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The map label for this gene is gloA [H]

Identifier: 218710075

GI number: 218710075

Start: 2248879

End: 2249256

Strand: Reverse

Name: gloA [H]

Synonym: VS_2096

Alternate gene names: 218710075

Gene position: 2249256-2248879 (Counterclockwise)

Preceding gene: 218710076

Following gene: 218710074

Centisome position: 68.17

GC content: 47.35

Gene sequence:

>378_bases
ATGAAATTCCTACACACAATGATCCGAGTTGCTGATTTAGACAAGTCTATCGAGTTCTACACCAAGGTATTGGGAATGAA
GGAACTTGAACGCCACGAAAACAACGACTACCGCTACACATTGGTTTTCGTTGGATACGAACAAGGCGGCACGACTATCG
AGCTAACGTACAACTGGGATACTAACGAGTACGAGATGGGCAGCGCGTTTGGTCATTTGGCATTGGGTGTGGAAGATATT
TACGCCGCATGCGACAAGATCAAATCACTGGGTGGCAACGTCACTCGTGAAGCGGGGCCAGTAAAAGGTGGCTCAACGCA
CATCGCATTTATCACAGACCCAGATGGCTACCAAATCGAACTGATTCAACTGGGTTAA

Upstream 100 bases:

>100_bases
AATTCCTCGGTGAATCGTATTATTTCACTCAGAGATTAGTGGATAACCTCCACTCAAAATCATAACGGCGCCATGCTTGT
GGTGCCAATAAGGGTGAAAT

Downstream 100 bases:

>100_bases
TCGAGGATATAACAATGACAAACGCACTATTTCAACCAATTCAACTTGGTAGTATCGAACTTAAAAACCGTATTGTTATG
CCACCGATGACGCGTTCTCG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MKFLHTMIRVADLDKSIEFYTKVLGMKELERHENNDYRYTLVFVGYEQGGTTIELTYNWDTNEYEMGSAFGHLALGVEDI
YAACDKIKSLGGNVTREAGPVKGGSTHIAFITDPDGYQIELIQLG

Sequences:

>Translated_125_residues
MKFLHTMIRVADLDKSIEFYTKVLGMKELERHENNDYRYTLVFVGYEQGGTTIELTYNWDTNEYEMGSAFGHLALGVEDI
YAACDKIKSLGGNVTREAGPVKGGSTHIAFITDPDGYQIELIQLG
>Mature_125_residues
MKFLHTMIRVADLDKSIEFYTKVLGMKELERHENNDYRYTLVFVGYEQGGTTIELTYNWDTNEYEMGSAFGHLALGVEDI
YAACDKIKSLGGNVTREAGPVKGGSTHIAFITDPDGYQIELIQLG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=144, Percent_Identity=34.0277777777778, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787940, Length=125, Percent_Identity=62.4, Blast_Score=166, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=35.9154929577465, Blast_Score=84, Evalue=7e-18,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=36.3013698630137, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14047; Mature: 14047

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLHTMIRVADLDKSIEFYTKVLGMKELERHENNDYRYTLVFVGYEQGGTTIELTYNWD
CHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEECCC
TNEYEMGSAFGHLALGVEDIYAACDKIKSLGGNVTREAGPVKGGSTHIAFITDPDGYQIE
CCCEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEE
LIQLG
EEEEC
>Mature Secondary Structure
MKFLHTMIRVADLDKSIEFYTKVLGMKELERHENNDYRYTLVFVGYEQGGTTIELTYNWD
CHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEECCC
TNEYEMGSAFGHLALGVEDIYAACDKIKSLGGNVTREAGPVKGGSTHIAFITDPDGYQIE
CCCEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEE
LIQLG
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]