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The map label for this gene is leuO [H]

Identifier: 218710076

GI number: 218710076

Start: 2249474

End: 2250409

Strand: Reverse

Name: leuO [H]

Synonym: VS_2097

Alternate gene names: 218710076

Gene position: 2250409-2249474 (Counterclockwise)

Preceding gene: 218710082

Following gene: 218710075

Centisome position: 68.21

GC content: 41.13

Gene sequence:

>936_bases
ATGAGCAACGAAATCCCCAATTTCAACCTACTTGCTGTGTTTTCTGCGGTGATGGAGCAGGGCAGTTTGAGTAAAGCCGC
GGATCAACTCAGCACCAATCAATCGACCATTAGTACCGCATTGGCACGACTGAAAAAAGAGATAGGCCAAGAGCTTTTTG
TTCGTAAAGGACGAGGTGTTACGCCTACTTCTTATGCTCAAAGTTTGTATGAACAAATCAAAACGCCGATTCATGAATTG
AATGATGTATTTCAGTCGATGGCTAATTTTGATGAGCAATCCTCTGAACGTAAGTTTGTGATTTCTTCACCTGAGCACTT
ACAGTGGATTTTACTCAATAGTTTTGCTGCGCTTCCTAATCAAAATCTATCGCTAGAAGTCTTTGACCAACCAGACAGTG
ATGAGCGTATTTACGAAGACCTTTTGACTCAAGAGTTCGATGCAATGATCGATATTGTGGTACCTGAACACCCAAGCATT
GTTAGTGAAACTCTATATGAAGGCGAGTTTGTTATTGTGTGTAGAGAGGATCACCCGAGAATTCAAGGTGAAATCAGCGA
AGCGCAATTCATGAATGAAAAGCATGCCGTATTAGATAGAACACGGCGGAAAGTTCGCAGTTTAAATCACTACACATCAT
TAGATTTATCTAAGCGTAAAATCGTGTTTCACGGCCGATCACTGTTCAGTAATATCTTGCTATGTAGCCAATCGGATTAC
ATCACCGTAGTGCCTTTATCTATGGCTGTGCAGTTTCAAGAAAGGCTAGGTTTGCAGTTGTTTAAGCCACCTTTTGAATA
TCAGCCTATATCTCACTATTTGATTTGGTTAAAGAAGCAGAATAATGACCCTGCTCATAAATGGTTCCGAGAACAAGTCA
TCAGTACATCAGATGCGATGTCGAAGACGCTGAAAGATAGGCGTTTGCGGTTTTAA

Upstream 100 bases:

>100_bases
ATAATCGATGCGTTATACCTTTAACAAATGTTTTTATTGACGATATTTAATCGTTTTTACGATAGCATGGTGTTTATGGT
CGCCTAACGAGTAATGATAT

Downstream 100 bases:

>100_bases
TTGAGAGTCTAACTCGAAGGGAAGCATTGGCTTCCCTTTTTTGTGTCATTGAGTTCGTGCTAGTCATAAATTTCATAACA
AAAAATTATCACATTGAGAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSNEIPNFNLLAVFSAVMEQGSLSKAADQLSTNQSTISTALARLKKEIGQELFVRKGRGVTPTSYAQSLYEQIKTPIHEL
NDVFQSMANFDEQSSERKFVISSPEHLQWILLNSFAALPNQNLSLEVFDQPDSDERIYEDLLTQEFDAMIDIVVPEHPSI
VSETLYEGEFVIVCREDHPRIQGEISEAQFMNEKHAVLDRTRRKVRSLNHYTSLDLSKRKIVFHGRSLFSNILLCSQSDY
ITVVPLSMAVQFQERLGLQLFKPPFEYQPISHYLIWLKKQNNDPAHKWFREQVISTSDAMSKTLKDRRLRF

Sequences:

>Translated_311_residues
MSNEIPNFNLLAVFSAVMEQGSLSKAADQLSTNQSTISTALARLKKEIGQELFVRKGRGVTPTSYAQSLYEQIKTPIHEL
NDVFQSMANFDEQSSERKFVISSPEHLQWILLNSFAALPNQNLSLEVFDQPDSDERIYEDLLTQEFDAMIDIVVPEHPSI
VSETLYEGEFVIVCREDHPRIQGEISEAQFMNEKHAVLDRTRRKVRSLNHYTSLDLSKRKIVFHGRSLFSNILLCSQSDY
ITVVPLSMAVQFQERLGLQLFKPPFEYQPISHYLIWLKKQNNDPAHKWFREQVISTSDAMSKTLKDRRLRF
>Mature_310_residues
SNEIPNFNLLAVFSAVMEQGSLSKAADQLSTNQSTISTALARLKKEIGQELFVRKGRGVTPTSYAQSLYEQIKTPIHELN
DVFQSMANFDEQSSERKFVISSPEHLQWILLNSFAALPNQNLSLEVFDQPDSDERIYEDLLTQEFDAMIDIVVPEHPSIV
SETLYEGEFVIVCREDHPRIQGEISEAQFMNEKHAVLDRTRRKVRSLNHYTSLDLSKRKIVFHGRSLFSNILLCSQSDYI
TVVPLSMAVQFQERLGLQLFKPPFEYQPISHYLIWLKKQNNDPAHKWFREQVISTSDAMSKTLKDRRLRF

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=290, Percent_Identity=28.9655172413793, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1786820, Length=298, Percent_Identity=23.1543624161074, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081766, Length=240, Percent_Identity=25, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35916; Mature: 35785

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIPNFNLLAVFSAVMEQGSLSKAADQLSTNQSTISTALARLKKEIGQELFVRKGRGV
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TPTSYAQSLYEQIKTPIHELNDVFQSMANFDEQSSERKFVISSPEHLQWILLNSFAALPN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCC
QNLSLEVFDQPDSDERIYEDLLTQEFDAMIDIVVPEHPSIVSETLYEGEFVIVCREDHPR
CCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCEEEEECCCCCC
IQGEISEAQFMNEKHAVLDRTRRKVRSLNHYTSLDLSKRKIVFHGRSLFSNILLCSQSDY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCC
ITVVPLSMAVQFQERLGLQLFKPPFEYQPISHYLIWLKKQNNDPAHKWFREQVISTSDAM
EEEEHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
SKTLKDRRLRF
HHHHHHHCCCC
>Mature Secondary Structure 
SNEIPNFNLLAVFSAVMEQGSLSKAADQLSTNQSTISTALARLKKEIGQELFVRKGRGV
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TPTSYAQSLYEQIKTPIHELNDVFQSMANFDEQSSERKFVISSPEHLQWILLNSFAALPN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCC
QNLSLEVFDQPDSDERIYEDLLTQEFDAMIDIVVPEHPSIVSETLYEGEFVIVCREDHPR
CCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCEEEEECCCCCC
IQGEISEAQFMNEKHAVLDRTRRKVRSLNHYTSLDLSKRKIVFHGRSLFSNILLCSQSDY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCC
ITVVPLSMAVQFQERLGLQLFKPPFEYQPISHYLIWLKKQNNDPAHKWFREQVISTSDAM
EEEEHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
SKTLKDRRLRF
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]