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The map label for this gene is glnS

Identifier: 218710226

GI number: 218710226

Start: 2425586

End: 2427253

Strand: Reverse

Name: glnS

Synonym: VS_2259

Alternate gene names: 218710226

Gene position: 2427253-2425586 (Counterclockwise)

Preceding gene: 218710227

Following gene: 218710224

Centisome position: 73.57

GC content: 44.72

Gene sequence:

>1668_bases
ATGAGTGAAGCTGAGGCTCGTCCATCGAATTTCATTCGCCAAATTATCGATAAAGATTTAGCGGATGGTACACACAGTAG
CGTGCATACTCGTTTCCCGCCGGAGCCAAATGGCTACCTGCACATTGGTCACGCTAAATCTATTTGCTTGAACTTTGGTA
TTGCTCAGGACTACCAGGGACAATGTAATCTTCGTTTCGATGATACAAACCCTGAAAAAGAAGACGTTGAATACGTTGAG
TCAATTAAGAATGATGTAAGCTGGTTAGGCTTTGAATGGTCTGGTGATATTTGTTACTCATCAAACTACTTCGATACGCT
TTACGCTTATGCTGTGGAATTAATTAATAAAGGCTTAGCGTACGTTGACGAGCTAAGTCCTGATCAAATCCGTGAGTACC
GTGGCACGCTAAAAGAGCCAGGTAAAGCGAGCCCATACCGTGATCGCAGCCCTGAAGAGAACTTAGCGTTGTTCGAAAAA
ATGCGTGACGGTGGCTTTGAAGAAGGTAAAGCGTGTCTACGTGCTAAGATCGACATGAGCTCTTCATTCATGGTTATGCG
CGATCCTGTTATCTACCGTGTTCGTTTTGCTCACCACCATCAGACTGCAGACAAGTGGTGCATTTACCCAATGTACGACT
TCACTCACTGTATCTCTGATGCGCTAGAGGGTATTACGCACTCTATCTGTACGCTTGAGTTCCAAGACAACCGTCGTTTG
TATGACTGGGTTCTAGATAACATCACGATTGATTGCCAACCTCGTCAATACGAGTTTAGCCGTCTGAATCTTGAATACAC
GGTAATGTCGAAGCGTAAGCTCAACCAACTTGTGGTTGAAAACCTAGTTGAAGGTTGGGACGATCCACGTATGCCAACTA
TCTCTGGTTTGCGTCGTCGTGGCTTCACTTCAAGCTCGATTCGTGAATTCTGTAAACGTATTGGTGTGACTAAGCAAGAG
AACATGATTGAGTTCGGTTCACTTGAATCTTGTATTCGTGATGATCTGAACGAAAATGCACCTCGTGCAATGGCTGTTCT
TGACCCTATCAAGGTCGTGATTGAAAACTACGACGCTGATACCGTAGAAACATTGACGGTAGCAAACCACCCGAACAAGC
CAGAAATGGGTACTCGTGAAGTACCATTTACACGTGAAGTTTGGATTGAGCGTGATGACTTCCGTGAAGAAGCAAACAAA
AAGTACAAGCGTTTGGTTCTAGGTAAAGAAGTTCGTCTACGTGGCGCTTACGTGATCAAAGCTGAACGTATCGAAAAAGA
TGCAGAAGGCAACATTACGACTATCTTCTGTTCTTACGACAATGAAACATTGGGTAAGAACCCTGCAGATGGGCGTAAAG
TGAAAGGCGTTATTCACTGGGTATCTGCTGATAAAGCGTTGCCTGCTGAGATTCGTTTATACGATCGTCTATTCACAGTG
CCAAACCCTGCTGCTGCAGATGATTTCGCTGCAACGCTAAACCCTGAATCACTTGTTACGCTAAACGGTTTTGTTGAACC
TAGCCTAGCAGAAGGTGTGGCAGAGCAAGCGTATCAGTTTGAACGTACAGGTTATTTCTGTGTAGATTCGAAAGACTCTA
AAGCAGACGCGCTAGTATTCAACCGCACGGTTGGTCTACGTGACACTTGGGGTAAAACTGAGGCTTAA

