAGAAAGCGAAATCGCTAGAGAAAGAAATCCAACTACTTAAAGAAAAGATGGCTGCAGCTGAAGGTGCAAACATCAT
GGGTAAAGTAGAAGAGATCTCTGGTACTAAGGTACTGGTTGCTGCCATTGAAGGCGCAGACAACAAGAACCTACGTACTA
TGGTTGATAATGCTAAAAACCAAGTGGGTAGCGGCATTATCCTGATCGCGAACGTAGCAGACGGTAAAGTTGGCTTAATT
GCTGGCGTAACCAAAGACCTTATCGGCAAAGTTAAAGCGGGTGACCTCGTTAAGATGGTTGCTGAGCAAGTTGGCGGTAA
AGGCGGCGGTCGTCCAGATATGGCTCAAGCGGGTGGTACTGACGTTGAAGCGCTACCTGAAGCGCTTAAATCAGTACGTA
TTTGGCTTGAAGAGCGTCTTTAA

Upstream 100 bases:

>100_bases
CTGCCCAAGGGTGTCTTTTCTACAAGAAAACTAGTCGAAGCCTTAACCATGATCTACAATACGGCAAAATTCTAACTCGA
CTATTTTCAGGAAGAGCTGC

Downstream 100 bases:

>100_bases
GCTTAAGTAATTGATACAAAGATGCCCAATCAACTGATTGGGCATCTTTTATTTTGCCCATTGGAAGAACCTTTGAAAAA
GGTTTGATTGAGATTAACCA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MYMSTDEVRNAFLKFFESKEHQIVDSSSLVPHNDPTLLFTNAGMNQFKDCFLGAEKRAYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKEEAIGFAWEFLTETLGLPKDRLLVTVYETDDEAFEIWNKKVGIPADKIVRIGDKEG
GKKFESDNFWTMGDTGPCGPCTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPAVDTGMGI
ERISAIMQGVHSNYEIDVFQKLIKATAEVVGHDDLSNQSLRVIADHIRSCSFLIADGVMPSNEGRGYVLRRIIRRAVRHG
NKIGAQGVFFHKLVGVLAEVMGSAAEELKKQQELVEKVLRIEEENFGRTLERGMVILNDALDALEGKELDGETVFKLYDT
YGFPADLTNDVARERDFTIDEAGFEKAMEAQRKRAREAGQFGTDYNATIKVDTNTEFCGYTGTEGAGEISALFVDGEEVS
SLSAGDKAIIILEETPFYAESGGQCGDAGTLSTASGLFKVQDTQKLGNAFSHHGELVEGVFAQGDKVEARVDAERRAAIS
LNHSATHLLHSALREVLGEHVAQKGSLVKPDNLRFDFSNLEAVTPAQIKEVERLVNAQVRKNHVIETNIMDIESAKEKGA
MALFGEKYDDEVRVLSMGDFSTELCGGIHASNTGDIGLFKITSEGGIAAGVRRIEAVTGEAALDAVEAQANAYEEKLAES
AKKAKSLEKEIQLLKEKMAAAEGANIMGKVEEISGTKVLVAAIEGADNKNLRTMVDNAKNQVGSGIILIANVADGKVGLI
AGVTKDLIGKVKAGDLVKMVAEQVGGKGGGRPDMAQAGGTDVEALPEALKSVRIWLEERL

Sequences:

>Translated_860_residues
MYMSTDEVRNAFLKFFESKEHQIVDSSSLVPHNDPTLLFTNAGMNQFKDCFLGAEKRAYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKEEAIGFAWEFLTETLGLPKDRLLVTVYETDDEAFEIWNKKVGIPADKIVRIGDKEG
GKKFESDNFWTMGDTGPCGPCTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPAVDTGMGI
ERISAIMQGVHSNYEIDVFQKLIKATAEVVGHDDLSNQSLRVIADHIRSCSFLIADGVMPSNEGRGYVLRRIIRRAVRHG
NKIGAQGVFFHKLVGVLAEVMGSAAEELKKQQELVEKVLRIEEENFGRTLERGMVILNDALDALEGKELDGETVFKLYDT
YGFPADLTNDVARERDFTIDEAGFEKAMEAQRKRAREAGQFGTDYNATIKVDTNTEFCGYTGTEGAGEISALFVDGEEVS
SLSAGDKAIIILEETPFYAESGGQCGDAGTLSTASGLFKVQDTQKLGNAFSHHGELVEGVFAQGDKVEARVDAERRAAIS
LNHSATHLLHSALREVLGEHVAQKGSLVKPDNLRFDFSNLEAVTPAQIKEVERLVNAQVRKNHVIETNIMDIESAKEKGA
MALFGEKYDDEVRVLSMGDFSTELCGGIHASNTGDIGLFKITSEGGIAAGVRRIEAVTGEAALDAVEAQANAYEEKLAES
AKKAKSLEKEIQLLKEKMAAAEGANIMGKVEEISGTKVLVAAIEGADNKNLRTMVDNAKNQVGSGIILIANVADGKVGLI
AGVTKDLIGKVKAGDLVKMVAEQVGGKGGGRPDMAQAGGTDVEALPEALKSVRIWLEERL
>Mature_860_residues
MYMSTDEVRNAFLKFFESKEHQIVDSSSLVPHNDPTLLFTNAGMNQFKDCFLGAEKRAYTRATTAQRCVRAGGKHNDLEN
VGFTARHHTFFEMLGNFSFGDYFKEEAIGFAWEFLTETLGLPKDRLLVTVYETDDEAFEIWNKKVGIPADKIVRIGDKEG
GKKFESDNFWTMGDTGPCGPCTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPAVDTGMGI
ERISAIMQGVHSNYEIDVFQKLIKATAEVVGHDDLSNQSLRVIADHIRSCSFLIADGVMPSNEGRGYVLRRIIRRAVRHG
NKIGAQGVFFHKLVGVLAEVMGSAAEELKKQQELVEKVLRIEEENFGRTLERGMVILNDALDALEGKELDGETVFKLYDT
YGFPADLTNDVARERDFTIDEAGFEKAMEAQRKRAREAGQFGTDYNATIKVDTNTEFCGYTGTEGAGEISALFVDGEEVS
SLSAGDKAIIILEETPFYAESGGQCGDAGTLSTASGLFKVQDTQKLGNAFSHHGELVEGVFAQGDKVEARVDAERRAAIS
LNHSATHLLHSALREVLGEHVAQKGSLVKPDNLRFDFSNLEAVTPAQIKEVERLVNAQVRKNHVIETNIMDIESAKEKGA
MALFGEKYDDEVRVLSMGDFSTELCGGIHASNTGDIGLFKITSEGGIAAGVRRIEAVTGEAALDAVEAQANAYEEKLAES
AKKAKSLEKEIQLLKEKMAAAEGANIMGKVEEISGTKVLVAAIEGADNKNLRTMVDNAKNQVGSGIILIANVADGKVGLI
AGVTKDLIGKVKAGDLVKMVAEQVGGKGGGRPDMAQAGGTDVEALPEALKSVRIWLEERL

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=980, Percent_Identity=36.9387755102041, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI38569417, Length=951, Percent_Identity=33.9642481598318, Blast_Score=449, Evalue=1e-126,
Organism=Escherichia coli, GI1789048, Length=876, Percent_Identity=66.4383561643836, Blast_Score=1195, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=978, Percent_Identity=37.9345603271984, Blast_Score=560, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17536681, Length=796, Percent_Identity=33.4170854271357, Blast_Score=399, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324911, Length=766, Percent_Identity=44.1253263707572, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24582809, Length=977, Percent_Identity=37.2569089048106, Blast_Score=563, Evalue=1e-160,
Organism=Drosophila melanogaster, GI45552267, Length=977, Percent_Identity=37.2569089048106, Blast_Score=563, Evalue=1e-160,
Organism=Drosophila melanogaster, GI24658214, Length=847, Percent_Identity=29.7520661157025, Blast_Score=342, Evalue=8e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 93929; Mature: 93929

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMSTDEVRNAFLKFFESKEHQIVDSSSLVPHNDPTLLFTNAGMNQFKDCFLGAEKRAYT
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHH
RATTAQRCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFKEEAIGFAWEFLTETLG
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
LPKDRLLVTVYETDDEAFEIWNKKVGIPADKIVRIGDKEGGKKFESDNFWTMGDTGPCGP
CCCCCEEEEEEECCCHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCCCCEECCCCCCCCH
CTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPAVDTGMGI
HHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
ERISAIMQGVHSNYEIDVFQKLIKATAEVVGHDDLSNQSLRVIADHIRSCSFLIADGVMP
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEECCCCC
SNEGRGYVLRRIIRRAVRHGNKIGAQGVFFHKLVGVLAEVMGSAAEELKKQQELVEKVLR
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEEENFGRTLERGMVILNDALDALEGKELDGETVFKLYDTYGFPADLTNDVARERDFTID
HHHHHHHHHHHHCEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
EAGFEKAMEAQRKRAREAGQFGTDYNATIKVDTNTEFCGYTGTEGAGEISALFVDGEEVS
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCHHHH
SLSAGDKAIIILEETPFYAESGGQCGDAGTLSTASGLFKVQDTQKLGNAFSHHGELVEGV
HCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHH
FAQGDKVEARVDAERRAAISLNHSATHLLHSALREVLGEHVAQKGSLVKPDNLRFDFSNL
HCCCCCEEEHCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
EAVTPAQIKEVERLVNAQVRKNHVIETNIMDIESAKEKGAMALFGEKYDDEVRVLSMGDF
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCEEEECCCCCCCEEEEEECCC
STELCGGIHASNTGDIGLFKITSEGGIAAGVRRIEAVTGEAALDAVEAQANAYEEKLAES
HHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
AKKAKSLEKEIQLLKEKMAAAEGANIMGKVEEISGTKVLVAAIEGADNKNLRTMVDNAKN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
QVGSGIILIANVADGKVGLIAGVTKDLIGKVKAGDLVKMVAEQVGGKGGGRPDMAQAGGT
HCCCCEEEEEEECCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCC
DVEALPEALKSVRIWLEERL
CHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYMSTDEVRNAFLKFFESKEHQIVDSSSLVPHNDPTLLFTNAGMNQFKDCFLGAEKRAYT
CCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHH
RATTAQRCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFKEEAIGFAWEFLTETLG
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
LPKDRLLVTVYETDDEAFEIWNKKVGIPADKIVRIGDKEGGKKFESDNFWTMGDTGPCGP
CCCCCEEEEEEECCCHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCCCCEECCCCCCCCH
CTEIFYDHGEHIWGGRPGTPEEDGDRFIEIWNNVFMQFNRHADGTMEPLPKPAVDTGMGI
HHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
ERISAIMQGVHSNYEIDVFQKLIKATAEVVGHDDLSNQSLRVIADHIRSCSFLIADGVMP
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEECCCCC
SNEGRGYVLRRIIRRAVRHGNKIGAQGVFFHKLVGVLAEVMGSAAEELKKQQELVEKVLR
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEEENFGRTLERGMVILNDALDALEGKELDGETVFKLYDTYGFPADLTNDVARERDFTID
HHHHHHHHHHHHCEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
EAGFEKAMEAQRKRAREAGQFGTDYNATIKVDTNTEFCGYTGTEGAGEISALFVDGEEVS
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCHHHH
SLSAGDKAIIILEETPFYAESGGQCGDAGTLSTASGLFKVQDTQKLGNAFSHHGELVEGV
HCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHH
FAQGDKVEARVDAERRAAISLNHSATHLLHSALREVLGEHVAQKGSLVKPDNLRFDFSNL
HCCCCCEEEHCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
EAVTPAQIKEVERLVNAQVRKNHVIETNIMDIESAKEKGAMALFGEKYDDEVRVLSMGDF
CCCCHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCCEEEECCCCCCCEEEEEECCC
STELCGGIHASNTGDIGLFKITSEGGIAAGVRRIEAVTGEAALDAVEAQANAYEEKLAES
HHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
AKKAKSLEKEIQLLKEKMAAAEGANIMGKVEEISGTKVLVAAIEGADNKNLRTMVDNAKN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
QVGSGIILIANVADGKVGLIAGVTKDLIGKVKAGDLVKMVAEQVGGKGGGRPDMAQAGGT
HCCCCEEEEEEECCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCC
DVEALPEALKSVRIWLEERL
CHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA