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The map label for this gene is purU [H]

Identifier: 218710196

GI number: 218710196

Start: 2392374

End: 2393207

Strand: Direct

Name: purU [H]

Synonym: VS_2228

Alternate gene names: 218710196

Gene position: 2392374-2393207 (Clockwise)

Preceding gene: 218710186

Following gene: 218710198

Centisome position: 72.51

GC content: 44.0

Gene sequence:

>834_bases
ATGGAAAGAAAAACTTTATTAACACATTGTACTGACGCCCCAGGCCTCATCTCAAAGATCACCAACATTTGTTATAAGCA
CCAACTCAATATTATTCACAATAATGAGTTCGTTGATAACACAAGTGGCCACTTCTTTATGCGCACCGAGCTAGAGGGCT
ACTTCAATGACGAAACCTTACTCGCCGATTTAGACCAAGCCCTGCCAGAAAACACAAAACGTAAGCTTGTTGATTCATCT
CGTAAGCGTGTTGTGATTCTGGTAACTAAAGAAGCACACTGCCTTGGCGATATTCTGATGAAGAACTTCGATGGCAGTTT
AGATGTTGATATTGCGGCGGTAGTTGGTAACTACGATACACTACAAAGCCTAACCGAGCGTTTTGATATCCCTTACCACC
ATGTTTCTCACGAAGGATTGAACCGTGAAGAGCATGAAAAGAAGATGTTGGAGGTGATTGACCAGTATGAGGCAGACTAC
CTGGTTCTTGCTAAATACATGCGAGTGCTGACGCCGGGGTTTGTTGAGAAGTACAACCACAAAATCATCAACATTCACCA
CAGTTTCTTGCCAGCATTCATTGGTGCTAAGCCATACCAGCAAGCTTACGAGCGCGGTGTGAAGATCATTGGTGCAACGG
CACACTTTGTGACAAACGATCTCGATGAAGGTCCAATCATCAAGCAAGATGTTATCCCAGTGGATCACAACTTCAGCGCA
AAAGACATGGCACAAGCGGGTCGTGACGTAGAGAAGAACGTGTTGAGCAAGGCTTTAAACAAAGTGATCAACGATCATGT
GTTTGTTTACGGCAACAAGACCGTAATTCTGTAG

Upstream 100 bases:

>100_bases
GTGATACTGCGGCTCAGTTCACCGTTCGACGGCCTGCATTAAATTTTGAAGAATCCCGATTTCATATAATAAGTGCAACA
TTCATGGAAGTAAGATGCAA

Downstream 100 bases:

>100_bases
GTCGGAGGTTCGATTTCCGGTAAGACTTCCAGTTAAAACCCAATAAAAAACGCGCAATAGGGTTAATCCATTGCGCGTTT
TTTGTATCTGATGATCTGAA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MERKTLLTHCTDAPGLISKITNICYKHQLNIIHNNEFVDNTSGHFFMRTELEGYFNDETLLADLDQALPENTKRKLVDSS
RKRVVILVTKEAHCLGDILMKNFDGSLDVDIAAVVGNYDTLQSLTERFDIPYHHVSHEGLNREEHEKKMLEVIDQYEADY
LVLAKYMRVLTPGFVEKYNHKIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSA
KDMAQAGRDVEKNVLSKALNKVINDHVFVYGNKTVIL

Sequences:

>Translated_277_residues
MERKTLLTHCTDAPGLISKITNICYKHQLNIIHNNEFVDNTSGHFFMRTELEGYFNDETLLADLDQALPENTKRKLVDSS
RKRVVILVTKEAHCLGDILMKNFDGSLDVDIAAVVGNYDTLQSLTERFDIPYHHVSHEGLNREEHEKKMLEVIDQYEADY
LVLAKYMRVLTPGFVEKYNHKIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSA
KDMAQAGRDVEKNVLSKALNKVINDHVFVYGNKTVIL
>Mature_277_residues
MERKTLLTHCTDAPGLISKITNICYKHQLNIIHNNEFVDNTSGHFFMRTELEGYFNDETLLADLDQALPENTKRKLVDSS
RKRVVILVTKEAHCLGDILMKNFDGSLDVDIAAVVGNYDTLQSLTERFDIPYHHVSHEGLNREEHEKKMLEVIDQYEADY
LVLAKYMRVLTPGFVEKYNHKIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSA
KDMAQAGRDVEKNVLSKALNKVINDHVFVYGNKTVIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=30.5699481865285, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=30.5699481865285, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=30.5699481865285, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=70.3971119133574, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=42.5531914893617, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=33.7349397590361, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=28.0612244897959, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31576; Mature: 31576

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKTLLTHCTDAPGLISKITNICYKHQLNIIHNNEFVDNTSGHFFMRTELEGYFNDETL
CCCCHHHHHCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEEEEECCCCCCCCHH
LADLDQALPENTKRKLVDSSRKRVVILVTKEAHCLGDILMKNFDGSLDVDIAAVVGNYDT
HHHHHHHCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCHHH
LQSLTERFDIPYHHVSHEGLNREEHEKKMLEVIDQYEADYLVLAKYMRVLTPGFVEKYNH
HHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCC
KIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSA
EEEEEHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCH
KDMAQAGRDVEKNVLSKALNKVINDHVFVYGNKTVIL
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC
>Mature Secondary Structure
MERKTLLTHCTDAPGLISKITNICYKHQLNIIHNNEFVDNTSGHFFMRTELEGYFNDETL
CCCCHHHHHCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEEEEECCCCCCCCHH
LADLDQALPENTKRKLVDSSRKRVVILVTKEAHCLGDILMKNFDGSLDVDIAAVVGNYDT
HHHHHHHCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCHHH
LQSLTERFDIPYHHVSHEGLNREEHEKKMLEVIDQYEADYLVLAKYMRVLTPGFVEKYNH
HHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCC
KIINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSA
EEEEEHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCH
KDMAQAGRDVEKNVLSKALNKVINDHVFVYGNKTVIL
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]