Upstream 100 bases:

>100_bases
CAATGATCTTTCTATCTTTGACAGGCGCTGTAAGCGACCATTTTCATTGGTACAAAAGATAACTTTCTCTGACAATACTA
AATACATAGAGGTGCTATAG

Downstream 100 bases:

>100_bases
TGGCTTTGTTTTTCTCTACTCTATTTTTCCAAGCTCGGTTTCTCGGAGCTTAGTTTCTCAAGAGACGCTTAATAAAAAAC
CGGCTTAGAGCCGGTTTTTT

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSEAEARPSNFIRQIIDKDLADGTHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKEDVEYVE
SIKNDVSWLGFEWSGDICYSSNYFDTLYAYAVELINKGLAYVDELSPDQIREYRGTLKEPGKASPYRDRSPEENLALFEK
MRDGGFEEGKACLRAKIDMSSSFMVMRDPVIYRVRFAHHHQTADKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLDNITIDCQPRQYEFSRLNLEYTVMSKRKLNQLVVENLVEGWDDPRMPTISGLRRRGFTSSSIREFCKRIGVTKQE
NMIEFGSLESCIRDDLNENAPRAMAVLDPIKVVIENYDADTVETLTVANHPNKPEMGTREVPFTREVWIERDDFREEANK
KYKRLVLGKEVRLRGAYVIKAERIEKDAEGNITTIFCSYDNETLGKNPADGRKVKGVIHWVSADKALPAEIRLYDRLFTV
PNPAAADDFAATLNPESLVTLNGFVEPSLAEGVAEQAYQFERTGYFCVDSKDSKADALVFNRTVGLRDTWGKTEA

Sequences:

>Translated_555_residues
MSEAEARPSNFIRQIIDKDLADGTHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKEDVEYVE
SIKNDVSWLGFEWSGDICYSSNYFDTLYAYAVELINKGLAYVDELSPDQIREYRGTLKEPGKASPYRDRSPEENLALFEK
MRDGGFEEGKACLRAKIDMSSSFMVMRDPVIYRVRFAHHHQTADKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
YDWVLDNITIDCQPRQYEFSRLNLEYTVMSKRKLNQLVVENLVEGWDDPRMPTISGLRRRGFTSSSIREFCKRIGVTKQE
NMIEFGSLESCIRDDLNENAPRAMAVLDPIKVVIENYDADTVETLTVANHPNKPEMGTREVPFTREVWIERDDFREEANK
KYKRLVLGKEVRLRGAYVIKAERIEKDAEGNITTIFCSYDNETLGKNPADGRKVKGVIHWVSADKALPAEIRLYDRLFTV
PNPAAADDFAATLNPESLVTLNGFVEPSLAEGVAEQAYQFERTGYFCVDSKDSKADALVFNRTVGLRDTWGKTEA
>Mature_554_residues
SEAEARPSNFIRQIIDKDLADGTHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQGQCNLRFDDTNPEKEDVEYVES
IKNDVSWLGFEWSGDICYSSNYFDTLYAYAVELINKGLAYVDELSPDQIREYRGTLKEPGKASPYRDRSPEENLALFEKM
RDGGFEEGKACLRAKIDMSSSFMVMRDPVIYRVRFAHHHQTADKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRLY
DWVLDNITIDCQPRQYEFSRLNLEYTVMSKRKLNQLVVENLVEGWDDPRMPTISGLRRRGFTSSSIREFCKRIGVTKQEN
MIEFGSLESCIRDDLNENAPRAMAVLDPIKVVIENYDADTVETLTVANHPNKPEMGTREVPFTREVWIERDDFREEANKK
YKRLVLGKEVRLRGAYVIKAERIEKDAEGNITTIFCSYDNETLGKNPADGRKVKGVIHWVSADKALPAEIRLYDRLFTVP
NPAAADDFAATLNPESLVTLNGFVEPSLAEGVAEQAYQFERTGYFCVDSKDSKADALVFNRTVGLRDTWGKTEA

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI4826960, Length=536, Percent_Identity=46.8283582089552, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI62241042, Length=536, Percent_Identity=33.955223880597, Blast_Score=282, Evalue=6e-76,
Organism=Escherichia coli, GI1786895, Length=553, Percent_Identity=80.6509945750452, Blast_Score=961, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=210, Percent_Identity=27.6190476190476, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=530, Percent_Identity=42.6415094339623, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17511007, Length=521, Percent_Identity=33.5892514395393, Blast_Score=265, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=576, Percent_Identity=42.1875, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321192, Length=529, Percent_Identity=35.1606805293006, Blast_Score=308, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17864488, Length=536, Percent_Identity=43.0970149253731, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24649466, Length=515, Percent_Identity=34.7572815533981, Blast_Score=268, Evalue=9e-72,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYQ_VIBSL (B7VIH6)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417847.1
- GeneID:   7161801
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2259
- HOGENOM:   HBG334108
- OMA:   RMPTIAG
- ProtClustDB:   PRK05347
- GO:   GO:0005737
- HAMAP:   MF_00126
- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119:SF3
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00440

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.18

Molecular weight: Translated: 63534; Mature: 63403

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAEARPSNFIRQIIDKDLADGTHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKEDVEYVESIKNDVSWLGFEWSGDICYSSNYFDTLYAYAVELINKGLA
CEEEEECCCCCCHHHHHHHHHHHCCHHEEEEEECCCEEECCCCHHHHHHHHHHHHHCCHH
YVDELSPDQIREYRGTLKEPGKASPYRDRSPEENLALFEKMRDGGFEEGKACLRAKIDMS
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHEEEECCC
SSFMVMRDPVIYRVRFAHHHQTADKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCEE
YDWVLDNITIDCQPRQYEFSRLNLEYTVMSKRKLNQLVVENLVEGWDDPRMPTISGLRRR
EHHHHCCEEEECCCCCEEHEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GFTSSSIREFCKRIGVTKQENMIEFGSLESCIRDDLNENAPRAMAVLDPIKVVIENYDAD
CCCHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
TVETLTVANHPNKPEMGTREVPFTREVWIERDDFREEANKKYKRLVLGKEVRLRGAYVIK
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECEEEEEE
AERIEKDAEGNITTIFCSYDNETLGKNPADGRKVKGVIHWVSADKALPAEIRLYDRLFTV
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHEEEHHHCCC
PNPAAADDFAATLNPESLVTLNGFVEPSLAEGVAEQAYQFERTGYFCVDSKDSKADALVF
CCCCCCCCHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEE
NRTVGLRDTWGKTEA
ECCCCCCCCCCCCCC
>Mature Secondary Structure 
SEAEARPSNFIRQIIDKDLADGTHSSVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYQG
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCHHEEHEECCCCCCCCC
QCNLRFDDTNPEKEDVEYVESIKNDVSWLGFEWSGDICYSSNYFDTLYAYAVELINKGLA
CEEEEECCCCCCHHHHHHHHHHHCCHHEEEEEECCCEEECCCCHHHHHHHHHHHHHCCHH
YVDELSPDQIREYRGTLKEPGKASPYRDRSPEENLALFEKMRDGGFEEGKACLRAKIDMS
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHEEEECCC
SSFMVMRDPVIYRVRFAHHHQTADKWCIYPMYDFTHCISDALEGITHSICTLEFQDNRRL
CCEEEEECCEEEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEECCCCEE
YDWVLDNITIDCQPRQYEFSRLNLEYTVMSKRKLNQLVVENLVEGWDDPRMPTISGLRRR
EHHHHCCEEEECCCCCEEHEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GFTSSSIREFCKRIGVTKQENMIEFGSLESCIRDDLNENAPRAMAVLDPIKVVIENYDAD
CCCHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
TVETLTVANHPNKPEMGTREVPFTREVWIERDDFREEANKKYKRLVLGKEVRLRGAYVIK
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECEEEEEE
AERIEKDAEGNITTIFCSYDNETLGKNPADGRKVKGVIHWVSADKALPAEIRLYDRLFTV
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHEEEHHHCCC
PNPAAADDFAATLNPESLVTLNGFVEPSLAEGVAEQAYQFERTGYFCVDSKDSKADALVF
CCCCCCCCHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEE
NRTVGLRDTWGKTEA
ECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA