Click here to switch to the map view.

The map label for this gene is radA [H]

Identifier: 218710419

GI number: 218710419

Start: 2624472

End: 2625851

Strand: Reverse

Name: radA [H]

Synonym: VS_2456

Alternate gene names: 218710419

Gene position: 2625851-2624472 (Counterclockwise)

Preceding gene: 218710421

Following gene: 218710418

Centisome position: 79.59

GC content: 47.97

Gene sequence:

>1380_bases
ATGGCTAAGGCAAAGCGAGCTTATGTGTGTAATGACTGTGGTGCTGACTTTCCACGTTGGCAAGGGCAGTGCAATGCATG
TGGTGCTTGGAACACGATTACAGAAGTTAGGTTAGCCGCTTCACCTCAGGTCGCGCGCAATGAACGACTAAGTGGTTACG
CTGGTGGTGCAACTGAATCTTCGGTTCAAACGCTATCGGAAATCGACTTGCAAGAGGTCCCGCGCTTTAGTAGTGGCTTC
AAAGAGCTTGATCGTGTACTTGGTGGTGGTGTTGTACCTGGCGCTGCGATTCTTATTGGTGGTAACCCTGGTGCCGGTAA
ATCAACGCTGTTATTGCAGACTATGTGTCAGCTTTCTTCCCAGTTACCGACACTTTATGTCACTGGCGAAGAATCTCTTC
AACAAGTTGCGATGCGAGCATCTCGATTAGGGTTACCAAAAGAGCATCTCAAAATGCTCTCTGAAACCAATGTTGATAAA
ATCTGTCAGGTTGCAGAAAGAGAGCAGCCTAAGATCATGGTTATCGACTCAATCCAAGTTATGCATGTCGCTGACGTTCA
ATCGTCTCCTGGCAGTGTTGCTCAGGTGCGCGAATCGGCAACCGCATTAACGCGCTATGCGAAACAGAACAACGTTGCCG
TGTTCCTAGTGGGACACGTAACTAAAGACGGTACCTTAGCGGGACCAAAAGTACTTGAACACATTATTGACTGTTCGGTG
TTGTTAGATGGCGGAACGGACAGTCGTTTTAGAACCTTGCGCAGCCACAAGAACCGTTTTGGTGCAGTGAATGAGCTAGG
TGTGTTTGCCATGACAGGGCAAGGACTAAAAGAAGTTAGCAATCCATCGGCTATTTTTCTGTCTCGTGGTGAAGAAGAAA
CCTCAGGCAGTTCAGTGATGGTAGTTTGGGAAGGAACGCGTCCACTCCTTGTTGAAATCCAAGCGCTGGTGGATTATTCG
CAGCTGGCTAACCCGCGTCGTGTCGCTGTTGGTCTCGAGCAAAATCGACTGTCATTGTTACTAGCCGTGCTGCATAAACA
CGGCGGCTTACAAATGGCTGACCAAGATGTGTTTGTGAATGTCGTTGGTGGTGTTAAAGTAACCGAAACCAGTGCTGATC
TTGCATTGGTAATGGCTTTACTTTCGAGTTTCAGAGACCGCGCATTGCCAAAAGATGTGGTGGTTTTTGGCGAAGTAGGC
CTTGCTGGTGAGATTCGACCTGTACCGAGCGGTCAAGAGCGCTTAAATGAGGCGTTTAAACACGGCTTCAAGAGAGCCAT
TGTACCAGCGGCTAACATGCCTAAAGGTGGCATTCCAGGAATGCAGATCCACGGTGTGAAAAAATTATCGGAAGCAATTA
ATGCTTTTGATGAGTTGTAA

Upstream 100 bases:

>100_bases
GTATTCGTGGCCCTACTGCAACTTTTATTTTCACCTGTACGGTCAACGGGGTCACAAAATAGCAACATTTACCCGTTACT
AAGCAAATTATTGAGGTTTT

Downstream 100 bases:

