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The map label for this gene is queF

Identifier: 218710354

GI number: 218710354

Start: 2556735

End: 2557580

Strand: Direct

Name: queF

Synonym: VS_2390

Alternate gene names: 218710354

Gene position: 2556735-2557580 (Clockwise)

Preceding gene: 218710350

Following gene: 218710355

Centisome position: 77.49

GC content: 43.74

Gene sequence:

>846_bases
ATGAGCAAATATTCTGACGCAAAAGAGCTAGCGGGTTTAACCCTAGGCCAAAAAACTGAGTACTCTAACCAATACGATGC
AAGTTTATTGCAACCAGTACCTCGTAGCCTTAATCGTAATGATCTTGCGCTAAACGGAGAGTTACCTTTTGTTGGTCATG
ATATTTGGACGATGTACGAGCTTTCGTGGCTAAACACCAATGGCTTACCACAAGTGGCTGTTGGTGAAGTGTTCATTCCG
GCAACTAGCCAAAATTTGATTGAGTCAAAATCTTTCAAGCTGTACCTAAACAGCTACAACCAAACTCAGTTTGAAAACTG
GGATCAAGTCACTGAGCGTCTAACTCAAGATTTGTCAGCATGTGCAGGCGAATCTGTGATTGTGAATGTAAATTCAGTAA
CCGATTACACCAACCAACCTATCGTGACGATGGAAGGTGACTGTATCGACAATCAAGACATCCAAATCACGAGCTATGAT
TTTGAAGCGTCACTGCTTGAAGGGGCTGCTGGCGAGCAAGAAGTAGAAGAAACTCTACATAGTCACTTATTGAAGTCGAA
CTGCTTAATCACCAATCAACCTGACTGGGGCAGCGTAGAGATCGCATATTCCGGTAAGCAAATTGACCGTGAAGCTCTGC
TACGCTATTTAGTTTCTTTCCGTGAGCACAATGAATTCCACGAACAGTGTGTTGAGCGTATCTTCACTGACATCATGAAA
TACTGTGCACCATCGAAGCTAACCGTATTTGCACGTTACACGCGTCGTGGCGGTCTGGATATCAATCCATATCGTTCAAC
TGAGCAAGATAGACCAAGCCACAATAAGCGTATGGCTCGCCAATAA

Upstream 100 bases:

>100_bases
AATCCGAGCCCTTTCCGTGCCGATTAAGCGATGACTTAATGACAGGGTAATGGGGATTCAGTAAAATCGACCCCATTCTA
ATCGAATTTGAAACGAAAGT

Downstream 100 bases:

>100_bases
TCGGCGTGAACCGCCCACACTTATTTGAACCTACAAAATCTTTAAAGGGACATTGAAATGCGTTGGTTATACGTTGTTAG
TCTATTCATTTTAAGCTTAA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSKYSDAKELAGLTLGQKTEYSNQYDASLLQPVPRSLNRNDLALNGELPFVGHDIWTMYELSWLNTNGLPQVAVGEVFIP
ATSQNLIESKSFKLYLNSYNQTQFENWDQVTERLTQDLSACAGESVIVNVNSVTDYTNQPIVTMEGDCIDNQDIQITSYD
FEASLLEGAAGEQEVEETLHSHLLKSNCLITNQPDWGSVEIAYSGKQIDREALLRYLVSFREHNEFHEQCVERIFTDIMK
YCAPSKLTVFARYTRRGGLDINPYRSTEQDRPSHNKRMARQ

Sequences:

>Translated_281_residues
MSKYSDAKELAGLTLGQKTEYSNQYDASLLQPVPRSLNRNDLALNGELPFVGHDIWTMYELSWLNTNGLPQVAVGEVFIP
ATSQNLIESKSFKLYLNSYNQTQFENWDQVTERLTQDLSACAGESVIVNVNSVTDYTNQPIVTMEGDCIDNQDIQITSYD
FEASLLEGAAGEQEVEETLHSHLLKSNCLITNQPDWGSVEIAYSGKQIDREALLRYLVSFREHNEFHEQCVERIFTDIMK
YCAPSKLTVFARYTRRGGLDINPYRSTEQDRPSHNKRMARQ
>Mature_280_residues
SKYSDAKELAGLTLGQKTEYSNQYDASLLQPVPRSLNRNDLALNGELPFVGHDIWTMYELSWLNTNGLPQVAVGEVFIPA
TSQNLIESKSFKLYLNSYNQTQFENWDQVTERLTQDLSACAGESVIVNVNSVTDYTNQPIVTMEGDCIDNQDIQITSYDF
EASLLEGAAGEQEVEETLHSHLLKSNCLITNQPDWGSVEIAYSGKQIDREALLRYLVSFREHNEFHEQCVERIFTDIMKY
CAPSKLTVFARYTRRGGLDINPYRSTEQDRPSHNKRMARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=283, Percent_Identity=63.2508833922262, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_VIBSL (B7VIV4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417975.1
- ProteinModelPortal:   B7VIV4
- SMR:   B7VIV4
- GeneID:   7161928
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2390
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32026; Mature: 31895

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYSDAKELAGLTLGQKTEYSNQYDASLLQPVPRSLNRNDLALNGELPFVGHDIWTMYE
CCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCEECCCCEEEEE
LSWLNTNGLPQVAVGEVFIPATSQNLIESKSFKLYLNSYNQTQFENWDQVTERLTQDLSA
EEEECCCCCCCEEECCEEECCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CAGESVIVNVNSVTDYTNQPIVTMEGDCIDNQDIQITSYDFEASLLEGAAGEQEVEETLH
HCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHHH
SHLLKSNCLITNQPDWGSVEIAYSGKQIDREALLRYLVSFREHNEFHEQCVERIFTDIMK
HHHHHCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
YCAPSKLTVFARYTRRGGLDINPYRSTEQDRPSHNKRMARQ
HCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SKYSDAKELAGLTLGQKTEYSNQYDASLLQPVPRSLNRNDLALNGELPFVGHDIWTMYE
CCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCEECCCCEEEEE
LSWLNTNGLPQVAVGEVFIPATSQNLIESKSFKLYLNSYNQTQFENWDQVTERLTQDLSA
EEEECCCCCCCEEECCEEECCCCHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CAGESVIVNVNSVTDYTNQPIVTMEGDCIDNQDIQITSYDFEASLLEGAAGEQEVEETLH
HCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHHH
SHLLKSNCLITNQPDWGSVEIAYSGKQIDREALLRYLVSFREHNEFHEQCVERIFTDIMK
HHHHHCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
YCAPSKLTVFARYTRRGGLDINPYRSTEQDRPSHNKRMARQ
HCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA