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The map label for this gene is bioB

Identifier: 218710020

GI number: 218710020

Start: 2185737

End: 2186789

Strand: Reverse

Name: bioB

Synonym: VS_2040

Alternate gene names: 218710020

Gene position: 2186789-2185737 (Counterclockwise)

Preceding gene: 218710023

Following gene: 218710019

Centisome position: 66.28

GC content: 47.29

Gene sequence:

>1053_bases
GTGGAAGTTCGTCATGACTGGACAGTTGCTGAAGTAAGAGCACTGCTTGAAAAACCGTTTATGGATTTAATGTTTGAAGC
TCAAGTGGTTCATAGACAGTACCAAGAGCACAACCACGTGCAGGTGAGTACACTGTTATCGATTAAGACAGGTGCTTGTC
CTGAAGATTGTAAGTATTGCCCTCAGAGTGCTCACTACCGAACGGATGTCGACAAAGAACGCTTAATGGAAGTCGAGCGT
GTTTTGGATGCGGCGCAAAAAGCTAAGAACGCGGGCTCGACTCGCTTCTGTATGGGCGCGGCGTGGAAAAACCCGAAAGA
ACGCGATATGCCTCACCTAACTGACATGATCAAAGGTGTGAAAGGCATGGGCCTAGAAACATGTATGACACTAGGCATGT
TAACGCCTGACCAAGCAGGTGAGCTAGCGGACGCAGGTTTGGATTACTACAACCATAACCTTGATACGTCTCCGGAGTTC
TACGGCAGTATTATCACCACTCGTACTTACCAAGACCGTTTAGATACGCTATCTCATGTGCGTGATGCCGGTATGAAGAT
CTGTTCTGGTGGTATTATTGGTATGGGCGAAAGTACTAATGACCGTGCAGGCCTGCTTGTAGAGCTTGCGAACCTTCCTG
TTCACCCAGAAAGTGTACCAATCAACATGCTCGTAAAAGTCAAAGGCACACCGATGGAAAACGTCGATGATGTTGAGTCT
TTCGACTTCGTCAAACTGATAGCGATTGCTCGTATCATGATGCCAATGTCTGCGGTTCGTTTATCAGCAGGCCGTGAGAA
CATGAACGAACAGATGCAAGCGATGTGTTTCATGGCGGGTGCGAATTCTATCTTCTACGGCTGTAAGCTACTGACTACGC
CAAACCCAGATGAAGACACGGACATGCAGCTGTTTAAGAAGTTAGGTATCAACAGCCAACAGGTGGCTCAGAAGCCTGAC
GAAATTCAAGAAAATGAACTGTTAGATCAAGTGGTGGAGCGGGTTGCTGCTCGTCCAACGAAAGATGACATGTTCTACGA
TGCCACTGTTTAA

Upstream 100 bases:

>100_bases
AATGTGTTGACAGTCTAAAGCTTGTCGGTAGACTATCCAAGCATAAATACAATCTCTATTGATAGAGATGTAACATCAAA
TAATAAAAAAGGATCTACAC

Downstream 100 bases:

>100_bases
ATCTCGCATCAAAAGTGCCCTTGTTCATCGCCGTGAGCAGGGGTTAACTCGTCAACTTAAGGTACTCGAAAACAGTAATG
GCCCTTTGCTTACTAGCGAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MEVRHDWTVAEVRALLEKPFMDLMFEAQVVHRQYQEHNHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDKERLMEVER
VLDAAQKAKNAGSTRFCMGAAWKNPKERDMPHLTDMIKGVKGMGLETCMTLGMLTPDQAGELADAGLDYYNHNLDTSPEF
YGSIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESTNDRAGLLVELANLPVHPESVPINMLVKVKGTPMENVDDVES
FDFVKLIAIARIMMPMSAVRLSAGRENMNEQMQAMCFMAGANSIFYGCKLLTTPNPDEDTDMQLFKKLGINSQQVAQKPD
EIQENELLDQVVERVAARPTKDDMFYDATV

Sequences:

>Translated_350_residues
MEVRHDWTVAEVRALLEKPFMDLMFEAQVVHRQYQEHNHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDKERLMEVER
VLDAAQKAKNAGSTRFCMGAAWKNPKERDMPHLTDMIKGVKGMGLETCMTLGMLTPDQAGELADAGLDYYNHNLDTSPEF
YGSIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESTNDRAGLLVELANLPVHPESVPINMLVKVKGTPMENVDDVES
FDFVKLIAIARIMMPMSAVRLSAGRENMNEQMQAMCFMAGANSIFYGCKLLTTPNPDEDTDMQLFKKLGINSQQVAQKPD
EIQENELLDQVVERVAARPTKDDMFYDATV
>Mature_350_residues
MEVRHDWTVAEVRALLEKPFMDLMFEAQVVHRQYQEHNHVQVSTLLSIKTGACPEDCKYCPQSAHYRTDVDKERLMEVER
VLDAAQKAKNAGSTRFCMGAAWKNPKERDMPHLTDMIKGVKGMGLETCMTLGMLTPDQAGELADAGLDYYNHNLDTSPEF
YGSIITTRTYQDRLDTLSHVRDAGMKICSGGIIGMGESTNDRAGLLVELANLPVHPESVPINMLVKVKGTPMENVDDVES
FDFVKLIAIARIMMPMSAVRLSAGRENMNEQMQAMCFMAGANSIFYGCKLLTTPNPDEDTDMQLFKKLGINSQQVAQKPD
EIQENELLDQVVERVAARPTKDDMFYDATV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=332, Percent_Identity=70.7831325301205, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=47.9495268138801, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_VIBSL (B7VH16)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417641.1
- ProteinModelPortal:   B7VH16
- SMR:   B7VH16
- GeneID:   7161595
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2040
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2300362
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39210; Mature: 39210

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRHDWTVAEVRALLEKPFMDLMFEAQVVHRQYQEHNHVQVSTLLSIKTGACPEDCKYC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHEEEECCCCCCHHHHHC
PQSAHYRTDVDKERLMEVERVLDAAQKAKNAGSTRFCMGAAWKNPKERDMPHLTDMIKGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
KGMGLETCMTLGMLTPDQAGELADAGLDYYNHNLDTSPEFYGSIITTRTYQDRLDTLSHV
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RDAGMKICSGGIIGMGESTNDRAGLLVELANLPVHPESVPINMLVKVKGTPMENVDDVES
HHHHHHHHCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHH
FDFVKLIAIARIMMPMSAVRLSAGRENMNEQMQAMCFMAGANSIFYGCKLLTTPNPDEDT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEECEEEECCCCCCCCH
DMQLFKKLGINSQQVAQKPDEIQENELLDQVVERVAARPTKDDMFYDATV
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MEVRHDWTVAEVRALLEKPFMDLMFEAQVVHRQYQEHNHVQVSTLLSIKTGACPEDCKYC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHEEEECCCCCCHHHHHC
PQSAHYRTDVDKERLMEVERVLDAAQKAKNAGSTRFCMGAAWKNPKERDMPHLTDMIKGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
KGMGLETCMTLGMLTPDQAGELADAGLDYYNHNLDTSPEFYGSIITTRTYQDRLDTLSHV
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RDAGMKICSGGIIGMGESTNDRAGLLVELANLPVHPESVPINMLVKVKGTPMENVDDVES
HHHHHHHHCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHH
FDFVKLIAIARIMMPMSAVRLSAGRENMNEQMQAMCFMAGANSIFYGCKLLTTPNPDEDT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEECEEEECCCCCCCCH
DMQLFKKLGINSQQVAQKPDEIQENELLDQVVERVAARPTKDDMFYDATV
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA