Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 218710285

GI number: 218710285

Start: 2486180

End: 2486836

Strand: Direct

Name: purN [H]

Synonym: VS_2319

Alternate gene names: 218710285

Gene position: 2486180-2486836 (Clockwise)

Preceding gene: 218710284

Following gene: 218710288

Centisome position: 75.35

GC content: 43.84

Gene sequence:

>657_bases
ATGAATAACAGTCACTCTAAGAAAAACATCGTCGTTTTAGTTTCAGGAAGCGGAAGTAACTTGCAAGCAATTTTGGACGC
CTGCGATAGCAATATGATTGATGCTTCAGTTAAAGCTGTCTTCTCAAACAAAGCAGAGGCTTTTGGTCTAGAGAGAGCAA
AATCAGCAGGTGTTGACGCACATTCTGTGAACCCAAAAAACTATGGTTCACGTGAAGAGTTCGATCATGAATTGATGATT
CAGATCGATGCTTACCAACCCGATTTAATCGTACTTGCGGGATACATGCGTATCTTGAGTTCAGAATTTGTTCGTCACTA
TGCCGGTAAAATGGTCAACATCCACCCTTCCCTGTTACCTAAATACCCAGGCCTACACACCCACCAGCGTGCTATTGATG
CACAAGACAAAGAGCACGGTACAAGCGTCCACTTTGTTACCGAAGAACTTGATGGTGGCCCTGTAATCTTGCAAGCCAAG
GTGCCGGTATTTGAAGATGATGATGCTGACATGTTAGCAAGCCGAGTACTGACTCAAGAGCACTGTATTTACCCGATGGT
TTGTAAATGGTTTGCTGAAGATCGTTTATCGATGGTAAACGGGAAGGCTGTCTTAGACGGTGAAACATTAGGTACACACG
GTTACGCAGAAGAGTAA

Upstream 100 bases:

>100_bases
TTTGCTATTTGGCCCACAGAAAACCTTAGTTTATGTAGGTACATTGTAAACAGTTCAGTTAATCAATCGCATGACCCACT
CTATGAAAAACAGCCATTCT

Downstream 100 bases:

>100_bases
TTAATAAGGTTGTTCTCCCAAAGTAAATCACGGAAGAATAAGCCTTAAAAACAGAAAACAAAAAGGCCGCTCACTGACTT
ATTAGTACAGTAAGCGGCCT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNNSHSKKNIVVLVSGSGSNLQAILDACDSNMIDASVKAVFSNKAEAFGLERAKSAGVDAHSVNPKNYGSREEFDHELMI
QIDAYQPDLIVLAGYMRILSSEFVRHYAGKMVNIHPSLLPKYPGLHTHQRAIDAQDKEHGTSVHFVTEELDGGPVILQAK
VPVFEDDDADMLASRVLTQEHCIYPMVCKWFAEDRLSMVNGKAVLDGETLGTHGYAEE

Sequences:

>Translated_218_residues
MNNSHSKKNIVVLVSGSGSNLQAILDACDSNMIDASVKAVFSNKAEAFGLERAKSAGVDAHSVNPKNYGSREEFDHELMI
QIDAYQPDLIVLAGYMRILSSEFVRHYAGKMVNIHPSLLPKYPGLHTHQRAIDAQDKEHGTSVHFVTEELDGGPVILQAK
VPVFEDDDADMLASRVLTQEHCIYPMVCKWFAEDRLSMVNGKAVLDGETLGTHGYAEE
>Mature_218_residues
MNNSHSKKNIVVLVSGSGSNLQAILDACDSNMIDASVKAVFSNKAEAFGLERAKSAGVDAHSVNPKNYGSREEFDHELMI
QIDAYQPDLIVLAGYMRILSSEFVRHYAGKMVNIHPSLLPKYPGLHTHQRAIDAQDKEHGTSVHFVTEELDGGPVILQAK
VPVFEDDDADMLASRVLTQEHCIYPMVCKWFAEDRLSMVNGKAVLDGETLGTHGYAEE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=197, Percent_Identity=41.1167512690355, Blast_Score=159, Evalue=1e-39,
Organism=Homo sapiens, GI209869995, Length=197, Percent_Identity=41.1167512690355, Blast_Score=159, Evalue=1e-39,
Organism=Homo sapiens, GI209869993, Length=197, Percent_Identity=41.1167512690355, Blast_Score=159, Evalue=1e-39,
Organism=Escherichia coli, GI1788846, Length=210, Percent_Identity=63.3333333333333, Blast_Score=286, Evalue=6e-79,
Organism=Escherichia coli, GI1787483, Length=156, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=176, Percent_Identity=34.0909090909091, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=30.1020408163265, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24582400, Length=208, Percent_Identity=45.6730769230769, Blast_Score=189, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23998; Mature: 23998

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSHSKKNIVVLVSGSGSNLQAILDACDSNMIDASVKAVFSNKAEAFGLERAKSAGVDA
CCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
HSVNPKNYGSREEFDHELMIQIDAYQPDLIVLAGYMRILSSEFVRHYAGKMVNIHPSLLP
CCCCCCCCCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHCC
KYPGLHTHQRAIDAQDKEHGTSVHFVTEELDGGPVILQAKVPVFEDDDADMLASRVLTQE
CCCCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCEECCCHHHHHHHHHHHH
HCIYPMVCKWFAEDRLSMVNGKAVLDGETLGTHGYAEE
CCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNNSHSKKNIVVLVSGSGSNLQAILDACDSNMIDASVKAVFSNKAEAFGLERAKSAGVDA
CCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
HSVNPKNYGSREEFDHELMIQIDAYQPDLIVLAGYMRILSSEFVRHYAGKMVNIHPSLLP
CCCCCCCCCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHCC
KYPGLHTHQRAIDAQDKEHGTSVHFVTEELDGGPVILQAKVPVFEDDDADMLASRVLTQE
CCCCCCCHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCEECCCHHHHHHHHHHHH
HCIYPMVCKWFAEDRLSMVNGKAVLDGETLGTHGYAEE
CCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]