Click here to switch to the map view.

The map label for this gene is pksE [H]

Identifier: 218710186

GI number: 218710186

Start: 2382360

End: 2383997

Strand: Direct

Name: pksE [H]

Synonym: VS_2218

Alternate gene names: 218710186

Gene position: 2382360-2383997 (Clockwise)

Preceding gene: 218710185

Following gene: 218710196

Centisome position: 72.21

GC content: 50.18

Gene sequence:

>1638_bases
ATGACAACTCAAACTACAATCAATAACGAAAAGCTATCTCCGTGGCCTTGGCAGATCGAAGAGAGCACAACTCGCTTTGA
TAACGCAGCAATGTCGACAATATTGAAAGACTTAAGCCTTGCTTGTTACGTCGTGAACCACCCAGAAAAAGGGTTAGGTG
TTAGCCAACAAGCAGAGATTGCATCGGGCGATTCAGCAAGCTCAGCGAACAGCCAACCTGTTAGCGCCTTTGCTCCTGCA
CTTGGTACACAAAGCCTAGGCGACGAAGATTTTCGTCGCTGTCACGGCGTGAAATACGCTTACTATGCGGGCGCAATGGC
AAACGGCATTTCATCTGAAGAGTTAGTGATTGCGCTTGGCCAAGCTGGCATTCTTTGTTCATTTGGCGCGGCGGGCTTAA
TCCCGTCTCGCGTTGAGCAAGCGATCAACCGTATTCAAGCAGCACTGCCAAACGGTCCTTATGCGTTTAACCTGATTCAC
AGCCCAAGCGAACCCGCTCTAGAGCGCGGCAGTGTAGAGCTATTTTTGAAACACAAAGTGAAAACGGTTGAGGCTTCTGC
CTTCTTAGGCCTAACACCGCAGATCGTTCACTACCGTGCCGCGGGCCTTAGCCGCGATGCACAAGGTGAGATTCAGATTG
GCAACAAGGTTATTGCTAAGGTAAGCCGTACTGAAGTTGCAAGTAAGTTCATGCAACCAGCTCCAGTTAAAATGCTGCAA
GCTTTGGTTGATGAAGGTCGAATCACAGCAGAACAGATGGAACTGGCACAGCTGGTTCCTATGGCTGATGACATTACCGC
AGAAGCCGATTCTGGTGGTCATACCGATAACCGTCCACTGGTTACCCTGCTGCCAACGATTCTTGCACTGAAAGAACAAA
CCCAAGCACAGTACCAATTCAAAACTCCGCTACGTGTCGGTTGTGGTGGTGGCGTAGGCACGCCTGACGCGGCTCTAGCG
ACGTTTAACATGGGTGCTGCTTACATTGTTACCGGTTCAATCAACCAAGCGTGTGTTGAGGCTGGCGCGAGCGAACACAC
ACGTAAGCTGCTTTCGACAACTGAAATGGCTGACGTGACTATGGCTCCGGCAGCAGACATGTTCGAAATGGGCGTGAAAC
TGCAAGTGGTTAAGCGCGGCACTTTATTCCCAATGCGTGCGAACAAGTTGTATGAACTTTACACTCGCTACGACTCAATT
GAAGCGATTCCAGTTGAAGAGCGCCAAAAGCTAGAGAAGCAGGTATTCCGTTCAACACTGGATGACATTTGGGCGGGAAC
TGTGGCGCACTTTAACGAGCGTGACCCTAAACAGATTGAACGTGCTGAAGGCAACCCAAAGCGTAAGATGGCGTTGATCT
TCCGTTGGTACTTAGGGCTTTCTAGCCGTTGGTCAAACACCGGTGAACAAGGTCGTGAAATGGATTACCAAGTATGGGCT
GGCCCGGCACTTGGCGCATTCAACGCCTGGGCGAAAGACAGCTATCTAGATGATTACCAACAGCGTAATGCCGTTGATCT
TGCTAAGCATCTGATGCACGGTGCTGCGTACTTAGCTCGTGTTAACTTGCTGACCTCACAAGGTATCAAGCTTGATCCGG
AACTAGCACGCTGGAAGCCGACGCAAAGAATGGCTTAA

Upstream 100 bases:

>100_bases
AAACCTGAGCAAAGATGGTCTACGTATTTACGAAGTGAAAGACATCGCGATTTGTATCGAAGAAGCATAAGCCTGACAGA
GAACTAGGAAAGAATTTAGT

Downstream 100 bases:

>100_bases
GTGCCGAGTAGCTAGCTAAATTTTACCAAATAAAAAAGCGACCTTAATTAGGTCGCTTTTCTTTGAATCATCGAAATGTC
ATTGCAGTCGTTGGTTATTT

Product: omega-3 polyunsaturated fatty acid synthase PfaD

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTTQTTINNEKLSPWPWQIEESTTRFDNAAMSTILKDLSLACYVVNHPEKGLGVSQQAEIASGDSASSANSQPVSAFAPA
LGTQSLGDEDFRRCHGVKYAYYAGAMANGISSEELVIALGQAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYAFNLIH
SPSEPALERGSVELFLKHKVKTVEASAFLGLTPQIVHYRAAGLSRDAQGEIQIGNKVIAKVSRTEVASKFMQPAPVKMLQ
ALVDEGRITAEQMELAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKEQTQAQYQFKTPLRVGCGGGVGTPDAALA
TFNMGAAYIVTGSINQACVEAGASEHTRKLLSTTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYELYTRYDSI
EAIPVEERQKLEKQVFRSTLDDIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNTGEQGREMDYQVWA
GPALGAFNAWAKDSYLDDYQQRNAVDLAKHLMHGAAYLARVNLLTSQGIKLDPELARWKPTQRMA

