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The map label for this gene is bioF [H]

Identifier: 218710019

GI number: 218710019

Start: 2184539

End: 2185786

Strand: Reverse

Name: bioF [H]

Synonym: VS_2039

Alternate gene names: 218710019

Gene position: 2185786-2184539 (Counterclockwise)

Preceding gene: 218710020

Following gene: 218710018

Centisome position: 66.25

GC content: 46.55

Gene sequence:

>1248_bases
TTGCTGCTCGTCCAACGAAAGATGACATGTTCTACGATGCCACTGTTTAAATCTCGCATCAAAAGTGCCCTTGTTCATCG
CCGTGAGCAGGGGTTAACTCGTCAACTTAAGGTACTCGAAAACAGTAATGGCCCTTTGCTTACTAGCGAAGGTTCTAGCT
TCATTAATTTTTCGAGTAATGATTACTTAGGCTTGGCAAACGATTCTGAATTGGTTGATGCGTGGCAAGTAGGGCTTTCC
CAGTATGGTGCTGGTAGTGCGGCATCGCCATTGGTCACGGGTTTTAGTCCTGCTCATCGAAATTTAGAGACTCAGCTGTG
TGAATGGCTTGGCTTTGAACGCGCCATTCTCTTTAACTCTGGTTTCAGTGCTAACCAAGCTTTGTTGTTTTCTCTGCTCG
AAAAAGACGACTTTCTGTTGCAAGACAAACTTAACCACGCCTCTTTGATGGAAGCGGGGATGCTTTCACCTGCAACCATG
AAACGCTTTAAGCACAACGACACGCAACATTTAGAGTCGCTATTGAGTCAAACTCCACAATCCTTGGTCGTGACTGAGGG
TGTATTCAGCATGGATGGTGATCAAGCGCCTCTCCGTCAGATATCTAGCCTGACTAATCAATACGACAGTTGGTTGGCGG
TTGATGATGCCCACGGTATTGGCGTATTAGGTGATAACGGGGCAGGGAGTTGCAACGCCGCACAAATCTCACCTGATATC
TTGGTGGTGACCTTTGGTAAAGCATTTGGCCTTTCTGGTGCTGCGATTCTTTGTTCGTCAGAAGTGGGTGATTACTTAAC
TCAGTTTGCGCGTCATCATGTGTATTCGACGGCAATGCCACCTTCACAAGCGGTGGCTTTGTCTCATGCATGTCAGATGA
TTCAAACGCAAGAGTGGCGTCGTGAGAAACTCACGGAGCTAGGTGCGCTCTATGCAGAGCAGATGAATGGTGTGAAAGGT
TTTATTGATACTCAGACGCCGATCAAGCCATTTTTAATCGGCGAAGCCCAAGCAGCACTATCTATTGCTGAAGAGTTAAA
GCGCAACCAAGTTTGGGTAACGGCAATAAGGTCACCAACGGTGCCTATTGGGACTGCTCGTTTGCGTATTACCTTAACGG
CGAACCATAGTCAGGAACAGATTCTTCAGTTAACTGACTCGTTGCTTCAAGCAATAGAGCGAAGCAACGACTCTGGAAGC
AAACCAAGCATAGAATCAAGCTTTGAATTAAAGAAAGAGGCTCAGTAA

Upstream 100 bases:

>100_bases
ATGCAGCTGTTTAAGAAGTTAGGTATCAACAGCCAACAGGTGGCTCAGAAGCCTGACGAAATTCAAGAAAATGAACTGTT
AGATCAAGTGGTGGAGCGGG

Downstream 100 bases:

>100_bases
ATGTCACAAGAAGCAGTAGTGCATAATTACGTAAGCCAAGATAAGAGTGCGATTGCTGAAGCCTTCGGCAAAGCAGCTGC
AACCTATGACAAACACGCTG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLLVQRKMTCSTMPLFKSRIKSALVHRREQGLTRQLKVLENSNGPLLTSEGSSFINFSSNDYLGLANDSELVDAWQVGLS
QYGAGSAASPLVTGFSPAHRNLETQLCEWLGFERAILFNSGFSANQALLFSLLEKDDFLLQDKLNHASLMEAGMLSPATM
KRFKHNDTQHLESLLSQTPQSLVVTEGVFSMDGDQAPLRQISSLTNQYDSWLAVDDAHGIGVLGDNGAGSCNAAQISPDI
LVVTFGKAFGLSGAAILCSSEVGDYLTQFARHHVYSTAMPPSQAVALSHACQMIQTQEWRREKLTELGALYAEQMNGVKG
FIDTQTPIKPFLIGEAQAALSIAEELKRNQVWVTAIRSPTVPIGTARLRITLTANHSQEQILQLTDSLLQAIERSNDSGS
KPSIESSFELKKEAQ

Sequences:

>Translated_415_residues
MLLVQRKMTCSTMPLFKSRIKSALVHRREQGLTRQLKVLENSNGPLLTSEGSSFINFSSNDYLGLANDSELVDAWQVGLS
QYGAGSAASPLVTGFSPAHRNLETQLCEWLGFERAILFNSGFSANQALLFSLLEKDDFLLQDKLNHASLMEAGMLSPATM
KRFKHNDTQHLESLLSQTPQSLVVTEGVFSMDGDQAPLRQISSLTNQYDSWLAVDDAHGIGVLGDNGAGSCNAAQISPDI
LVVTFGKAFGLSGAAILCSSEVGDYLTQFARHHVYSTAMPPSQAVALSHACQMIQTQEWRREKLTELGALYAEQMNGVKG
FIDTQTPIKPFLIGEAQAALSIAEELKRNQVWVTAIRSPTVPIGTARLRITLTANHSQEQILQLTDSLLQAIERSNDSGS
KPSIESSFELKKEAQ
>Mature_415_residues
MLLVQRKMTCSTMPLFKSRIKSALVHRREQGLTRQLKVLENSNGPLLTSEGSSFINFSSNDYLGLANDSELVDAWQVGLS
QYGAGSAASPLVTGFSPAHRNLETQLCEWLGFERAILFNSGFSANQALLFSLLEKDDFLLQDKLNHASLMEAGMLSPATM
KRFKHNDTQHLESLLSQTPQSLVVTEGVFSMDGDQAPLRQISSLTNQYDSWLAVDDAHGIGVLGDNGAGSCNAAQISPDI
LVVTFGKAFGLSGAAILCSSEVGDYLTQFARHHVYSTAMPPSQAVALSHACQMIQTQEWRREKLTELGALYAEQMNGVKG
FIDTQTPIKPFLIGEAQAALSIAEELKRNQVWVTAIRSPTVPIGTARLRITLTANHSQEQILQLTDSLLQAIERSNDSGS
KPSIESSFELKKEAQ

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=372, Percent_Identity=30.1075268817204, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI284448556, Length=351, Percent_Identity=30.7692307692308, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI119220554, Length=470, Percent_Identity=28.2978723404255, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4758668, Length=376, Percent_Identity=29.2553191489362, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4502025, Length=344, Percent_Identity=29.6511627906977, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI40316939, Length=344, Percent_Identity=29.6511627906977, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI83977442, Length=349, Percent_Identity=30.945558739255, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI83977444, Length=346, Percent_Identity=30.3468208092486, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI83977440, Length=346, Percent_Identity=30.3468208092486, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI5454084, Length=247, Percent_Identity=24.6963562753036, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1786993, Length=353, Percent_Identity=51.5580736543909, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1790046, Length=384, Percent_Identity=33.59375, Blast_Score=224, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI71994529, Length=401, Percent_Identity=31.6708229426434, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI32566772, Length=373, Percent_Identity=27.0777479892761, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560912, Length=373, Percent_Identity=28.1501340482574, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17560914, Length=373, Percent_Identity=28.1501340482574, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71982625, Length=293, Percent_Identity=23.5494880546075, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71982617, Length=293, Percent_Identity=23.5494880546075, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320438, Length=374, Percent_Identity=31.8181818181818, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320267, Length=377, Percent_Identity=27.0557029177719, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6323954, Length=277, Percent_Identity=25.2707581227437, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24662918, Length=381, Percent_Identity=31.496062992126, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI17137420, Length=357, Percent_Identity=30.2521008403361, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17136286, Length=385, Percent_Identity=27.5324675324675, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653280, Length=266, Percent_Identity=24.4360902255639, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653276, Length=266, Percent_Identity=24.4360902255639, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653278, Length=266, Percent_Identity=24.4360902255639, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 45305; Mature: 45305

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVQRKMTCSTMPLFKSRIKSALVHRREQGLTRQLKVLENSNGPLLTSEGSSFINFSSN
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCC
DYLGLANDSELVDAWQVGLSQYGAGSAASPLVTGFSPAHRNLETQLCEWLGFERAILFNS
CEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCEEEEECC
GFSANQALLFSLLEKDDFLLQDKLNHASLMEAGMLSPATMKRFKHNDTQHLESLLSQTPQ
CCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCC
SLVVTEGVFSMDGDQAPLRQISSLTNQYDSWLAVDDAHGIGVLGDNGAGSCNAAQISPDI
CEEEECCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCCCCCCCCCCCCE
LVVTFGKAFGLSGAAILCSSEVGDYLTQFARHHVYSTAMPPSQAVALSHACQMIQTQEWR
EEEEECHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
REKLTELGALYAEQMNGVKGFIDTQTPIKPFLIGEAQAALSIAEELKRNQVWVTAIRSPT
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCC
VPIGTARLRITLTANHSQEQILQLTDSLLQAIERSNDSGSKPSIESSFELKKEAQ
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MLLVQRKMTCSTMPLFKSRIKSALVHRREQGLTRQLKVLENSNGPLLTSEGSSFINFSSN
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCC
DYLGLANDSELVDAWQVGLSQYGAGSAASPLVTGFSPAHRNLETQLCEWLGFERAILFNS
CEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCEEEEECC
GFSANQALLFSLLEKDDFLLQDKLNHASLMEAGMLSPATMKRFKHNDTQHLESLLSQTPQ
CCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCC
SLVVTEGVFSMDGDQAPLRQISSLTNQYDSWLAVDDAHGIGVLGDNGAGSCNAAQISPDI
CEEEECCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCCCCCCCCCCCCE
LVVTFGKAFGLSGAAILCSSEVGDYLTQFARHHVYSTAMPPSQAVALSHACQMIQTQEWR
EEEEECHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
REKLTELGALYAEQMNGVKGFIDTQTPIKPFLIGEAQAALSIAEELKRNQVWVTAIRSPT
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCC
VPIGTARLRITLTANHSQEQILQLTDSLLQAIERSNDSGSKPSIESSFELKKEAQ
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA