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The map label for this gene is sucB [H]

Identifier: 218710210

GI number: 218710210

Start: 2408945

End: 2410150

Strand: Reverse

Name: sucB [H]

Synonym: VS_2242

Alternate gene names: 218710210

Gene position: 2410150-2408945 (Counterclockwise)

Preceding gene: 218710211

Following gene: 218710209

Centisome position: 73.05

GC content: 47.84

Gene sequence:

>1206_bases
ATGACAATTGAAATTCTGGTTCCAGATTTACCTGAATCTGTGGCTGATGCAACAGTTGCTACTTGGCACAAAAAACCGGG
CGAAGCGGTTGCACGTGATGAAGTCATTGTAGACATCGAAACAGATAAAGTAGTTCTAGAAGTACCGGCTCCTGAAGCGG
GTGTTCTGGAAGCTATCATTGAAGAAGAGGGTGCTACGGTACTTTCTAAGCAGCTTCTTGCGAAAATCAAACCTGGTGCT
GTCGCTGGTGAACCAACGAAAGACACGACTGAAGATACAGAAGCTTCTCCTGATAAGCGCCACAAAGCGGCACTGACAGA
AGAAAGCAACGACGCACTAAGCCCTGCTGTTCGTCGTCTTCTTGCAGAGCACAACCTACAACCAGCTGACGTTAAAGGCA
CTGGTGTTGGTGGTCGTATTACTCGTGAGGACATCGATGCACACCTAGCAGCAGCGAAAGCGGCTCCGGCAGCATCTGCA
CCAGCAGTTGAAGCGCCAGCAGCGGCTCGCAGCCAAAAACGCGTGCCTATGACTCGCCTACGTAAGACAGTTGCAAACCG
TCTTCTAGAAGCGAAGAACAGCACAGCAATGCTGACTACTTTCAACGAAGTGAACATGAAGCCAATCATGGACCTTCGTA
AGCAGTACAAAGACCAATTCGAGAAGCGTCACGATACGCGTCTTGGTTTCATGTCTTTCTACGTGAAAGCAGTAACAGAA
GCACTGAAGCGTTTCCCAGAAGTTAACGCTTCTATCGATGGTACAGATATCGTTTACCACAACTACTTCGACATCAGCAT
GGCGGTATCTACGCCACGTGGTTTAGTGACTCCAGTACTGAAAGACTGTGACACACTAGGTTTCGCAGATATTGAAAAAG
GCATCAAAGAGCTAGCAATCAAAGGCCGTGACGGCAAGCTAACTGTTGATGAGTTGATGGGCGGTAACTTTACTATCACG
AACGGTGGTGTATTTGGTTCTCTAATGTCTACGCCAATCATCAACCCGCCTCAAGCGGCAATTTTGGGTATGCACAAAAT
CCAAGATCGTCCAATGGCTGTTGATGGCAAGGTTGAGATCTTACCAATGATGTACCTAGCGCTTTCTTACGATCACCGTC
TAATCGACGGTCGAGAATCAGTTGGCTTCCTAGTGACAATCAAAGAGCTCCTGGAAGATCCTGCACGTCTGCTATTAGAC
GTTTAG

Upstream 100 bases:

>100_bases
AGCTGTTGGCTATATGTCGGTACACTTGAAACAACAAAAGGCGTTAGTTGACGACGCTTTGACCCTACTTAAGAACTAGA
AGTAAAAGGAAAATACGCAC

Downstream 100 bases:

>100_bases
TATCTCTAAAACGTCTAGTTAACCCGGTTAGCTAGACGTAAAAAGTTTCAAAGGCTAGGCTGCTCAACGTCTGGCCTTCA
ATTGAACTGTAAAGCCATTA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTIEILVPDLPESVADATVATWHKKPGEAVARDEVIVDIETDKVVLEVPAPEAGVLEAIIEEEGATVLSKQLLAKIKPGA
VAGEPTKDTTEDTEASPDKRHKAALTEESNDALSPAVRRLLAEHNLQPADVKGTGVGGRITREDIDAHLAAAKAAPAASA
PAVEAPAAARSQKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEKRHDTRLGFMSFYVKAVTE
ALKRFPEVNASIDGTDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAIKGRDGKLTVDELMGGNFTIT
NGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLD
V

Sequences:

>Translated_401_residues
MTIEILVPDLPESVADATVATWHKKPGEAVARDEVIVDIETDKVVLEVPAPEAGVLEAIIEEEGATVLSKQLLAKIKPGA
VAGEPTKDTTEDTEASPDKRHKAALTEESNDALSPAVRRLLAEHNLQPADVKGTGVGGRITREDIDAHLAAAKAAPAASA
PAVEAPAAARSQKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEKRHDTRLGFMSFYVKAVTE
ALKRFPEVNASIDGTDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAIKGRDGKLTVDELMGGNFTIT
NGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLD
V
>Mature_400_residues
TIEILVPDLPESVADATVATWHKKPGEAVARDEVIVDIETDKVVLEVPAPEAGVLEAIIEEEGATVLSKQLLAKIKPGAV
AGEPTKDTTEDTEASPDKRHKAALTEESNDALSPAVRRLLAEHNLQPADVKGTGVGGRITREDIDAHLAAAKAAPAASAP
AVEAPAAARSQKRVPMTRLRKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEKRHDTRLGFMSFYVKAVTEA
LKRFPEVNASIDGTDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAIKGRDGKLTVDELMGGNFTITN
GGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=56.2753036437247, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=29.6983758700696, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=28.1995661605206, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=28.3813747228381, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=27.5462962962963, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=76.6749379652606, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=31.9347319347319, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=397, Percent_Identity=41.8136020151133, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=31.264367816092, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=24.220623501199, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=31.7307692307692, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=402, Percent_Identity=41.5422885572139, Blast_Score=308, Evalue=8e-85,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=27.6688453159041, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=58.8495575221239, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=28.1818181818182, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24582497, Length=290, Percent_Identity=33.448275862069, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI20129315, Length=290, Percent_Identity=33.448275862069, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43532; Mature: 43400

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILVPDLPESVADATVATWHKKPGEAVARDEVIVDIETDKVVLEVPAPEAGVLEAII
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
EEEGATVLSKQLLAKIKPGAVAGEPTKDTTEDTEASPDKRHKAALTEESNDALSPAVRRL
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHH
LAEHNLQPADVKGTGVGGRITREDIDAHLAAAKAAPAASAPAVEAPAAARSQKRVPMTRL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHH
RKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEKRHDTRLGFMSFYVKAVTE
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKRFPEVNASIDGTDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAI
HHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHEE
KGRDGKLTVDELMGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEI
CCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHH
LPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLDV
HHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TIEILVPDLPESVADATVATWHKKPGEAVARDEVIVDIETDKVVLEVPAPEAGVLEAII
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
EEEGATVLSKQLLAKIKPGAVAGEPTKDTTEDTEASPDKRHKAALTEESNDALSPAVRRL
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHH
LAEHNLQPADVKGTGVGGRITREDIDAHLAAAKAAPAASAPAVEAPAAARSQKRVPMTRL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHH
RKTVANRLLEAKNSTAMLTTFNEVNMKPIMDLRKQYKDQFEKRHDTRLGFMSFYVKAVTE
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKRFPEVNASIDGTDIVYHNYFDISMAVSTPRGLVTPVLKDCDTLGFADIEKGIKELAI
HHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHEE
KGRDGKLTVDELMGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVDGKVEI
CCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHH
LPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLDV
HHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]