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The map label for this gene is pnp [H]

Identifier: 218710441

GI number: 218710441

Start: 2649082

End: 2651202

Strand: Reverse

Name: pnp [H]

Synonym: VS_2478

Alternate gene names: 218710441

Gene position: 2651202-2649082 (Counterclockwise)

Preceding gene: 218710442

Following gene: 218710440

Centisome position: 80.36

GC content: 45.4

Gene sequence:

>2121_bases
ATGTTTGAAAAACCAGTTGTAAAGTCATTCCAGTACGGTAACCACACCGTTACTCTAGAAACGGGCGTAATGGCACGTCA
AGCTACTGCTGCTGTAATGGCGACTATGGACGATACATCAGTATTCGTTTCTGTTGTTGCTAAAAAAGAAGCTGTTGCGG
GTCAAGATTTCTTCCCTCTAACAGTTAACTACCAAGAGCGTACATACGCTGCGGGTAAAATCCCTGGTGGTTTCTTCAAG
CGCGAAGGTCGTCCATCTGAAGGCGAAACACTAACAGCTCGTCTGATTGACCGTCCAATTCGTCCACTTTTCCCAAGTGC
GTTTAAAAACGAAGTTCAAGTTATCGCTACGGTTGTTTCTATCAACCCTGACGTAAACCCAGACATGATCACTATGATCG
CAACGTCTGCTGCACTTGCTATCTCTGGTGCTCCATTCAATGGTCCTATCGGTGCTGCACGTGTTGGTCACATCGACGGC
GAACTTGTTCTTAACCCATCAAATACTGAGCTTGAAAACTCTAAACTAGACCTAGTTGTGTCTGGTACAGAAGGCGCAGT
ACTTATGGTTGAATCTGAAGCAGATAACCTATCTGAAGAAGAAATGCTTTCAGCTGTTGTTTACGGTCACGACCAACAAC
AAGTTGTAATCAAAGCAATCAACGAGTTTGCTGCTGAAGTTGCAACTCCATCTTGGAACTGGGAAGCGCCAGCAGTTAAT
ACTGAGCTTAAAGCTCAAGTTGCTGAACTTGCTGAAACTCGTCTATCTGACGCGTACCAGATCACTGAGAAAATGGCTCG
TTACGAGCAAGTTGGCGCAATCAAGAACGACACTGTTGAAGCTCTAATTGCACAAGACGAAAACCTAGATGAGCGCGAAA
TCCGCGGCATGCTTGGTTCTCTAGAGAAAAACGTAGTACGTAGCCGCATCATTGCTGGCAACCCACGTATCGATGGCCGT
GAAAAAGACATGGTTCGTGCGCTAGACGTACGTACTGGTGTTCTTCCACGTACACACGGTTCTTCTCTATTCACTCGTGG
TGAAACTCAAGCACTTGTTACTGCAACGCTTGGTACACAACGTGATGCACAAATCATCGACAGCCTAATGGGTGAGAAGA
AAGACAACTTCCTTCTACACTACAACTTCCCTCCATACTGTGTAGGTGAGACTGGTTTCGTTGGTTCTCCTAAGCGTCGT
GAAATTGGTCACGGTAAGCTTGCTAAACGTGGTATCCAAGCAGTAATGCCTTCTGTTGATGAATTCCCATACACAGTTCG
TGTTGTATCGGAAATCACTGAATCTAACGGTTCTTCTTCAATGGCTTCTGTATGTGGTACATCTCTAGCTCTTATGGATG
CTGGTGTTCCAATCAAAGCTTCTGTTGCGGGTATCGCAATGGGTCTTGTTAAAGAAGGCGACGATTTCGTTGTTCTTTCT
GACATCCTTGGCGACGAAGATCACCTAGGTGATATGGACTTTAAAGTAGCAGGTACTAACGCTGGTATCACTGCACTTCA
AATGGACATCAAGATCGAAGGTATCACTAAAGAGATCATGCAAATTGCTCTTAACCAAGCGCAAGGTGCACGTAAGCACA
TCCTTTCTGTAATGGATGAAGCTATCTCTGGTGCTCGTGAAGATATCTCTGAATTCGCTCCACGTATCCACACAATGAAA
ATCAGCTCTGATAAGATCAAAGATGTTATCGGTAAAGGCGGCGCAGTTATCCGTGCTCTTTGTGAAGAAACGGGTACTAC
AATCGAAATCGAAGACGATGGCACAATCAAGATTGCTGCTACTGAAGGCGCAGCTGCTAAAGAAGCTATCCGTCGTATCG
AAGAGATCACTGCTGAAGTTGAAGTTGGCCGCATTTACCAAGGTAAAGTTGCTCGTCTAGCTGACTTCGGTGCATTCGTT
ACTATCCTTCCAGGTAAAGATGGTCTAGTACACATCTCTCAAATCGCTGATAAGCGCGTTGAGAAAGTGTCTGACTACCT
AACTGAAGGTCAAGAAGTACCAGTTAAGGTTCTTGAAATTGACCGTCAAGGCCGTGTACGTCTAAGCATGAAAGAAGCAG
TTGAAACGCCTGCTGAAGGCGAAGCACCTGCTGCTGAGTAA

Upstream 100 bases:

>100_bases
TCTGATTGAACTTCTCGTCTTTTTTTACTAGTCGCGATTAGTTATTGCTGAGCATTCATTCACTTATTCTGAAAAGAGAT
TTTCAGATGAAGGATATACA

Downstream 100 bases:

>100_bases
TTCTTAGCCTATTAATGGGACTATCGCTTTTTAGTGATTTCTAACCCATTGGTAAAAAAGCATGTTATAAAGGGGAGCAT
ATCGCTCCCCTTTTTTATTG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MFEKPVVKSFQYGNHTVTLETGVMARQATAAVMATMDDTSVFVSVVAKKEAVAGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVSINPDVNPDMITMIATSAALAISGAPFNGPIGAARVGHIDG
ELVLNPSNTELENSKLDLVVSGTEGAVLMVESEADNLSEEEMLSAVVYGHDQQQVVIKAINEFAAEVATPSWNWEAPAVN
TELKAQVAELAETRLSDAYQITEKMARYEQVGAIKNDTVEALIAQDENLDEREIRGMLGSLEKNVVRSRIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQRDAQIIDSLMGEKKDNFLLHYNFPPYCVGETGFVGSPKRR
EIGHGKLAKRGIQAVMPSVDEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
DILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILSVMDEAISGAREDISEFAPRIHTMK
ISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGRIYQGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVPVKVLEIDRQGRVRLSMKEAVETPAEGEAPAAE

Sequences:

>Translated_706_residues
MFEKPVVKSFQYGNHTVTLETGVMARQATAAVMATMDDTSVFVSVVAKKEAVAGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVSINPDVNPDMITMIATSAALAISGAPFNGPIGAARVGHIDG
ELVLNPSNTELENSKLDLVVSGTEGAVLMVESEADNLSEEEMLSAVVYGHDQQQVVIKAINEFAAEVATPSWNWEAPAVN
TELKAQVAELAETRLSDAYQITEKMARYEQVGAIKNDTVEALIAQDENLDEREIRGMLGSLEKNVVRSRIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQRDAQIIDSLMGEKKDNFLLHYNFPPYCVGETGFVGSPKRR
EIGHGKLAKRGIQAVMPSVDEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
DILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILSVMDEAISGAREDISEFAPRIHTMK
ISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGRIYQGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVPVKVLEIDRQGRVRLSMKEAVETPAEGEAPAAE
>Mature_706_residues
MFEKPVVKSFQYGNHTVTLETGVMARQATAAVMATMDDTSVFVSVVAKKEAVAGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVSINPDVNPDMITMIATSAALAISGAPFNGPIGAARVGHIDG
ELVLNPSNTELENSKLDLVVSGTEGAVLMVESEADNLSEEEMLSAVVYGHDQQQVVIKAINEFAAEVATPSWNWEAPAVN
TELKAQVAELAETRLSDAYQITEKMARYEQVGAIKNDTVEALIAQDENLDEREIRGMLGSLEKNVVRSRIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQRDAQIIDSLMGEKKDNFLLHYNFPPYCVGETGFVGSPKRR
EIGHGKLAKRGIQAVMPSVDEFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
DILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILSVMDEAISGAREDISEFAPRIHTMK
ISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAATEGAAAKEAIRRIEEITAEVEVGRIYQGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVPVKVLEIDRQGRVRLSMKEAVETPAEGEAPAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=38.148667601683, Blast_Score=446, Evalue=1e-125,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=76.8451519536903, Blast_Score=1105, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=658, Percent_Identity=32.6747720364742, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17535281, Length=86, Percent_Identity=44.1860465116279, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320850, Length=101, Percent_Identity=35.6435643564356, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362905, Length=723, Percent_Identity=37.8976486860304, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651641, Length=723, Percent_Identity=37.8976486860304, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651643, Length=723, Percent_Identity=37.8976486860304, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI161079377, Length=663, Percent_Identity=38.0090497737557, Blast_Score=427, Evalue=1e-120,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76071; Mature: 76071

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKPVVKSFQYGNHTVTLETGVMARQATAAVMATMDDTSVFVSVVAKKEAVAGQDFFPL
CCCCCCHHHEECCCEEEEEECCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHHCCCCCCCCHHHHHHHEEEEEEEE
INPDVNPDMITMIATSAALAISGAPFNGPIGAARVGHIDGELVLNPSNTELENSKLDLVV
CCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCEEEEEE
SGTEGAVLMVESEADNLSEEEMLSAVVYGHDQQQVVIKAINEFAAEVATPSWNWEAPAVN
ECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TELKAQVAELAETRLSDAYQITEKMARYEQVGAIKNDTVEALIAQDENLDEREIRGMLGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHHHH
LEKNVVRSRIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQ
HHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECCCC
RDAQIIDSLMGEKKDNFLLHYNFPPYCVGETGFVGSPKRREIGHGKLAKRGIQAVMPSVD
CHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
EFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
CCCHHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCHHHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILSVMDE
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
AISGAREDISEFAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAA
HHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TEGAAAKEAIRRIEEITAEVEVGRIYQGKVARLADFGAFVTILPGKDGLVHISQIADKRV
CCCHHHHHHHHHHHHHHHHHEECCEECCHHHHHHHCCEEEEEECCCCCCEEHHHHHHHHH
EKVSDYLTEGQEVPVKVLEIDRQGRVRLSMKEAVETPAEGEAPAAE
HHHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFEKPVVKSFQYGNHTVTLETGVMARQATAAVMATMDDTSVFVSVVAKKEAVAGQDFFPL
CCCCCCHHHEECCCEEEEEECCCHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPSAFKNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHHCCCCCCCCHHHHHHHEEEEEEEE
INPDVNPDMITMIATSAALAISGAPFNGPIGAARVGHIDGELVLNPSNTELENSKLDLVV
CCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCEEEEEE
SGTEGAVLMVESEADNLSEEEMLSAVVYGHDQQQVVIKAINEFAAEVATPSWNWEAPAVN
ECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TELKAQVAELAETRLSDAYQITEKMARYEQVGAIKNDTVEALIAQDENLDEREIRGMLGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHHHH
LEKNVVRSRIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSSLFTRGETQALVTATLGTQ
HHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECCCC
RDAQIIDSLMGEKKDNFLLHYNFPPYCVGETGFVGSPKRREIGHGKLAKRGIQAVMPSVD
CHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
EFPYTVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKEGDDFVVLS
CCCHHHHHHHHHHHCCCCCHHHHHHCCHHEEHCCCCCCCHHHHHHHHHHHCCCCCEEEEH
DILGDEDHLGDMDFKVAGTNAGITALQMDIKIEGITKEIMQIALNQAQGARKHILSVMDE
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
AISGAREDISEFAPRIHTMKISSDKIKDVIGKGGAVIRALCEETGTTIEIEDDGTIKIAA
HHCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TEGAAAKEAIRRIEEITAEVEVGRIYQGKVARLADFGAFVTILPGKDGLVHISQIADKRV
CCCHHHHHHHHHHHHHHHHHEECCEECCHHHHHHHCCEEEEEECCCCCCEEHHHHHHHHH
EKVSDYLTEGQEVPVKVLEIDRQGRVRLSMKEAVETPAEGEAPAAE
HHHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA