Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 218710415

GI number: 218710415

Start: 2620738

End: 2621688

Strand: Direct

Name: gcvA [H]

Synonym: VS_2452

Alternate gene names: 218710415

Gene position: 2620738-2621688 (Clockwise)

Preceding gene: 218710413

Following gene: 218710416

Centisome position: 79.43

GC content: 43.64

Gene sequence:

>951_bases
GTGAGAGAACGAACCCCACCTTTCCAAGGGATCTACTATTTTTATACTGCGGCTGAAACAGGCAGTTTTAAACTCGCGGC
TGAAAAGCTGTTCGTCACGGCAGCTGCCGTCAGCCAACAAATTCGTCAACTTGAAGAGTGGCTAGGTGCAGACTTGTTTA
TTCGTCAGCACCGAAAAATAGTACTCACTCACGAAGGTGATGTGCTTTACCTGCAAGCTAAGAAAGGCTTTGCTCATATT
CAAGATGGGGTAAGACGAATAAACCAAGACCCCAATCCTACTCAACTGTCTATTTCAACCGTACCATCATTTGCTCAACA
CTGGTTGGTGCCTAGAATTGGAGACTTTCGCGATCGTCACCCTGATCTATCCATGTTGATTGAACCGACCAACAAGTTGG
TGACGTTTGAAGATTCTAATGTCGATGTCTGCGTTCGATATGGTCATGGTAATTATCCAAACATTGAATCACGTTGGTTA
ATGGACGAAGTGGTTTACCCTGTCTGCCACCCTATCTACCAAGAGAAGCATGGTATTTATGACATCGACGATCTACACAA
AGCAGAGCTAATTGAAGACCGATGGCCAGATATGGATTGGAATCTATGGCTAGACATTGTAGGAGCGAAGGCAGGACGCT
CATCACTGCAATTTGATGGCTCGCATTTTGTTTTAGAAGGTGCATTATCCGTACAAGGCGTTGCTCTTGTGAAGCACAGT
TTGGTCTATCGGTATTTGCAGGAAAAGAAACTCGTTCGAATCGGTAACTTAGCACTCAAGCCTAAATACAATTACTTCTT
ATGTGCCCCTGCTGGCTACTTTCATCGAGAAAAGATAAAGCGTTTTGAGGCTTGGATGCAAAATCAGGTTCGGTTGTTTG
GGAATAGAGGTCGAGAAGAACTAACTATTATCGAGACAGATTACCAGCTTAAATGGTCTGATAATTCATAA

Upstream 100 bases:

>100_bases
CTATCTCCTGTTGATTTTTCGCTTTACCATGGTTAATAATCAACCAATAGATAAATATCGACAAACGATAGATACTGACA
TTCAGTTAAGGAAAACTAAT

Downstream 100 bases:

>100_bases
AGCGAAACTGGTATACTTAGCTCAAATAAATGGCGAGAACATCTCATGACACAAGACAATAATCCACTTCACGGTATCAC
TCTACAGAAGCTACTGACTG

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRERTPPFQGIYYFYTAAETGSFKLAAEKLFVTAAAVSQQIRQLEEWLGADLFIRQHRKIVLTHEGDVLYLQAKKGFAHI
QDGVRRINQDPNPTQLSISTVPSFAQHWLVPRIGDFRDRHPDLSMLIEPTNKLVTFEDSNVDVCVRYGHGNYPNIESRWL
MDEVVYPVCHPIYQEKHGIYDIDDLHKAELIEDRWPDMDWNLWLDIVGAKAGRSSLQFDGSHFVLEGALSVQGVALVKHS
LVYRYLQEKKLVRIGNLALKPKYNYFLCAPAGYFHREKIKRFEAWMQNQVRLFGNRGREELTIIETDYQLKWSDNS

Sequences:

>Translated_316_residues
MRERTPPFQGIYYFYTAAETGSFKLAAEKLFVTAAAVSQQIRQLEEWLGADLFIRQHRKIVLTHEGDVLYLQAKKGFAHI
QDGVRRINQDPNPTQLSISTVPSFAQHWLVPRIGDFRDRHPDLSMLIEPTNKLVTFEDSNVDVCVRYGHGNYPNIESRWL
MDEVVYPVCHPIYQEKHGIYDIDDLHKAELIEDRWPDMDWNLWLDIVGAKAGRSSLQFDGSHFVLEGALSVQGVALVKHS
LVYRYLQEKKLVRIGNLALKPKYNYFLCAPAGYFHREKIKRFEAWMQNQVRLFGNRGREELTIIETDYQLKWSDNS
>Mature_316_residues
MRERTPPFQGIYYFYTAAETGSFKLAAEKLFVTAAAVSQQIRQLEEWLGADLFIRQHRKIVLTHEGDVLYLQAKKGFAHI
QDGVRRINQDPNPTQLSISTVPSFAQHWLVPRIGDFRDRHPDLSMLIEPTNKLVTFEDSNVDVCVRYGHGNYPNIESRWL
MDEVVYPVCHPIYQEKHGIYDIDDLHKAELIEDRWPDMDWNLWLDIVGAKAGRSSLQFDGSHFVLEGALSVQGVALVKHS
LVYRYLQEKKLVRIGNLALKPKYNYFLCAPAGYFHREKIKRFEAWMQNQVRLFGNRGREELTIIETDYQLKWSDNS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=32.9896907216495, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=32.0689655172414, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=29.0657439446367, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI157672245, Length=190, Percent_Identity=32.6315789473684, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786401, Length=177, Percent_Identity=27.683615819209, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788887, Length=178, Percent_Identity=32.5842696629214, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693193, Length=167, Percent_Identity=27.5449101796407, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36787; Mature: 36787

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERTPPFQGIYYFYTAAETGSFKLAAEKLFVTAAAVSQQIRQLEEWLGADLFIRQHRKI
CCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCEE
VLTHEGDVLYLQAKKGFAHIQDGVRRINQDPNPTQLSISTVPSFAQHWLVPRIGDFRDRH
EEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCCC
PDLSMLIEPTNKLVTFEDSNVDVCVRYGHGNYPNIESRWLMDEVVYPVCHPIYQEKHGIY
CCCEEEEECCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DIDDLHKAELIEDRWPDMDWNLWLDIVGAKAGRSSLQFDGSHFVLEGALSVQGVALVKHS
CCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEEECCCCCHHHHHHHH
LVYRYLQEKKLVRIGNLALKPKYNYFLCAPAGYFHREKIKRFEAWMQNQVRLFGNRGREE
HHHHHHHHCCCEEECCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LTIIETDYQLKWSDNS
EEEEEECEEEEECCCC
>Mature Secondary Structure
MRERTPPFQGIYYFYTAAETGSFKLAAEKLFVTAAAVSQQIRQLEEWLGADLFIRQHRKI
CCCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCEE
VLTHEGDVLYLQAKKGFAHIQDGVRRINQDPNPTQLSISTVPSFAQHWLVPRIGDFRDRH
EEEECCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCCC
PDLSMLIEPTNKLVTFEDSNVDVCVRYGHGNYPNIESRWLMDEVVYPVCHPIYQEKHGIY
CCCEEEEECCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
DIDDLHKAELIEDRWPDMDWNLWLDIVGAKAGRSSLQFDGSHFVLEGALSVQGVALVKHS
CCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEEECCCCCHHHHHHHH
LVYRYLQEKKLVRIGNLALKPKYNYFLCAPAGYFHREKIKRFEAWMQNQVRLFGNRGREE
HHHHHHHHCCCEEECCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LTIIETDYQLKWSDNS
EEEEEECEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]