Sequences:

>Translated_545_residues
MTTQTTINNEKLSPWPWQIEESTTRFDNAAMSTILKDLSLACYVVNHPEKGLGVSQQAEIASGDSASSANSQPVSAFAPA
LGTQSLGDEDFRRCHGVKYAYYAGAMANGISSEELVIALGQAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYAFNLIH
SPSEPALERGSVELFLKHKVKTVEASAFLGLTPQIVHYRAAGLSRDAQGEIQIGNKVIAKVSRTEVASKFMQPAPVKMLQ
ALVDEGRITAEQMELAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKEQTQAQYQFKTPLRVGCGGGVGTPDAALA
TFNMGAAYIVTGSINQACVEAGASEHTRKLLSTTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYELYTRYDSI
EAIPVEERQKLEKQVFRSTLDDIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNTGEQGREMDYQVWA
GPALGAFNAWAKDSYLDDYQQRNAVDLAKHLMHGAAYLARVNLLTSQGIKLDPELARWKPTQRMA
>Mature_544_residues
TTQTTINNEKLSPWPWQIEESTTRFDNAAMSTILKDLSLACYVVNHPEKGLGVSQQAEIASGDSASSANSQPVSAFAPAL
GTQSLGDEDFRRCHGVKYAYYAGAMANGISSEELVIALGQAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYAFNLIHS
PSEPALERGSVELFLKHKVKTVEASAFLGLTPQIVHYRAAGLSRDAQGEIQIGNKVIAKVSRTEVASKFMQPAPVKMLQA
LVDEGRITAEQMELAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKEQTQAQYQFKTPLRVGCGGGVGTPDAALAT
FNMGAAYIVTGSINQACVEAGASEHTRKLLSTTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYELYTRYDSIE
AIPVEERQKLEKQVFRSTLDDIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNTGEQGREMDYQVWAG
PALGAFNAWAKDSYLDDYQQRNAVDLAKHLMHGAAYLARVNLLTSQGIKLDPELARWKPTQRMA

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 59512; Mature: 59381

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTTINNEKLSPWPWQIEESTTRFDNAAMSTILKDLSLACYVVNHPEKGLGVSQQAEI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCHHC
ASGDSASSANSQPVSAFAPALGTQSLGDEDFRRCHGVKYAYYAGAMANGISSEELVIALG
CCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEC
QAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYAFNLIHSPSEPALERGSVELFLKHKV
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEEEHHH
KTVEASAFLGLTPQIVHYRAAGLSRDAQGEIQIGNKVIAKVSRTEVASKFMQPAPVKMLQ
HHEEHHHHCCCCHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHH
ALVDEGRITAEQMELAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKEQTQAQYQF
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEE
KTPLRVGCGGGVGTPDAALATFNMGAAYIVTGSINQACVEAGASEHTRKLLSTTEMADVT
CCCEEEECCCCCCCCCHHHEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHEE
MAPAADMFEMGVKLQVVKRGTLFPMRANKLYELYTRYDSIEAIPVEERQKLEKQVFRSTL
CCCCHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHH
DDIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNTGEQGREMDYQVWA
HHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEC
GPALGAFNAWAKDSYLDDYQQRNAVDLAKHLMHGAAYLARVNLLTSQGIKLDPELARWKP
CCCCCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
TQRMA
HHCCC
>Mature Secondary Structure 
TTQTTINNEKLSPWPWQIEESTTRFDNAAMSTILKDLSLACYVVNHPEKGLGVSQQAEI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCHHC
ASGDSASSANSQPVSAFAPALGTQSLGDEDFRRCHGVKYAYYAGAMANGISSEELVIALG
CCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEC
QAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYAFNLIHSPSEPALERGSVELFLKHKV
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEEEHHH
KTVEASAFLGLTPQIVHYRAAGLSRDAQGEIQIGNKVIAKVSRTEVASKFMQPAPVKMLQ
HHEEHHHHCCCCHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHH
ALVDEGRITAEQMELAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKEQTQAQYQF
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEE
KTPLRVGCGGGVGTPDAALATFNMGAAYIVTGSINQACVEAGASEHTRKLLSTTEMADVT
CCCEEEECCCCCCCCCHHHEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHEE
MAPAADMFEMGVKLQVVKRGTLFPMRANKLYELYTRYDSIEAIPVEERQKLEKQVFRSTL
CCCCHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHH
DDIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNTGEQGREMDYQVWA
HHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEC
GPALGAFNAWAKDSYLDDYQQRNAVDLAKHLMHGAAYLARVNLLTSQGIKLDPELARWKP
CCCCCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
TQRMA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]