>100_bases
TTGAGCTCACTTCAGCTACTCTATACAGCAGAAATTCAGTCTAACTTAAACGCTATTTTCGCTTTAAGTTAGCACTAAAC
TTAACAATCATTTAGATTGT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGYAGGATESSVQTLSEIDLQEVPRFSSGF
KELDRVLGGGVVPGAAILIGGNPGAGKSTLLLQTMCQLSSQLPTLYVTGEESLQQVAMRASRLGLPKEHLKMLSETNVDK
ICQVAEREQPKIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFLVGHVTKDGTLAGPKVLEHIIDCSV
LLDGGTDSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVSNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDYS
QLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVNVVGGVKVTETSADLALVMALLSSFRDRALPKDVVVFGEVG
LAGEIRPVPSGQERLNEAFKHGFKRAIVPAANMPKGGIPGMQIHGVKKLSEAINAFDEL

Sequences:

>Translated_459_residues
MAKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGYAGGATESSVQTLSEIDLQEVPRFSSGF
KELDRVLGGGVVPGAAILIGGNPGAGKSTLLLQTMCQLSSQLPTLYVTGEESLQQVAMRASRLGLPKEHLKMLSETNVDK
ICQVAEREQPKIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFLVGHVTKDGTLAGPKVLEHIIDCSV
LLDGGTDSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVSNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDYS
QLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVNVVGGVKVTETSADLALVMALLSSFRDRALPKDVVVFGEVG
LAGEIRPVPSGQERLNEAFKHGFKRAIVPAANMPKGGIPGMQIHGVKKLSEAINAFDEL
>Mature_458_residues
AKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGYAGGATESSVQTLSEIDLQEVPRFSSGFK
ELDRVLGGGVVPGAAILIGGNPGAGKSTLLLQTMCQLSSQLPTLYVTGEESLQQVAMRASRLGLPKEHLKMLSETNVDKI
CQVAEREQPKIMVIDSIQVMHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFLVGHVTKDGTLAGPKVLEHIIDCSVL
LDGGTDSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVSNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDYSQ
LANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVNVVGGVKVTETSADLALVMALLSSFRDRALPKDVVVFGEVGL
AGEIRPVPSGQERLNEAFKHGFKRAIVPAANMPKGGIPGMQIHGVKKLSEAINAFDEL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=81.0869565217391, Blast_Score=776, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49175; Mature: 49044

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGYAGGATES
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCHHHCCCCCCCCCCCCHH
SVQTLSEIDLQEVPRFSSGFKELDRVLGGGVVPGAAILIGGNPGAGKSTLLLQTMCQLSS
HHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
QLPTLYVTGEESLQQVAMRASRLGLPKEHLKMLSETNVDKICQVAEREQPKIMVIDSIQV
CCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEE
MHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFLVGHVTKDGTLAGPKVLEHIIDCSV
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCCEE
LLDGGTDSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVSNPSAIFLSRGEEETSGSSVM
EEECCCCHHHHHHHHHHHHCCCHHHCCEEEECCCCHHHHCCCCEEEEECCCCCCCCCEEE
VVWEGTRPLLVEIQALVDYSQLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVN
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCEEEE
VVGGVKVTETSADLALVMALLSSFRDRALPKDVVVFGEVGLAGEIRPVPSGQERLNEAFK
EECCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
HGFKRAIVPAANMPKGGIPGMQIHGVKKLSEAINAFDEL
HHHHEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAKRAYVCNDCGADFPRWQGQCNACGAWNTITEVRLAASPQVARNERLSGYAGGATES
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCHHHCCCCCCCCCCCCHH
SVQTLSEIDLQEVPRFSSGFKELDRVLGGGVVPGAAILIGGNPGAGKSTLLLQTMCQLSS
HHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
QLPTLYVTGEESLQQVAMRASRLGLPKEHLKMLSETNVDKICQVAEREQPKIMVIDSIQV
CCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEE
MHVADVQSSPGSVAQVRESATALTRYAKQNNVAVFLVGHVTKDGTLAGPKVLEHIIDCSV
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCCEE
LLDGGTDSRFRTLRSHKNRFGAVNELGVFAMTGQGLKEVSNPSAIFLSRGEEETSGSSVM
EEECCCCHHHHHHHHHHHHCCCHHHCCEEEECCCCHHHHCCCCEEEEECCCCCCCCCEEE
VVWEGTRPLLVEIQALVDYSQLANPRRVAVGLEQNRLSLLLAVLHKHGGLQMADQDVFVN
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCEEEE
VVGGVKVTETSADLALVMALLSSFRDRALPKDVVVFGEVGLAGEIRPVPSGQERLNEAFK
EECCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
HGFKRAIVPAANMPKGGIPGMQIHGVKKLSEAINAFDEL
HHHHEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]