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The map label for this gene is bioD [H]

Identifier: 218710017

GI number: 218710017

Start: 2183055

End: 2183759

Strand: Reverse

Name: bioD [H]

Synonym: VS_2037

Alternate gene names: 218710017

Gene position: 2183759-2183055 (Counterclockwise)

Preceding gene: 218710018

Following gene: 218710015

Centisome position: 66.19

GC content: 45.67

Gene sequence:

>705_bases
TTGTTTAGGGGTTATTCAATTATGATTGATGCATTATTCATTGCAGGTACGGATACCGAAGTGGGAAAAACTGTGGTTTC
AAAAGCGATTCTTCAAGCTTTGGCCGCACAAGATTTATCAACAATTGGCTACAAGCCAGTTGCTGCGGGGTGTGAACAAT
ACCCTGAAGGGTTACGTAACAGTGACGCGCTGCATCTTCAAGAAGCGGCGACGCAAGATGTTGCCTACGAAGACGTTAAC
CCGTACGCGTTGCTACTACCATCATCACCGCATATCGCAGCTAAGCATGATGGTGTGGTGATTGATGAAACCGTATTATC
AGCTAAGTTGGCAGAGCATAAGCAAAACTCTGACATCGTGTTAGTTGAAGGTGCTGGTGGTTGGCGTGTGCCGGTTTCTG
ATGAAGAATATTTGTCTAGCTGGGTCAAAAAAGAGCAGCTGCCAGTGGTATTGACGGTCGGTATTAAGCTTGGCTGTTTG
AGTCATGCACTGCTAACCGCAGAAGTGATTCGTGCTGATGGACTGAACCTAGTGGGTTGGGTTGCAAACCGCATTAACCC
AGGTACTGAACATTACGCTGAAATTATAGCGATGCTTGAAGATAAGCTAGGTGCACCGAAGTTAGGTGAAATTCCGTATG
TGCCAAGAGCAAAGTCTAAGAACATTGGTAAGTACATTGATGTGCAGCCTTTGCTTGCGCTTTAA

Upstream 100 bases:

>100_bases
GCTCACAAGGTTTAACAAGCCGCCGCATGTTGCAGTTTGTTGAACGGGAATATCGAGAGTTTAAAAACCATCAAGGTTTC
TTGCCAGCAACATATCAAGT

Downstream 100 bases:

>100_bases
TTCTCACCTTGTGTACTAAATAAGAAAAGGGCTGCGATTGCAGCCCTTTTGCTATGTTAAGCCTTTTGGCCATGAGTTTA
TAAACAACTGGCCTTTGAAC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MFRGYSIMIDALFIAGTDTEVGKTVVSKAILQALAAQDLSTIGYKPVAAGCEQYPEGLRNSDALHLQEAATQDVAYEDVN
PYALLLPSSPHIAAKHDGVVIDETVLSAKLAEHKQNSDIVLVEGAGGWRVPVSDEEYLSSWVKKEQLPVVLTVGIKLGCL
SHALLTAEVIRADGLNLVGWVANRINPGTEHYAEIIAMLEDKLGAPKLGEIPYVPRAKSKNIGKYIDVQPLLAL

Sequences:

>Translated_234_residues
MFRGYSIMIDALFIAGTDTEVGKTVVSKAILQALAAQDLSTIGYKPVAAGCEQYPEGLRNSDALHLQEAATQDVAYEDVN
PYALLLPSSPHIAAKHDGVVIDETVLSAKLAEHKQNSDIVLVEGAGGWRVPVSDEEYLSSWVKKEQLPVVLTVGIKLGCL
SHALLTAEVIRADGLNLVGWVANRINPGTEHYAEIIAMLEDKLGAPKLGEIPYVPRAKSKNIGKYIDVQPLLAL
>Mature_234_residues
MFRGYSIMIDALFIAGTDTEVGKTVVSKAILQALAAQDLSTIGYKPVAAGCEQYPEGLRNSDALHLQEAATQDVAYEDVN
PYALLLPSSPHIAAKHDGVVIDETVLSAKLAEHKQNSDIVLVEGAGGWRVPVSDEEYLSSWVKKEQLPVVLTVGIKLGCL
SHALLTAEVIRADGLNLVGWVANRINPGTEHYAEIIAMLEDKLGAPKLGEIPYVPRAKSKNIGKYIDVQPLLAL

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=219, Percent_Identity=54.7945205479452, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1786995, Length=222, Percent_Identity=50.9009009009009, Blast_Score=223, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25193; Mature: 25193

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRGYSIMIDALFIAGTDTEVGKTVVSKAILQALAAQDLSTIGYKPVAAGCEQYPEGLRN
CCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
SDALHLQEAATQDVAYEDVNPYALLLPSSPHIAAKHDGVVIDETVLSAKLAEHKQNSDIV
CCCCHHHHHHHHHCHHCCCCCEEEEECCCCCEEECCCCEEEHHHHHHHHHHHHCCCCCEE
LVEGAGGWRVPVSDEEYLSSWVKKEQLPVVLTVGIKLGCLSHALLTAEVIRADGLNLVGW
EEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHH
VANRINPGTEHYAEIIAMLEDKLGAPKLGEIPYVPRAKSKNIGKYIDVQPLLAL
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCC
>Mature Secondary Structure
MFRGYSIMIDALFIAGTDTEVGKTVVSKAILQALAAQDLSTIGYKPVAAGCEQYPEGLRN
CCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
SDALHLQEAATQDVAYEDVNPYALLLPSSPHIAAKHDGVVIDETVLSAKLAEHKQNSDIV
CCCCHHHHHHHHHCHHCCCCCEEEEECCCCCEEECCCCEEEHHHHHHHHHHHHCCCCCEE
LVEGAGGWRVPVSDEEYLSSWVKKEQLPVVLTVGIKLGCLSHALLTAEVIRADGLNLVGW
EEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHH
VANRINPGTEHYAEIIAMLEDKLGAPKLGEIPYVPRAKSKNIGKYIDVQPLLAL
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA