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The map label for this gene is pilD [H]

Identifier: 218710511

GI number: 218710511

Start: 2742960

End: 2743829

Strand: Direct

Name: pilD [H]

Synonym: VS_2550

Alternate gene names: 218710511

Gene position: 2742960-2743829 (Clockwise)

Preceding gene: 218710510

Following gene: 218710512

Centisome position: 83.14

GC content: 44.6

Gene sequence:

>870_bases
ATGGAAGTATTTCACTACTATCCTTGGCTATTCCCCGTATTAGCTTTCATTTTTAGCCTTCTTATTGGCAGTTTCCTCAA
CGTAGTCATACACCGTTTACCGATTATGATGGAACGAGAGTGGCAACAAGAGTGCTCGGAGTATTTCTCTCAATATAAAA
TTCCAGCGCCAGAGGGGAAGTTCAATCTCAGTATCCCTCGTTCGGCTTGCCCGAAATGTAAAACCCAATTAAGAATCGTC
GACAATGTTCCAGTATTAAGCTGGTTGTTCTTGAAAGGTAAGTGTCATAGCTGTGCTAACCCAATCAGTGCTCGCTATCC
ATTGGTCGAACTGCTGACTGCAATACTATGCACCGTAGTGGCTAGCCACTTTGGTTTCAATTACTACGCCATTGCTTTGA
TTTTTTTCACCTTTGCATTGATTGCCGCAACCTTTATCGACCTAGATACCATGCTGTTACCTGATCAAATCACCTTACCT
TTGGTTTGGTCTGGTATCGCGTTAGCACTGTTTAACATCAGCCCAGTATCGCTTCAAGATTCCGTAGTTGGCGCAATGGC
CGGCTACCTAGCTTTGTGGTCTGTTTATTGGTTGTTCAAGCTACTAACAGGCAAAGAAGGCATGGGATATGGAGACTTCA
AACTCCTAGCCGCATTAGGTGCATGGCTTGGCTGGCAACACCTACCAATGATCATTCTTTTGTCGTCGCTGGTCGGTTTG
GTTTTCGGCTTGATTCAACTTCGCTTGAAACAGCAAGGCATAGATAAAGCGTTCCCATTTGGACCTTACCTGGCGATTGC
GGGTTGGGTAAGTTTGATGTGGGGCAATGACATCATGGGTTGGTATTTCACTTCTGTACTAGGGATTTAA

Upstream 100 bases:

>100_bases
CGATCTTTAATCTTATGAGTGTGTTAGGATAGCTAAAAGCAACCAACCCCCCCTCAAGCAGAGAACCACTGCTTGTATTG
ATTAAGTGGTAAAGGACACT

Downstream 100 bases:

>100_bases
TCATGGCAATCATTATCGGATTAAGCGGTGGGATCGCCAGTGGTAAAACCACAGTAGCCAACCTGTTCAATGAGCATTTC
AATATTGATATTGTCGATGC

Product: type IV prepilin-like proteins leader peptide processing enzyme

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGKFNLSIPRSACPKCKTQLRIV
DNVPVLSWLFLKGKCHSCANPISARYPLVELLTAILCTVVASHFGFNYYAIALIFFTFALIAATFIDLDTMLLPDQITLP
LVWSGIALALFNISPVSLQDSVVGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMGWYFTSVLGI

Sequences:

>Translated_289_residues
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGKFNLSIPRSACPKCKTQLRIV
DNVPVLSWLFLKGKCHSCANPISARYPLVELLTAILCTVVASHFGFNYYAIALIFFTFALIAATFIDLDTMLLPDQITLP
LVWSGIALALFNISPVSLQDSVVGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMGWYFTSVLGI
>Mature_289_residues
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGKFNLSIPRSACPKCKTQLRIV
DNVPVLSWLFLKGKCHSCANPISARYPLVELLTAILCTVVASHFGFNYYAIALIFFTFALIAATFIDLDTMLLPDQITLP
LVWSGIALALFNISPVSLQDSVVGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMGWYFTSVLGI

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=271, Percent_Identity=40.590405904059, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1789732, Length=141, Percent_Identity=46.0992907801418, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32511; Mature: 32511

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9826339 [H]